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Gene information
General information
TC001026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9949619..9956255
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001026
    iBeetle screen iB_03122

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001026 RA
      MKDNESEEK EDRNVFKLL SQLRKEKLF ITNERLNIQ 
      KLNKSIKKK TDGLIEATW VTTKQRLYL LSQREFTTQ 
      NYEHIKSIT NSEFVEAKT LLGFQTVKI NNLLQNLRK 
      QPQELARWL FVGEQINDD QFSNVLQTV VTGLYSSCI 
      FPEDTTYML KLLLELAKL QLFKSENPR RMLKQGTCS 
      FKNLFFIFT EVLPTAKFF LSAALELPI LRLAACRDF 
      YLDIDPDKT LMRYRHNEN NLKSEFRDI QEYRTEVIR 
      KLSEFTNSF ISSLRENVY SFPKPIAWI VYHISKIIT 
      KSFGSKEAY AIVTELIFT LYICPAIVD PEQYCISDA 
      QITEVTQHN LMQVGQILQ VLALNKFES PENRFSDIY 
      NLLDNDVVC NFMEDILFN LEFDDDAPP VDLAPSIIR 
      DSALLAEDE LNNFILFLQ KVHNEMMQN DNFKKHLGD 
      NSIEEQIAS LILPSSLES SPKTSKPYT NGFSENTLK 
      RNHFLSLVT KNHTSKSRD EISQNIPLT VLIFSMEKD 
      LVEPVGLLP EEKILNMNL TAKKEVILP KPSEIITEA 
      VNGRVEEKP FSNFPDEGS IGNTSDNLE AISEAASNH 
      SVASSLELE NDQNDNLSD MISANVSGR GTPNISGRD 
      TPSSQVNDN EERPLPEAR QVEQPQPQI SRQIRSEID 
      DKFCKFEIK KLLEGDETI SIISETWST DVLASDNET 
      IDAGDSRAE RQGLQLVDQ AINEVPTGN ILDISETQS 
      ESAWSTDVM ASDTERLTE VDNDDAGSV AQSDETRSE 
      TDENPTRRL SNSSNYVNY LNYQEYRKV EIKNNVKTD 
      NNANNVLFK SVTLTDTQH NLSFSELKI ARKKDAPGP 
      SGFNPTELL KQNTYDERP NEILLSNCS LNSSSSGSS 
      SNSFENKHS HTETCWESK QWLNSSGSS LNVTSTPSE 
      STSELSVLS VSNLNPNIL VKKAATLNR SLKPSASTG 
      AIPKSISFD MSADKGDKY LDDDQRSKR GGFFGKLRM 
      GFRNRRGKS FRNQDDFRL DVEEFGAGK KTPNDSPVK 
      ISSSEVSED ILAKYRAKP FIESSPKEA NGNDKIPPK 
      VRSDDSTDN VDVNNSFDD IKRKLRLVL SNTTSQTPK 
      VIKENRLSV KSKVETILR LELGKARKL KEWSNIARI 
      SETLRFINL LNESTCLSL LQSLREDFL QRSVYTDYL 
      IYSRQELLF CQSYLDGLS EQVNNDKQQ CEKYLSMIC 
      VRKFLLDEE SRITQFCEE FKEINLFDE KCDFLNNFY 
      EQVYSVMQR SGLWQDIFK NQGDAIKIT LERCIMSKI 
      YKYALYPNG DGDRDRDHV LFQHIEKLA SIISPDHKA 
      LMIHKMFLR EMPWIPAQD ALKAMNAYK TPRDKVKCV 
      VHCAKCIMD LLAFSQNSG SMTADDFTP VLVYVIIRV 
      NPPDLLSTI QFVNSFYHS QIDGEELYW WTQFCSAVE 
      YIKTMDYSD
    CDS sequence
    • >TC001026 RA
      atgaaagat aatgagagt gaagaaaaa gaggacaga 
      aatgtcttc aaactgcta tcacaatta cgtaaagaa 
      aaattattc ataactaac gaaaggtta aatattcag 
      aaattgaac aaatcgata aaaaagaaa actgacggt 
      ttaatcgaa gccacatgg gtaacaaca aagcagcga 
      ttgtatctc ctctcccag cgagaattc acaacgcaa 
      aactacgaa catatcaag tcgatcacc aactcggaa 
      tttgtggaa gccaagact ttgttaggc ttccaaact 
      gttaaaatc aacaattta ctgcaaaac ctgcgaaag 
      cagcctcaa gaactagcc agatggctt ttcgtaggc 
      gagcaaata aatgacgat cagttttcc aatgtcttg 
      caaactgtg gtcactgga ttatactcc tcgtgcatc 
      ttccctgaa gataccact tacatgctg aaactttta 
      ctagaactc gcgaaattg cagcttttc aagagcgaa 
      aatcccaga cggatgctc aaacagggt acttgctcc 
      ttcaaaaat ttgtttttc atttttaca gaagtgttg 
      ccgactgct aaatttttc ctaagtgcc gcgctagaa 
      ttgcctatt ttgcggttg gcggcttgt agagatttt 
      tacctagac attgatcca gacaagaca ctcatgagg 
      tacagacac aacgagaat aatctcaaa tcggagttc 
      agggacatc caggaatac aggactgaa gttataaga 
      aaattatct gagttcaca aactcgttc atttcgtct 
      ttacgtgag aatgtttac agctttcct aaacccatt 
      gcgtggatt gtttaccac atttcgaaa attattacg 
      aaatctttt ggcagcaaa gaagcttat gctatcgtg 
      actgaatta attttcacg ctgtacatt tgcccagcc 
      atcgtcgat ccggaacag tactgtatc tcagacgca 
      caaatcacc gaagtcacc caacacaac ctgatgcaa 
      gtcgggcaa attcttcag gtcttggct ctgaataaa 
      ttcgagtcg cctgaaaac cgcttcagc gacatttac 
      aatttgttg gacaacgac gtagtttgc aatttcatg 
      gaagatatt ctctttaat ttggagttc gacgatgat 
      gcgccacct gttgatcta gcgccgagt ataatacgg 
      gactcggca cttttggcc gaggatgag ctgaacaat 
      tttatcttg tttttgcaa aaagtgcac aatgaaatg 
      atgcaaaat gataatttt aagaaacat ctcggggat 
      aattcgatt gaggaacaa atcgcctcg ctgattctc 
      ccatcgtcg cttgagagt tccccgaaa acgagcaaa 
      ccctacact aatggtttc tccgaaaat acgctgaag 
      aggaaccat ttcttgtcg ctggtcaca aaaaaccac 
      acgtcgaaa tcccgcgac gaaatctcg caaaacatc 
      cccttaact gtgttgatt ttctcaatg gagaaagac 
      ctggttgaa cctgtcggt cttttaccc gaagaaaaa 
      atcctcaat atgaatcta accgccaaa aaagaagtt 
      attttgccg aaaccatct gaaataatc acagaggcg 
      gtgaacggt cgcgttgag gaaaaaccc ttttcgaat 
      ttccccgac gaaggttcg atcggaaac acctcggac 
      aacctcgag gccatatcg gaggccgcc agcaaccat 
      tcggtggca agtagcctc gaattggaa aacgaccaa 
      aacgacaat ctttcggat atgatttcg gccaacgtt 
      tccggccgg ggaaccccc aacatttcc ggccgggac 
      acccccagc tcgcaggtg aacgataac gaagagcgc 
      ccgctaccc gaagcgcgc caagtcgag cagccccag 
      ccgcagata agtcggcaa attcggtca gaaatcgac 
      gacaagttt tgcaaattt gagataaaa aaattgctg 
      gaaggggac gaaacgatt tcgatcatt tccgaaact 
      tggagtact gatgttttg gcgtcggat aatgaaacg 
      atcgatgct ggggactcg agggccgag agacaaggc 
      ttgcagttg gtcgaccaa gcgataaat gaagtaccg 
      actgggaat atccttgat atttccgag actcagagc 
      gagagtgcc tggtcgact gatgtcatg gcttcggat 
      accgaacgc ttaaccgaa gtggataat gatgatgcc 
      ggaagtgtg gcacagtcc gacgaaacc cgatcggaa 
      accgacgaa aatccgacc cggagactg tcgaattcg 
      tccaattac gtcaactac ttgaactat caggagtac 
      cgaaaagtc gaaatcaaa aataacgta aaaaccgac 
      aataacgcc aataacgtg ttgtttaaa tcggtcact 
      ttgaccgac actcagcac aatttatcg ttttcggaa 
      ctgaaaatc gcccggaaa aaggacgct ccgggaccg 
      agcggtttt aacccaacc gaactcttg aaacaaaac 
      acgtacgat gagcgtccg aacgaaatc ctcctcagc 
      aattgcagc ctgaattcg agctcttcg ggcagtagt 
      agcaacagt ttcgaaaac aaacactca catactgaa 
      acttgctgg gaaagcaaa cagtggtta aacagttcg 
      ggaagtagt ttaaacgtc acatcgaca ccgtccgaa 
      agcacgagc gagttatca gtcctcagt gtcagtaat 
      ttgaatccg aatatttta gtaaagaaa gctgcgacg 
      ctgaatcgg agtttgaag ccctcggct tcgactggt 
      gctattccg aaaagtatt agttttgat atgagtgca 
      gataaaggt gataagtat ctcgatgac gatcagagg 
      agcaagagg ggaggcttt ttcgggaaa ttgagaatg 
      ggcttcagg aataggagg gggaagtcg ttccggaat 
      caggatgac ttccggttg gatgtcgag gagttcggg 
      gcggggaaa aagacgccc aatgactcg ccggttaag 
      atcagtagt tcagaagtg tcggaggac attttagcc 
      aaatatcgg gcgaaaccg ttcattgaa agtagcccg 
      aaagaagcc aacggcaat gataaaatc ccacctaaa 
      gtgagatct gacgattcc accgataac gtcgatgtt 
      aataatagc tttgatgat attaaaaga aaattacgt 
      ttagtttta agtaataca acttcacaa acccctaaa 
      gtaattaag gaaaaccga ttatctgta aaaagtaag 
      gttgaaacc atcctaaga ttagagtta ggcaaagct 
      cgtaagctg aaagaatgg tcaaacatc gcccgaatc 
      tctgaaacg ctgcgattt atcaattta ttaaatgag 
      agtacctgt ttgagctta ttacaatcg cttcgagaa 
      gattttttg caacgttcc gtctacacg gattattta 
      atatactcc cggcaggaa ctccttttt tgccaatca 
      taccttgac ggtttaagc gagcaagtt aacaacgat 
      aagcaacag tgcgagaaa tatttgagc atgatctgc 
      gtccgcaaa ttcctcctc gatgaggag agccgcatc 
      acccaattc tgcgaggaa ttcaaagaa atcaacctc 
      ttcgacgaa aagtgcgac tttttgaac aacttctac 
      gaacaagtc tactcagta atgcagcgc agtggctta 
      tggcaggac attttcaaa aaccagggt gatgcgatt 
      aaaatcact ctagagagg tgcattatg agcaaaatt 
      tacaagtac gccctgtac ccgaacggg gatggcgac 
      agggatcgc gaccatgtt ttgtttcaa catattgaa 
      aaattggcg agtattata agcccggac cataaagct 
      ttgatgatc cataagatg tttctgcgt gaaatgccc 
      tggattccg gcccaggac gccctcaag gcaatgaac 
      gcgtacaag acgccaaga gataaagtt aaatgtgtg 
      gttcattgt gcgaagtgt attatggat ttgctggcg 
      ttctcgcag aattcggga tcgatgact gcggacgat 
      ttcacccca gtgctggtc tatgttatt attagggtg 
      aatccgcct gatctgctg tcgactatc caattcgtc 
      aacagcttc taccacagc cagatcgac ggggaagag 
      ctctattgg tggacgcag ttctgctcc gctgttgag 
      tacataaaa acgatggat tattcggat tga
    mRNA sequence
    • >TC001026 RA
      catttgttg acaaatgtg tcaaaacaa agctgtatt 
      ttaagtgtt ttcgagctt taaagggct tcagagaaa 
      gtttttgta gtgcgactg atgtaaaaa accgtattt 
      ttgtaaagt caagtttta ggtctttag gatgaaaga 
      taatgagag tgaagaaaa agaggacag aaatgtctt 
      caaactgct atcacaatt acgtaaaga aaaattatt 
      cataactaa cgaaaggtt aaatattca gaaattgaa 
      caaatcgat aaaaaagaa aactgacgg tttaatcga 
      agccacatg ggtaacaac aaagcagcg attgtatct 
      cctctccca gcgagaatt cacaacgca aaactacga 
      acatatcaa gtcgatcac caactcgga atttgtgga 
      agccaagac tttgttagg cttccaaac tgttaaaat 
      caacaattt actgcaaaa cctgcgaaa gcagcctca 
      agaactagc cagatggct tttcgtagg cgagcaaat 
      aaatgacga tcagttttc caatgtctt gcaaactgt 
      ggtcactgg attatactc ctcgtgcat cttccctga 
      agataccac ttacatgct gaaactttt actagaact 
      cgcgaaatt gcagctttt caagagcga aaatcccag 
      acggatgct caaacaggg tacttgctc cttcaaaaa 
      tttgttttt catttttac agaagtgtt gccgactgc 
      taaattttt cctaagtgc cgcgctaga attgcctat 
      tttgcggtt ggcggcttg tagagattt ttacctaga 
      cattgatcc agacaagac actcatgag gtacagaca 
      caacgagaa taatctcaa atcggagtt cagggacat 
      ccaggaata caggactga agttataag aaaattatc 
      tgagttcac aaactcgtt catttcgtc tttacgtga 
      gaatgttta cagctttcc taaacccat tgcgtggat 
      tgtttacca catttcgaa aattattac gaaatcttt 
      tggcagcaa agaagctta tgctatcgt gactgaatt 
      aattttcac gctgtacat ttgcccagc catcgtcga 
      tccggaaca gtactgtat ctcagacgc acaaatcac 
      cgaagtcac ccaacacaa cctgatgca agtcgggca 
      aattcttca ggtcttggc tctgaataa attcgagtc 
      gcctgaaaa ccgcttcag cgacattta caatttgtt 
      ggacaacga cgtagtttg caatttcat ggaagatat 
      tctctttaa tttggagtt cgacgatga tgcgccacc 
      tgttgatct agcgccgag tataatacg ggactcggc 
      acttttggc cgaggatga gctgaacaa ttttatctt 
      gtttttgca aaaagtgca caatgaaat gatgcaaaa 
      tgataattt taagaaaca tctcgggga taattcgat 
      tgaggaaca aatcgcctc gctgattct cccatcgtc 
      gcttgagag ttccccgaa aacgagcaa accctacac 
      taatggttt ctccgaaaa tacgctgaa gaggaacca 
      tttcttgtc gctggtcac aaaaaacca cacgtcgaa 
      atcccgcga cgaaatctc gcaaaacat ccccttaac 
      tgtgttgat tttctcaat ggagaaaga cctggttga 
      acctgtcgg tcttttacc cgaagaaaa aatcctcaa 
      tatgaatct aaccgccaa aaaagaagt tattttgcc 
      gaaaccatc tgaaataat cacagaggc ggtgaacgg 
      tcgcgttga ggaaaaacc cttttcgaa tttccccga 
      cgaaggttc gatcggaaa cacctcgga caacctcga 
      ggccatatc ggaggccgc cagcaacca ttcggtggc 
      aagtagcct cgaattgga aaacgacca aaacgacaa 
      tctttcgga tatgatttc ggccaacgt ttccggccg 
      gggaacccc caacatttc cggccggga cacccccag 
      ctcgcaggt gaacgataa cgaagagcg cccgctacc 
      cgaagcgcg ccaagtcga gcagcccca gccgcagat 
      aagtcggca aattcggtc agaaatcga cgacaagtt 
      ttgcaaatt tgagataaa aaaattgct ggaagggga 
      cgaaacgat ttcgatcat ttccgaaac ttggagtac 
      tgatgtttt ggcgtcgga taatgaaac gatcgatgc 
      tggggactc gagggccga gagacaagg cttgcagtt 
      ggtcgacca agcgataaa tgaagtacc gactgggaa 
      tatccttga tatttccga gactcagag cgagagtgc 
      ctggtcgac tgatgtcat ggcttcgga taccgaacg 
      cttaaccga agtggataa tgatgatgc cggaagtgt 
      ggcacagtc cgacgaaac ccgatcgga aaccgacga 
      aaatccgac ccggagact gtcgaattc gtccaatta 
      cgtcaacta cttgaacta tcaggagta ccgaaaagt 
      cgaaatcaa aaataacgt aaaaaccga caataacgc 
      caataacgt gttgtttaa atcggtcac tttgaccga 
      cactcagca caatttatc gttttcgga actgaaaat 
      cgcccggaa aaaggacgc tccgggacc gagcggttt 
      taacccaac cgaactctt gaaacaaaa cacgtacga 
      tgagcgtcc gaacgaaat cctcctcag caattgcag 
      cctgaattc gagctcttc gggcagtag tagcaacag 
      tttcgaaaa caaacactc acatactga aacttgctg 
      ggaaagcaa acagtggtt aaacagttc gggaagtag 
      tttaaacgt cacatcgac accgtccga aagcacgag 
      cgagttatc agtcctcag tgtcagtaa tttgaatcc 
      gaatatttt agtaaagaa agctgcgac gctgaatcg 
      gagtttgaa gccctcggc ttcgactgg tgctattcc 
      gaaaagtat tagttttga tatgagtgc agataaagg 
      tgataagta tctcgatga cgatcagag gagcaagag 
      gggaggctt tttcgggaa attgagaat gggcttcag 
      gaataggag ggggaagtc gttccggaa tcaggatga 
      cttccggtt ggatgtcga ggagttcgg ggcggggaa 
      aaagacgcc caatgactc gccggttaa gatcagtag 
      ttcagaagt gtcggagga cattttagc caaatatcg 
      ggcgaaacc gttcattga aagtagccc gaaagaagc 
      caacggcaa tgataaaat cccacctaa agtgagatc 
      tgacgattc caccgataa cgtcgatgt taataatag 
      ctttgatga tattaaaag aaaattacg tttagtttt 
      aagtaatac aacttcaca aacccctaa agtaattaa 
      ggaaaaccg attatctgt aaaaagtaa ggttgaaac 
      catcctaag attagagtt aggcaaagc tcgtaagct 
      gaaagaatg gtcaaacat cgcccgaat ctctgaaac 
      gctgcgatt tatcaattt attaaatga gagtacctg 
      tttgagctt attacaatc gcttcgaga agatttttt 
      gcaacgttc cgtctacac ggattattt aatatactc 
      ccggcagga actcctttt ttgccaatc ataccttga 
      cggtttaag cgagcaagt taacaacga taagcaaca 
      gtgcgagaa atatttgag catgatctg cgtccgcaa 
      attcctcct cgatgagga gagccgcat cacccaatt 
      ctgcgagga attcaaaga aatcaacct cttcgacga 
      aaagtgcga ctttttgaa caacttcta cgaacaagt 
      ctactcagt aatgcagcg cagtggctt atggcagga 
      cattttcaa aaaccaggg tgatgcgat taaaatcac 
      tctagagag gtgcattat gagcaaaat ttacaagta 
      cgccctgta cccgaacgg ggatggcga cagggatcg 
      cgaccatgt tttgtttca acatattga aaaattggc 
      gagtattat aagcccgga ccataaagc tttgatgat 
      ccataagat gtttctgcg tgaaatgcc ctggattcc 
      ggcccagga cgccctcaa ggcaatgaa cgcgtacaa 
      gacgccaag agataaagt taaatgtgt ggttcattg 
      tgcgaagtg tattatgga tttgctggc gttctcgca 
      gaattcggg atcgatgac tgcggacga tttcacccc 
      agtgctggt ctatgttat tattagggt gaatccgcc 
      tgatctgct gtcgactat ccaattcgt caacagctt 
      ctaccacag ccagatcga cggggaaga gctctattg 
      gtggacgca gttctgctc cgctgttga gtacataaa 
      aacgatgga ttattcgga ttgacaaat gctgttcca 
      taataatct atttcttgt ataaacaat ttatgtatt 
      ttcagtaaa gtttacttt gtattaatt atctatttt 
      aaataagaa attgttaat tagtaaaat aagggggtt 
      cgaaataaa aacttcaca cgcat
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