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Gene information
General information
TC000912 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8314775..8339431
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000912
    iBeetle screen iB_03102

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 thorax musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax musculature dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature dorsal partiallynot fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    prothorax decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    prothoracic leg shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Complete leg reduced to stumps.
    legs femur decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs potentiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Coxa seems unaffected. Trochanter, femur, tibiatarsus, pretarsus mostly not present, potentially transformed to antenna.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000912 RA
      MSSYFANSY MPDMRNGGV VSAEHPHQH QHYGAAVQV 
      PQGGGAVQP DPGSCDPSV GLRQGIPPH HYGGPPSGG 
      QPPQGMPYP RFPPYDRMD IRAAGYYGP QQQMDGQEY 
      RPDSPSSMH MANTAAPNG HQTQVVYAS CKLQAAAVT 
      QNGVLGPTG SPPLTTQSM NNHHMGHHM QEQHPQHHQ 
      PHHQQQHMM YGGQQGANM HQQGPPHQQ PPIQQQPNQ 
      GQQPPGNTA AALPSPLYP WMRSQFERK RGRQTYTRY 
      QTLELEKEF HFNRYLTRR RRIEIAHAL CLTERQIKI 
      WFQNRRMKW KKENKTKGE GGSEGGGDD ISPQGSPQ
    CDS sequence
    • >TC000912 RA
      atgagttca tatttcgcc aattcctat atgccggac 
      atgcgaaat gggggtgta gtttccgcg gagcatccg 
      caccagcac cagcactac ggcgccgcc gtccaggtg 
      ccccagggg ggcggcgcc gtgcagccc gacccgggg 
      tcgtgcgac ccctcggtg gggctgcgc cagggcatc 
      cctccgcac cactatggg gggcccccg tcgggcggg 
      cagccgccc cagggcatg ccctacccc cgcttcccg 
      ccgtacgac cggatggac atccgcgcc gccggctac 
      tacggcccc cagcagcag atggacggc caggagtac 
      cgccccgac agccccagc agcatgcac atggccaac 
      accgccgcc cccaacggc caccaaacg caggtggtc 
      tacgccagc tgtaagctg caagccgcc gccgtcacg 
      cagaacggg gtgctgggg cccaccggg agtccgccc 
      ctgaccacc cagtcgatg aacaaccat cacatggga 
      caccacatg caggagcag cacccccag caccaccag 
      ccccaccac cagcagcag cacatgatg tacgggggg 
      cagcaaggc gccaacatg caccagcaa ggacccccg 
      caccagcag ccccccata cagcaacag cccaaccaa 
      gggcagcag cccccgggg aacacggcg gcggctctg 
      cccagcccc ctgtacccc tggatgagg agccaattt 
      gagcggaag cgcggccgt cagacgtac acccgctac 
      cagacgtta gaactggag aaagagttc catttcaac 
      cggtatttg acgagacgg cgacgcatc gagatcgcc 
      cacgcgctg tgtttaacc gaaagacag atcaagata 
      tggtttcaa aaccggcgt atgaaatgg aaaaaggag 
      aacaagacc aaaggcgaa ggcggctcg gaaggcggt 
      ggagacgac atcagcccc cagggctcc cctcagtga
    mRNA sequence
    • >TC000912 RA
      caggaggtg tgcgtgcgc tcggcatat agtgatgtt 
      tgaaacgct gacagcttg ccagtacta ttacaagct 
      cgtccacca atcccagtg caaactggc tgtgcccct 
      ctcgatttt gacagatcc acgcgatta tgaatcacc 
      aagtttgac agttgagtg cgcgacgcg gtgcagcat 
      cgtcttggt ggaagcgcg cgcgatgtg tggacgtcg 
      tcgtcgtca gcgtccagg aggtggtgc ggcggcttg 
      tgtggtccg cggtaattg acatgttaa gcttcgcca 
      taacgcgat ttaaaacgg accggctgc aagcctcga 
      tggggactc gaataatgc cgaaacagg tttacgaat 
      tttccgcgt gaagttatt atcgtttaa tcgtgtcgt 
      cgccgtctt cggagattc ccaatcagg tagagtcct 
      ttaatgaca attcttgta aaacaaaac gctgcttaa 
      tgagaacgt acggataaa tactgaccg cacgctaaa 
      tccatccga agccaatta ttcaatata attaccaca 
      ctttgttgc tattaatcc aattaacta ttaataaac 
      caaatcgtt gaagcttga atttgagta gaatgcggt 
      cgaatattt tctctcggg tcgttagcg agtcttgtt 
      tttagaaac gacaatttt ttcggttgt tttctcatc 
      cgttacgtt ttgttactt cttggaata aatatctag 
      atcttgttt ggaacgact tggcctttg acacgaccg 
      cattaaaac aagactcac ccgaaaaca tttatacac 
      ctagtgtag tagggctgt atatgtcac gtagttgag 
      gtttctttt ccaaccact taacgactt tgatgaatt 
      gcttagcag gaggaaagt cgagaaagc tggaaacgg 
      ccaaatatg gcaggaaaa atgccggtt caagggtta 
      aaagccgag ttaaggaat tcccgcttg ttggctttg 
      ttattgtct ttgggtttt cgaaaattc ttgagttag 
      ttaactagt tgcaagtgt cgcattccg cggccacgt 
      gtttacatg aagcgtgga acccaaatg atgatggtt 
      tcagtttcc atatgtcag tcatttcag ttccttaga 
      gaaaatatt tagtttgcc atttgaact tgagtgcaa 
      atcattgac tggcgtctt gttgcaggg ccctatgcc 
      gatgaggaa cgccgtcgc cgccacgtc ccccaggtc 
      cctcttgtg aaccctagt gtacagtga ccgtggcga 
      tgacaaaat gtctttcta gtgcatagt gctctctta 
      aaactgtgt ctctgtgaa gaattatga gttcatatt 
      tcgccaatt cctatatgc cggacatgc gaaatgggg 
      gtgtagttt ccgcggagc atccgcacc agcaccagc 
      actacggcg ccgccgtcc aggtgcccc aggggggcg 
      gcgccgtgc agcccgacc cggggtcgt gcgacccct 
      cggtggggc tgcgccagg gcatccctc cgcaccact 
      atggggggc ccccgtcgg gcgggcagc cgccccagg 
      gcatgccct acccccgct tcccgccgt acgaccgga 
      tggacatcc gcgccgccg gctactacg gcccccagc 
      agcagatgg acggccagg agtaccgcc ccgacagcc 
      ccagcagca tgcacatgg ccaacaccg ccgccccca 
      acggccacc aaacgcagg tggtctacg ccagctgta 
      agctgcaag ccgccgccg tcacgcaga acggggtgc 
      tggggccca ccgggagtc cgcccctga ccacccagt 
      cgatgaaca accatcaca tgggacacc acatgcagg 
      agcagcacc cccagcacc accagcccc accaccagc 
      agcagcaca tgatgtacg gggggcagc aaggcgcca 
      acatgcacc agcaaggac ccccgcacc agcagcccc 
      ccatacagc aacagccca accaagggc agcagcccc 
      cggggaaca cggcggcgg ctctgccca gccccctgt 
      acccctgga tgaggagcc aatttgagc ggaagcgcg 
      gccgtcaga cgtacaccc gctaccaga cgttagaac 
      tggagaaag agttccatt tcaaccggt atttgacga 
      gacggcgac gcatcgaga tcgcccacg cgctgtgtt 
      taaccgaaa gacagatca agatatggt ttcaaaacc 
      ggcgtatga aatggaaaa aggagaaca agaccaaag 
      gcgaaggcg gctcggaag gcggtggag acgacatca 
      gcccccagg gctcccctc agtgaactc ctcgtcacg 
      ataaccatg tacctaatt acacattgt agtcattgc 
      attttcttt tgccgggat ctccgacga gaaggtgcg 
      ctctttcgg caggaactc tccaaattc cccaaccac 
      aatgacctc ttcaacaag tcctctgtg cataagtcg 
      atagaattt agttccgat gaacgcgac tcatcgaat 
      ttatagttt tatttattt atatattgt atagatagt 
      agatattat gcgcgaatt tatatttga tggacacta 
      cacggattc atttagagg ccagtgcta cgtgcaatc 
      agtgatgca gacgaactg cccagtgat taaaaataa 
      aattttttg tataaatat tttctataa tgttaaaaa 
      cgagtggtc gctacggtt atgaagctc cactgcaga 
      ttttcaggt cagtgaatg agttgtttt ttttcaaag 
      ctgacgaag cgttttttt acaatgttt ccagtgagt 
      gtgaactct ttagacacc tgagatagc tacgaaagt 
      gtcgttcgg catagaatt gtttactta aagtgtaga 
      tatttcgaa tgaatgtat atttggttt taatactaa 
      taatgatgt aaataaatc cggtaacaa aagaaaagg 
      atatgaaat atacgatat tattaaaat tacgcactt 
      tcatgccaa aggtagagt aacgttatc catgtagga 
      cgttaacaa tactcaaac actttaatt agtaggaat 
      ttgacgcat tcggagtat ccaactttt ttttaacct 
      gtttttttc tgaaaaaaa gagacaaat attttatat 
      accatttaa ttggccgca attggagac gaacagtct 
      ttaaaattg ctagtgata acagtaacg aaatacgta 
      acgctgcta caaatcgag tatacgtaa ctaattatt 
      ttaatataa aaatcagag ctatgtaag aacggttag 
      gcaaattaa ataggtttc aaaattgag ttattgaga 
      gtcgaggtt ccagagccc ctatttaag ttggttaac 
      catttgtat acacaacta tttatacta ctataaatt 
      aggatttgc agtggcatg ctgtatatt tgtacctat 
      atggataaa ttagagaat agttctttg tgctgtggt 
      gtaatattt tagattaga agtactaag tttggccga 
      cacttaatt tcggacaaa ttaccattt tgtaaatga 
      acaactgta acataaatt tatcgttta accatcccg 
      cataaaact ttattttga tttaaaaac caacgaaac 
      tttaagtac tttaatact tggatttct gtcttgtgg 
      atttcaact tatatatac cgtacatat aattaatat 
      atgtataaa taaaatatt attgtaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY