Status
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Gene information
General information
TC001014 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9862900..9880425
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001014
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03119

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001014 RA
      MGPFDWHWL GFLVFFATC ATKADKIYT GLYSGELSQ 
      VHQTVPRKV GPGGEFISH NLTSFHDDG RVHFNVTLY 
      DREHLLVLE PVRNFLAPA LVIERRRRD TRTRSKPSD 
      KSKKCHFQG IVHGQPNSR VAVSACNGL TGMLHTEEG 
      RYYIEPSHH PLKQVDPGH PHLVYKRSA VTSAKRKKK 
      RKRKKHFRD CGTREPKKM TELEWQKQL GKVKVQEKK 
      HKTKHKIDI PSVQQSQMK KHKYKKVDV REKRSVSKP 
      HYVETLLVA DPTMVEFHE DGDIETYLL TIMNMVSSL 
      YMDPSIGNL IKVVVVKII LIDDPLSEP ELNVSVNAD 
      LTLKNFCKW QKNLNPDDD SHPHHHDVA ILITRKDIC 
      ARQNAPCNT LGVAHVAGM CKSSRSCSV NEDNGITLA 
      HTIAHEMGH NFGMYHDTE KVGCKGRQG NTSHIMTPS 
      FEADTVGVS WSRCSRRDI TNFLDKGLG LCLQDEPED 
      NEEYEYPEL PPGAMYNAD YQCRFQFGA KANVCSPLD 
      EICVRLWCL VNDTCTTQL RPAAPGTRC GKHKWCQDQ 
      KCVPIMEPP VAIDGGWGE WSSWSECSR TCGAGVSVT 
      QRECDHPTP TAGGRFCTG ERRRYRMCN TDPCPDNTP 
      TFRALQCSK HNNKTYEGK KYEWQPYFD QEEPCQLFC 
      SDANETVIV PWGEFAADG TPCNVGARD VCISGICRK 
      VGCDWVVDS TAQEDECGI CQGDGTKCD KIEGLYSKQ 
      SHSPGYREV VVIPPGARN IRIEEKDHS ENYISIGSA 
      LAKKFYLNG KRHITLPGE YTVAGAQAL YERDSQLEK 
      IRIPGPIRE PIVVYVIFR GKVHNPGVE YKYSISKRE 
      TTQKLKYTW IMGDWSQCS ATCGGGVQE RRPLCQEST 
      IRSVPSVVD GTSRIVDEV MCDETERLD KMVRTCNDD 
      PCPTHWWVG PWQSCPVTC SREGEKVLK RRSVMCVDG 
      QEMALPDRF CDRHVKPLE YTNCRALPT CD
    CDS sequence
    • >TC001014 RA
      atggggccc ttcgactgg cattggctc ggcttcctg 
      gttttcttc gccacttgc gcaaccaaa gccgacaag 
      atttataca ggtttatac agcggcgag ttgtcacaa 
      gtccaccaa acagttccc cgtaaagtg ggacctggg 
      ggcgaattc atctctcac aatttgaca agtttccac 
      gatgatggt cgcgtgcac ttcaacgtt acgctctac 
      gatcgggag catttattg gtgcttgaa ccggtccga 
      aatttttta gctccggct ctcgtaatc gagaggagg 
      agaagagac acacgcacc agatcgaaa ccgagcgat 
      aaatccaaa aagtgtcat tttcaaggc attgttcac 
      ggacagccg aattccagg gtggccgtt tcggcgtgt 
      aatggactg acaggtatg ttacacacg gaggagggt 
      agatattac atcgagccc tctcatcat ccgctgaaa 
      caagtggat ccggggcac ccccacctt gtgtacaag 
      cgttcggcc gtgacgagc gccaagaga aagaagaag 
      aggaaaaga aagaagcac tttcgggat tgcggcacc 
      cgcgagccg aagaaaatg accgagctg gaatggcag 
      aaacagctg ggaaaagtg aaggtgcaa gagaaaaag 
      cacaaaacc aaacataaa attgatatc ccgagcgta 
      cagcagtct caaatgaaa aaacataaa tataagaaa 
      gttgatgtg agggaaaag cgctcggtt agcaaaccg 
      cactacgtc gagactttg ctcgtcgct gatcccaca 
      atggtcgaa tttcacgaa gatggagac atcgaaact 
      tatttactc accatcatg aatatggtg tcgtccctg 
      tacatggat ccgagcatc ggaaatttg atcaaagtc 
      gttgttgtt aaaatcatt ttaatcgac gatccgctg 
      tccgagccg gaactgaac gtatccgtc aatgcagat 
      ctcacgttg aagaacttt tgtaaatgg caaaaaaat 
      ttaaatccg gacgacgac tcacatccg caccatcac 
      gacgttgct attcttatc acgaggaag gatatctgt 
      gctcgacag aacgcaccg tgtaatact ctaggagtt 
      gctcatgtc gccggaatg tgcaaaagc tcccggagc 
      tgcagcgtc aacgaggac aatggcatc accctggcc 
      cacaccatc gcgcacgag atgggacac aacttcgga 
      atgtaccac gacactgaa aaagtcggc tgcaaaggc 
      agacagggc aacacctcg cacatcatg actccgagc 
      ttcgaagcc gacaccgtg ggggtcagc tggtcccgc 
      tgtagccgt cgcgacatc accaacttt cttgacaag 
      ggactcgga ctgtgtctg caagacgag ccggaagat 
      aatgaagag tacgaatac ccggaactg ccccccgga 
      gcaatgtac aacgcggat taccagtgc cggtttcaa 
      tttggggcc aaggcgaat gtgtgctcg ccgctggat 
      gagatatgt gtgaggttg tggtgtctc gtaaatgac 
      acttgtacg acgcagctg aggccggca gcccccggg 
      acccgctgc ggaaagcac aagtggtgt caggatcag 
      aaatgcgtg ccgataatg gagcccccc gtggcgatc 
      gacggcgga tggggcgaa tggagttcc tggagtgaa 
      tgttctcgc acctgtggt gccggcgtg tccgtcaca 
      caaagagaa tgcgaccac ccgacacca acagccgga 
      ggtcgtttc tgcacggga gaacgaaga cgctaccgg 
      atgtgtaac accgacccg tgtcccgac aacaccccc 
      acgttccgc gccctccag tgctccaag cacaacaac 
      aagacctac gaggggaaa aagtacgag tggcagccc 
      tacttcgac caagaagag ccctgccag ttattctgc 
      agcgacgct aacgaaacg gtgatcgtg ccgtggggc 
      gagttcgcg gcggacggc accccctgc aacgtgggg 
      gcgcgcgat gtctgcatc tcggggatt tgcagaaaa 
      gtggggtgc gactgggtg gtggactcg acggcccag 
      gaagacgag tgcgggatc tgtcagggt gatgggacg 
      aaatgcgac aaaatcgaa ggcttgtac agcaaacag 
      agccactct ccggggtat cgagaagtt gtagtaata 
      ccaccgggt gcacggaat attcgcatc gaggagaag 
      gatcactcc gaaaactat atcagcatc gggagcgct 
      ctcgccaag aaattctat ctcaatgga aaacgtcac 
      atcacgtta ccgggcgaa tataccgtg gcaggggct 
      caagccctt tacgaaagg gacagccag ttggagaag 
      atccgaata ccgggcccg atacgagaa ccaatagtc 
      gtttacgtg attttcaga ggaaaagtc cacaacccg 
      ggcgtggag tacaagtac tcgatttca aaacgcgaa 
      accacccag aaactcaag tacacgtgg attatgggc 
      gactggtcc cagtgctcg gccacgtgc ggtggaggc 
      gtccaggag cgcagaccc ttgtgccag gagtctacc 
      atcaggagc gtcccatcg gtggtcgat gggacgtcc 
      aggatcgtg gacgaagta atgtgcgac gagacagaa 
      cgcctggat aaaatggtc aggacgtgc aatgacgat 
      ccttgtccc acccattgg tgggtgggt ccctggcag 
      agctgtcct gtgacctgc tccagggag ggagagaag 
      gtcctgaaa aggaggagt gttatgtgt gtggacggg 
      caggagatg gcgctgccc gatcgattt tgtgaccga 
      catgtgaag cccctggag tacaccaac tgcagggct 
      ctaccaact tgcgattaa
    mRNA sequence
    • >TC001014 RA
      actttctaa tggaattgc agttccgca aaatgcgtt 
      tcaattccc ggttgcaga gaattctgt ttacgaata 
      gtccgagtg tccgcttac acggccagg agtagaccg 
      tgaaaccgc tcgtaaagc attcgattc agatttccg 
      gaacaagtt tggcgacga tgggaagtg gcgatgcct 
      ccgtaggtg tttagtcat ctggtctgg agctgtgga 
      tctgctgct cactttgta ccaagacca agacaagtg 
      caggtgcaa cagtgcgcg atccggtga cctatttca 
      ggattacct atttgtgac gggtgacca ttttgttaa 
      tgcgagttg gtgacaatt tcgggatga tgaatttcc 
      gagtgttgt aagtatggg gcccttcga ctggcattg 
      gctcggctt cctggtttt cttcgccac ttgcgcaac 
      caaagccga caagattta tacaggttt atacagcgg 
      cgagttgtc acaagtcca ccaaacagt tccccgtaa 
      agtgggacc tgggggcga attcatctc tcacaattt 
      gacaagttt ccacgatga tggtcgcgt gcacttcaa 
      cgttacgct ctacgatcg ggagcattt attggtgct 
      tgaaccggt ccgaaattt tttagctcc ggctctcgt 
      aatcgagag gaggagaag agacacacg caccagatc 
      gaaaccgag cgataaatc caaaaagtg tcattttca 
      aggcattgt tcacggaca gccgaattc cagggtggc 
      cgtttcggc gtgtaatgg actgacagg tatgttaca 
      cacggagga gggtagata ttacatcga gccctctca 
      tcatccgct gaaacaagt ggatccggg gcaccccca 
      ccttgtgta caagcgttc ggccgtgac gagcgccaa 
      gagaaagaa gaagaggaa aagaaagaa gcactttcg 
      ggattgcgg cacccgcga gccgaagaa aatgaccga 
      gctggaatg gcagaaaca gctgggaaa agtgaaggt 
      gcaagagaa aaagcacaa aaccaaaca taaaattga 
      tatcccgag cgtacagca gtctcaaat gaaaaaaca 
      taaatataa gaaagttga tgtgaggga aaagcgctc 
      ggttagcaa accgcacta cgtcgagac tttgctcgt 
      cgctgatcc cacaatggt cgaatttca cgaagatgg 
      agacatcga aacttattt actcaccat catgaatat 
      ggtgtcgtc cctgtacat ggatccgag catcggaaa 
      tttgatcaa agtcgttgt tgttaaaat cattttaat 
      cgacgatcc gctgtccga gccggaact gaacgtatc 
      cgtcaatgc agatctcac gttgaagaa cttttgtaa 
      atggcaaaa aaatttaaa tccggacga cgactcaca 
      tccgcacca tcacgacgt tgctattct tatcacgag 
      gaaggatat ctgtgctcg acagaacgc accgtgtaa 
      tactctagg agttgctca tgtcgccgg aatgtgcaa 
      aagctcccg gagctgcag cgtcaacga ggacaatgg 
      catcaccct ggcccacac catcgcgca cgagatggg 
      acacaactt cggaatgta ccacgacac tgaaaaagt 
      cggctgcaa aggcagaca gggcaacac ctcgcacat 
      catgactcc gagcttcga agccgacac cgtgggggt 
      cagctggtc ccgctgtag ccgtcgcga catcaccaa 
      ctttcttga caagggact cggactgtg tctgcaaga 
      cgagccgga agataatga agagtacga atacccgga 
      actgccccc cggagcaat gtacaacgc ggattacca 
      gtgccggtt tcaatttgg ggccaaggc gaatgtgtg 
      ctcgccgct ggatgagat atgtgtgag gttgtggtg 
      tctcgtaaa tgacacttg tacgacgca gctgaggcc 
      ggcagcccc cgggacccg ctgcggaaa gcacaagtg 
      gtgtcagga tcagaaatg cgtgccgat aatggagcc 
      ccccgtggc gatcgacgg cggatgggg cgaatggag 
      ttcctggag tgaatgttc tcgcacctg tggtgccgg 
      cgtgtccgt cacacaaag agaatgcga ccacccgac 
      accaacagc cggaggtcg tttctgcac gggagaacg 
      aagacgcta ccggatgtg taacaccga cccgtgtcc 
      cgacaacac ccccacgtt ccgcgccct ccagtgctc 
      caagcacaa caacaagac ctacgaggg gaaaaagta 
      cgagtggca gccctactt cgaccaaga agagccctg 
      ccagttatt ctgcagcga cgctaacga aacggtgat 
      cgtgccgtg gggcgagtt cgcggcgga cggcacccc 
      ctgcaacgt gggggcgcg cgatgtctg catctcggg 
      gatttgcag aaaagtggg gtgcgactg ggtggtgga 
      ctcgacggc ccaggaaga cgagtgcgg gatctgtca 
      gggtgatgg gacgaaatg cgacaaaat cgaaggctt 
      gtacagcaa acagagcca ctctccggg gtatcgaga 
      agttgtagt aataccacc gggtgcacg gaatattcg 
      catcgagga gaaggatca ctccgaaaa ctatatcag 
      catcgggag cgctctcgc caagaaatt ctatctcaa 
      tggaaaacg tcacatcac gttaccggg cgaatatac 
      cgtggcagg ggctcaagc cctttacga aagggacag 
      ccagttgga gaagatccg aataccggg cccgatacg 
      agaaccaat agtcgttta cgtgatttt cagaggaaa 
      agtccacaa cccgggcgt ggagtacaa gtactcgat 
      ttcaaaacg cgaaaccac ccagaaact caagtacac 
      gtggattat gggcgactg gtcccagtg ctcggccac 
      gtgcggtgg aggcgtcca ggagcgcag acccttgtg 
      ccaggagtc taccatcag gagcgtccc atcggtggt 
      cgatgggac gtccaggat cgtggacga agtaatgtg 
      cgacgagac agaacgcct ggataaaat ggtcaggac 
      gtgcaatga cgatccttg tcccaccca ttggtgggt 
      gggtccctg gcagagctg tcctgtgac ctgctccag 
      ggagggaga gaaggtcct gaaaaggag gagtgttat 
      gtgtgtgga cgggcagga gatggcgct gcccgatcg 
      attttgtga ccgacatgt gaagcccct ggagtacac 
      caactgcag ggctctacc aacttgcga ttaagcatt 
      ttattgttg ctgtataaa ttaacctaa tgttattta 
      tgttttaga taagttttt aacatcaca atactcata 
      atgtaccat attttctgt ataggtata tggtgaaaa 
      atataagac tgattttgt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY