Status
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Gene information
General information
TC032097
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000881
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7752345..7760164
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032097
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03098

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032097 RA
      MFFYYTQFS IHAFDEEFV LNYSRTKLW GEERYLINT 
      SKCRIRNID PFNEEVKKY YHRENYVPC SNKELLSYV 
      EITDSLTTL HINQSLVTA YSVLKISCC YSSINRIVQ 
      KNRSDDGIS VSECVPFEG SVEITDNVV LVLCKNSLG 
      SEIYKNVHA VVTPRDLPP RKKQLPQTS NFTGVVLVG 
      IDSISKLNL VRTMPKTYT FLEGHDFLN LRGYTKIDD 
      NTFPNLMAI LTGKRWEEV YKHCAPDKN TMNNCDIIW 
      DKFNDLGFI TAYVEDMTE IGTFNYNNR LGFGAPPTD 
      FYFRHYMIA SERLPSIRR SHTCSGPEN TGERIMNLA 
      KDFLTIKHY PKFGLFWMN SFSHDNINL PSSMDEKIV 
      SFLKDIYDR LDNTILVFF SDHGFRFGD IRNTHTGWL 
      EERLPFIYL HFPPTFKQQ YSKEYLNFL INTKRLTTP 
      FDLHMTLQD VLLLANQSY KVRPSQACP KCHSLFQEI 
      QESRTCEDA AIDQHWCVC KGHAYINPL TPIVQKAAD 
      FMVGKINAI VRSGDGGNL CAHYYLKKV TSSGLSDEY 
      QNEANQSVS FLLIMIETR PPAKFEATI EIGAKGNKP 
      DFRLLGDIS RTNRYGDNS KCIKNWLQK YCYCDGLFT 
      SIKSAICHF LNCI
    • >TC032097 RB
      MSFIPHKRN KDVKVFLPE WPMRKNGGA RKCLLLCMV 
      LTLIIMFFY YTQFSIHAF DEEFVLNYS RTKLWGEER 
      YLINTSKCR IRNIDPFNE EVKKYYHRE NYVPCSNKE 
      LLSYVEITD SLTTLHINQ SLVTAYSVL KISCCYSSI 
      NRIVQKNRS DDGISVSEC VPFEGSVEI TDNVVLVLC 
      KNSLGSEIY KNVHAVVTP RDLPPRKKQ LPQTSNFTG 
      VVLVGIDSI SKLNLVRTM PKTYTFLEG HDFLNLRGY 
      TKIDDNTFP NLMAILTGK RWEEVYKHC APDKNTMNN 
      CDIIWDKFN DLGFITAYV EDMTEIGTF NYNNRLGFG 
      APPTDFYFR HYMIASERL PSIRRSHTC SGPENTGER 
      IMNLAKDFL TIKHYPKFG LFWMNSFSH DNINLPSSM 
      DEKIVSFLK DIYDRLDNT ILVFFSDHG FRFGDIRNT 
      HTGWLEERL PFIYLHFPP TFKQQYSKE YLNFLINTK 
      RLTTPFDLH MTLQDVLLL ANQSYKVRP SQACPKCHS 
      LFQEIQESR TCEDAAIDQ HWCVCKGHA YINPLTPIV 
      QKAADFMVG KINAIVRSG DGGNLCAHY YLKKVTSSG 
      LSDEYQNEA NQSVSFLLI MIETRPPAK FEATIEIGA 
      KGNKPDFRL LGDISRTNR YGDNSKCIK NWLQKYCYC 
      DGLFTSIKS AICHFLNCI
    CDS sequence
    • >TC032097 RA
      atgtttttt tattacacc cagtttagt attcacgcc 
      tttgatgaa gaatttgta ctaaattat tcccgaacc 
      aaactatgg ggagaggaa agatactta ataaacacc 
      tcaaagtgt cgaattcgg aacattgat ccttttaat 
      gaggaagta aagaagtat taccatcgc gaaaactat 
      gttccttgt tccaataaa gaactgtta tcttacgtt 
      gaaataacg gactctctc actactttg catataaac 
      caaagcttg gtaacagca tacagtgtt ctcaagatt 
      tcgtgttgt tactcttct attaatcga atcgttcaa 
      aagaacagg tccgacgat gggattagc gtttcagaa 
      tgcgttccc ttcgaaggc agtgtggag ataaccgat 
      aatgtggtt ctagtcctt tgcaaaaac tcgctaggt 
      tctgagatc tacaaaaac gtccatgct gtggtgact 
      cctcgggac ctccccccg cgaaaaaag caacttcct 
      caaacctca aacttcacc ggtgttgtc ttagtggga 
      atcgacagt atttcgaaa ctgaatcta gttcgtacg 
      atgccaaaa acgtacact ttcttagaa gggcacgat 
      tttctaaat ctgagagga tacaccaaa atcgacgac 
      aatactttt cccaatttg atggccatt ttgacgggg 
      aaacggtgg gaagaagtg tacaaacac tgcgccccc 
      gataagaat acgatgaat aactgtgat ataatatgg 
      gataaattt aacgattta ggatttata acggcctat 
      gttgaggat atgacagag attggaacg tttaattac 
      aataatagg ttagggttc ggggctcca ccgaccgac 
      ttttatttc cgacattac atgatagcc agtgaacgg 
      ttgccttca attcggagg tcccacaca tgttccgga 
      ccggaaaac accggtgag agaataatg aatctagcc 
      aaagacttt ctaactatt aaacactac ccgaaattc 
      ggactgttt tggatgaac agcttcagt cacgacaac 
      ataaacttg ccttcctca atggacgag aaaatagtc 
      tctttccta aaagacatt tacgatcgc ttggataac 
      accatcctc gtgttcttc agcgatcac ggcttcagg 
      ttcggagac atccgcaac actcacacc ggatggctg 
      gaggaacgc cttcccttc atctatctc cacttcccc 
      ccaactttc aagcaacag tattccaag gagtaccta 
      aacttcctc attaatacc aaacgtctc actaccccc 
      ttcgacctc cacatgact ctccaagac gttctgctt 
      ctcgcgaat caaagttac aaagtgcgt cctagtcag 
      gcttgcccc aaatgccac tccttgttc caagaaatt 
      caagagtct cgcacttgc gaagacgct gctattgat 
      caacactgg tgcgtatgc aagggtcac gcttacatt 
      aaccccttg accccgatc gtacaaaag gccgccgat 
      tttatggtc gggaaaata aacgcgatt gtgagatca 
      ggcgatgga ggcaactta tgtgcacac tattatttg 
      aaaaaggtc acttcgtcg ggactgtcc gacgagtac 
      cagaatgag gcgaaccag agtgtgagt tttctcctg 
      attatgatc gagacaagg ccaccggcc aagtttgag 
      gccacgatc gaaattggg gcaaagggt aacaagccg 
      gattttcgt ttgttaggt gatattagt cgtacaaat 
      aggtatgga gacaatagc aaatgcatc aagaattgg 
      ttgcagaaa tattgctac tgtgatgga ctattcact 
      tcaattaaa agtgccata tgtcatttt ttgaattgt 
      atttaa
    • >TC032097 RB
      atgagtttc atacctcat aagcgtaat aaagatgtt 
      aaggtattt ttaccagaa tggcctatg agaaaaaac 
      ggaggagcg cggaaatgc ctacttttg tgtatggtt 
      ctaacttta ataattatg tttttttat tacacccag 
      tttagtatt cacgccttt gatgaagaa tttgtacta 
      aattattcc cgaaccaaa ctatgggga gaggaaaga 
      tacttaata aacacctca aagtgtcga attcggaac 
      attgatcct tttaatgag gaagtaaag aagtattac 
      catcgcgaa aactatgtt ccttgttcc aataaagaa 
      ctgttatct tacgttgaa ataacggac tctctcact 
      actttgcat ataaaccaa agcttggta acagcatac 
      agtgttctc aagatttcg tgttgttac tcttctatt 
      aatcgaatc gttcaaaag aacaggtcc gacgatggg 
      attagcgtt tcagaatgc gttcccttc gaaggcagt 
      gtggagata accgataat gtggttcta gtcctttgc 
      aaaaactcg ctaggttct gagatctac aaaaacgtc 
      catgctgtg gtgactcct cgggacctc cccccgcga 
      aaaaagcaa cttcctcaa acctcaaac ttcaccggt 
      gttgtctta gtgggaatc gacagtatt tcgaaactg 
      aatctagtt cgtacgatg ccaaaaacg tacactttc 
      ttagaaggg cacgatttt ctaaatctg agaggatac 
      accaaaatc gacgacaat acttttccc aatttgatg 
      gccattttg acggggaaa cggtgggaa gaagtgtac 
      aaacactgc gcccccgat aagaatacg atgaataac 
      tgtgatata atatgggat aaatttaac gatttagga 
      tttataacg gcctatgtt gaggatatg acagagatt 
      ggaacgttt aattacaat aataggtta gggttcggg 
      gctccaccg accgacttt tatttccga cattacatg 
      atagccagt gaacggttg ccttcaatt cggaggtcc 
      cacacatgt tccggaccg gaaaacacc ggtgagaga 
      ataatgaat ctagccaaa gactttcta actattaaa 
      cactacccg aaattcgga ctgttttgg atgaacagc 
      ttcagtcac gacaacata aacttgcct tcctcaatg 
      gacgagaaa atagtctct ttcctaaaa gacatttac 
      gatcgcttg gataacacc atcctcgtg ttcttcagc 
      gatcacggc ttcaggttc ggagacatc cgcaacact 
      cacaccgga tggctggag gaacgcctt cccttcatc 
      tatctccac ttcccccca actttcaag caacagtat 
      tccaaggag tacctaaac ttcctcatt aataccaaa 
      cgtctcact acccccttc gacctccac atgactctc 
      caagacgtt ctgcttctc gcgaatcaa agttacaaa 
      gtgcgtcct agtcaggct tgccccaaa tgccactcc 
      ttgttccaa gaaattcaa gagtctcgc acttgcgaa 
      gacgctgct attgatcaa cactggtgc gtatgcaag 
      ggtcacgct tacattaac cccttgacc ccgatcgta 
      caaaaggcc gccgatttt atggtcggg aaaataaac 
      gcgattgtg agatcaggc gatggaggc aacttatgt 
      gcacactat tatttgaaa aaggtcact tcgtcggga 
      ctgtccgac gagtaccag aatgaggcg aaccagagt 
      gtgagtttt ctcctgatt atgatcgag acaaggcca 
      ccggccaag tttgaggcc acgatcgaa attggggca 
      aagggtaac aagccggat tttcgtttg ttaggtgat 
      attagtcgt acaaatagg tatggagac aatagcaaa 
      tgcatcaag aattggttg cagaaatat tgctactgt 
      gatggacta ttcacttca attaaaagt gccatatgt 
      cattttttg aattgtatt taa
    mRNA sequence
    • >TC032097 RA
      atccacttg agtaaatcg agtaactcg acgtttaag 
      cgcctcttt gtgtgcaaa cttttacga tgacctact 
      ctccaattt cggtggagc cttacgtaa tttctttga 
      tgccaccta tttttagac cgtaattgc cttaattta 
      cctcaaaca agaacgaca aactcaaat ccatcattt 
      atgtcggca ttgtttaat ttggaatcg aacttgtaa 
      tttagctgt tatcagtcg atatttcct gttgtaaaa 
      accaaccca caactcacg taattttta attaaactg 
      aatggcatc acttattga ttactttag aaattggca 
      atgcatgaa tagcttatg cgaagaatg gcctatgag 
      aaaaaacgg aggagcgcg gaaatgcct acttttgtg 
      tatggttct aactttaat aattatgtt tttttatta 
      cacccagtt tagtattca cgcctttga tgaagaatt 
      tgtactaaa ttattcccg aaccaaact atggggaga 
      ggaaagata cttaataaa cacctcaaa gtgtcgaat 
      tcggaacat tgatccttt taatgagga agtaaagaa 
      gtattacca tcgcgaaaa ctatgttcc ttgttccaa 
      taaagaact gttatctta cgttgaaat aacggactc 
      tctcactac tttgcatat aaaccaaag cttggtaac 
      agcatacag tgttctcaa gatttcgtg ttgttactc 
      ttctattaa tcgaatcgt tcaaaagaa caggtccga 
      cgatgggat tagcgtttc agaatgcgt tcccttcga 
      aggcagtgt ggagataac cgataatgt ggttctagt 
      cctttgcaa aaactcgct aggttctga gatctacaa 
      aaacgtcca tgctgtggt gactcctcg ggacctccc 
      cccgcgaaa aaagcaact tcctcaaac ctcaaactt 
      caccggtgt tgtcttagt gggaatcga cagtatttc 
      gaaactgaa tctagttcg tacgatgcc aaaaacgta 
      cactttctt agaagggca cgattttct aaatctgag 
      aggatacac caaaatcga cgacaatac ttttcccaa 
      tttgatggc cattttgac ggggaaacg gtgggaaga 
      agtgtacaa acactgcgc ccccgataa gaatacgat 
      gaataactg tgatataat atgggataa atttaacga 
      tttaggatt tataacggc ctatgttga ggatatgac 
      agagattgg aacgtttaa ttacaataa taggttagg 
      gttcggggc tccaccgac cgactttta tttccgaca 
      ttacatgat agccagtga acggttgcc ttcaattcg 
      gaggtccca cacatgttc cggaccgga aaacaccgg 
      tgagagaat aatgaatct agccaaaga ctttctaac 
      tattaaaca ctacccgaa attcggact gttttggat 
      gaacagctt cagtcacga caacataaa cttgccttc 
      ctcaatgga cgagaaaat agtctcttt cctaaaaga 
      catttacga tcgcttgga taacaccat cctcgtgtt 
      cttcagcga tcacggctt caggttcgg agacatccg 
      caacactca caccggatg gctggagga acgccttcc 
      cttcatcta tctccactt ccccccaac tttcaagca 
      acagtattc caaggagta cctaaactt cctcattaa 
      taccaaacg tctcactac ccccttcga cctccacat 
      gactctcca agacgttct gcttctcgc gaatcaaag 
      ttacaaagt gcgtcctag tcaggcttg ccccaaatg 
      ccactcctt gttccaaga aattcaaga gtctcgcac 
      ttgcgaaga cgctgctat tgatcaaca ctggtgcgt 
      atgcaaggg tcacgctta cattaaccc cttgacccc 
      gatcgtaca aaaggccgc cgattttat ggtcgggaa 
      aataaacgc gattgtgag atcaggcga tggaggcaa 
      cttatgtgc acactatta tttgaaaaa ggtcacttc 
      gtcgggact gtccgacga gtaccagaa tgaggcgaa 
      ccagagtgt gagttttct cctgattat gatcgagac 
      aaggccacc ggccaagtt tgaggccac gatcgaaat 
      tggggcaaa gggtaacaa gccggattt tcgtttgtt 
      aggtgatat tagtcgtac aaataggta tggagacaa 
      tagcaaatg catcaagaa ttggttgca gaaatattg 
      ctactgtga tggactatt cacttcaat taaaagtgc 
      catatgtca ttttttgaa ttgtattta attttatat 
      ttgttgtat ttacaagag ttatatttt caatgttaa 
      taaagtttg ttttatatt
    • >TC032097 RB
      gtgtttttg gagaaatac cacaattaa ttaaaagtt 
      tgacgtttt tatcagacg tgaagtgct aaactgaga 
      tttatcgtt tagatattt acgaaaaag taaaatgag 
      tttcatacc tcataagcg taataaaga tgttaaggt 
      atttttacc agaatggcc tatgagaaa aaacggagg 
      agcgcggaa atgcctact tttgtgtat ggttctaac 
      tttaataat tatgttttt ttattacac ccagtttag 
      tattcacgc ctttgatga agaatttgt actaaatta 
      ttcccgaac caaactatg gggagagga aagatactt 
      aataaacac ctcaaagtg tcgaattcg gaacattga 
      tccttttaa tgaggaagt aaagaagta ttaccatcg 
      cgaaaacta tgttccttg ttccaataa agaactgtt 
      atcttacgt tgaaataac ggactctct cactacttt 
      gcatataaa ccaaagctt ggtaacagc atacagtgt 
      tctcaagat ttcgtgttg ttactcttc tattaatcg 
      aatcgttca aaagaacag gtccgacga tgggattag 
      cgtttcaga atgcgttcc cttcgaagg cagtgtgga 
      gataaccga taatgtggt tctagtcct ttgcaaaaa 
      ctcgctagg ttctgagat ctacaaaaa cgtccatgc 
      tgtggtgac tcctcggga cctcccccc gcgaaaaaa 
      gcaacttcc tcaaacctc aaacttcac cggtgttgt 
      cttagtggg aatcgacag tatttcgaa actgaatct 
      agttcgtac gatgccaaa aacgtacac tttcttaga 
      agggcacga ttttctaaa tctgagagg atacaccaa 
      aatcgacga caatacttt tcccaattt gatggccat 
      tttgacggg gaaacggtg ggaagaagt gtacaaaca 
      ctgcgcccc cgataagaa tacgatgaa taactgtga 
      tataatatg ggataaatt taacgattt aggatttat 
      aacggccta tgttgagga tatgacaga gattggaac 
      gtttaatta caataatag gttagggtt cggggctcc 
      accgaccga cttttattt ccgacatta catgatagc 
      cagtgaacg gttgccttc aattcggag gtcccacac 
      atgttccgg accggaaaa caccggtga gagaataat 
      gaatctagc caaagactt tctaactat taaacacta 
      cccgaaatt cggactgtt ttggatgaa cagcttcag 
      tcacgacaa cataaactt gccttcctc aatggacga 
      gaaaatagt ctctttcct aaaagacat ttacgatcg 
      cttggataa caccatcct cgtgttctt cagcgatca 
      cggcttcag gttcggaga catccgcaa cactcacac 
      cggatggct ggaggaacg ccttccctt catctatct 
      ccacttccc cccaacttt caagcaaca gtattccaa 
      ggagtacct aaacttcct cattaatac caaacgtct 
      cactacccc cttcgacct ccacatgac tctccaaga 
      cgttctgct tctcgcgaa tcaaagtta caaagtgcg 
      tcctagtca ggcttgccc caaatgcca ctccttgtt 
      ccaagaaat tcaagagtc tcgcacttg cgaagacgc 
      tgctattga tcaacactg gtgcgtatg caagggtca 
      cgcttacat taacccctt gaccccgat cgtacaaaa 
      ggccgccga ttttatggt cgggaaaat aaacgcgat 
      tgtgagatc aggcgatgg aggcaactt atgtgcaca 
      ctattattt gaaaaaggt cacttcgtc gggactgtc 
      cgacgagta ccagaatga ggcgaacca gagtgtgag 
      ttttctcct gattatgat cgagacaag gccaccggc 
      caagtttga ggccacgat cgaaattgg ggcaaaggg 
      taacaagcc ggattttcg tttgttagg tgatattag 
      tcgtacaaa taggtatgg agacaatag caaatgcat 
      caagaattg gttgcagaa atattgcta ctgtgatgg 
      actattcac ttcaattaa aagtgccat atgtcattt 
      tttgaattg tatttaatt ttatatttg ttgtattta 
      caagagtta tattttcaa tgttaataa agtttgttt 
      tatatt
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