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Gene information
General information
TC000817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 707753..709858
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000817
    iBeetle screen iB_03082
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen musculature random irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    App. 50 larvae on slide.

    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000817 RA
      MISSALVWG FLYDTLGRK RLLMVGFFL DTVFVFISG 
      FSQSKTILM ICKFFGGFI INGPFAALT AYISEFHCS 
      KYRARMQLV LGTIFSCGS VTLPVLAWA VLPQNYYFS 
      LFNKTLEFH SWNIYLFIC GFPALFSTV IFIFMPESP 
      KFLMTVGQN DKALKVFQK IYSVNSRNP PETYPIRQL 
      VDEIEVKKN SQSKHTTHV TANRNKIQA LREGWQQLK 
      PLFFPPHLA NIILVCLLQ TLFTMSLNT LRLWLPQIF 
      QAINDYKYY HNDSSADLC KMLDMIQPA STLGECVVN 
      LNNSSVYIN TIIISSVSA SSYIFAGYF INILGKKKL 
      MSVLGGSCA FSIYFSTNT AMTVAFASI FIASSSVAT 
      NVVLAVCID LFPTTLRTM TLSLSMMLA RSGAMIGNL 
      VFPLLLQTG CAPPFFTIG SLIFVCAFI AMLLPNTDL 
      KALE
    CDS sequence
    • >TC000817 RA
      atgatttcg agtgccttg gtttgggga tttctttat 
      gacacttta gggaggaaa agactgttg atggttggc 
      ttcttccta gacactgtt tttgtcttc atcagcggt 
      ttctcccaa agcaaaaca attctaatg atttgtaaa 
      ttttttggc ggctttata ataaacggc ccgtttgct 
      gctctaaca gcctatatt tctgagttc cattgttcc 
      aaatataga gcaagaatg cagctagta cttggaact 
      atttttagt tgtgggagc gttacttta cctgtcttg 
      gcctgggct gtcttgccc caaaattac tacttttca 
      ctttttaac aaaacactt gaatttcat tcctggaac 
      atttatctt ttcatttgt ggctttccc gccctgttc 
      agtaccgta atatttatt ttcatgccc gaaagtccg 
      aaatttctg atgacggtg ggccaaaac gacaaggca 
      ctcaaagtg tttcaaaaa atctacagt gtaaatagc 
      aggaatcca cctgaaacc tatccaatt aggcaacta 
      gttgacgaa attgaagtt aagaaaaac agtcaaagt 
      aagcacaca actcacgtg acagccaac aggaacaag 
      atacaagct ctcagagaa gggtggcaa caactaaaa 
      cccttattt ttcccgcca catttagca aacattatc 
      cttgtgtgt ctcctccaa actttattt acaatgagt 
      ttgaacact ttgcggctt tggttgccg caaattttt 
      caagccatc aatgactac aagtattat cacaacgat 
      tctagtgcc gatctttgc aaaatgctg gacatgatc 
      cagccagcc agtacttta ggcgaatgc gttgttaac 
      ctcaacaat tcctcagtt tatatcaac acaataatt 
      atttcgtct gtgagtgct agtagctac atcttcgcc 
      ggctatttc atcaacatc ctcggaaag aaaaaactg 
      atgagtgtt ttgggagga tcgtgtgct ttttcaatc 
      tacttttcc acgaacaca gcaatgacg gtggctttt 
      gcttcaatt tttatcgca tcgtcaagt gtggcaact 
      aatgtagtt ctagccgtt tgcatagac ttgtttccg 
      actacttta agaacaatg acactatcg ttatcaatg 
      atgttggct cgaagtggg gcaatgatt gggaatttg 
      gtcttcccc ttgttactc caaacagga tgtgctcct 
      ccgttcttc acaatcggt tctctaatt tttgtatgt 
      gctttcatc gcaatgctg ctaccaaac actgaccta 
      aaggccttg gagtaa
    mRNA sequence
    • >TC000817 RA
      tcggtcatg tattcatta aaaatctgt cgtcacaat 
      gaaagaaaa acatagcac ggccaaaaa tatctacgt 
      agttcccat agatgacga aactaaagc aaaagctac 
      agaaccggc tgattttga aacggcaat ttcggccac 
      tggcttcgg atactacaa tttatttct gatccttat 
      ctcaattcc tgcaggatg gtcttctat ctttgaaac 
      gaccacaat gtcgtatgt atttgcagc cgcacagtg 
      tgaccttga cttgagttt agatgataa aggaatgtt 
      aaacgcaat tacttactt aggcacgta acaattaac 
      ataactaat agataacga ctgatcttc aggaatgat 
      ttcgagtgc cttggtttg gggatttct ttatgacac 
      tttagggag gaaaagact gttgatggt tggcttctt 
      cctagacac tgtttttgt cttcatcag cggtttctc 
      ccaaagcaa aacaattct aatgatttg taaattttt 
      tggcggctt tataataaa cggcccgtt tgctgctct 
      aacagccta tatttctga gttccattg ttccaaata 
      tagagcaag aatgcagct agtacttgg aactatttt 
      tagttgtgg gagcgttac tttacctgt cttggcctg 
      ggctgtctt gccccaaaa ttactactt ttcactttt 
      taacaaaac acttgaatt tcattcctg gaacattta 
      tcttttcat ttgtggctt tcccgccct gttcagtac 
      cgtaatatt tattttcat gcccgaaag tccgaaatt 
      tctgatgac ggtgggcca aaacgacaa ggcactcaa 
      agtgtttca aaaaatcta cagtgtaaa tagcaggaa 
      tccacctga aacctatcc aattaggca actagttga 
      cgaaattga agttaagaa aaacagtca aagtaagca 
      cacaactca cgtgacagc caacaggaa caagataca 
      agctctcag agaagggtg gcaacaact aaaaccctt 
      atttttccc gccacattt agcaaacat tatccttgt 
      gtgtctcct ccaaacttt atttacaat gagtttgaa 
      cactttgcg gctttggtt gccgcaaat ttttcaagc 
      catcaatga ctacaagta ttatcacaa cgattctag 
      tgccgatct ttgcaaaat gctggacat gatccagcc 
      agccagtac tttaggcga atgcgttgt taacctcaa 
      caattcctc agtttatat caacacaat aattatttc 
      gtctgtgag tgctagtag ctacatctt cgccggcta 
      tttcatcaa catcctcgg aaagaaaaa actgatgag 
      tgttttggg aggatcgtg tgctttttc aatctactt 
      ttccacgaa cacagcaat gacggtggc ttttgcttc 
      aatttttat cgcatcgtc aagtgtggc aactaatgt 
      agttctagc cgtttgcat agacttgtt tccgactac 
      tttaagaac aatgacact atcgttatc aatgatgtt 
      ggctcgaag tggggcaat gattgggaa tttggtctt 
      ccccttgtt actccaaac aggatgtgc tcctccgtt 
      cttcacaat cggttctct aatttttgt atgtgcttt 
      catcgcaat gctgctacc aaacactga cctaaaggc 
      cttggagta atcttaaag actgtgaaa ctgtgaaag 
      actttttta ttaatacaa aatacagta agat