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Gene information
General information
TC000863 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7257804..7287151
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000863
    iBeetle screen iB_03092

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000863 RA
      MAEEGARFR SILVLAVVV AVAADYLEN PKFSAPITN 
      VTVPVGREA ILACLVEDL GGYKVAWLR VDTQTILTI 
      HSHVITKNH RIGVTHSEH KTWYLHIKV VRESDRGWY 
      MCQINTDPM KSQIGYLDV VVPPDILDY PTSTDMVVR 
      EGSNVNLRC VANGSPEPS INWRRETGE PLKLLTGEE 
      VPSVDGPVL NISRVSRQQ MGPYLCIAS NGIPPSVSK 
      RIMLIVHFP PMIWIQNQL VGAYEGQQI TLECHSEAF 
      PKSINYWTT DKGEIVPQS GKYEPSYST NGYKIHMKL 
      TIRSITPQD YGNYKCVSK NSLGDTDGT IQVFAIPKP 
      SKLSNGLSR KGKLKKKPN DVIGELENI ADGLDDQPD 
      SLFQEEEYG SGERAKTGL IFVAFLVAL PLVLQ
    • >TC000863 RB
      MAEEGARFR SILVLAVVV AVAADYLEN PKFSAPITN 
      VTVPVGREA ILACLVEDL GGYKVAWLR VDTQTILTI 
      HSHVITKNH RIGVTHSEH KTWYLHIKV VRESDRGWY 
      MCQINTDPM KSQIGYLDV VVPPDILDY PTSTDMVVR 
      EGSNVNLRC VANGSPEPS INWRRETGE PLKLLTGEE 
      VPSVDGPVL NISRVSRQQ MGPYLCIAS NGIPPSVSK 
      RIMLIVHFP PMIWIQNQL VGAYEGQQI TLECHSEAF 
      PKSINYWTT DKGEIVPQS GKYEPSYST NGYKIHMKL 
      TIRSITPQD YGNYKCVSK NSLGDTDGT IQVFAIPKP 
      SKLSNGLSR KGLDDQPDS LFQEEEYGS GERAKTGLI 
      FVAFLVALP LVLQ
    CDS sequence
    • >TC000863 RA
      atggctgaa gaaggcgcg cgttttcgg tccatcttg 
      gttctggca gtggtcgtc gcagtggca gccgattat 
      ctagaaaat cccaaattc agtgcaccc atcaccaac 
      gtcaccgta ccagttggc agagaagcc atccttgct 
      tgtcttgtc gaagattta ggaggatat aaggtggca 
      tggctgcgt gtggacacc cagaccata ctgaccatc 
      cacagtcat gtgataacg aagaaccac cgaatcggt 
      gtgacccac agcgaacac aaaacatgg tacttgcac 
      ataaaagtc gtgcgtgag tcggaccga gggtggtac 
      atgtgtcag ataaacacc gaccccatg aaaagccaa 
      attggatat ctggatgtg gtcgtgcct ccggatatc 
      ctggactac cctacgagc accgatatg gtggtgcgt 
      gagggatcc aacgtgaat cttcgctgt gttgccaat 
      ggatcacca gaaccttca atcaactgg agacgagaa 
      accggagag cccctcaaa ttgttaacg ggagaagaa 
      gtgccaagt gtcgacggt cctgtgttg aacatcagt 
      cgcgtgagt cggcaacaa atggggcct tacctgtgc 
      atcgcctca aacggcatt cctccatca gtcagcaag 
      cgcatcatg ctcatcgtg cattttcct ccaatgatc 
      tggattcag aatcagcta gtgggagca tacgaggga 
      caacagata acactcgaa tgccactcg gaggctttt 
      ccgaagtct attaactac tggaccact gataaaggc 
      gaaatcgtt ccgcaaagt ggaaaatac gaaccatcg 
      tactcgacg aacggctac aagatccac atgaaactg 
      accatacgg tcgataact ccgcaggac tacgggaat 
      tacaagtgt gtatcgaaa aactcttta ggcgatacg 
      gacggaacg atacaagtt ttcgccata cctaagcca 
      tcaaaactt tcgaatggt ttatcaagg aaaggcaaa 
      cttaaaaag aaacccaac gacgtcatc ggagaactg 
      gaaaatatc gccgacggg ttggacgac caaccggat 
      tccttgttc caagaagag gagtacggt tccggggaa 
      cgtgcaaaa accggactt atcttcgta gctttttta 
      gtcgcgctg cctttggtc ctacaataa
    • >TC000863 RB
      atggctgaa gaaggcgcg cgttttcgg tccatcttg 
      gttctggca gtggtcgtc gcagtggca gccgattat 
      ctagaaaat cccaaattc agtgcaccc atcaccaac 
      gtcaccgta ccagttggc agagaagcc atccttgct 
      tgtcttgtc gaagattta ggaggatat aaggtggca 
      tggctgcgt gtggacacc cagaccata ctgaccatc 
      cacagtcat gtgataacg aagaaccac cgaatcggt 
      gtgacccac agcgaacac aaaacatgg tacttgcac 
      ataaaagtc gtgcgtgag tcggaccga gggtggtac 
      atgtgtcag ataaacacc gaccccatg aaaagccaa 
      attggatat ctggatgtg gtcgtgcct ccggatatc 
      ctggactac cctacgagc accgatatg gtggtgcgt 
      gagggatcc aacgtgaat cttcgctgt gttgccaat 
      ggatcacca gaaccttca atcaactgg agacgagaa 
      accggagag cccctcaaa ttgttaacg ggagaagaa 
      gtgccaagt gtcgacggt cctgtgttg aacatcagt 
      cgcgtgagt cggcaacaa atggggcct tacctgtgc 
      atcgcctca aacggcatt cctccatca gtcagcaag 
      cgcatcatg ctcatcgtg cattttcct ccaatgatc 
      tggattcag aatcagcta gtgggagca tacgaggga 
      caacagata acactcgaa tgccactcg gaggctttt 
      ccgaagtct attaactac tggaccact gataaaggc 
      gaaatcgtt ccgcaaagt ggaaaatac gaaccatcg 
      tactcgacg aacggctac aagatccac atgaaactg 
      accatacgg tcgataact ccgcaggac tacgggaat 
      tacaagtgt gtatcgaaa aactcttta ggcgatacg 
      gacggaacg atacaagtt ttcgccata cctaagcca 
      tcaaaactt tcgaatggt ttatcaagg aaagggttg 
      gacgaccaa ccggattcc ttgttccaa gaagaggag 
      tacggttcc ggggaacgt gcaaaaacc ggacttatc 
      ttcgtagct tttttagtc gcgctgcct ttggtccta 
      caataa
    mRNA sequence
    • >TC000863 RA
      ttcagcctg aacccgacg ttcggtcta atggctgaa 
      gaaggcgcg cgttttcgg tccatcttg gttctggca 
      gtggtcgtc gcagtggca gccgattat ctagaaaat 
      cccaaattc agtgcaccc atcaccaac gtcaccgta 
      ccagttggc agagaagcc atccttgct tgtcttgtc 
      gaagattta ggaggatat aaggtggca tggctgcgt 
      gtggacacc cagaccata ctgaccatc cacagtcat 
      gtgataacg aagaaccac cgaatcggt gtgacccac 
      agcgaacac aaaacatgg tacttgcac ataaaagtc 
      gtgcgtgag tcggaccga gggtggtac atgtgtcag 
      ataaacacc gaccccatg aaaagccaa attggatat 
      ctggatgtg gtcgtgcct ccggatatc ctggactac 
      cctacgagc accgatatg gtggtgcgt gagggatcc 
      aacgtgaat cttcgctgt gttgccaat ggatcacca 
      gaaccttca atcaactgg agacgagaa accggagag 
      cccctcaaa ttgttaacg ggagaagaa gtgccaagt 
      gtcgacggt cctgtgttg aacatcagt cgcgtgagt 
      cggcaacaa atggggcct tacctgtgc atcgcctca 
      aacggcatt cctccatca gtcagcaag cgcatcatg 
      ctcatcgtg cattttcct ccaatgatc tggattcag 
      aatcagcta gtgggagca tacgaggga caacagata 
      acactcgaa tgccactcg gaggctttt ccgaagtct 
      attaactac tggaccact gataaaggc gaaatcgtt 
      ccgcaaagt ggaaaatac gaaccatcg tactcgacg 
      aacggctac aagatccac atgaaactg accatacgg 
      tcgataact ccgcaggac tacgggaat tacaagtgt 
      gtatcgaaa aactcttta ggcgatacg gacggaacg 
      atacaagtt ttcgccata cctaagcca tcaaaactt 
      tcgaatggt ttatcaagg aaaggcaaa cttaaaaag 
      aaacccaac gacgtcatc ggagaactg gaaaatatc 
      gccgacggg ttggacgac caaccggat tccttgttc 
      caagaagag gagtacggt tccggggaa cgtgcaaaa 
      accggactt atcttcgta gctttttta gtcgcgctg 
      cctttggtc ctacaataa atcttactc atgttgtgc 
      tgtgtacaa gagaaatct caagattaa cgctgaaac 
      aagatgtgt tgttcagac gatgaattt ctcttcctt 
      aactatttt ttatatgct tccctcgct acaagtggt 
      tttagtgat aaggtccat ttggggcac atgtggccg 
      gaatcattg ttttttttt ctcgcatat atcatacac 
      atttgtcag acagtgcga atgtctgtt agtggctgc 
      gactgataa ctgtattct ttgtaaggt aattagtaa 
      gcaatagcg ctttagaaa taaaattat tggcagtat 
      gtataataa taggtggat tgaatgcag tcagggccc 
      cataaatat ggattacaa gcgacatgc aagtactta 
      gtgtagcaa agttatgca catactaat gataaaacg 
      aattttgac aaaggccaa tcactggcc acaaacctg 
      gtctctcgt tgagcttct cgtggctat agattctgt 
      ctctgtgtt taaataaaa acaaattta gtgttcaag 
      agtctgaaa gtcgatatg catatcctc aatcaaccg 
      agtcaaatg tttcttcct atttaggtt gtttattct 
      ctttgtgta taaataacg agtatgtga atagccgta 
      tcattccat tttgtattg tgaaaaact tgctactaa 
      aataaaata gttttctaa ag
    • >TC000863 RB
      ttcagcctg aacccgacg ttcggtcta atggctgaa 
      gaaggcgcg cgttttcgg tccatcttg gttctggca 
      gtggtcgtc gcagtggca gccgattat ctagaaaat 
      cccaaattc agtgcaccc atcaccaac gtcaccgta 
      ccagttggc agagaagcc atccttgct tgtcttgtc 
      gaagattta ggaggatat aaggtggca tggctgcgt 
      gtggacacc cagaccata ctgaccatc cacagtcat 
      gtgataacg aagaaccac cgaatcggt gtgacccac 
      agcgaacac aaaacatgg tacttgcac ataaaagtc 
      gtgcgtgag tcggaccga gggtggtac atgtgtcag 
      ataaacacc gaccccatg aaaagccaa attggatat 
      ctggatgtg gtcgtgcct ccggatatc ctggactac 
      cctacgagc accgatatg gtggtgcgt gagggatcc 
      aacgtgaat cttcgctgt gttgccaat ggatcacca 
      gaaccttca atcaactgg agacgagaa accggagag 
      cccctcaaa ttgttaacg ggagaagaa gtgccaagt 
      gtcgacggt cctgtgttg aacatcagt cgcgtgagt 
      cggcaacaa atggggcct tacctgtgc atcgcctca 
      aacggcatt cctccatca gtcagcaag cgcatcatg 
      ctcatcgtg cattttcct ccaatgatc tggattcag 
      aatcagcta gtgggagca tacgaggga caacagata 
      acactcgaa tgccactcg gaggctttt ccgaagtct 
      attaactac tggaccact gataaaggc gaaatcgtt 
      ccgcaaagt ggaaaatac gaaccatcg tactcgacg 
      aacggctac aagatccac atgaaactg accatacgg 
      tcgataact ccgcaggac tacgggaat tacaagtgt 
      gtatcgaaa aactcttta ggcgatacg gacggaacg 
      atacaagtt ttcgccata cctaagcca tcaaaactt 
      tcgaatggt ttatcaagg aaagggttg gacgaccaa 
      ccggattcc ttgttccaa gaagaggag tacggttcc 
      ggggaacgt gcaaaaacc ggacttatc ttcgtagct 
      tttttagtc gcgctgcct ttggtccta caataaatc 
      ttactcatg ttgtgctgt gtacaagag aaatctcaa 
      gattaacgc tgaaacaag atgtgttgt tcagacgat 
      gaatttctc ttccttaac tatttttta tatgcttcc 
      ctcgctaca agtggtttt agtgataag gtccatttg 
      gggcacatg tggccggaa tcattgttt ttttttctc 
      gcatatatc atacacatt tgtcagaca gtgcgaatg 
      tctgttagt ggctgcgac tgataactg tattctttg 
      taaggtaat tagtaagca atagcgctt tagaaataa 
      aattattgg cagtatgta taataatag gtggattga 
      atgcagtca gggccccat aaatatgga ttacaagcg 
      acatgcaag tacttagtg tagcaaagt tatgcacat 
      actaatgat aaaacgaat tttgacaaa ggccaatca 
      ctggccaca aacctggtc tctcgttga gcttctcgt 
      ggctataga ttctgtctc tgtgtttaa ataaaaaca 
      aatttagtg ttcaagagt ctgaaagtc gatatgcat 
      atcctcaat caaccgagt caaatgttt cttcctatt 
      taggttgtt tattctctt tgtgtataa ataacgagt 
      atgtgaata gccgtatca ttccatttt gtattgtga 
      aaaacttgc tactaaaat aaaatagtt ttctaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY