Status
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Gene information
General information
TC000827 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6304362..6338003
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000827
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03083

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000827 RA
      MCDTTNAST QSIADYLAQ LLKDRKQLA AFPNVFIHV 
      ERLLDEEIA KVRASLFQI NGVKKEPLV LPEADGPVT 
      TLTEKVYVP VKEHPDFNF VGRILGPRG MTAKQLEQE 
      TGCKIMVRG KGSMRDKKK EDQNRGKPN WEHLSDDLH 
      VLLTVEDTE NRAQIKLQR AVEEVKKLL VPQADGEDE 
      LKKRQLMEL AIINGTYRD SSSKAVSAT ACDEEWRRV 
      AAAAAETQR LLSPAIPGL ATPLRTPAT PLGAPLILS 
      PRSLLSPGD PHGLIYTPY ADYTNYAAL AASPLLTEY 
      ATADHSGGL FAR
    • >TC000827 RB
      MCDTTNAST QSIADYLAQ LLKDRKQLA AFPNVFIHV 
      ERLLDEEIA KVRASLFQI NGVKKEPLV LPEADGPVT 
      TLTEKVYVP VKEHPDFNF VGRILGPRG MTAKQLEQE 
      TGCKIMVRG KGSMRDKKK EDQNRGKPN WEHLSDDLH 
      VLLTVEDTE NRAQIKLQR AVEEVKKLL VPQADGEDE 
      LKKRQLMEL AIINGTYRD SSSKAVSAT ACDEEWRRV 
      AAAAAETQR LLSPAIPGL ATPLRTPAT PLGAPLILS 
      PRMSVPTTA ASILNGSAP PGSLLSPGD PHGLIYTPY 
      ADYTNYAAL AASPLLTEY ATADHSGGL FAR
    • >TC000827 RC
      MCDTTNAST QSIADYLAQ LLKDRKQLA AFPNVFIHV 
      ERLLDEEIA KVRASLFQI NGVKKEPLV LPEADGPVT 
      TLTEKVYVP VKEHPDFNF VGRILGPRG MTAKQLEQE 
      TGCKIMVRG KGSMRDKKK EDQNRGKPN WEHLSDDLH 
      VLLTVEDTE NRAQIKLQR AVEEVKKLL VPQADGEDE 
      LKKRQLMEL AIINGTYRD SSSKAVSAT ACDEEWRRV 
      AAAAAETQR LLSPAIPGL ATPLRTPAT PLGAPLILS 
      PRMSVPTTA ASILNGSAP PGSLLSPGD PHGLIYTPY 
      ADYTNYAAL AASPLLTEY ATADHSGAA AVAAKQRRH 
      LGQIREHPY QRAGALS
    CDS sequence
    • >TC000827 RA
      atgtgtgat acaacgaat gcctcgacg cagagcata 
      gcggactat ttagcacag ttactgaaa gataggaaa 
      caactagca gcctttccc aatgtgttt attcacgtt 
      gagcgtctc ttggacgaa gaaatagcg aaggttcgt 
      gctagtctg tttcaaata aatggagtc aagaaagaa 
      cctctcgta ttgccagaa gctgacgga cccgttaca 
      acgctcaca gaaaaagta tatgtgcca gtcaaagaa 
      catccagat tttaatttc gtgggaagg attctgggc 
      ccccgtgga atgacagct aaacagtta gaacaagaa 
      acaggctgc aaaataatg gtcagaggg aagggctca 
      atgagagac aaaaagaag gaggatcaa aacagagga 
      aaacccaat tgggagcac ctttcagac gatcttcac 
      gtactttta acggtggaa gacaccgaa aatagagca 
      caaatcaag ctgcaaagg gcagtggaa gaagtcaaa 
      aaattgctc gtcccacaa gctgatgga gaggacgag 
      ttgaaaaaa agacagttg atggagctt gccataata 
      aacggaacg tatcgggat tctagttct aaggccgtt 
      tcagcaaca gcttgcgac gaagaatgg cggcgtgtg 
      gcggcggcg gcggccgaa acgcaacga ttgctctcg 
      ccggccatc cccggccta gccaccccc cttcgaacc 
      cctgccacc ccactcggt gctcccctc atactttca 
      ccccgttcc ctcttgtcc cccggggat ccccacggc 
      ctcatatac acaccctat gcggactac acgaattac 
      gccgccttg gcagcgtct cccctacta acggaatac 
      gcaacggcc gatcattct ggtgggtta ttcgctcgc 
      tga
    • >TC000827 RB
      atgtgtgat acaacgaat gcctcgacg cagagcata 
      gcggactat ttagcacag ttactgaaa gataggaaa 
      caactagca gcctttccc aatgtgttt attcacgtt 
      gagcgtctc ttggacgaa gaaatagcg aaggttcgt 
      gctagtctg tttcaaata aatggagtc aagaaagaa 
      cctctcgta ttgccagaa gctgacgga cccgttaca 
      acgctcaca gaaaaagta tatgtgcca gtcaaagaa 
      catccagat tttaatttc gtgggaagg attctgggc 
      ccccgtgga atgacagct aaacagtta gaacaagaa 
      acaggctgc aaaataatg gtcagaggg aagggctca 
      atgagagac aaaaagaag gaggatcaa aacagagga 
      aaacccaat tgggagcac ctttcagac gatcttcac 
      gtactttta acggtggaa gacaccgaa aatagagca 
      caaatcaag ctgcaaagg gcagtggaa gaagtcaaa 
      aaattgctc gtcccacaa gctgatgga gaggacgag 
      ttgaaaaaa agacagttg atggagctt gccataata 
      aacggaacg tatcgggat tctagttct aaggccgtt 
      tcagcaaca gcttgcgac gaagaatgg cggcgtgtg 
      gcggcggcg gcggccgaa acgcaacga ttgctctcg 
      ccggccatc cccggccta gccaccccc cttcgaacc 
      cctgccacc ccactcggt gctcccctc atactttca 
      ccccgtatg tcagtacca acaacggca gcatccatt 
      cttaatggt tctgcccct ccaggttcc ctcttgtcc 
      cccggggat ccccacggc ctcatatac acaccctat 
      gcggactac acgaattac gccgccttg gcagcgtct 
      cccctacta acggaatac gcaacggcc gatcattct 
      ggtgggtta ttcgctcgc tga
    • >TC000827 RC
      atgtgtgat acaacgaat gcctcgacg cagagcata 
      gcggactat ttagcacag ttactgaaa gataggaaa 
      caactagca gcctttccc aatgtgttt attcacgtt 
      gagcgtctc ttggacgaa gaaatagcg aaggttcgt 
      gctagtctg tttcaaata aatggagtc aagaaagaa 
      cctctcgta ttgccagaa gctgacgga cccgttaca 
      acgctcaca gaaaaagta tatgtgcca gtcaaagaa 
      catccagat tttaatttc gtgggaagg attctgggc 
      ccccgtgga atgacagct aaacagtta gaacaagaa 
      acaggctgc aaaataatg gtcagaggg aagggctca 
      atgagagac aaaaagaag gaggatcaa aacagagga 
      aaacccaat tgggagcac ctttcagac gatcttcac 
      gtactttta acggtggaa gacaccgaa aatagagca 
      caaatcaag ctgcaaagg gcagtggaa gaagtcaaa 
      aaattgctc gtcccacaa gctgatgga gaggacgag 
      ttgaaaaaa agacagttg atggagctt gccataata 
      aacggaacg tatcgggat tctagttct aaggccgtt 
      tcagcaaca gcttgcgac gaagaatgg cggcgtgtg 
      gcggcggcg gcggccgaa acgcaacga ttgctctcg 
      ccggccatc cccggccta gccaccccc cttcgaacc 
      cctgccacc ccactcggt gctcccctc atactttca 
      ccccgtatg tcagtacca acaacggca gcatccatt 
      cttaatggt tctgcccct ccaggttcc ctcttgtcc 
      cccggggat ccccacggc ctcatatac acaccctat 
      gcggactac acgaattac gccgccttg gcagcgtct 
      cccctacta acggaatac gcaacggcc gatcattct 
      ggtgcagcg gcagtagct gcgaaacag cgcaggcat 
      ttggggcaa ataagggag catccttac caaagagct 
      ggtgctctg tcataa
    mRNA sequence
    • >TC000827 RA
      taccaccta gttgctctt tctgtgctt aactcagtc 
      gaattttgt agtgcgtcg agaaagttc gtgttaaaa 
      ctccatgaa atcacccca gtaaacaaa agttagcaa 
      acaacgtgt gggacattt tgttataca aaatgtgtg 
      atacaacga atgcctcga cgcagagca tagcggact 
      atttagcac agttactga aagatagga aacaactag 
      cagcctttc ccaatgtgt ttattcacg ttgagcgtc 
      tcttggacg aagaaatag cgaaggttc gtgctagtc 
      tgtttcaaa taaatggag tcaagaaag aacctctcg 
      tattgccag aagctgacg gacccgtta caacgctca 
      cagaaaaag tatatgtgc cagtcaaag aacatccag 
      attttaatt tcgtgggaa ggattctgg gcccccgtg 
      gaatgacag ctaaacagt tagaacaag aaacaggct 
      gcaaaataa tggtcagag ggaagggct caatgagag 
      acaaaaaga aggaggatc aaaacagag gaaaaccca 
      attgggagc acctttcag acgatcttc acgtacttt 
      taacggtgg aagacaccg aaaatagag cacaaatca 
      agctgcaaa gggcagtgg aagaagtca aaaaattgc 
      tcgtcccac aagctgatg gagaggacg agttgaaaa 
      aaagacagt tgatggagc ttgccataa taaacggaa 
      cgtatcggg attctagtt ctaaggccg tttcagcaa 
      cagcttgcg acgaagaat ggcggcgtg tggcggcgg 
      cggcggccg aaacgcaac gattgctct cgccggcca 
      tccccggcc tagccaccc cccttcgaa cccctgcca 
      ccccactcg gtgctcccc tcatacttt caccccgtt 
      ccctcttgt cccccgggg atccccacg gcctcatat 
      acacaccct atgcggact acacgaatt acgccgcct 
      tggcagcgt ctcccctac taacggaat acgcaacgg 
      ccgatcatt ctggtgggt tattcgctc gctgatgtg 
      cgattttta gatattgtc tagtactta gattgtatt 
      aattgtcat tgtctgtta agggattat tattcatac 
      tattattaa gaaacgcca tcgaactgg ttcggaaaa 
      taacgccta gattttttt tttgatgcg gttttctga 
      agcattttt gggttcgat ggctcgttt tagtcacaa 
      tttatttta attacaatg tttctgtga tacttgccg 
      cgacagccg gttgttacg tgtactcaa agtacgtaa 
      gtaacattg aaaattatt taatttttt tgtattgca 
      agtcatagc atctaagat tatttttta ttttattat 
      tgagactat gtacgtcaa acgtactct tctggtcaa 
      gcaaacaat agtcaaatg tttttttta tataagtta 
      tttacagta aaagctaaa gtaaacaca gatcgttat 
      aatacaatt ttatgtaca tatttttta attttatac 
      aaatatttt aaaaatgat ttatagtgt agtgtgtat 
      ggtaaaagc atttattac gttacctta agctaatcg 
      cagtaagtg tacataaaa cacgtttga tattattac 
      agtggggtc cggttataa cgaactaaa tacacgaaa 
      taaataggt acctagtgt aaaaaaacg tgaattatt 
      tgcaaacaa tagggctag gggtgatag tagagctca 
      aaagtgaaa aataactca acttacctt atccgaata 
      tttatattt ctgagatat agagagttt tggtcggcg 
      aataaattt ggcaccaac aaaaatttt aaactgatc 
      tgtgagctt agttcttta aaatctcaa aattcggtg 
      aaaagttcg tcataaacg gaaaattgc atcacattt 
      taagaatct agttcgctg tagccggta aaaaatgca 
      atgagctta tggaaagat tttcggtgc ttgtcaaat 
      cgtacgtta taagcggac tccactgta tttcagtcc 
      ttgacaaag tatccgaaa atgaacaat ttatttatt 
      tatttctgt tacgttcgt aacaacaaa actcgttca 
      gtgctttaa ttgttttag tagctacaa agtagtgtt 
      agaattttt tatatttat gttagagag tgactgcat 
      caaaatcac agctgcatc tataataat cgttgaggc 
      atttaatcg cgttgtgtt catgagctg cgttataga 
      tgttgttga tttggtgtt agcatcgac tccgtcacc 
      tttcaggag actgcttta atttcatgt actttaaat 
      gctgatttg tataggtgc agcggcagt agctgcgaa 
      acagcgcag gcatttggg gcaaataag ggagcatcc 
      ttaccaaag agctggtgc tctgtcata aacctaaaa 
      ggttagtta cgttttgtt ttctatttc tttcagatg 
      tttgccagt catgtgtgt ctcagtgtg gagttatga 
      tgtatctat tgtagtatt tcacctcgt cagcttatt 
      gtttttatt ttattcgat gatatcgtc agtattgag 
      tgaatttca tgcgggttg tgacactac atacgttca 
      gttgcaaat ttggggaag gaggccttt tgggttacc 
      acttgtacc tcagtgaat aactagtcg aaaatttca 
      aaccacctt cttcccggt gtcaaaatc agtttcagt 
      ttcattttt acgtatgtc ttattgtta gtacgcgga 
      gctgtcgct tcgcgctcc atgaatatg tttaagtag 
      ttttataca tatacgttt ataaatagc atattatat 
      tttttgtat gtttatgtt gttgacttg cgccaatgt 
      ggaacaaaa tatatttat ataaaaatc
    • >TC000827 RB
      taccaccta gttgctctt tctgtgctt aactcagtc 
      gaattttgt agtgcgtcg agaaagttc gtgttaaaa 
      ctccatgaa atcacccca gtaaacaaa agttagcaa 
      acaacgtgt gggacattt tgttataca aaatgtgtg 
      atacaacga atgcctcga cgcagagca tagcggact 
      atttagcac agttactga aagatagga aacaactag 
      cagcctttc ccaatgtgt ttattcacg ttgagcgtc 
      tcttggacg aagaaatag cgaaggttc gtgctagtc 
      tgtttcaaa taaatggag tcaagaaag aacctctcg 
      tattgccag aagctgacg gacccgtta caacgctca 
      cagaaaaag tatatgtgc cagtcaaag aacatccag 
      attttaatt tcgtgggaa ggattctgg gcccccgtg 
      gaatgacag ctaaacagt tagaacaag aaacaggct 
      gcaaaataa tggtcagag ggaagggct caatgagag 
      acaaaaaga aggaggatc aaaacagag gaaaaccca 
      attgggagc acctttcag acgatcttc acgtacttt 
      taacggtgg aagacaccg aaaatagag cacaaatca 
      agctgcaaa gggcagtgg aagaagtca aaaaattgc 
      tcgtcccac aagctgatg gagaggacg agttgaaaa 
      aaagacagt tgatggagc ttgccataa taaacggaa 
      cgtatcggg attctagtt ctaaggccg tttcagcaa 
      cagcttgcg acgaagaat ggcggcgtg tggcggcgg 
      cggcggccg aaacgcaac gattgctct cgccggcca 
      tccccggcc tagccaccc cccttcgaa cccctgcca 
      ccccactcg gtgctcccc tcatacttt caccccgta 
      tgtcagtac caacaacgg cagcatcca ttcttaatg 
      gttctgccc ctccaggtt ccctcttgt cccccgggg 
      atccccacg gcctcatat acacaccct atgcggact 
      acacgaatt acgccgcct tggcagcgt ctcccctac 
      taacggaat acgcaacgg ccgatcatt ctggtgggt 
      tattcgctc gctgatgtg cgattttta gatattgtc 
      tagtactta gattgtatt aattgtcat tgtctgtta 
      agggattat tattcatac tattattaa gaaacgcca 
      tcgaactgg ttcggaaaa taacgccta gattttttt 
      tttgatgcg gttttctga agcattttt gggttcgat 
      ggctcgttt tagtcacaa tttatttta attacaatg 
      tttctgtga tacttgccg cgacagccg gttgttacg 
      tgtactcaa agtacgtaa gtaacattg aaaattatt 
      taatttttt tgtattgca agtcatagc atctaagat 
      tatttttta ttttattat tgagactat gtacgtcaa 
      acgtactct tctggtcaa gcaaacaat agtcaaatg 
      tttttttta tataagtta tttacagta aaagctaaa 
      gtaaacaca gatcgttat aatacaatt ttatgtaca 
      tatttttta attttatac aaatatttt aaaaatgat 
      ttatagtgt agtgtgtat ggtaaaagc atttattac 
      gttacctta agctaatcg cagtaagtg tacataaaa 
      cacgtttga tattattac agtggggtc cggttataa 
      cgaactaaa tacacgaaa taaataggt acctagtgt 
      aaaaaaacg tgaattatt tgcaaacaa tagggctag 
      gggtgatag tagagctca aaagtgaaa aataactca 
      acttacctt atccgaata tttatattt ctgagatat 
      agagagttt tggtcggcg aataaattt ggcaccaac 
      aaaaatttt aaactgatc tgtgagctt agttcttta 
      aaatctcaa aattcggtg aaaagttcg tcataaacg 
      gaaaattgc atcacattt taagaatct agttcgctg 
      tagccggta aaaaatgca atgagctta tggaaagat 
      tttcggtgc ttgtcaaat cgtacgtta taagcggac 
      tccactgta tttcagtcc ttgacaaag tatccgaaa 
      atgaacaat ttatttatt tatttctgt tacgttcgt 
      aacaacaaa actcgttca gtgctttaa ttgttttag 
      tagctacaa agtagtgtt agaattttt tatatttat 
      gttagagag tgactgcat caaaatcac agctgcatc 
      tataataat cgttgaggc atttaatcg cgttgtgtt 
      catgagctg cgttataga tgttgttga tttggtgtt 
      agcatcgac tccgtcacc tttcaggag actgcttta 
      atttcatgt actttaaat gctgatttg tataggtgc 
      agcggcagt agctgcgaa acagcgcag gcatttggg 
      gcaaataag ggagcatcc ttaccaaag agctggtgc 
      tctgtcata aacctaaaa ggttagtta cgttttgtt 
      ttctatttc tttcagatg tttgccagt catgtgtgt 
      ctcagtgtg gagttatga tgtatctat tgtagtatt 
      tcacctcgt cagcttatt gtttttatt ttattcgat 
      gatatcgtc agtattgag tgaatttca tgcgggttg 
      tgacactac atacgttca gttgcaaat ttggggaag 
      gaggccttt tgggttacc acttgtacc tcagtgaat 
      aactagtcg aaaatttca aaccacctt cttcccggt 
      gtcaaaatc agtttcagt ttcattttt acgtatgtc 
      ttattgtta gtacgcgga gctgtcgct tcgcgctcc 
      atgaatatg tttaagtag ttttataca tatacgttt 
      ataaatagc atattatat tttttgtat gtttatgtt 
      gttgacttg cgccaatgt ggaacaaaa tatatttat 
      ataaaaatc
    • >TC000827 RC
      taccaccta gttgctctt tctgtgctt aactcagtc 
      gaattttgt agtgcgtcg agaaagttc gtgttaaaa 
      ctccatgaa atcacccca gtaaacaaa agttagcaa 
      acaacgtgt gggacattt tgttataca aaatgtgtg 
      atacaacga atgcctcga cgcagagca tagcggact 
      atttagcac agttactga aagatagga aacaactag 
      cagcctttc ccaatgtgt ttattcacg ttgagcgtc 
      tcttggacg aagaaatag cgaaggttc gtgctagtc 
      tgtttcaaa taaatggag tcaagaaag aacctctcg 
      tattgccag aagctgacg gacccgtta caacgctca 
      cagaaaaag tatatgtgc cagtcaaag aacatccag 
      attttaatt tcgtgggaa ggattctgg gcccccgtg 
      gaatgacag ctaaacagt tagaacaag aaacaggct 
      gcaaaataa tggtcagag ggaagggct caatgagag 
      acaaaaaga aggaggatc aaaacagag gaaaaccca 
      attgggagc acctttcag acgatcttc acgtacttt 
      taacggtgg aagacaccg aaaatagag cacaaatca 
      agctgcaaa gggcagtgg aagaagtca aaaaattgc 
      tcgtcccac aagctgatg gagaggacg agttgaaaa 
      aaagacagt tgatggagc ttgccataa taaacggaa 
      cgtatcggg attctagtt ctaaggccg tttcagcaa 
      cagcttgcg acgaagaat ggcggcgtg tggcggcgg 
      cggcggccg aaacgcaac gattgctct cgccggcca 
      tccccggcc tagccaccc cccttcgaa cccctgcca 
      ccccactcg gtgctcccc tcatacttt caccccgta 
      tgtcagtac caacaacgg cagcatcca ttcttaatg 
      gttctgccc ctccaggtt ccctcttgt cccccgggg 
      atccccacg gcctcatat acacaccct atgcggact 
      acacgaatt acgccgcct tggcagcgt ctcccctac 
      taacggaat acgcaacgg ccgatcatt ctggtgcag 
      cggcagtag ctgcgaaac agcgcaggc atttggggc 
      aaataaggg agcatcctt accaaagag ctggtgctc 
      tgtcataaa cctaaaagg ttagttacg ttttgtttt 
      ctatttctt tcagatgtt tgccagtca tgtgtgtct 
      cagtgtgga gttatgatg tatctattg tagtatttc 
      acctcgtca gcttattgt ttttatttt attcgatga 
      tatcgtcag tattgagtg aatttcatg cgggttgtg 
      acactacat acgttcagt tgcaaattt ggggaagga 
      ggccttttg ggttaccac ttgtacctc agtgaataa 
      ctagtcgaa aatttcaaa ccaccttct tcccggtgt 
      caaaatcag tttcagttt catttttac gtatgtctt 
      attgttagt acgcggagc tgtcgcttc gcgctccat 
      gaatatgtt taagtagtt ttatacata tacgtttat 
      aaatagcat attatattt tttgtatgt ttatgttgt 
      tgacttgcg ccaatgtgg aacaaaata tatttatat 
      aaaaatc
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