Status
Please wait ...
Gene information
General information
TC000872 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 2072028..2073662
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000872
    iBeetle screen iB_03096
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000872 RA
      MALPESSES SEKKPDSKG QDKKEKLLT KVVIRRLPP 
      NMDQETFLN QISPVPSYD YLYMVKGDA SLGENSFSR 
      AYINFVNPN DIYDFKEKF DNYVFLDSA GHEYAAVVE 
      FAAFQKIPK RRNKVRVDP KVGTIESDP YYLEFVEMI 
      NKPPEPDEK PEYSYQITT ENKNETSTP LLEYVKNKR 
      VEKMRIREE RREERKRKE FERKKFRED ERRKRYEEK 
      SPVKSKFTK VVSPKEKNV DNKEVEKHE GEDKSTEDK 
      TPEKEEPEK YEKPAPTSY YSKNRDKKY DDRKKDVKN 
      KYPPKKEYL DKREYKGRR DDYKDYKER DYRSKYDDS 
      KKEDTKVYQ KKVKKYTEK REERKIEAQ KRLEQQQEN 
      SPKTEENVK SEQQPKESL SKKEESSLV HEASQETST 
      KSSEKSKTT KDSDAQSQK RIRNKDRPT IAIYRPGML 
      SKRKQNEGE SDHKEGKKE
    CDS sequence
    • >TC000872 RA
      atggcatta cctgagagc agcgagtcc tcggaaaaa 
      aagcccgac tccaagggt caggacaaa aaagagaaa 
      cttctcacg aaagtggtg attcgtcgt ttgccccca 
      aatatggat caagagact tttctcaac caaatatcg 
      cctgttccc agctacgac tacttgtac atggttaag 
      ggtgacgcc agtttgggc gaaaattcc ttttcccgt 
      gcctacatt aattttgtc aaccccaat gacatttac 
      gattttaag gaaaagttc gacaattac gtttttcta 
      gacagtgct ggtcatgag tatgcggca gtggtcgaa 
      tttgctgcc tttcaaaaa attccaaaa aggcgaaat 
      aaagtaagg gttgaccct aaagttgga actattgaa 
      tcagaccct tactacttg gaattcgtt gaaatgata 
      aataaacca ccagaacct gacgaaaaa cccgagtat 
      tcctatcag attacaacc gagaataag aacgaaaca 
      agcacacct ttgttagag tatgtgaaa aacaaaaga 
      gtcgaaaaa atgaggatt cgtgaggag agaagagaa 
      gagaggaaa cggaaggaa tttgaaaga aagaagttt 
      cgtgaagat gaacggagg aaacggtat gaggaaaaa 
      tcgcccgtc aagagtaaa tttacaaaa gttgtgagt 
      cctaaagag aaaaatgtg gataataag gaagtggag 
      aaacatgag ggtgaggat aaatcaact gaagataaa 
      acaccagag aaagaagaa cctgagaaa tacgaaaag 
      ccggctcca acatcttac tacagtaaa aacagggac 
      aagaaatat gatgacagg aagaaagat gtgaagaac 
      aagtatcca ccaaagaag gagtacttg gataaaagg 
      gaatacaaa ggaaggaga gacgactat aaagattac 
      aaagaaaga gactatagg tcaaaatac gacgacagc 
      aagaaagag gataccaaa gtttaccaa aagaaggtc 
      aagaagtac actgagaag agagaagaa agaaaaatt 
      gaagcccag aaaagactt gagcagcaa caggaaaac 
      agcccaaaa actgaagaa aacgtcaaa tcggagcaa 
      caacctaaa gaaagtcta agcaaaaag gaagaaagt 
      tcgctcgtt catgaagca agtcaagaa acatctact 
      aagtcatcc gaaaaatca aaaacaaca aaggatagt 
      gatgcacaa tcccagaag cgaatcagg aacaaggat 
      aggcccaca attgccatc tacaggccc ggaatgttg 
      tcaaaacgg aagcagaac gaaggagaa agtgatcat 
      aaggaaggc aaaaaagaa taa
    mRNA sequence
    • >TC000872 RA
      cacttctag aaatataac aaatgacac taatgaagt 
      aatgacagc tgacaaaga attttaggt tagaattag 
      gattttgtt atcattgtc aatcagtca accacttac 
      ttatcacag cttatcagt cagtcttag tacagtttt 
      gttacatag aagcagatt acacaatta tggcattac 
      ctgagagca gcgagtcct cggaaaaaa agcccgact 
      ccaagggtc aggacaaaa aagagaaac ttctcacga 
      aagtggtga ttcgtcgtt tgcccccaa atatggatc 
      aagagactt ttctcaacc aaatatcgc ctgttccca 
      gctacgact acttgtaca tggttaagg gtgacgcca 
      gtttgggcg aaaattcct tttcccgtg cctacatta 
      attttgtca accccaatg acatttacg attttaagg 
      aaaagttcg acaattacg tttttctag acagtgctg 
      gtcatgagt atgcggcag tggtcgaat ttgctgcct 
      ttcaaaaaa ttccaaaaa ggcgaaata aagtaaggg 
      ttgacccta aagttggaa ctattgaat cagaccctt 
      actacttgg aattcgttg aaatgataa ataaaccac 
      cagaacctg acgaaaaac ccgagtatt cctatcaga 
      ttacaaccg agaataaga acgaaacaa gcacacctt 
      tgttagagt atgtgaaaa acaaaagag tcgaaaaaa 
      tgaggattc gtgaggaga gaagagaag agaggaaac 
      ggaaggaat ttgaaagaa agaagtttc gtgaagatg 
      aacggagga aacggtatg aggaaaaat cgcccgtca 
      agagtaaat ttacaaaag ttgtgagtc ctaaagaga 
      aaaatgtgg ataataagg aagtggaga aacatgagg 
      gtgaggata aatcaactg aagataaaa caccagaga 
      aagaagaac ctgagaaat acgaaaagc cggctccaa 
      catcttact acagtaaaa acagggaca agaaatatg 
      atgacagga agaaagatg tgaagaaca agtatccac 
      caaagaagg agtacttgg ataaaaggg aatacaaag 
      gaaggagag acgactata aagattaca aagaaagag 
      actataggt caaaatacg acgacagca agaaagagg 
      ataccaaag tttaccaaa agaaggtca agaagtaca 
      ctgagaaga gagaagaaa gaaaaattg aagcccaga 
      aaagacttg agcagcaac aggaaaaca gcccaaaaa 
      ctgaagaaa acgtcaaat cggagcaac aacctaaag 
      aaagtctaa gcaaaaagg aagaaagtt cgctcgttc 
      atgaagcaa gtcaagaaa catctacta agtcatccg 
      aaaaatcaa aaacaacaa aggatagtg atgcacaat 
      cccagaagc gaatcagga acaaggata ggcccacaa 
      ttgccatct acaggcccg gaatgttgt caaaacgga 
      agcagaacg aaggagaaa gtgatcata aggaaggca 
      aaaaagaat aattttttc tctagaatt gtagttttt 
      ttgtttgcc aataaatga agtgtgcta cttatcgaa 
      ttatcgtaa ttatttccg acctttttg taaaaagaa 
      aattgtaaa aaaaca