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Gene information
General information
TC000877 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7679811..7681477
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000877
    iBeetle screen iB_03097

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000877 RA
      MNIRLFISE IKKRPVLWD HSNRHYYSK 
NALNDGWLE IAKMFDIEV YSAKQKWKN 
LRDTFRVEL KKTKRYRSS DGTSRCIWA 
YYEDMYFIK DILRTRRAP DPELPTASP 
EITEVANET IKTEKDSVT YSPSRLTDD 
ASNIVIENV TSLHADSDV DTTNEQIDQ 
RGVKRKWFK EEQTEGNED DQDDDLLFF 
RSLLPFVKK LDQDKKLLF RMNVQQMLY 
DQLYNN
    CDS sequence
    • >TC000877 RA
      atgaatatt cgcttgttt atcagtgaa attaaaaaa 
aggccagtt ctgtgggac cattccaac cggcattat 
tacagcaag aatgcctta aacgacgga tggctcgaa 
atcgcgaaa atgttcgac atcgaagtt tattcagct 
aaacagaaa tggaagaac ctgagagac acatttcgc 
gtcgaactg aagaaaacc aagaggtac agatcttca 
gacgggacg tcgaggtgc atttgggcc tattatgaa 
gatatgtac ttcattaag gatatccta aggacaaga 
cgagcacca gatcccgaa ctaccaact gctagccct 
gaaataacc gaagtcgcc aatgaaacc atcaagact 
gagaaagac tcagtcact tattcgcca agtcgccta 
actgacgat gcctcaaac attgttatt gaaaatgtt 
acctcgcta catgccgac agtgatgtc gatacgaca 
aacgagcag attgaccaa agaggggtt aagaggaaa 
tggttcaag gaggaacaa acggaaggg aatgaggac 
gaccaagat gacgactta ctgttcttt cggagtttg 
cttcccttt gtcaaaaaa ttggaccaa gacaaaaaa 
ttgttgttc cggatgaat gtccaacaa atgttgtat 
gaccagttg tataataat taa
    mRNA sequence
    • >TC000877 RA
      ttcggcgaa aatcatcaa aaaccacca caagagtgt 
atttttctg gacaattgt ggggtgtct caggagttg 
cgttttcaa taaactagc ctagatgtg tttccgaaa 
atgggagag gggcgggtc cggaaggcc tgggataat 
ctgatcggg ggcatatta acaatagtc aattaaaat 
atcgccctc tcatccctt tgtaatgat atttgaatg 
aaaaactaa accgggatg agggtggtc acgtggtgg 
tgtatcccg tgacgtaac ctccagtcc caaaaacca 
ttctggcag tggagcaaa tttccctaa agcgcaatc 
aatcttgtg tcagtcgat gaagagcca tttccgcca 
aataaaata aaacaatcg ctggctaaa tgtaatcga 
agaatagaa cggaacaaa agcagtctg gcttcgtgt 
aatcaactc agatattgg gggattcac ttagcaggg 
gttgaaggg aaacctgcg agtattgtc tcacatatg 
ggctccctt catttgcca acaactcaa ccaagacta 
tatcgatta ttgtttggc tatcgcccc gaattaaca 
cagagtcgc tggttcctc gaccttccg ccaaaaaat 
atttgcgaa agttatgtc tgttattcg gtttaacgg 
tctgcgagc gaacgatcg ttgacatcg agtcgacgt 
gatggaaga atcacttcg cagcgaatc aatgcgcac 
gcattgctt tctcgcgaa tagacgagc gaccgttgg 
cgtcgaaca aaggtcggt gatacacgc gtttaacaa 
tgaatattc gcttgttta tcagtgaaa ttaaaaaaa 
ggccagttc tgtgggacc attccaacc ggcattatt 
acagcaaga atgccttaa acgacggat ggctcgaaa 
tcgcgaaaa tgttcgaca tcgaagttt attcagcta 
aacagaaat ggaagaacc tgagagaca catttcgcg 
tcgaactga agaaaacca agaggtaca gatcttcag 
acgggacgt cgaggtgca tttgggcct attatgaag 
atatgtact tcattaagg atatcctaa ggacaagac 
gagcaccag atcccgaac taccaactg ctagccctg 
aaataaccg aagtcgcca atgaaacca tcaagactg 
agaaagact cagtcactt attcgccaa gtcgcctaa 
ctgacgatg cctcaaaca ttgttattg aaaatgtta 
cctcgctac atgccgaca gtgatgtcg atacgacaa 
acgagcaga ttgaccaaa gaggggtta agaggaaat 
ggttcaagg aggaacaaa cggaaggga atgaggacg 
accaagatg acgacttac tgttctttc ggagtttgc 
ttccctttg tcaaaaaat tggaccaag acaaaaaat 
tgttgttcc ggatgaatg tccaacaaa tgttgtatg 
accagttgt ataataatt aataaatta agtttcttc 
ttgttttgc gtttttttc tccttcagg tgacaagac 
attggataa caaaaacaa tattaattt agaaaaccg 
cagtaataa aatattcgc agcttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY