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Gene information
General information
TC000945 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8691520..8701288
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000945
    iBeetle screen iB_03107

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Larvae hatched from egg-shell. No phenotype scorable.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000945 RA
      MGWLRDLHT NGNKCIVID YKYNRVTLM GFASKGTEK 
      FIGCQTGGE GKGACPATN PPPKIPFER RQILAWAAS 
      QERSYVAGV SQTVHLEIG TVETRAGQH GPGGSLGLL 
      GDSRVANNI RGFFATRIG SALPDTVTT SRNPLSACW 
      SLAAMTTIP ILNMEWGEL KEVMWTKVQ EPRTQRKLI 
      LVIVSIALL LDNMLYMVI VPIIPDYLR YIGAYNDEV 
      TVPGNATLP PGTPYKHDH HGQDSATGI LFASKAIVQ 
      LMVNPFSGA LIDRIGYDI PMMIGLTIM FLSTAVFAC 
      GRSYGLLFF ARSLQGVGS AFADTSGLA MIADRFTEE 
      NERSKALGI ALAFISFGC LVAPPFGGA LYQFAGKEV 
      PFLILAFVS LMDGFMLLL VMKPLKTQL KEAGLAKPP 
      SVPIWRLFM DPYIAVCSG ALMMSNVAL AFLEPTISL 
      WMEDNLTTD NWKIGMIWL PAFFPHVFG VIFTVKMAK 
      KYPQYQWLM AAGGLALEG LCCFIIPFA SSYKVLMIP 
      ICGICFGVA LIDTALLPT LGYLVDVRY VSIYGSIYA 
      IADISYSVA YAIGPIIAG GVVEAIGFT ALNVGIAFS 
      NLLYAPVLM YLKHIYDFK PFENEANIL MSDPPNKEY 
      QTYSMQEQK PISDDIQNH LEYKQQETN FDSNGTYQQ 
      GYDQYQGYQ QSYQEVPAY QQPRPPQQA EQPSNPFRP 
      AETGRNPFR QGF
    CDS sequence
    • >TC000945 RA
      atggggtgg ttgcgagac ttgcataca aacgggaat 
      aaatgcatc gttatagat tataaatat aatcgggtt 
      actttaatg ggatttgca tcgaagggt acggagaaa 
      tttatcggc tgtcaaacc ggaggtgaa ggtaagggc 
      gcgtgcccc gccacaaac ccgcccccg aaaatcccg 
      ttcgaacgc cgccagatt ctagcgtgg gctgcgtct 
      caggagcgg tcgtacgtt gcgggagtg agccaaaca 
      gtgcacctg gagatcggc accgtggag actcgcgcc 
      ggccaacac ggcccggga ggcagcctt ggcctactc 
      ggagattcg cgcgtcgcc aacaacatt cgcggcttc 
      ttcgccacc cggatagga agcgccctt ccagacaca 
      gtaaccacg tcgcgcaat ccactatcc gcttgctgg 
      tctctggcc gccatgacg accatcccg atcctaaac 
      atggagtgg ggtgagctg aaggaagtg atgtggacc 
      aaagtgcaa gaaccgcgc acccaacgc aaactcatc 
      ctcgtgatc gtttccatc gccctcctc ctggacaac 
      atgctgtac atggtgatc gtgcccatc atccccgac 
      tatctgcga tacatcggt gcctacaac gacgaagtg 
      accgttccg ggaaacgcc acgcttcca ccaggtact 
      ccctacaag cacgaccat cacggccag gactcggct 
      accggcatc ctattcgca tccaaggcc atagtccag 
      ctgatggtc aatcctttc tcgggagct ctaatcgac 
      cgcatcggc tacgacatc ccaatgatg atcggactc 
      acgataatg tttctgtcg acggcagtg ttcgcctgt 
      ggccgcagc tacggcctc ctcttcttc gcccgaagc 
      ctccaaggg gtcggctcg gccttcgcc gacacctcg 
      gggctggcc atgatcgcc gaccgcttc acggaggaa 
      aacgaacgc tcgaaagcc ctcggcata gccctagct 
      ttcatcagt ttcgggtgt ctggtggcg ccgcctttc 
      ggaggagcc ttgtaccaa ttcgccgga aaggaagta 
      ccgttcctg atcctagcc ttcgtttca ctcatggac 
      ggctttatg ctgctcctg gtcatgaag cctttgaaa 
      acccaactc aaggaggca ggtctcgcc aagccgcct 
      tccgtgccc atctggcgc ctgttcatg gacccgtac 
      atcgcggtc tgttccggg gcgctgatg atgtcgaac 
      gtggcgttg gccttcctg gaacccacg atctccttg 
      tggatggaa gacaacctc accaccgac aactggaag 
      atcgggatg atttggctg cccgcgttc ttcccgcac 
      gtctttggc gtcatcttc acggtcaag atggccaag 
      aagtacccg cagtaccag tggctgatg gcggcaggg 
      ggtctcgct ttggaaggc ttgtgctgc ttcattatc 
      cccttcgcc agctcgtac aaagtcctc atgatcccg 
      atctgcggc atttgcttc ggagtcgct ttaatagac 
      accgctctc cttcccacc ctgggctac ctggtggac 
      gtcagatat gtttccatt tacggaagc atttacgca 
      atcgcagac atatcgtat tccgttgct tacgccatt 
      ggacctatc atcgctgga ggagtggtc gaagcgatc 
      ggtttcacg gcgcttaac gtaggtatc gccttttcc 
      aacttgctg tacgcgccg gtactcatg tacctcaaa 
      catatctac gacttcaaa ccattcgag aacgaagcc 
      aacatcctc atgtcggat cctccgaac aaggagtac 
      caaacgtac tccatgcag gagcaaaaa ccgatctcg 
      gatgacatt caaaaccat ctcgagtat aaacaacag 
      gagactaac ttcgattca aacgggacg taccagcaa 
      gggtacgac cagtaccaa ggctaccag cagagttac 
      caagaagta ccggcttac cagcaacct agaccgccc 
      caacaagcc gagcaacct tcgaacccg tttagacca 
      gccgaaaca ggacgaaat cccttcagg cagggtttc 
      taa
    mRNA sequence
    • >TC000945 RA
      tcggaggaa aaactttgg tgggcacac accctctcc 
      ggtacattt accggcgat cgaagtttc attcatagt 
      taattcata tttgagacg taacccaag ttggaaacc 
      aaggtcagt gctttacat taacggaag acacgagcg 
      taattcctt ttaacgcct gaacacaaa attaatttg 
      ctttagtca caactaatc tcaatatct cgcaaattc 
      ccataattt ttcatacgg gaatccccg aaaattggc 
      aaaatcttc gccaataaa tcagctaaa atcgttggg 
      tttttgcgg gaatgaaac cggtaagct gtttgaaag 
      ttgaacatt cgtcaagtg acctttttg tgaactttg 
      tggttgaaa cgatcgaag tttggaagg cggagttca 
      gcaggaaag ggcggatcc tgcaggagg cgtttaacg 
      ggtgtgact tggagcagg atcaatacc tggcctgct 
      gcaacttgc tatttttac aggcaggta gaacgactt 
      ctccccttg gtcaccaaa catcacttt caccttgag 
      ggtgctgtg atcgaaaat tcaaatgaa cagccccgg 
      gcttccgtc ccgaacagg caatggggt ggttgcgag 
      acttgcata caaacggga ataaatgca tcgttatag 
      attataaat ataatcggg ttactttaa tgggatttg 
      catcgaagg gtacggaga aatttatcg gctgtcaaa 
      ccggaggtg aaggtaagg gcgcgtgcc ccgccacaa 
      acccgcccc cgaaaatcc cgttcgaac gccgccaga 
      ttctagcgt gggctgcgt ctcaggagc ggtcgtacg 
      ttgcgggag tgagccaaa cagtgcacc tggagatcg 
      gcaccgtgg agactcgcg ccggccaac acggcccgg 
      gaggcagcc ttggcctac tcggagatt cgcgcgtcg 
      ccaacaaca ttcgcggct tcttcgcca cccggatag 
      gaagcgccc ttccagaca cagtaacca cgtcgcgca 
      atccactat ccgcttgct ggtctctgg ccgccatga 
      cgaccatcc cgatcctaa acatggagt ggggtgagc 
      tgaaggaag tgatgtgga ccaaagtgc aagaaccgc 
      gcacccaac gcaaactca tcctcgtga tcgtttcca 
      tcgccctcc tcctggaca acatgctgt acatggtga 
      tcgtgccca tcatccccg actatctgc gatacatcg 
      gtgcctaca acgacgaag tgaccgttc cgggaaacg 
      ccacgcttc caccaggta ctccctaca agcacgacc 
      atcacggcc aggactcgg ctaccggca tcctattcg 
      catccaagg ccatagtcc agctgatgg tcaatcctt 
      tctcgggag ctctaatcg accgcatcg gctacgaca 
      tcccaatga tgatcggac tcacgataa tgtttctgt 
      cgacggcag tgttcgcct gtggccgca gctacggcc 
      tcctcttct tcgcccgaa gcctccaag gggtcggct 
      cggccttcg ccgacacct cggggctgg ccatgatcg 
      ccgaccgct tcacggagg aaaacgaac gctcgaaag 
      ccctcggca tagccctag ctttcatca gtttcgggt 
      gtctggtgg cgccgcctt tcggaggag ccttgtacc 
      aattcgccg gaaaggaag taccgttcc tgatcctag 
      ccttcgttt cactcatgg acggcttta tgctgctcc 
      tggtcatga agcctttga aaacccaac tcaaggagg 
      caggtctcg ccaagccgc cttccgtgc ccatctggc 
      gcctgttca tggacccgt acatcgcgg tctgttccg 
      gggcgctga tgatgtcga acgtggcgt tggccttcc 
      tggaaccca cgatctcct tgtggatgg aagacaacc 
      tcaccaccg acaactgga agatcggga tgatttggc 
      tgcccgcgt tcttcccgc acgtctttg gcgtcatct 
      tcacggtca agatggcca agaagtacc cgcagtacc 
      agtggctga tggcggcag ggggtctcg ctttggaag 
      gcttgtgct gcttcatta tccccttcg ccagctcgt 
      acaaagtcc tcatgatcc cgatctgcg gcatttgct 
      tcggagtcg ctttaatag acaccgctc tccttccca 
      ccctgggct acctggtgg acgtcagat atgtttcca 
      tttacggaa gcatttacg caatcgcag acatatcgt 
      attccgttg cttacgcca ttggaccta tcatcgctg 
      gaggagtgg tcgaagcga tcggtttca cggcgctta 
      acgtaggta tcgcctttt ccaacttgc tgtacgcgc 
      cggtactca tgtacctca aacatatct acgacttca 
      aaccattcg agaacgaag ccaacatcc tcatgtcgg 
      atcctccga acaaggagt accaaacgt actccatgc 
      aggagcaaa aaccgatct cggatgaca ttcaaaacc 
      atctcgagt ataaacaac aggagacta acttcgatt 
      caaacggga cgtaccagc aagggtacg accagtacc 
      aaggctacc agcagagtt accaagaag taccggctt 
      accagcaac ctagaccgc cccaacaag ccgagcaac 
      cttcgaacc cgtttagac cagccgaaa caggacgaa 
      atcccttca ggcagggtt tctaagctc tatgtgtat 
      attgtatat tcgtgtaag tgtccctgg gtgttaatt 
      atatgtaca tatcacacc ggatgttcc tgaaacttg 
      tacatatcg gctgaaatt cataagcac actgtttaa 
      ccccactag acttagtct aagcgattt ttctagggg 
      attgagttg ttgttgcgt tttggtagg ttttggtag 
      agctagggt ttgagttga gttcagatt tcgttgtca 
      agtttttac ttatatcaa ctaagatca catacatat 
      cagaatcga atgattcgc ttgttatat cgtgatagg 
      tcgaaattc tggccactt aattatagc gtaggctag 
      ttttaaatt aagtcactg ataagtagc ccgcatcac 
      acccccgtc tcctccatt tatatatat attattcaa 
      gttggattt tattggtat agctctcga ctcgatagt 
      gtcaattcc cttgtgtat agtatttgt gcaaaatga 
      tcaaagtgt tattttgcc gttagtata aatacgttc 
      gggaaccag aaaatttgc tcagctatg tagatactc 
      aaaatatat gtatattta ccccaaaat ctttatcta 
      accctgttt taacggcaa ctatggctg cgacaagtt 
      aaatataat cgactgtga tgggcacgt cccgtgccc 
      ttaacaatt aaattggaa cttaacaat gagaactaa 
      taataaaat ccaataaac tataatgta tgtttataa 
      aataattag tacttggtt taaactgga actttctaa 
      aacatgctt gtagttgtc gtatctaag tatataaaa 
      ctataggaa agcaataaa aaaatagtt gaagccttt 
      cagttgacc aaatagcgc gaatttact tacctacat 
      cgaaacatt attaaatgt gtatctctg taaaggtgc 
      ctgtgacta tatttacta aagtagtac tttgagctt 
      gttttgtga aaaatgatc gttcaagtc gcgtaacgc 
      acttgaacg gattttgga ttagttacc gactgcagc 
      ttcttccat atccctcta gagtcagcg agggtcata 
      gataatgga ttctctcat tgacttggc tttaaatat 
      aatcttaga cttttgaat attttgaca ttttatctg 
      attgttttg taaagtact gatagttta aaatgttga 
      tcggaaagg atgcatgtg actagtaga atctagctc 
      tagtactaa tagtagctg tattgtttc aatgtaaag 
      tataatttc agaaaactg tataagggt gctgtttta 
      ctgttgtcg tgagcacta caggctcta actgattct 
      aaaaccaga tgattttat tttgaggaa agtctagta 
      ctagtattt agtaaattt gttatttct agaaccagt 
      tgcagcctt atatttatt ccaaggagc aatcacatg 
      tactgaccg tttatcgat atctgaatg ggtctatta 
      aactaattg ttattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY