General information | |||
TC000912 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 8314775..8339431 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000912 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03102 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)thorax musculature dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature dorsal partiallynot fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
prothorax decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
prothoracic leg shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
Comment: Complete leg reduced to stumps. |
legs shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs tibiotarsus decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs potentiallytransformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: Coxa seems unaffected. Trochanter, femur, tibiatarsus, pretarsus mostly not present, potentially transformed to antenna. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000912 RA
MSSYFANSY MPDMRNGGV VSAEHPHQH QHYGAAVQV PQGGGAVQP DPGSCDPSV GLRQGIPPH HYGGPPSGG QPPQGMPYP RFPPYDRMD IRAAGYYGP QQQMDGQEY RPDSPSSMH MANTAAPNG HQTQVVYAS CKLQAAAVT QNGVLGPTG SPPLTTQSM NNHHMGHHM QEQHPQHHQ PHHQQQHMM YGGQQGANM HQQGPPHQQ PPIQQQPNQ GQQPPGNTA AALPSPLYP WMRSQFERK RGRQTYTRY QTLELEKEF HFNRYLTRR RRIEIAHAL CLTERQIKI WFQNRRMKW KKENKTKGE GGSEGGGDD ISPQGSPQ
- >TC000912 RA
atgagttca tatttcgcc aattcctat atgccggac atgcgaaat gggggtgta gtttccgcg gagcatccg caccagcac cagcactac ggcgccgcc gtccaggtg ccccagggg ggcggcgcc gtgcagccc gacccgggg tcgtgcgac ccctcggtg gggctgcgc cagggcatc cctccgcac cactatggg gggcccccg tcgggcggg cagccgccc cagggcatg ccctacccc cgcttcccg ccgtacgac cggatggac atccgcgcc gccggctac tacggcccc cagcagcag atggacggc caggagtac cgccccgac agccccagc agcatgcac atggccaac accgccgcc cccaacggc caccaaacg caggtggtc tacgccagc tgtaagctg caagccgcc gccgtcacg cagaacggg gtgctgggg cccaccggg agtccgccc ctgaccacc cagtcgatg aacaaccat cacatggga caccacatg caggagcag cacccccag caccaccag ccccaccac cagcagcag cacatgatg tacgggggg cagcaaggc gccaacatg caccagcaa ggacccccg caccagcag ccccccata cagcaacag cccaaccaa gggcagcag cccccgggg aacacggcg gcggctctg cccagcccc ctgtacccc tggatgagg agccaattt gagcggaag cgcggccgt cagacgtac acccgctac cagacgtta gaactggag aaagagttc catttcaac cggtatttg acgagacgg cgacgcatc gagatcgcc cacgcgctg tgtttaacc gaaagacag atcaagata tggtttcaa aaccggcgt atgaaatgg aaaaaggag aacaagacc aaaggcgaa ggcggctcg gaaggcggt ggagacgac atcagcccc cagggctcc cctcagtga
- >TC000912 RA
caggaggtg tgcgtgcgc tcggcatat agtgatgtt tgaaacgct gacagcttg ccagtacta ttacaagct cgtccacca atcccagtg caaactggc tgtgcccct ctcgatttt gacagatcc acgcgatta tgaatcacc aagtttgac agttgagtg cgcgacgcg gtgcagcat cgtcttggt ggaagcgcg cgcgatgtg tggacgtcg tcgtcgtca gcgtccagg aggtggtgc ggcggcttg tgtggtccg cggtaattg acatgttaa gcttcgcca taacgcgat ttaaaacgg accggctgc aagcctcga tggggactc gaataatgc cgaaacagg tttacgaat tttccgcgt gaagttatt atcgtttaa tcgtgtcgt cgccgtctt cggagattc ccaatcagg tagagtcct ttaatgaca attcttgta aaacaaaac gctgcttaa tgagaacgt acggataaa tactgaccg cacgctaaa tccatccga agccaatta ttcaatata attaccaca ctttgttgc tattaatcc aattaacta ttaataaac caaatcgtt gaagcttga atttgagta gaatgcggt cgaatattt tctctcggg tcgttagcg agtcttgtt tttagaaac gacaatttt ttcggttgt tttctcatc cgttacgtt ttgttactt cttggaata aatatctag atcttgttt ggaacgact tggcctttg acacgaccg cattaaaac aagactcac ccgaaaaca tttatacac ctagtgtag tagggctgt atatgtcac gtagttgag gtttctttt ccaaccact taacgactt tgatgaatt gcttagcag gaggaaagt cgagaaagc tggaaacgg ccaaatatg gcaggaaaa atgccggtt caagggtta aaagccgag ttaaggaat tcccgcttg ttggctttg ttattgtct ttgggtttt cgaaaattc ttgagttag ttaactagt tgcaagtgt cgcattccg cggccacgt gtttacatg aagcgtgga acccaaatg atgatggtt tcagtttcc atatgtcag tcatttcag ttccttaga gaaaatatt tagtttgcc atttgaact tgagtgcaa atcattgac tggcgtctt gttgcaggg ccctatgcc gatgaggaa cgccgtcgc cgccacgtc ccccaggtc cctcttgtg aaccctagt gtacagtga ccgtggcga tgacaaaat gtctttcta gtgcatagt gctctctta aaactgtgt ctctgtgaa gaattatga gttcatatt tcgccaatt cctatatgc cggacatgc gaaatgggg gtgtagttt ccgcggagc atccgcacc agcaccagc actacggcg ccgccgtcc aggtgcccc aggggggcg gcgccgtgc agcccgacc cggggtcgt gcgacccct cggtggggc tgcgccagg gcatccctc cgcaccact atggggggc ccccgtcgg gcgggcagc cgccccagg gcatgccct acccccgct tcccgccgt acgaccgga tggacatcc gcgccgccg gctactacg gcccccagc agcagatgg acggccagg agtaccgcc ccgacagcc ccagcagca tgcacatgg ccaacaccg ccgccccca acggccacc aaacgcagg tggtctacg ccagctgta agctgcaag ccgccgccg tcacgcaga acggggtgc tggggccca ccgggagtc cgcccctga ccacccagt cgatgaaca accatcaca tgggacacc acatgcagg agcagcacc cccagcacc accagcccc accaccagc agcagcaca tgatgtacg gggggcagc aaggcgcca acatgcacc agcaaggac ccccgcacc agcagcccc ccatacagc aacagccca accaagggc agcagcccc cggggaaca cggcggcgg ctctgccca gccccctgt acccctgga tgaggagcc aatttgagc ggaagcgcg gccgtcaga cgtacaccc gctaccaga cgttagaac tggagaaag agttccatt tcaaccggt atttgacga gacggcgac gcatcgaga tcgcccacg cgctgtgtt taaccgaaa gacagatca agatatggt ttcaaaacc ggcgtatga aatggaaaa aggagaaca agaccaaag gcgaaggcg gctcggaag gcggtggag acgacatca gcccccagg gctcccctc agtgaactc ctcgtcacg ataaccatg tacctaatt acacattgt agtcattgc attttcttt tgccgggat ctccgacga gaaggtgcg ctctttcgg caggaactc tccaaattc cccaaccac aatgacctc ttcaacaag tcctctgtg cataagtcg atagaattt agttccgat gaacgcgac tcatcgaat ttatagttt tatttattt atatattgt atagatagt agatattat gcgcgaatt tatatttga tggacacta cacggattc atttagagg ccagtgcta cgtgcaatc agtgatgca gacgaactg cccagtgat taaaaataa aattttttg tataaatat tttctataa tgttaaaaa cgagtggtc gctacggtt atgaagctc cactgcaga ttttcaggt cagtgaatg agttgtttt ttttcaaag ctgacgaag cgttttttt acaatgttt ccagtgagt gtgaactct ttagacacc tgagatagc tacgaaagt gtcgttcgg catagaatt gtttactta aagtgtaga tatttcgaa tgaatgtat atttggttt taatactaa taatgatgt aaataaatc cggtaacaa aagaaaagg atatgaaat atacgatat tattaaaat tacgcactt tcatgccaa aggtagagt aacgttatc catgtagga cgttaacaa tactcaaac actttaatt agtaggaat ttgacgcat tcggagtat ccaactttt ttttaacct gtttttttc tgaaaaaaa gagacaaat attttatat accatttaa ttggccgca attggagac gaacagtct ttaaaattg ctagtgata acagtaacg aaatacgta acgctgcta caaatcgag tatacgtaa ctaattatt ttaatataa aaatcagag ctatgtaag aacggttag gcaaattaa ataggtttc aaaattgag ttattgaga gtcgaggtt ccagagccc ctatttaag ttggttaac catttgtat acacaacta tttatacta ctataaatt aggatttgc agtggcatg ctgtatatt tgtacctat atggataaa ttagagaat agttctttg tgctgtggt gtaatattt tagattaga agtactaag tttggccga cacttaatt tcggacaaa ttaccattt tgtaaatga acaactgta acataaatt tatcgttta accatcccg cataaaact ttattttga tttaaaaac caacgaaac tttaagtac tttaatact tggatttct gtcttgtgg atttcaact tatatatac cgtacatat aattaatat atgtataaa taaaatatt attgtaaat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
