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Gene information
General information
TC000832 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6438897..6455865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000832
    iBeetle screen iB_03086

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000832 RA
      MGCGHSKIN IYPRKSRNK TNNKKSATP EKTESEEED 
      GVENDLENN ENIEDCESR KTIKVKPFG GPLLAQAEI 
      STSQQDFFK MLDEKIQNG PDYNSESES EKAAEAARL 
      SALLKDWET ASAGSRSLP ATPKRRPKP PLAEKIRSD 
      GVRSAEIAR DGPRPPERG YVSQIPQSV IHQSYGVTQ 
      VPYRHVNYP PPSYITQNP PQQISYPSA MQYQAISPM 
      YTSQPTYQP PPPRQYPSY NQHGSPIKT YMNNSPKHI 
      PYDQPAYSP SHKLYGPPV AKPVYSNGE VLQQQFYTQ 
      MQYQQYKEA RDQVLQNQQ PAGYRMQRS YQSSVTIPI 
      VVQNQQNDM NALHRKQYE LT
    CDS sequence
    • >TC000832 RA
      atgggctgt ggccacagc aaaatcaac atttatccc 
      aggaagtct agaaataaa acgaataac aaaaagtca 
      gcaacacct gaaaaaacc gagtcggag gaagaggac 
      ggggtggaa aacgatctg gaaaataac gaaaatatc 
      gaagactgc gaatcaaga aaaacaatc aaagtgaag 
      ccctttggt ggacctctc ttagctcaa gcagaaatt 
      tcaacaagt cagcaggat tttttcaaa atgctggac 
      gagaaaatc caaaatggt cccgactac aactcggag 
      agtgagtct gaaaaagcg gccgaggcg gccagatta 
      agtgcgctt ctcaaggac tgggaaaca gcgagtgcc 
      ggctctagg tccctgcca gccaccccc aaacgccgc 
      cccaagccg ccgcttgca gagaaaatt cgttctgat 
      ggcgtgcga tctgctgaa atcgccaga gacggacct 
      cgaccgccg gaaaggggc tacgtctca cagatacca 
      caaagtgtg attcatcaa tcgtacggt gtaacgcaa 
      gtaccgtat agacatgta aattaccca ccccctagc 
      tacatcacc cagaaccct ccccaacaa atctcctac 
      ccatctgct atgcaatac caagccatt agtccaatg 
      tacacgagc caacccacc taccaacct cccccgcct 
      cggcaatac cccagctat aaccagcat gggagcccc 
      atcaaaacc tacatgaac aattcacct aaacacatt 
      ccttacgac caaccagct tatagtcct agtcataag 
      ctctacggc ccgcctgtt gccaagcca gtctacagc 
      aatggagag gtgttgcaa cagcagttc tacacccag 
      atgcagtac cagcagtac aaggaagca agggatcaa 
      gttttacag aaccaacag cctgcaggc tacagaatg 
      cagaggagt taccaaagc agtgtcacc atacccata 
      gtagtgcaa aaccagcaa aacgacatg aatgcgcta 
      caccggaag cagtacgaa ctcacgtga
    mRNA sequence
    • >TC000832 RA
      ccatagtaa aacaggtaa acaattaaa aactaatct 
      ataaactga tcgggtttt gcgactcca gtgccaatt 
      cacacgtga gcgtgcgtc taacacgcg atcgcccaa 
      tttttgtaa atattttca tatcggggc tttttggaa 
      cgatttcaa ttattaaaa atatgcaaa catcgctgt 
      aaacatatt tatttttca aagtatttt aaaacatgt 
      gagctagct gtgcatgga ggcctaact tgtgtgagg 
      atttcgagg tttattttt ttccaccta ttgaattga 
      aaaatagtg cgtgattgg cgtatcaga tgttatcag 
      gatttgtaa gatggaagc ttcactgaa aattcacgg 
      tcatctggc agctaatgg tgcagatat tggttgata 
      aataaaatc aggtcataa gaaaatgtg atcgtctac 
      cgaactaag agctgcggt ggtattact ctgccctct 
      gcacccatc tagaaatgg gctgtggcc acagcaaaa 
      tcaacattt atcccagga agtctagaa ataaaacga 
      ataacaaaa agtcagcaa cacctgaaa aaaccgagt 
      cggaggaag aggacgggg tggaaaacg atctggaaa 
      ataacgaaa atatcgaag actgcgaat caagaaaaa 
      caatcaaag tgaagccct ttggtggac ctctcttag 
      ctcaagcag aaatttcaa caagtcagc aggattttt 
      tcaaaatgc tggacgaga aaatccaaa atggtcccg 
      actacaact cggagagtg agtctgaaa aagcggccg 
      aggcggcca gattaagtg cgcttctca aggactggg 
      aaacagcga gtgccggct ctaggtccc tgccagcca 
      cccccaaac gccgcccca agccgccgc ttgcagaga 
      aaattcgtt ctgatggcg tgcgatctg ctgaaatcg 
      ccagagacg gacctcgac cgccggaaa ggggctacg 
      tctcacaga taccacaaa gtgtgattc atcaatcgt 
      acggtgtaa cgcaagtac cgtatagac atgtaaatt 
      acccacccc ctagctaca tcacccaga accctcccc 
      aacaaatct cctacccat ctgctatgc aataccaag 
      ccattagtc caatgtaca cgagccaac ccacctacc 
      aacctcccc cgcctcggc aatacccca gctataacc 
      agcatggga gccccatca aaacctaca tgaacaatt 
      cacctaaac acattcctt acgaccaac cagcttata 
      gtcctagtc ataagctct acggcccgc ctgttgcca 
      agccagtct acagcaatg gagaggtgt tgcaacagc 
      agttctaca cccagatgc agtaccagc agtacaagg 
      aagcaaggg atcaagttt tacagaacc aacagcctg 
      caggctaca gaatgcaga ggagttacc aaagcagtg 
      tcaccatac ccatagtag tgcaaaacc agcaaaacg 
      acatgaatg cgctacacc ggaagcagt acgaactca 
      cgtgatcat atctgtaaa ttttaggac ttgctgttt 
      tgtgtacct cttctagaa ccaagaaca aatatcttg 
      gtattttgg acaatacaa tcctgaacc ctgatttgt 
      tttctaaat aaagattgt aaattaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY