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Gene information
General information
TC001043 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10148561..10150525
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001043
    iBeetle screen iB_03124

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva partially partiallynot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001043 RA
      MCPIDKLII GVLIVSFSS ISHCHNKYS AEANVKKSN 
      DDVDFRPVS LKHLDKPFR MAKLNLLWS KAVVRLSEP 
      KLKSLFGEL KLHDKEEIT WKHLKAEGK DKDGLKEAE 
      LRKKLLTIM GSYNLLDQA DDVQDPSKY RAHKPLNEA 
      SDKYINKSL FKDKKLNKL WEKAETAGF TAEELAALK 
      EEFTHHQDK VDQYYALLH DVKGKEQDD NTANSVDDG 
      LDKFNTIES LEEPQANKD YLDKVNLLR EQHKDIRDG 
      YDRLHLLAA KGPNSKEFV EPKVQGLWK IAIESNFKP 
      DELESLRVE LLHYENRLL KLRHLQAEA ALKEDLHKK 
      KMEMAGGKT NGMQMMEDT IKKHARKVE KIHLDLETR 
      IMQRHIEL
    CDS sequence
    • >TC001043 RA
      atgtgtcct atcgataag ttgataatt ggtgtgtta 
      attgttagt ttttcgtcg atttctcat tgtcacaat 
      aaatattcg gccgaagcg aatgtgaaa aaatcgaac 
      gatgatgtg gattttcgg ccggtgtcg ctgaagcac 
      ctggataag cctttcagg atggctaaa ttaaatctc 
      ttatggtcc aaggctgtg gtgaggctc tctgaacct 
      aaactaaaa tccctgttt ggcgaattg aaattgcac 
      gataaagag gaaatcact tggaagcat ttgaaagca 
      gaggggaag gacaaagac gggttaaaa gaggccgag 
      ttgaggaag aaattgctg acaataatg gggtcctat 
      aatttactg gaccaagcc gatgacgtg caagacccg 
      tctaaatat agagctcat aaacctctg aatgaggcc 
      tccgacaaa tacataaac aagagttta tttaaagat 
      aaaaagttg aacaaactg tgggaaaaa gccgagact 
      gctggtttt acggccgag gagttggcg gctttaaaa 
      gaggaattt acccaccac caggataaa gttgaccaa 
      tattacgct ttgttgcat gatgtcaaa ggcaaggaa 
      caagacgat aacactgcc aattcggta gatgatggt 
      ttagacaaa ttcaacacc attgaaagt ctggaagag 
      ccacaggca aacaaggat tatctggac aaagtgaac 
      ctcctcagg gagcagcac aaggatatc cgggacggc 
      tacgaccgc ttgcatttg ctggcggca aaggggccc 
      aacagtaaa gaatttgtg gaacccaaa gttcaaggt 
      ttatggaaa atcgcaatt gaaagtaac tttaaacct 
      gacgagttg gagtcgtta cgagtggag ctcctgcac 
      tatgaaaat aggttactt aaattgagg catcttcag 
      gctgaagct gccttgaaa gaagatctg cacaagaaa 
      aaaatggaa atggcaggg ggtaaaaca aacggaatg 
      cagatgatg gaagacacg attaaaaag catgcaagg 
      aaggtcgag aaaattcac ttggatctg gaaactagg 
      ataatgcag aggcatatt gaattgtga
    mRNA sequence
    • >TC001043 RA
      aattccata agggcaact taccattgc tggtacgta 
      tggaagtga aggaggtga tgccccact tgcccacag 
      tggctgcag accttgcgg cagatggtt tgttagcat 
      tttccttaa ataaattgt cgaaatgga aacttgcat 
      gtgaacttc ctccacggc gatacttcc cataccctc 
      ttgacattt ttaaaagtt gaactcaaa agtacccct 
      cactcaaac atttcggat taaaacttg agccctggc 
      aaaattcct ggcaataca aacgcgtcc ccaacgtcc 
      tatctagta atactatcg agttccatg atgttattt 
      tggcgattt atctgtaca ttttggtgt attttgtta 
      aaattggtt caacttcgc cattttcat caaatgatg 
      aaggtccat tgatattta ttaagtggg cttgcaggc 
      attggtaag gtgtcacag ataccacat tatttgtat 
      gagggtttc cacttttgg gaaaatgcg ggaaataat 
      taggttttt cttgtcaat agaattttc acgttataa 
      acacgccag taaataacg gctacgaaa tctacgtaa 
      ggttgcaca gttggctgt tgcaacgac gctcatctg 
      tcaaattta tacaaccgc ttgcaactg tcaaaaatt 
      ttcgtccaa cttttcaaa aataaccca aaaagatgt 
      gtcctatcg ataagttga taattggtg tgttaattg 
      ttagttttt cgtcgattt ctcattgtc acaataaat 
      attcggccg aagcgaatg tgaaaaaat cgaacgatg 
      atgtggatt ttcggccgg tgtcgctga agcacctgg 
      ataagcctt tcaggatgg ctaaattaa atctcttat 
      ggtccaagg ctgtggtga ggctctctg aacctaaac 
      taaaatccc tgtttggcg aattgaaat tgcacgata 
      aagaggaaa tcacttgga agcatttga aagcagagg 
      ggaaggaca aagacgggt taaaagagg ccgagttga 
      ggaagaaat tgctgacaa taatggggt cctataatt 
      tactggacc aagccgatg acgtgcaag acccgtcta 
      aatatagag ctcataaac ctctgaatg aggcctccg 
      acaaataca taaacaaga gtttattta aagataaaa 
      agttgaaca aactgtggg aaaaagccg agactgctg 
      gttttacgg ccgaggagt tggcggctt taaaagagg 
      aatttaccc accaccagg ataaagttg accaatatt 
      acgctttgt tgcatgatg tcaaaggca aggaacaag 
      acgataaca ctgccaatt cggtagatg atggtttag 
      acaaattca acaccattg aaagtctgg aagagccac 
      aggcaaaca aggattatc tggacaaag tgaacctcc 
      tcagggagc agcacaagg atatccggg acggctacg 
      accgcttgc atttgctgg cggcaaagg ggcccaaca 
      gtaaagaat ttgtggaac ccaaagttc aaggtttat 
      ggaaaatcg caattgaaa gtaacttta aacctgacg 
      agttggagt cgttacgag tggagctcc tgcactatg 
      aaaataggt tacttaaat tgaggcatc ttcaggctg 
      aagctgcct tgaaagaag atctgcaca agaaaaaaa 
      tggaaatgg cagggggta aaacaaacg gaatgcaga 
      tgatggaag acacgatta aaaagcatg caaggaagg 
      tcgagaaaa ttcacttgg atctggaaa ctaggataa 
      tgcagaggc atattgaat tgtgattat ctaattagt 
      gttgtaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY