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Gene information
General information
TC000872 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7578571..7580205
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000872
    iBeetle screen iB_03096

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000872 RA
      MALPESSES SEKKPDSKG QDKKEKLLT KVVIRRLPP 
NMDQETFLN QISPVPSYD YLYMVKGDA SLGENSFSR 
AYINFVNPN DIYDFKEKF DNYVFLDSA GHEYAAVVE 
FAAFQKIPK RRNKVRVDP KVGTIESDP YYLEFVEMI 
NKPPEPDEK PEYSYQITT ENKNETSTP LLEYVKNKR 
VEKMRIREE RREERKRKE FERKKFRED ERRKRYEEK 
SPVKSKFTK VVSPKEKNV DNKEVEKHE GEDKSTEDK 
TPEKEEPEK YEKPAPTSY YSKNRDKKY DDRKKDVKN 
KYPPKKEYL DKREYKGRR DDYKDYKER DYRSKYDDS 
KKEDTKVYQ KKVKKYTEK REERKIEAQ KRLEQQQEN 
SPKTEENVK SEQQPKESL SKKEESSLV HEASQETST 
KSSEKSKTT KDSDAQSQK RIRNKDRPT IAIYRPGML 
SKRKQNEGE SDHKEGKKE
    CDS sequence
    • >TC000872 RA
      atggcatta cctgagagc agcgagtcc tcggaaaaa 
aagcccgac tccaagggt caggacaaa aaagagaaa 
cttctcacg aaagtggtg attcgtcgt ttgccccca 
aatatggat caagagact tttctcaac caaatatcg 
cctgttccc agctacgac tacttgtac atggttaag 
ggtgacgcc agtttgggc gaaaattcc ttttcccgt 
gcctacatt aattttgtc aaccccaat gacatttac 
gattttaag gaaaagttc gacaattac gtttttcta 
gacagtgct ggtcatgag tatgcggca gtggtcgaa 
tttgctgcc tttcaaaaa attccaaaa aggcgaaat 
aaagtaagg gttgaccct aaagttgga actattgaa 
tcagaccct tactacttg gaattcgtt gaaatgata 
aataaacca ccagaacct gacgaaaaa cccgagtat 
tcctatcag attacaacc gagaataag aacgaaaca 
agcacacct ttgttagag tatgtgaaa aacaaaaga 
gtcgaaaaa atgaggatt cgtgaggag agaagagaa 
gagaggaaa cggaaggaa tttgaaaga aagaagttt 
cgtgaagat gaacggagg aaacggtat gaggaaaaa 
tcgcccgtc aagagtaaa tttacaaaa gttgtgagt 
cctaaagag aaaaatgtg gataataag gaagtggag 
aaacatgag ggtgaggat aaatcaact gaagataaa 
acaccagag aaagaagaa cctgagaaa tacgaaaag 
ccggctcca acatcttac tacagtaaa aacagggac 
aagaaatat gatgacagg aagaaagat gtgaagaac 
aagtatcca ccaaagaag gagtacttg gataaaagg 
gaatacaaa ggaaggaga gacgactat aaagattac 
aaagaaaga gactatagg tcaaaatac gacgacagc 
aagaaagag gataccaaa gtttaccaa aagaaggtc 
aagaagtac actgagaag agagaagaa agaaaaatt 
gaagcccag aaaagactt gagcagcaa caggaaaac 
agcccaaaa actgaagaa aacgtcaaa tcggagcaa 
caacctaaa gaaagtcta agcaaaaag gaagaaagt 
tcgctcgtt catgaagca agtcaagaa acatctact 
aagtcatcc gaaaaatca aaaacaaca aaggatagt 
gatgcacaa tcccagaag cgaatcagg aacaaggat 
aggcccaca attgccatc tacaggccc ggaatgttg 
tcaaaacgg aagcagaac gaaggagaa agtgatcat 
aaggaaggc aaaaaagaa taa
    mRNA sequence
    • >TC000872 RA
      cacttctag aaatataac aaatgacac taatgaagt 
aatgacagc tgacaaaga attttaggt tagaattag 
gattttgtt atcattgtc aatcagtca accacttac 
ttatcacag cttatcagt cagtcttag tacagtttt 
gttacatag aagcagatt acacaatta tggcattac 
ctgagagca gcgagtcct cggaaaaaa agcccgact 
ccaagggtc aggacaaaa aagagaaac ttctcacga 
aagtggtga ttcgtcgtt tgcccccaa atatggatc 
aagagactt ttctcaacc aaatatcgc ctgttccca 
gctacgact acttgtaca tggttaagg gtgacgcca 
gtttgggcg aaaattcct tttcccgtg cctacatta 
attttgtca accccaatg acatttacg attttaagg 
aaaagttcg acaattacg tttttctag acagtgctg 
gtcatgagt atgcggcag tggtcgaat ttgctgcct 
ttcaaaaaa ttccaaaaa ggcgaaata aagtaaggg 
ttgacccta aagttggaa ctattgaat cagaccctt 
actacttgg aattcgttg aaatgataa ataaaccac 
cagaacctg acgaaaaac ccgagtatt cctatcaga 
ttacaaccg agaataaga acgaaacaa gcacacctt 
tgttagagt atgtgaaaa acaaaagag tcgaaaaaa 
tgaggattc gtgaggaga gaagagaag agaggaaac 
ggaaggaat ttgaaagaa agaagtttc gtgaagatg 
aacggagga aacggtatg aggaaaaat cgcccgtca 
agagtaaat ttacaaaag ttgtgagtc ctaaagaga 
aaaatgtgg ataataagg aagtggaga aacatgagg 
gtgaggata aatcaactg aagataaaa caccagaga 
aagaagaac ctgagaaat acgaaaagc cggctccaa 
catcttact acagtaaaa acagggaca agaaatatg 
atgacagga agaaagatg tgaagaaca agtatccac 
caaagaagg agtacttgg ataaaaggg aatacaaag 
gaaggagag acgactata aagattaca aagaaagag 
actataggt caaaatacg acgacagca agaaagagg 
ataccaaag tttaccaaa agaaggtca agaagtaca 
ctgagaaga gagaagaaa gaaaaattg aagcccaga 
aaagacttg agcagcaac aggaaaaca gcccaaaaa 
ctgaagaaa acgtcaaat cggagcaac aacctaaag 
aaagtctaa gcaaaaagg aagaaagtt cgctcgttc 
atgaagcaa gtcaagaaa catctacta agtcatccg 
aaaaatcaa aaacaacaa aggatagtg atgcacaat 
cccagaagc gaatcagga acaaggata ggcccacaa 
ttgccatct acaggcccg gaatgttgt caaaacgga 
agcagaacg aaggagaaa gtgatcata aggaaggca 
aaaaagaat aattttttc tctagaatt gtagttttt 
ttgtttgcc aataaatga agtgtgcta cttatcgaa 
ttatcgtaa ttatttccg acctttttg taaaaagaa 
aattgtaaa aaaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY