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Gene information
General information
TC000979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3646188..3652624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000979
    iBeetle screen iB_03112
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000979 RA
      MTPLAVVLT LFSCSVLVI RGHPGFEIQ PEVNARRLL 
      NSVHLQKQL TRGPLAQAR SKRTSDHII TDCIFMTSE 
      EGDFYHKSS IADGTACGA YIFSDPDQT IEVHFNYLD 
      VPCENGGLV SFVDGWELN GEFFPSPSD HPLPLNSRF 
      TEFCGKRKV KQTFKSSQN VALIQYRMP AKGTSFGFS 
      VRFIKNPTP CNVLFQSTE DIYTLRNYG KRSNCSLST 
      LFPAAVRVA SLNVGIVPS LGRGIELET GTIHKVRSQ 
      CQKRGLDDY LQIGGSRGL DNANLLLAD SVCGLDSKP 
      GKHVEVIAC GTTTVRLVS SGAFDNSAT VAIRGLTED 
      DINGYMSVF CQEEALE
    CDS sequence
    • >TC000979 RA
      atgaccccg ttggccgtc gttttgact ttgttttca 
      tgttcagta ctagtgatt agaggacat cccggattc 
      gaaatccag ccagaggtg aatgctaga cgtttgttg 
      aattctgtc catttacag aaacaatta actagagga 
      ccattagct caagcacgt tcgaaaaga acgtcagat 
      catataata acagattgc atttttatg acatctgaa 
      gaaggcgac ttttaccac aaatcgagc attgctgac 
      ggtaccgct tgtggtgct tacattttc agcgatccc 
      gaccaaacc atcgaagtc catttcaat tatttggac 
      gttccttgc gaaaacggc ggacttgtc tctttcgtg 
      gatggttgg gaactgaac ggcgaattt ttccccagc 
      ccctccgac caccccttg cccctaaat tcgcgattt 
      accgaattc tgcggcaag aggaaagtc aaacagacg 
      ttcaaaagc tcgcagaat gtggcactt atccagtat 
      cggatgccg gccaaaggc acaagtttc ggattcagc 
      gtcaggttt attaagaat ccgacgccc tgcaatgtg 
      ctgttccag tcaacggag gacatctac acgctgagg 
      aattacggc aagaggtcg aattgtagt ctgagcact 
      ttgtttcct gctgcagtc agggtcgct tcgctgaac 
      gttgggatt gtcccgagc cttggacgg ggcatcgag 
      ctggaaacc ggcaccatc cacaaggtg cggtcccag 
      tgtcagaaa cgcggcctg gacgactat ttgcaaatc 
      ggcggttcc cggggcttg gacaacgcg aatttgctc 
      ctagcggac tcagtttgt ggactggac tcgaaacca 
      ggaaagcac gtggaagtg atcgcatgt gggactacc 
      accgttcgt cttgtgtcc agtggagct ttcgataat 
      tccgcaaca gtggccatt cgaggactg acagaggac 
      gatatcaac ggatacatg agcgtcttt tgtcaagag 
      gaagcactc gaataa
    mRNA sequence
    • >TC000979 RA
      gagaaaaat accgctctt ttttgttaa acgatatgt 
      tcaattaaa tttaattgt gctgtgaat atgaatgca 
      cccgataat aaggtgtgt ttgttaatt aagttaatt 
      gcttattga gcaattttc agacgcgaa aagcctttt 
      tctctagtt tttatctcg agtgttaat tcgtgactt 
      taaatattc ttttgtcaa tgaaacgta gtgtaaaat 
      gttatgtaa attttaatt aaagacaac ggcgtaggg 
      taatgtaat gtaaattca attcattga tgctaaaag 
      tttaggcat ggagaaaaa cgtgccatt taaacggga 
      ttaaaattg gaaattgga tatttttga gcgcaaggg 
      ctgtttgga tgcttgtga gcgtagcga cgctattta 
      aacggaggg cgcgtcgtc cggcttcac tagtatcac 
      gacgtttgg gccgtatca ccaccacct gttaggatg 
      accccgttg gccgtcgtt ttgactttg ttttcatgt 
      tcagtacta gtgattaga ggacatccc ggattcgaa 
      atccagcca gaggtgaat gctagacgt ttgttgaat 
      tctgtccat ttacagaaa caattaact agaggacca 
      ttagctcaa gcacgttcg aaaagaacg tcagatcat 
      ataataaca gattgcatt tttatgaca tctgaagaa 
      ggcgacttt taccacaaa tcgagcatt gctgacggt 
      accgcttgt ggtgcttac attttcagc gatcccgac 
      caaaccatc gaagtccat ttcaattat ttggacgtt 
      ccttgcgaa aacggcgga cttgtctct ttcgtggat 
      ggttgggaa ctgaacggc gaatttttc cccagcccc 
      tccgaccac cccttgccc ctaaattcg cgatttacc 
      gaattctgc ggcaagagg aaagtcaaa cagacgttc 
      aaaagctcg cagaatgtg gcacttatc cagtatcgg 
      atgccggcc aaaggcaca agtttcgga ttcagcgtc 
      aggtttatt aagaatccg acgccctgc aatgtgctg 
      ttccagtca acggaggac atctacacg ctgaggaat 
      tacggcaag aggtcgaat tgtagtctg agcactttg 
      tttcctgct gcagtcagg gtcgcttcg ctgaacgtt 
      gggattgtc ccgagcctt ggacggggc atcgagctg 
      gaaaccggc accatccac aaggtgcgg tcccagtgt 
      cagaaacgc ggcctggac gactatttg caaatcggc 
      ggttcccgg ggcttggac aacgcgaat ttgctccta 
      gcggactca gtttgtgga ctggactcg aaaccagga 
      aagcacgtg gaagtgatc gcatgtggg actaccacc 
      gttcgtctt gtgtccagt ggagctttc gataattcc 
      gcaacagtg gccattcga ggactgaca gaggacgat 
      atcaacgga tacatgagc gtcttttgt caagaggaa 
      gcactcgaa taagactat taccaccac atatctgta 
      aatcaattt tttgtgtcg ctgtgtatt atatccgat 
      gtaattagt ttccggttc gatgaacaa ttatgtaat 
      ttaggtttt ttatgttag tggtaggtg ttccgtttg 
      tttcaataa aattaaata aaactgttt cttgtatta 
      aataaggag gtgttggtt gcgagcatt gtacccaca 
      tttcacgta caaggagaa tcaaataaa aacataaaa 
      aaatatctc attgcaatt aaatggcgt ttttagtta 
      cttaaacca ttaggaaat gtcttgagc gacagttta 
      tttattttt acttagttt agaagcctc aatgtatat 
      agtaatcta ataaattta tttaaacaa a