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Gene information
General information
TC000926 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8576776..8589584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000926
    iBeetle screen iB_03106

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Hatched larvae stayed in a bend position (app. 30%).
    abdomen musculature random partiallyirregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    All larvae hatched from the egg-shell and look normal, no phenotype scorable.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000926 RA
      MMMMDVGMY GHHSQHGTY PSDNYYNYT SDIPNAQQM 
PPHYSNYHY EDPYGYTGS EETPPSPQE VYYHHQTIP 
QDNPIINTE TGLSYTNLD YGNSNFPPI NHQNYNEPY 
QRTHENMLL RHHEEVADG HQHHFLHEN KYQLEVDSG 
YHLGASSTP TNSCMEYQH LHRYKEEAL QGDGRMRQH 
VMHNLSSVP QPQPVLPTY KWMQVKRNV PKPTVPKIP 
PAEFPTTSS SSALESPNP ASRSSCLGS NTSSMLSLN 
CLNTGRTNF TNKQLTELE KEFHFNKYL TRARRIEIA 
SALQLNETQ VKIWFQNRR MKQKKRMKE GLIPPEPIS 
ASLSTSNSN SPSSTNLIQ NEASNENSR ESN
    CDS sequence
    • >TC000926 RA
      atgatgatg atggacgtt gggatgtac ggccatcac 
agtcaacat gggacatac ccatcggat aactactac 
aactacacc agtgatata ccaaacgcc cagcaaatg 
cccccacat tactccaat taccactac gaagaccca 
tatgggtac accggctcg gaggaaaca ccaccgtcg 
ccccaagaa gtctactac caccaccag accatcccc 
caagacaat cccataatc aatacagag acaggacta 
agttacaca aacttggat tatggtaac tcaaacttc 
ccccccatc aaccaccag aattacaac gaaccctat 
caaagaacc catgagaac atgctgctg aggcaccat 
gaggaggtg gccgatggg caccagcac cacttcctc 
cacgaaaac aagtaccag ttggaggtt gactccggg 
taccacctt ggggcatcg agcaccccc acaaactcc 
tgcatggag taccaacac ctccacagg tacaaggag 
gaagcgctg cagggagat gggaggatg agacaacac 
gttatgcat aatttgagt tcggttcct cagccacaa 
ccagtgtta ccaacttat aagtggatg caagtcaaa 
aggaatgta ccaaaacca acagtaccc aaaattccg 
cccgccgag ttcccaacc accagcagc tcctcagct 
ctagagagc cccaacccg gcatcacgc agctcctgc 
ttgggctcg aacacctcc agcatgcta agtctcaac 
tgtctcaac accggccga acaaacttc accaacaaa 
caactgact gaactggag aaagagttc catttcaac 
aagtaccta actcgggca cgacgaatc gaaatcgct 
tcagctctc caactcaac gaaacacaa gtgaaaatc 
tggttccag aatcggaga atgaagcag aagaaacgg 
atgaaagaa ggactcata ccaccagaa ccgatctca 
gctagttta agcacaagc aatagcaat tcgccaagt 
tcaacaaac ttgattcaa aatgaagcc agcaacgaa 
aacagccgt gaatcaaac tag
    mRNA sequence
    • >TC000926 RA
      tgtgacgtg tgtgcagtg ccgacttga tgaaaatga 
tgatgatgg acgttggga tgtacggcc atcacagtc 
aacatggga catacccat cggataact actacaact 
acaccagtg atataccaa acgcccagc aaatgcccc 
cacattact ccaattacc actacgaag acccatatg 
ggtacaccg gctcggagg aaacaccac cgtcgcccc 
aagaagtct actaccacc accagacca tcccccaag 
acaatccca taatcaata cagagacag gactaagtt 
acacaaact tggattatg gtaactcaa acttccccc 
ccatcaacc accagaatt acaacgaac cctatcaaa 
gaacccatg agaacatgc tgctgaggc accatgagg 
aggtggccg atgggcacc agcaccact tcctccacg 
aaaacaagt accagttgg aggttgact ccgggtacc 
accttgggg catcgagca cccccacaa actcctgca 
tggagtacc aacacctcc acaggtaca aggaggaag 
cgctgcagg gagatggga ggatgagac aacacgtta 
tgcataatt tgagttcgg ttcctcagc cacaaccag 
tgttaccaa cttataagt ggatgcaag tcaaaagga 
atgtaccaa aaccaacag tacccaaaa ttccgcccg 
ccgagttcc caaccacca gcagctcct cagctctag 
agagcccca acccggcat cacgcagct cctgcttgg 
gctcgaaca cctccagca tgctaagtc tcaactgtc 
tcaacaccg gccgaacaa acttcacca acaaacaac 
tgactgaac tggagaaag agttccatt tcaacaagt 
acctaactc gggcacgac gaatcgaaa tcgcttcag 
ctctccaac tcaacgaaa cacaagtga aaatctggt 
tccagaatc ggagaatga agcagaaga aacggatga 
aagaaggac tcataccac cagaaccga tctcagcta 
gtttaagca caagcaata gcaattcgc caagttcaa 
caaacttga ttcaaaatg aagccagca acgaaaaca 
gccgtgaat caaactagt gtgaaaaaa aacaactgt 
gaaagttta tgtatcgcc aaagagata gagtagaca 
ctcccgggc aaaaagttt ctttattta tttgggtac 
tatatttat ttataattt atttaaaag tgtacaata 
aagtactga actacat
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