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Gene information
General information
TC000925 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3035523..3056581
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000925
    iBeetle screen iB_03105
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    maxilla potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Distal part of the maxialla potentially transformed to leg.
    labium potentially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Diistal part of the labium potentially transformed to leg.
    legs number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000925 RA
      MRVKHPLRM PTGHLDDDD FVVVKTRSN SPEAGGGGF 
      WLAAASGAP HPAALPIEG CNETGFINS QPSMAEFMT 
      ALPHLSGDM THGASISPQ NTPPAGYPM DVPHGMGSP 
      GVNVPEYPW MKEKKTTRK SSQQENGLP RRLRTAYTN 
      TQLLELEKE FHFNKYLCR PRRIEIAAS LDLTERQVK 
      VWFQNRRMK HKRQTLGKQ GDDGDDKDS ITSDGGKNG 
      KLSDKFLDD EMSKKSCQG CEMPSAGLC GSHDEIPDI 
      SSTRGNNNN TPSATNNNT NFNNNSNGA SSVGSSSPF 
      DKLIEEDSR SNEDTSLHP SPRITKKAN SVSVKVENR 
      RNSPNSCEK KIGLSKISP KESQDAANV KLSPKSASS 
      LPPGNNIHG MAPSVMYPH LNRSSPTTA TAIASATVT 
      IQNVSNTIP PFASRGSMH FPNQYQMNQ EYRTDRKHT 
      QQHYQMNQN MYPGEMYNP EHTTVNEAQ PYVRNTTVP 
      TQGVARMSR GRQYNYPNP QQQYYYNKN HPNSENYIN 
      HQNYVQGYQ GEHPNYNHY GYHYSGDGG EVAHLPNTH 
      MGHEPGAAY YQNENMLQK NAEYSGKVG YYENNHYNG 
      DPNYISPEV FPNTNNAIM TPPASVQTE SSDNYNSFH 
      QFYSSESSQ NQVAPTGEN SNSSSDFNF LSNLANDYT 
      PEYYQI
    CDS sequence
    • >TC000925 RA
      atgagagtc aaacatccg ctccggatg ccgacggga 
      cacttagac gacgacgac ttcgtggtg gtcaagacg 
      aggtccaac agccccgag gccgggggc ggcggcttc 
      tggctggcg gcggcgtcg ggggcgccg caccccgcg 
      gctttgccc atcgaggga tgcaacgag accggcttc 
      attaacagc cagccatcg atggccgag ttcatgacg 
      gccctgccg catctttcc ggggacatg acccacggc 
      gcctccatc agcccgcag aacacgccg ccggcgggc 
      taccccatg gacgtgccg cacgggatg gggtccccc 
      ggagtcaac gtgccggag tacccctgg atgaaggag 
      aagaagacc acgagaaag agtagccag caagaaaat 
      ggacttcca cgaagatta aggacagca tataccaac 
      acccaactg ttagaatta gaaaaggag tttcacttt 
      aataaatat ctatgtcgt cctagaaga atagaaata 
      gctgcttcg ctagacctt accgagcgt caggtaaaa 
      gtctggttt caaaaccgg cgcatgaaa cacaagcgg 
      caaaccctc ggcaagcag ggcgacgac ggcgacgac 
      aaagactcg atcaccagc gacggtggc aaaaacggc 
      aaactctcc gacaagttc ctggacgac gaaatgtcc 
      aagaagagt tgccaaggc tgcgaaatg ccttcggcg 
      ggcctgtgc ggctcccac gacgagatc cccgacatc 
      agttcaacc cgaggcaac aacaacaac accccaagt 
      gcaacaaac aacaacacg aacttcaac aacaacagc 
      aacggagcg agcagcgtc ggaagctcc agccctttc 
      gacaaactc atcgaagag gactcccga tcgaacgaa 
      gacactagc ctacaccca tcacccagg ataacaaaa 
      aaggcaaac tcggttagc gtcaaggta gagaaccgc 
      aggaattcg cccaactcc tgcgagaag aaaatcgga 
      ctgagcaag atttcgccg aaggaaagt caagacgcc 
      gccaacgtg aaactgtcg ccgaaaagt gcgtcctcc 
      ctaccccca ggaaacaac atccacggg atggcccca 
      agcgtcatg tacccacat ttgaaccgc tcctcgccc 
      acgacagca acagcaatc gcctcagcg accgtcacc 
      atccaaaat gttagcaac acgatcccg cctttcgcc 
      tcccgaggc tcaatgcat tttcccaac cagtaccaa 
      atgaaccag gagtaccga accgaccgg aaacacacc 
      cagcagcac taccaaatg aaccagaac atgtaccct 
      ggcgagatg tacaacccg gagcacacg acagtcaac 
      gaggcccag ccctacgtc cggaacaca acggtacca 
      acgcaaggg gtggcaagg atgtcccgc ggccgccag 
      tacaactac cccaacccc caacagcag tattactac 
      aacaagaac caccccaat tccgagaac tacatcaac 
      caccagaac tacgtgcag ggctaccag ggcgagcac 
      ccgaactac aaccactat gggtaccac tacagcggc 
      gacgggggc gaggtggcg cacttgccc aacacccat 
      atgggccac gagccgggc gcggcctac taccagaac 
      gagaacatg ctgcagaag aacgccgag tattcggga 
      aaagtcggc tattatgag aacaaccac tacaatggt 
      gatcctaat tacatttcc ccggaagtt ttccccaat 
      acgaacaat gcgataatg accccacct gcgagtgta 
      caaactgaa tctagtgat aattacaat tcgtttcat 
      caattctat tccagtgag tcttcacaa aatcaagtc 
      gcaccgacc ggtgagaac agtaacagt tcgtcggac 
      tttaatttt ttgagtaat ctggcaaac gactatacg 
      cccgagtac taccagatt tga
    mRNA sequence
    • >TC000925 RA
      ggcgctcca cggcaccac gtgaccggc catcatcct 
      cctcaatga gaaagaaac aaaaacaac cgtcactaa 
      cagtaactg tgaacacac acgtaacaa tcgtttcct 
      atttccagc tggctccgc ttgaatttc gcgggtttt 
      gtgactagt gagtggacc tagtgattg tgatggttc 
      ctagtgact ccggtcaat gagagtcaa acatccgct 
      ccggatgcc gacgggaca cttagacga cgacgactt 
      cgtggtggt caagacgag gtccaacag ccccgaggc 
      cgggggcgg cggcttctg gctggcggc ggcgtcggg 
      ggcgccgca ccccgcggc tttgcccat cgagggatg 
      caacgagac cggcttcat taacagcca gccatcgat 
      ggccgagtt catgacggc cctgccgca tctttccgg 
      ggacatgac ccacggcgc ctccatcag cccgcagaa 
      cacgccgcc ggcgggcta ccccatgga cgtgccgca 
      cgggatggg gtcccccgg agtcaacgt gccggagta 
      cccctggat gaaggagaa gaagaccac gagaaagag 
      tagccagca agaaaatgg acttccacg aagattaag 
      gacagcata taccaacac ccaactgtt agaattaga 
      aaaggagtt tcactttaa taaatatct atgtcgtcc 
      tagaagaat agaaatagc tgcttcgct agaccttac 
      cgagcgtca ggtaaaagt ctggtttca aaaccggcg 
      catgaaaca caagcggca aaccctcgg caagcaggg 
      cgacgacgg cgacgacaa agactcgat caccagcga 
      cggtggcaa aaacggcaa actctccga caagttcct 
      ggacgacga aatgtccaa gaagagttg ccaaggctg 
      cgaaatgcc ttcggcggg cctgtgcgg ctcccacga 
      cgagatccc cgacatcag ttcaacccg aggcaacaa 
      caacaacac cccaagtgc aacaaacaa caacacgaa 
      cttcaacaa caacagcaa cggagcgag cagcgtcgg 
      aagctccag ccctttcga caaactcat cgaagagga 
      ctcccgatc gaacgaaga cactagcct acacccatc 
      acccaggat aacaaaaaa ggcaaactc ggttagcgt 
      caaggtaga gaaccgcag gaattcgcc caactcctg 
      cgagaagaa aatcggact gagcaagat ttcgccgaa 
      ggaaagtca agacgccgc caacgtgaa actgtcgcc 
      gaaaagtgc gtcctccct acccccagg aaacaacat 
      ccacgggat ggccccaag cgtcatgta cccacattt 
      gaaccgctc ctcgcccac gacagcaac agcaatcgc 
      ctcagcgac cgtcaccat ccaaaatgt tagcaacac 
      gatcccgcc tttcgcctc ccgaggctc aatgcattt 
      tcccaacca gtaccaaat gaaccagga gtaccgaac 
      cgaccggaa acacaccca gcagcacta ccaaatgaa 
      ccagaacat gtaccctgg cgagatgta caacccgga 
      gcacacgac agtcaacga ggcccagcc ctacgtccg 
      gaacacaac ggtaccaac gcaaggggt ggcaaggat 
      gtcccgcgg ccgccagta caactaccc caaccccca 
      acagcagta ttactacaa caagaacca ccccaattc 
      cgagaacta catcaacca ccagaacta cgtgcaggg 
      ctaccaggg cgagcaccc gaactacaa ccactatgg 
      gtaccacta cagcggcga cgggggcga ggtggcgca 
      cttgcccaa cacccatat gggccacga gccgggcgc 
      ggcctacta ccagaacga gaacatgct gcagaagaa 
      cgccgagta ttcgggaaa agtcggcta ttatgagaa 
      caaccacta caatggtga tcctaatta catttcccc 
      ggaagtttt ccccaatac gaacaatgc gataatgac 
      cccacctgc gagtgtaca aactgaatc tagtgataa 
      ttacaattc gtttcatca attctattc cagtgagtc 
      ttcacaaaa tcaagtcgc accgaccgg tgagaacag 
      taacagttc gtcggactt taatttttt gagtaatct 
      ggcaaacga ctatacgcc cgagtacta ccagatttg 
      attcagtgc aatggacag ttctgtctt ctgttaatt 
      tttactgtt actgatgtg atttttatt gtttttggt 
      ttttcgatt ctagtattg ttcacgttg aatgtatac 
      actttataa aaacgcttc tagccaaca tttagacaa 
      tctaaaagt tgtgtttgt tattgatac aatcagaaa 
      acaatcatt tttgtttca tcgcacgat cccgccgga 
      gtctcatta ttaacataa atgtatgta ctattttag 
      gacaaacct gtaggttgt tgttgaatg taaatactt 
      tgcaattta atgtaatac taatattgt acagttaga 
      gacacattg gtacacttt agcatttta attcggaaa 
      atatacgac tgtagcttt a