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Gene information
General information
TC000926 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8576776..8589584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000926
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03106

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Hatched larvae stayed in a bend position (app. 30%).
    abdomen musculature random partiallyirregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    All larvae hatched from the egg-shell and look normal, no phenotype scorable.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000926 RA
      MMMMDVGMY GHHSQHGTY PSDNYYNYT SDIPNAQQM 
      PPHYSNYHY EDPYGYTGS EETPPSPQE VYYHHQTIP 
      QDNPIINTE TGLSYTNLD YGNSNFPPI NHQNYNEPY 
      QRTHENMLL RHHEEVADG HQHHFLHEN KYQLEVDSG 
      YHLGASSTP TNSCMEYQH LHRYKEEAL QGDGRMRQH 
      VMHNLSSVP QPQPVLPTY KWMQVKRNV PKPTVPKIP 
      PAEFPTTSS SSALESPNP ASRSSCLGS NTSSMLSLN 
      CLNTGRTNF TNKQLTELE KEFHFNKYL TRARRIEIA 
      SALQLNETQ VKIWFQNRR MKQKKRMKE GLIPPEPIS 
      ASLSTSNSN SPSSTNLIQ NEASNENSR ESN
    CDS sequence
    • >TC000926 RA
      atgatgatg atggacgtt gggatgtac ggccatcac 
      agtcaacat gggacatac ccatcggat aactactac 
      aactacacc agtgatata ccaaacgcc cagcaaatg 
      cccccacat tactccaat taccactac gaagaccca 
      tatgggtac accggctcg gaggaaaca ccaccgtcg 
      ccccaagaa gtctactac caccaccag accatcccc 
      caagacaat cccataatc aatacagag acaggacta 
      agttacaca aacttggat tatggtaac tcaaacttc 
      ccccccatc aaccaccag aattacaac gaaccctat 
      caaagaacc catgagaac atgctgctg aggcaccat 
      gaggaggtg gccgatggg caccagcac cacttcctc 
      cacgaaaac aagtaccag ttggaggtt gactccggg 
      taccacctt ggggcatcg agcaccccc acaaactcc 
      tgcatggag taccaacac ctccacagg tacaaggag 
      gaagcgctg cagggagat gggaggatg agacaacac 
      gttatgcat aatttgagt tcggttcct cagccacaa 
      ccagtgtta ccaacttat aagtggatg caagtcaaa 
      aggaatgta ccaaaacca acagtaccc aaaattccg 
      cccgccgag ttcccaacc accagcagc tcctcagct 
      ctagagagc cccaacccg gcatcacgc agctcctgc 
      ttgggctcg aacacctcc agcatgcta agtctcaac 
      tgtctcaac accggccga acaaacttc accaacaaa 
      caactgact gaactggag aaagagttc catttcaac 
      aagtaccta actcgggca cgacgaatc gaaatcgct 
      tcagctctc caactcaac gaaacacaa gtgaaaatc 
      tggttccag aatcggaga atgaagcag aagaaacgg 
      atgaaagaa ggactcata ccaccagaa ccgatctca 
      gctagttta agcacaagc aatagcaat tcgccaagt 
      tcaacaaac ttgattcaa aatgaagcc agcaacgaa 
      aacagccgt gaatcaaac tag
    mRNA sequence
    • >TC000926 RA
      tgtgacgtg tgtgcagtg ccgacttga tgaaaatga 
      tgatgatgg acgttggga tgtacggcc atcacagtc 
      aacatggga catacccat cggataact actacaact 
      acaccagtg atataccaa acgcccagc aaatgcccc 
      cacattact ccaattacc actacgaag acccatatg 
      ggtacaccg gctcggagg aaacaccac cgtcgcccc 
      aagaagtct actaccacc accagacca tcccccaag 
      acaatccca taatcaata cagagacag gactaagtt 
      acacaaact tggattatg gtaactcaa acttccccc 
      ccatcaacc accagaatt acaacgaac cctatcaaa 
      gaacccatg agaacatgc tgctgaggc accatgagg 
      aggtggccg atgggcacc agcaccact tcctccacg 
      aaaacaagt accagttgg aggttgact ccgggtacc 
      accttgggg catcgagca cccccacaa actcctgca 
      tggagtacc aacacctcc acaggtaca aggaggaag 
      cgctgcagg gagatggga ggatgagac aacacgtta 
      tgcataatt tgagttcgg ttcctcagc cacaaccag 
      tgttaccaa cttataagt ggatgcaag tcaaaagga 
      atgtaccaa aaccaacag tacccaaaa ttccgcccg 
      ccgagttcc caaccacca gcagctcct cagctctag 
      agagcccca acccggcat cacgcagct cctgcttgg 
      gctcgaaca cctccagca tgctaagtc tcaactgtc 
      tcaacaccg gccgaacaa acttcacca acaaacaac 
      tgactgaac tggagaaag agttccatt tcaacaagt 
      acctaactc gggcacgac gaatcgaaa tcgcttcag 
      ctctccaac tcaacgaaa cacaagtga aaatctggt 
      tccagaatc ggagaatga agcagaaga aacggatga 
      aagaaggac tcataccac cagaaccga tctcagcta 
      gtttaagca caagcaata gcaattcgc caagttcaa 
      caaacttga ttcaaaatg aagccagca acgaaaaca 
      gccgtgaat caaactagt gtgaaaaaa aacaactgt 
      gaaagttta tgtatcgcc aaagagata gagtagaca 
      ctcccgggc aaaaagttt ctttattta tttgggtac 
      tatatttat ttataattt atttaaaag tgtacaata 
      aagtactga actacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY