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Gene information
General information
TC000849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6789465..6790943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000849
    iBeetle screen iB_03089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late mostlynot present - Number of animals showing the phenotype: 4

    Comment: Females potentially too young.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000849 RA
      MTFMSVAHY LQLVERYWR SQDGAKVAS FLSLRHEHA 
      GYANYHIES PEGIVERFL TAPIDELVI LHLKCLFAI 
      SQSDYLSAY NIQSNLTQT FTKILQSQK EENWSLPIM 
      YVLCLDLRL LAQLAENQR SGINEKPGE MLEKAAECL 
      MGCFRVCAA DNRSAEEDT KRIGMLALV NQLFKVYFR 
      INKLHLCKP LIRAIESSP FKESFSLSQ QITYRYFVG 
      RKAMFDSDY KVADEYLTY AFENCHKLS KKNKTLILI 
      YLVPVKMLL GYIPSKEVL EKYDVIQLW DLVQAVCQG 
      NLKAFDEIM EKHETFFIK CGIYLIVDK LKIIAYRNL 
      FKKVYLILN THQIPIEAL QTALVYLGQ TDVDLDETE 
      CIVANLIYE GKIKGYISH QHRKVVVSK QNPFPPLTS 
      LS
    CDS sequence
    • >TC000849 RA
      atgactttt atgtccgtt gctcattac ctacaactt 
      gtggaaagg tattggcgg tctcaggac ggtgcaaaa 
      gtggccagt tttctgtct cttcgccac gaacatgca 
      ggttatgcc aattaccac attgagagt cctgaggga 
      atagttgag cgttttttg accgcccct atcgacgaa 
      ttagtgatt ttgcaccta aaatgcctg tttgccata 
      agccagagt gactatcta tcggcttat aacattcag 
      agcaatttg acgcaaact ttcactaag atcttgcaa 
      tcgcaaaag gaggagaat tggtctttg cctattatg 
      tatgtcttg tgcttagac ttgcgtctt ttggcgcaa 
      ttagccgag aatcagagg agcggaatt aatgagaag 
      cccggcgaa atgctcgag aaggcagca gaatgcttg 
      atgggttgt ttcagggtg tgtgctgct gataatagg 
      tcagcagag gaggataca aagaggata gggatgttg 
      gctttggtg aatcaattg tttaaggtt tattttcgg 
      ataaataag ctacatttg tgcaagccc ttaatcaga 
      gcgattgag tcatcaccg tttaaagag agtttttct 
      ctgagccaa caaatcaca tatcggtat tttgtgggg 
      cgcaaggct atgtttgat agcgattat aaagtcgcc 
      gatgagtat ttaacttat gcttttgaa aattgtcac 
      aagctcagt aagaagaac aaaacgctt atattgatt 
      tacttagtt cctgtgaaa atgttgttg gggtatatc 
      ccaagcaag gaggttctg gagaagtat gacgtcatc 
      cagttgtgg gatttagta caagctgta tgtcagggt 
      aatctcaaa gcgtttgac gagattatg gaaaagcac 
      gaaaccttt ttcatcaag tgtggaata tatctgatt 
      gttgataaa ctgaaaatc atagcatat agaaatttg 
      tttaaaaag gtgtatttg attctaaat acgcatcaa 
      atccctatt gaagcactg cagactgct ttagtgtat 
      ttaggacag actgatgtt gatttggat gaaacagaa 
      tgtattgtt gctaatctt atttatgaa gggaaaatt 
      aaaggatac atttcgcat caacatagg aaggttgtt 
      gttagtaaa cagaatcca tttccgcca cttacaagt 
      ctatcataa
    mRNA sequence
    • >TC000849 RA
      tgcaaagac ttgtttatt ttcaagctt attcaaaca 
      ttttttcgt caaactcgt aagacataa tgttctttg 
      cgtaacgga agcaacaaa tataacact tgtgcctaa 
      atcaccaaa atgactttt atgtccgtt gctcattac 
      ctacaactt gtggaaagg tattggcgg tctcaggac 
      ggtgcaaaa gtggccagt tttctgtct cttcgccac 
      gaacatgca ggttatgcc aattaccac attgagagt 
      cctgaggga atagttgag cgttttttg accgcccct 
      atcgacgaa ttagtgatt ttgcaccta aaatgcctg 
      tttgccata agccagagt gactatcta tcggcttat 
      aacattcag agcaatttg acgcaaact ttcactaag 
      atcttgcaa tcgcaaaag gaggagaat tggtctttg 
      cctattatg tatgtcttg tgcttagac ttgcgtctt 
      ttggcgcaa ttagccgag aatcagagg agcggaatt 
      aatgagaag cccggcgaa atgctcgag aaggcagca 
      gaatgcttg atgggttgt ttcagggtg tgtgctgct 
      gataatagg tcagcagag gaggataca aagaggata 
      gggatgttg gctttggtg aatcaattg tttaaggtt 
      tattttcgg ataaataag ctacatttg tgcaagccc 
      ttaatcaga gcgattgag tcatcaccg tttaaagag 
      agtttttct ctgagccaa caaatcaca tatcggtat 
      tttgtgggg cgcaaggct atgtttgat agcgattat 
      aaagtcgcc gatgagtat ttaacttat gcttttgaa 
      aattgtcac aagctcagt aagaagaac aaaacgctt 
      atattgatt tacttagtt cctgtgaaa atgttgttg 
      gggtatatc ccaagcaag gaggttctg gagaagtat 
      gacgtcatc cagttgtgg gatttagta caagctgta 
      tgtcagggt aatctcaaa gcgtttgac gagattatg 
      gaaaagcac gaaaccttt ttcatcaag tgtggaata 
      tatctgatt gttgataaa ctgaaaatc atagcatat 
      agaaatttg tttaaaaag gtgtatttg attctaaat 
      acgcatcaa atccctatt gaagcactg cagactgct 
      ttagtgtat ttaggacag actgatgtt gatttggat 
      gaaacagaa tgtattgtt gctaatctt atttatgaa 
      gggaaaatt aaaggatac atttcgcat caacatagg 
      aaggttgtt gttagtaaa cagaatcca tttccgcca 
      cttacaagt ctatcataa ttaagtttt tataagttt 
      gtataattc gtcgttctc tacctgcat tgtaactat 
      tttgtaatt gcatatgaa ataaactgt taaaactac 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY