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Gene information
General information
TC000829 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6355079..6366051
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000829
    iBeetle screen iB_03084

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Head and prothorax not present, potentially due to defects concerned dorsal closure.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: Head and prothorax not present, potentially due to defects concerned dorsal closure.
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000829 RA
      MSIYQLVAV SLAFLGSIL ALNSPCPDL FHYDSTDEP 
      NQWSGTVTL LSDDELQGV WARLIFDEK LTELELHDN 
      FEVVRADNQ REYLIKNRD FVLKPATPV DVKITVKYE 
      GDKIPTLIG YRLNAKMVC PPSNVNSGQ FFPGDLNTK 
      DFLSSITSS SKVSAYACG TTPLSANHE RANKKAQNG 
      QWPWQGAIY IRNNSVEKY VCGATLISN RHVLTPGHC 
      VTYLKSEFV VPARLLRVY LGKYSLDND GENKEMQGF 
      GVERIHLYP GYRSKGLLN DLAVLRLDE PVIISDYVR 
      PVCLSPQDE KRDGLLTGY EIKDEGLQS LGQAEVEVL 
      NEQECIQKN EFLKGFYTD NVFCTSYAE NDSVCVGDS 
      GSSLVVFRG GPKPVWELK GVVTVGVGL QNKYVCNRA 
      STVLLVDVA NYLKWIQEV AS
    CDS sequence
    • >TC000829 RA
      atgtccatt tatcagctt gtggctgtt tcgttggcc 
      ttcttggga tcgatttta gctttaaac tcgccatgt 
      cctgatctc ttccattac gattctaca gatgagcca 
      aatcaatgg agtggaacc gtgacgctg ctgtcagat 
      gatgaactt caaggcgtc tgggctcga cttatcttt 
      gatgaaaag ttaacagag ctcgagctc catgacaat 
      ttcgaagtg gtgagggct gataaccag cgcgaatat 
      ttaattaaa aacagagac tttgtgttg aaacctgca 
      acaccagtg gatgtcaaa ataaccgtt aaatacgag 
      ggggataaa attcccacc ttaattggc tatcgattg 
      aacgccaaa atggtctgt cccccatcg aatgtcaat 
      tctggccaa ttcttcccc ggagactta aacactaaa 
      gattttttg tcttctatc acctcaagc tcgaaagtt 
      tccgcttac gcttgtgga actactccc ctgtcggca 
      aatcatgaa cgtgcaaat aaaaaggcg caaaatgga 
      caatggcca tggcaagga gctatttac attcgcaat 
      aacagcgtt gaaaaatac gtctgtgga gccactttg 
      atatcaaac agacacgtt ttaactcct ggccattgt 
      gtcacttat ctcaaatca gaatttgta gtgcctgct 
      cgtctgctg agggtttat cttgggaaa tatagcctt 
      gataacgat ggagagaat aaggaaatg cagggattc 
      ggagtggaa agaatacac ttgtatccg gggtatcgt 
      tcgaagggg ttattaaac gatttggcc gttcttcgc 
      ttggatgaa ccggtgatt atcagtgat tatgttaga 
      ccggtttgc ctaagccct caggatgaa aaacgggac 
      ggacttcta accggatac gaaattaaa gacgaggga 
      ttgcaaagt cttggccag gccgaagtt gaagtcctc 
      aacgagcag gaatgcatc cagaaaaac gagtttttg 
      aaagggttt tacacagat aatgttttt tgcaccagt 
      tacgctgaa aacgattcc gtctgtgtt ggagattct 
      ggaagtagt ttggtggtc tttagaggt ggtccaaag 
      ccagtctgg gagttgaaa ggcgtcgtc accgtcggc 
      gtcggactc caaaataaa tatgtatgc aatcgggcg 
      agtactgtc ctccttgta gacgttgcc aactacttg 
      aaatggatc caagaagtc gcctcttag
    mRNA sequence
    • >TC000829 RA
      gtacagcag aggtgtcat ttattcagt gctgctcaa 
      gtgacttgt gatgtccat ttatcagct tgtggctgt 
      ttcgttggc cttcttggg atcgatttt agctttaaa 
      ctcgccatg tcctgatct cttccatta cgattctac 
      agatgagcc aaatcaatg gagtggaac cgtgacgct 
      gctgtcaga tgatgaact tcaaggcgt ctgggctcg 
      acttatctt tgatgaaaa gttaacaga gctcgagct 
      ccatgacaa tttcgaagt ggtgagggc tgataacca 
      gcgcgaata tttaattaa aaacagaga ctttgtgtt 
      gaaacctgc aacaccagt ggatgtcaa aataaccgt 
      taaatacga gggggataa aattcccac cttaattgg 
      ctatcgatt gaacgccaa aatggtctg tcccccatc 
      gaatgtcaa ttctggcca attcttccc cggagactt 
      aaacactaa agatttttt gtcttctat cacctcaag 
      ctcgaaagt ttccgctta cgcttgtgg aactactcc 
      cctgtcggc aaatcatga acgtgcaaa taaaaaggc 
      gcaaaatgg acaatggcc atggcaagg agctattta 
      cattcgcaa taacagcgt tgaaaaata cgtctgtgg 
      agccacttt gatatcaaa cagacacgt tttaactcc 
      tggccattg tgtcactta tctcaaatc agaatttgt 
      agtgcctgc tcgtctgct gagggttta tcttgggaa 
      atatagcct tgataacga tggagagaa taaggaaat 
      gcagggatt cggagtgga aagaataca cttgtatcc 
      ggggtatcg ttcgaaggg gttattaaa cgatttggc 
      cgttcttcg cttggatga accggtgat tatcagtga 
      ttatgttag accggtttg cctaagccc tcaggatga 
      aaaacggga cggacttct aaccggata cgaaattaa 
      agacgaggg attgcaaag tcttggcca ggccgaagt 
      tgaagtcct caacgagca ggaatgcat ccagaaaaa 
      cgagttttt gaaagggtt ttacacaga taatgtttt 
      ttgcaccag ttacgctga aaacgattc cgtctgtgt 
      tggagattc tggaagtag tttggtggt ctttagagg 
      tggtccaaa gccagtctg ggagttgaa aggcgtcgt 
      caccgtcgg cgtcggact ccaaaataa atatgtatg 
      caatcgggc gagtactgt cctccttgt agacgttgc 
      caactactt gaaatggat ccaagaagt cgcctctta 
      gaaatattc acttacttt tatggcctt gtttcagtt 
      tattgtttc tgcatgtaa taaaagcta tttgaacgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY