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Gene information
General information
TC000981 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9173841..9210690
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000981
    iBeetle screen iB_03114

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000981 RA
      MGSNLVVVV VVVVVVVAS LSASARAYS WPSEETTTR 
      PPQARDFHS DPLVVETTS GLVRGKAKT VLGREVHVF 
      TGIPFAKPP IEQLRFRKP VPIDPWHGI LDATKLPNS 
      CYQERYEYF PGFEGEEMW NPNTNISED CLYLNIWVP 
      QRLRIRHHG EKLPQDRPK VPVLVWIYG GGYMSGTST 
      LDIYDADII AATSDVIVA SMQYRVGAF GFLYLSKYF 
      PRGSEEAPG NMGMWDQAL AIRWIKENA AAFGGDPDL 
      ITLFGESAG GGSVSILLL SPVTKGLAR RGILQSGTM 
      NAPWSYMSG ERAQQIGKV LVEDCGCNV SLLETRPHE 
      VIDCMRAVE AKTISLQQW NSYSGILGF PSTPTVDGV 
      FMPKHPMDM LAEGDYEDM EILVGSNQD EGTYFLLYD 
      FIDFFEKDG PSFLQRDKY HDIIDTIFK NMSRLERDA 
      IVFQYTDWE HVNDGYLNQ KMVGDVVGD YFFICPTND 
      FAELAAERG MKVYYYFFT HRTSTSLWG EWMGVMHGD 
      EIEYVFGHP LNMSLQFNS RERELSLKI MQAFARFAA 
      TGKPVTDDV NWPLYTKDQ PQYFIFNAD KNGIGKGPR 
      ATACAFWND FLPKLRDNP DSAESPCVN TYLSKMGSS 
      SGRASTRTL LEILIHLML ALSAL
    CDS sequence
    • >TC000981 RA
      atgggttcg aatctcgtc gtcgtcgtg gtcgtcgtg 
      gtggtggtg gtggcgtcg ctgagtgct tccgcccgc 
      gcctactcc tggcccagc gaggagacg acgacgcgt 
      ccgccccaa gccagagac tttcacagc gacccgctg 
      gtggtggag accaccagc ggcctggtg cggggcaag 
      gccaagacg gtgctgggc cgcgaggtg cacgtcttc 
      accgggatt cccttcgcc aagccgccc atcgaacag 
      ctgaggttc aggaagccc gtgcccatc gatccctgg 
      cacggcatc ttagatgcc accaagctg cccaactcg 
      tgctaccag gagcgctac gagtacttc cccggcttt 
      gagggcgag gagatgtgg aaccccaac acgaacatc 
      tccgaggac tgcctctac ctcaacatc tgggtgccc 
      cagcgcttg cgcatccgg caccacggc gagaagctc 
      ccccaggac cggcccaaa gtccccgtc ctcgtgtgg 
      atctacggc gggggctac atgagcggc acctccacc 
      ctggacatc tacgacgcc gacatcatc gccgccacc 
      tccgacgtc atcgtcgcc tccatgcaa tatcgcgtg 
      ggggcgttc ggcttcctc tacttgagc aagtacttc 
      cctcgcggc agtgaggag gcgcccggt aacatgggc 
      atgtgggac caagccctg gccatccgc tggatcaag 
      gagaacgcg gcggccttt gggggcgac ccagacctc 
      atcacgctg tttggggag tcggctgga ggcggctcc 
      gtcagcatc ctgctcctg agtccggtt actaaaggc 
      ctggccagg agggggatt ctgcagtcg gggactatg 
      aacgcccct tggagttac atgtcgggg gagagggcg 
      cagcagatc gggaaggtc ctggtggag gactgcggg 
      tgcaacgtc tccttgttg gagacgagg ccgcatgag 
      gtcattgat tgcatgagg gcggtggag gccaagacg 
      atttcgctg caacagtgg aattcgtat tcggggatt 
      ttgggcttc ccctcaacg cctacggtt gatggcgtc 
      ttcatgccc aagcatccc atggatatg ctggcggaa 
      ggggattac gaggatatg gagatcctg gtcgggagt 
      aaccaagat gaaggcact tacttctta ctttacgat 
      tttatcgat ttcttcgaa aaggatggc cctagcttc 
      ctccaacga gacaaatac cacgacatt atcgatacg 
      atattcaaa aatatgagt cggttggaa cgtgatgcc 
      atagtattt cagtatact gattgggag cacgtcaac 
      gacggctac ttgaaccag aaaatggtg ggcgacgtc 
      gtcggagat tattttttc atttgtcca accaacgat 
      ttcgccgag ctggcagca gagcgcgga atgaaagtc 
      tactattat tttttcaca cacaggaca agcacgtcg 
      ttgtggggc gaatggatg ggggtgatg cacggggat 
      gagatagaa tacgtgttt ggccatcct ttgaacatg 
      tcgttgcag tttaactca agggaacgg gaactcagt 
      ctgaagata atgcaagcc tttgccaga tttgcagca 
      acggggaaa ccagtgaca gacgacgtg aattggcca 
      ttgtacaca aaagaccaa ccgcagtat ttcatcttc 
      aacgccgac aaaaacggc atcggcaaa ggtcctcga 
      gcgacagcg tgcgcgttt tggaacgat ttcctgccc 
      aagcttcgg gataaccca gattcggcg gagtcgccg 
      tgcgtcaac acttactta agcaaaatg gggtcttca 
      tctggtaga gcatcaact cgcaccctg ttggaaatt 
      ctcatccat ttgatgcta gctctaagc gcattgtag
    mRNA sequence
    • >TC000981 RA
      ggtcgtcgt agaggcgtc gtcgtcgtg tccgcgatc 
      cgcagttgt gtgtgcgct cgaaaccga cacctgtta 
      gaagtggtg cgccggtcg gcgatggcg gccacgtga 
      accagccga agcagcatg ggttcgaat ctcgtcgtc 
      gtcgtggtc gtcgtggtg gtggtggtg gcgtcgctg 
      agtgcttcc gcccgcgcc tactcctgg cccagcgag 
      gagacgacg acgcgtccg ccccaagcc agagacttt 
      cacagcgac ccgctggtg gtggagacc accagcggc 
      ctggtgcgg ggcaaggcc aagacggtg ctgggccgc 
      gaggtgcac gtcttcacc gggattccc ttcgccaag 
      ccgcccatc gaacagctg aggttcagg aagcccgtg 
      cccatcgat ccctggcac ggcatctta gatgccacc 
      aagctgccc aactcgtgc taccaggag cgctacgag 
      tacttcccc ggctttgag ggcgaggag atgtggaac 
      cccaacacg aacatctcc gaggactgc ctctacctc 
      aacatctgg gtgccccag cgcttgcgc atccggcac 
      cacggcgag aagctcccc caggaccgg cccaaagtc 
      cccgtcctc gtgtggatc tacggcggg ggctacatg 
      agcggcacc tccaccctg gacatctac gacgccgac 
      atcatcgcc gccacctcc gacgtcatc gtcgcctcc 
      atgcaatat cgcgtgggg gcgttcggc ttcctctac 
      ttgagcaag tacttccct cgcggcagt gaggaggcg 
      cccggtaac atgggcatg tgggaccaa gccctggcc 
      atccgctgg atcaaggag aacgcggcg gcctttggg 
      ggcgaccca gacctcatc acgctgttt ggggagtcg 
      gctggaggc ggctccgtc agcatcctg ctcctgagt 
      ccggttact aaaggcctg gccaggagg gggattctg 
      cagtcgggg actatgaac gccccttgg agttacatg 
      tcgggggag agggcgcag cagatcggg aaggtcctg 
      gtggaggac tgcgggtgc aacgtctcc ttgttggag 
      acgaggccg catgaggtc attgattgc atgagggcg 
      gtggaggcc aagacgatt tcgctgcaa cagtggaat 
      tcgtattcg gggattttg ggcttcccc tcaacgcct 
      acggttgat ggcgtcttc atgcccaag catcccatg 
      gatatgctg gcggaaggg gattacgag gatatggag 
      atcctggtc gggagtaac caagatgaa ggcacttac 
      ttcttactt tacgatttt atcgatttc ttcgaaaag 
      gatggccct agcttcctc caacgagac aaataccac 
      gacattatc gatacgata ttcaaaaat atgagtcgg 
      ttggaacgt gatgccata gtatttcag tatactgat 
      tgggagcac gtcaacgac ggctacttg aaccagaaa 
      atggtgggc gacgtcgtc ggagattat tttttcatt 
      tgtccaacc aacgatttc gccgagctg gcagcagag 
      cgcggaatg aaagtctac tattatttt ttcacacac 
      aggacaagc acgtcgttg tggggcgaa tggatgggg 
      gtgatgcac ggggatgag atagaatac gtgtttggc 
      catcctttg aacatgtcg ttgcagttt aactcaagg 
      gaacgggaa ctcagtctg aagataatg caagccttt 
      gccagattt gcagcaacg gggaaacca gtgacagac 
      gacgtgaat tggccattg tacacaaaa gaccaaccg 
      cagtatttc atcttcaac gccgacaaa aacggcatc 
      ggcaaaggt cctcgagcg acagcgtgc gcgttttgg 
      aacgatttc ctgcccaag cttcgggat aacccagat 
      tcggcggag tcgccgtgc gtcaacact tacttaagc 
      aaaatgggg tcttcatct ggtagagca tcaactcgc 
      accctgttg gaaattctc atccatttg atgctagct 
      ctaagcgca ttgtagata agtatctct tatatgtaa 
      gcaaatgta ggcctactt agtcgcaaa gcttcagac 
      acagacgta gagaatacg aaatgaaat tagaataat 
      aatgtaaat tcgacaatc gctttctaa ttcgtcttg 
      tacatacgc attttggaa ctttcgaat cagaaagcg 
      atcgcaaga aatcaaaac ccggtctgg attaaacct 
      gaagcttgt gaatggagt cagtgtgta ccctggaaa 
      atcgacgtg atacaaagc gcctcattc tgccgatgt 
      caccgaatt gtgacactc aatgagaca cgagataga 
      aaaatgctt gttcttggg catgctcgt ggcaattgt 
      acgcattgt gtaacactt gactttgta taaactcca 
      atctgattg catttatac acagtcatc gacaatcat 
      cttaataat attaagaaa ccaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY