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Gene information
General information
TC000997 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4012585..4017702
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000997
    iBeetle screen iB_03117
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000997 RA
      MFGWGSTIH GELGLGGIE EEHVFTPKA LDWFEAKSV 
      MKAALGANH TLFLTKDGK VYSCGSNDY GQLGHDQPR 
      KRPQLVSGL DAYSIINVA CGEMHSLAL SRWGEVFSW 
      GSDNYGQLG HQLGGTLQI VPKLIKSLA KYQVVQIAA 
      GQRHSVALT NAGEIFVWG ANDFGQLGL GTITPYEST 
      PTTVCSLSG IPFALIACG ANHTFAVSK SGAVFGWGK 
      NINGQLGLN DTVSKKFPT QLRTLRNSR VRFISCGDE 
      FSTFLTLDG GVFSCGAGM YGQLGHGGT SNEILPRQV 
      LELMGSTIT QISCGRQHS LAFVPSRGR VYSYGLGGA 
      GQLGLRKPS NASTPQVVL GPWVSPSGV PVVPNNAPV 
      NTVINRIYA GGDHCFVSV TEKKAKIEP NDFRNYEPE 
      SQILTLRVD RLEPCAKVA VNGQVEQEI LAYLEVVFK 
      NLACWNASF LVAEHYYCT SKHHGVDME EAERAFSII 
      AKIEHETIK ELIWNHVVN DILGQLLPS PPDVETLRI 
      YLVLPLYHE FNNPKQHLK LHKPFASAL LKLGPQASK 
      VVGMWWTTM SCDYFEHLI RVFKSVVSF ILRNQRIPE 
      NKTVFYDPS LVAMLDTLT YLNKLNHSV EGLKVPYDT 
      FYISDLCDY LDVRVDYVM WLTDPNTGK LFLCNYPFI 
      FDAQAKTQL LETDQRIQM HKAMQSAAQ QAVLSMLFR 
      PAGVLSITQ FLVLNVTRD HIVQDALRE LSNVDPDDL 
      KKPLRVKFC GEEAEDAGG VTKEFFLLL LKEILDPKY 
      GMFKEYEET RALWFSENS FEEEPVYFL IGLICGLAI 
      YNFTIIDLP FPLALYKKL LNEPVGLAD LKGLSPMMA 
      NSLQSILDY NESDFHSLF DLCFEISRD EFGESRVVE 
      LKPNGSNIP VTQDNKNEY VNLYVDYIF NTSIKKQYD 
      AFHHGFMKV CGGRVLQLF HSHELQAVV VGNENYDWH 
      ALEEVAEYK NGYTSSDKT IRWFWEVIH EMPLEDKKK 
      FLLFLTGSD RIPLQGMKA IKIIIQPTT DDKYLPVAH 
      TCFNLLDLP RYSTKEKLK YKLMQAIQQ TQGFSLV
    CDS sequence
    • >TC000997 RA
      atgtttggt tggggaagt acaattcac ggtgaactg 
      ggcttgggg ggcattgaa gaggagcat gtcttcacc 
      cccaaggcc cttgactgg tttgaagca aaatctgtg 
      atgaaagct gccttaggt gcaaaccat acgctgttt 
      ttgaccaaa gacgggaaa gtgtattcc tgtggaagc 
      aacgactat ggacagttg ggccacgac cagcctaga 
      aaaaggcca caattggtc tctggtctt gacgcatac 
      tctatcata aatgtggcc tgtggcgaa atgcactct 
      ttggccttg agtcgttgg ggcgaagtt ttttcatgg 
      ggctcggac aattacggc cagctgggc caccaactg 
      gggggcact ttacaaatc gttcctaaa ctaatcaaa 
      tcgttagct aagtatcag gtcgttcaa atagcagca 
      gggcaacga cattcagta gctttaacc aatgctggg 
      gaaatattt gtttggggg gccaatgat ttcggacaa 
      ctgggtcta ggaacaata acgccatat gaatcaaca 
      ccaaccacc gtttgttct ttaagcggc attccgttt 
      gcgctaatt gcctgcggg gcgaaccac acttttgct 
      gtctcaaag tcgggggct gtcttcgga tggggaaaa 
      aacatcaac ggccagctc ggcttaaac gacactgtg 
      tcaaaaaaa ttccccact cagctgcga acactgcga 
      aacagtcgc gtcagattc atttcatgc ggtgacgaa 
      ttttccact tttctcacc ctcgacggc ggtgtgttt 
      tcctgtggg gccggaatg tacggccag ctgggccac 
      ggtggtacc tcaaacgag atcttacca cgacaagta 
      ttggaactg atgggttcg acaataact caaatttcc 
      tgcggtcgg caacactcg ttagccttc gtgccgtca 
      cgtggacgt gtctatagc tacggattg ggtggtgcc 
      ggccaattg ggtttgagg aaacccagt aacgcgtcc 
      acgccccag gttgtgctc gggccgtgg gtttccccg 
      agtggggtg ccggtggtt cccaacaat gcgccggta 
      aacaccgtg attaatcgg atttacgcc ggcggggac 
      cactgtttc gtttctgtg actgagaaa aaggcgaaa 
      attgagccg aacgatttt cgaaattac gagccggag 
      agccagatt ttgacgctg cgggtggat aggctggag 
      ccgtgcgcg aaagttgcg gtcaatggc caggtggag 
      caggagatt ttggcgtat ttggaggtg gtttttaag 
      aatctggcc tgttggaat gcctcgttt ctagtcgct 
      gagcattat tattgcacg agtaagcac cacggggtg 
      gatatggag gaggccgaa agggcgttt tccataatt 
      gcgaaaatt gagcacgaa acgatcaag gagctcatt 
      tggaatcac gttgttaac gatattttg ggccaactg 
      ttgccttcg cctcccgat gttgagacg ttgagaatt 
      tacctcgtt ttgcctctt tatcacgaa tttaataat 
      ccgaaacaa cacttaaag ttacataag ccattcgca 
      agtgccctt cttaagctg ggcccacag gcgagtaaa 
      gtcgttgga atgtggtgg acgacaatg agttgtgat 
      tattttgag catttgata cgcgttttc aagtccgtt 
      gtgtcgttc attttgaga aatcagaga attccggaa 
      aataagacc gttttttac gatcctagt ctcgtagcc 
      atgttagat accttaact tatttaaat aaacttaac 
      catagcgtg gaaggttta aaagttcca tacgataca 
      ttctatatc tcggatttg tgcgattat ttggacgtt 
      cgggtggat tacgtcatg tggctcacc gatccaaac 
      acggggaaa ctttttctg tgtaattac ccgtttatc 
      ttcgatgct caagccaag actcagctc ttggagacc 
      gatcagcgc attcagatg cataaagca atgcaaagc 
      gccgctcaa caagccgtc ttatccatg ttattccgg 
      ccggcgggc gttctaagt attacccaa tttctggtt 
      ttaaacgtc actagagat cacatagtg caagacgca 
      ctcagagag cttagtaac gtcgatcct gacgatttg 
      aaaaagcca cttcgggtg aaattttgt ggcgaagaa 
      gccgaggat gcgggcggt gtaaccaaa gagtttttc 
      ctcttgttg ttaaaagaa atactcgat ccgaaatac 
      ggcatgttc aaggagtat gaagagacg agagcattg 
      tggttttcg gaaaactcc ttcgaggag gagccggtt 
      tattttctt atagggctc atttgtgga ctggcgatt 
      tataatttt acgataatc gatttgccg tttcccttg 
      gctctctat aaaaaatta ctcaatgag ccggtaggg 
      ttggctgat ttgaaagga ctatcgccg atgatggca 
      aactccttg caaagtatt ctcgactat aacgagagc 
      gatttccat tcgttgttt gatttgtgc tttgagata 
      agtcgcgac gaatttggc gagtccaga gttgtggaa 
      ctcaagccg aacggatca aatattcca gtcacgcaa 
      gacaacaaa aatgagtat gtaaatctt tacgttgat 
      tatattttt aacacctcg attaagaaa caatacgat 
      gcatttcac cacggtttc atgaaggtt tgtggcggc 
      cgcgtactt caactgttt cactcgcat gagcttcaa 
      gctgttgtt gtcggtaat gaaaattac gattggcac 
      gctttggaa gaagtagca gagtataaa aacggatac 
      acctcgtct gataagaca ataaggtgg ttttgggaa 
      gttatccac gaaatgcct ttagaagac aaaaagaaa 
      tttctgctt tttttgact ggtagtgat aggattccg 
      cttcaagga atgaaagca atcaaaatt attatacaa 
      ccaacaact gacgataaa tacttgccg gtggcccac 
      acgtgtttc aatttgttg gacctcccg aggtacagc 
      accaaagaa aagctcaag tataagctg atgcaagcg 
      atccaacaa acgcaggga ttttcgtta gtctga
    mRNA sequence
    • >TC000997 RA
      agccgattc aacataacc ttcgcaaaa aattgtcac 
      ttttcaatc catcaaata aaaaatctg caaacattc 
      taatcgaac tcaaaattt gtaataatg taaattgga 
      gcattttag gctaaaacc gaattcaag cgactcgaa 
      agctctaga agaaatgtt tggttgggg aagtacaat 
      tcacggtga actgggctt ggggggcat tgaagagga 
      gcatgtctt cacccccaa ggcccttga ctggtttga 
      agcaaaatc tgtgatgaa agctgcctt aggtgcaaa 
      ccatacgct gtttttgac caaagacgg gaaagtgta 
      ttcctgtgg aagcaacga ctatggaca gttgggcca 
      cgaccagcc tagaaaaag gccacaatt ggtctctgg 
      tcttgacgc atactctat cataaatgt ggcctgtgg 
      cgaaatgca ctctttggc cttgagtcg ttggggcga 
      agttttttc atggggctc ggacaatta cggccagct 
      gggccacca actgggggg cactttaca aatcgttcc 
      taaactaat caaatcgtt agctaagta tcaggtcgt 
      tcaaatagc agcagggca acgacattc agtagcttt 
      aaccaatgc tggggaaat atttgtttg gggggccaa 
      tgatttcgg acaactggg tctaggaac aataacgcc 
      atatgaatc aacaccaac caccgtttg ttctttaag 
      cggcattcc gtttgcgct aattgcctg cggggcgaa 
      ccacacttt tgctgtctc aaagtcggg ggctgtctt 
      cggatgggg aaaaaacat caacggcca gctcggctt 
      aaacgacac tgtgtcaaa aaaattccc cactcagct 
      gcgaacact gcgaaacag tcgcgtcag attcatttc 
      atgcggtga cgaattttc cacttttct caccctcga 
      cggcggtgt gttttcctg tggggccgg aatgtacgg 
      ccagctggg ccacggtgg tacctcaaa cgagatctt 
      accacgaca agtattgga actgatggg ttcgacaat 
      aactcaaat ttcctgcgg tcggcaaca ctcgttagc 
      cttcgtgcc gtcacgtgg acgtgtcta tagctacgg 
      attgggtgg tgccggcca attgggttt gaggaaacc 
      cagtaacgc gtccacgcc ccaggttgt gctcgggcc 
      gtgggtttc cccgagtgg ggtgccggt ggttcccaa 
      caatgcgcc ggtaaacac cgtgattaa tcggattta 
      cgccggcgg ggaccactg tttcgtttc tgtgactga 
      gaaaaaggc gaaaattga gccgaacga ttttcgaaa 
      ttacgagcc ggagagcca gattttgac gctgcgggt 
      ggataggct ggagccgtg cgcgaaagt tgcggtcaa 
      tggccaggt ggagcagga gattttggc gtatttgga 
      ggtggtttt taagaatct ggcctgttg gaatgcctc 
      gtttctagt cgctgagca ttattattg cacgagtaa 
      gcaccacgg ggtggatat ggaggaggc cgaaagggc 
      gttttccat aattgcgaa aattgagca cgaaacgat 
      caaggagct catttggaa tcacgttgt taacgatat 
      tttgggcca actgttgcc ttcgcctcc cgatgttga 
      gacgttgag aatttacct cgttttgcc tctttatca 
      cgaatttaa taatccgaa acaacactt aaagttaca 
      taagccatt cgcaagtgc ccttcttaa gctgggccc 
      acaggcgag taaagtcgt tggaatgtg gtggacgac 
      aatgagttg tgattattt tgagcattt gatacgcgt 
      tttcaagtc cgttgtgtc gttcatttt gagaaatca 
      gagaattcc ggaaaataa gaccgtttt ttacgatcc 
      tagtctcgt agccatgtt agatacctt aacttattt 
      aaataaact taaccatag cgtggaagg tttaaaagt 
      tccatacga tacattcta tatctcgga tttgtgcga 
      ttatttgga cgttcgggt ggattacgt catgtggct 
      caccgatcc aaacacggg gaaactttt tctgtgtaa 
      ttacccgtt tatcttcga tgctcaagc caagactca 
      gctcttgga gaccgatca gcgcattca gatgcataa 
      agcaatgca aagcgccgc tcaacaagc cgtcttatc 
      catgttatt ccggccggc gggcgttct aagtattac 
      ccaatttct ggttttaaa cgtcactag agatcacat 
      agtgcaaga cgcactcag agagcttag taacgtcga 
      tcctgacga tttgaaaaa gccacttcg ggtgaaatt 
      ttgtggcga agaagccga ggatgcggg cggtgtaac 
      caaagagtt tttcctctt gttgttaaa agaaatact 
      cgatccgaa atacggcat gttcaagga gtatgaaga 
      gacgagagc attgtggtt ttcggaaaa ctccttcga 
      ggaggagcc ggtttattt tcttatagg gctcatttg 
      tggactggc gatttataa ttttacgat aatcgattt 
      gccgtttcc cttggctct ctataaaaa attactcaa 
      tgagccggt agggttggc tgatttgaa aggactatc 
      gccgatgat ggcaaactc cttgcaaag tattctcga 
      ctataacga gagcgattt ccattcgtt gtttgattt 
      gtgctttga gataagtcg cgacgaatt tggcgagtc 
      cagagttgt ggaactcaa gccgaacgg atcaaatat 
      tccagtcac gcaagacaa caaaaatga gtatgtaaa 
      tctttacgt tgattatat ttttaacac ctcgattaa 
      gaaacaata cgatgcatt tcaccacgg tttcatgaa 
      ggtttgtgg cggccgcgt acttcaact gtttcactc 
      gcatgagct tcaagctgt tgttgtcgg taatgaaaa 
      ttacgattg gcacgcttt ggaagaagt agcagagta 
      taaaaacgg atacacctc gtctgataa gacaataag 
      gtggttttg ggaagttat ccacgaaat gcctttaga 
      agacaaaaa gaaatttct gcttttttt gactggtag 
      tgataggat tccgcttca aggaatgaa agcaatcaa 
      aattattat acaaccaac aactgacga taaatactt 
      gccggtggc ccacacgtg tttcaattt gttggacct 
      cccgaggta cagcaccaa agaaaagct caagtataa 
      gctgatgca agcgatcca acaaacgca gggattttc 
      gttagtctg attcatgtg agctttatt tttccattg 
      ttaccaatt ttttatttt ttgtattaa attaagtag 
      tcacacgat tgacttttc ttattaata ttgtatgaa 
      aaacgctta accgttgca gcgcattta gcttgcgtc 
      acgaacatt gtgaaaaaa tcacacgta tttttctaa 
      gattgttgt gagttgaat atattttgt aaacgatg