Status
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Gene information
General information
TC000810 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6104259..6109694
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000810
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03080

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000810 RA
      MGTMEIGSQ EQQSFGQEE RGKRRVERF EHSTLQLVR 
      TPGVNIFKK GLGEFNSLS KWNPLLFYQ PPAHHGEKS 
      PTLSPILNV DTNLDFSDM EASVLHCVN PYDVNPISG 
      TTVVQEESA STGKPIKLE MEEFSDLAP FLPEFSQKT 
      TTSTTDELN TPIFDRKGP FNFMVYENT VIESPQAAD 
      PFKFEYDTI RTGNSVLSS KQINDFSVY ANTVNVQLQ 
      DTNNSEIVN IVQGSEANI LFATSESDQ GNIYIINGN 
      ETPRHVQSE DQISASLFK VEDTTSVIS VKSNENQYS 
      NVSSPCNEY SVNTNTSTE GQYSNVSSP SNDYSRTNL 
      GIETPEDSN SSCATQISK KVPKLKLNL SFNQPMVLN 
      TPDVIDSVV DLETSFNIL DYVNEKDVT TVNIEDIHF 
      EHPSTSREL TAEIENIAP KLRKRKSKR YDDDEDYVP 
      PSKKAGKRE PISSDSESE VEVSLSSKR GRPPRRTSS 
      ISSDHSDAS KYRELRDKN NEASRKSRL KRKMKEMSL 
      QREAEELYE KNVKLKAQV EEYERMVNN FRNNLFKIM 
      LKK
    CDS sequence
    • >TC000810 RA
      atgggcact atggagatc gggagtcaa gaacaacag 
      agttttggc caagaagaa cgaggaaag cgcagagtt 
      gagcgtttt gagcactca actcttcaa ctggtgagg 
      accccagga gtcaacatt tttaaaaaa ggactaggc 
      gaatttaat tccctgtca aaatggaat ccgctgctg 
      ttttatcag ccacctgcc catcatggc gagaaatca 
      cccacacta agcccaata ctgaacgtg gacacaaat 
      ttggacttc tcagacatg gaagcttca gtactccac 
      tgcgtcaat ccgtacgac gtaaacccg ataagtggt 
      acaacggtc gtacaggaa gagagcgcc tcaactgga 
      aaacccatc aagctcgaa atggaagaa ttcagcgat 
      cttgcgccg tttcttccc gagttttcg caaaagaca 
      acgacaagt actacagat gaactaaat acgccgatt 
      tttgatcga aaaggaccg tttaatttt atggtatat 
      gaaaataca gttatagaa tcaccacaa gccgcagat 
      ccttttaag tttgaatat gacacaatc cggacgggt 
      aatagtgta ctttcaagc aagcaaatc aatgacttt 
      tcagtttac gcaaacact gtgaacgtt caactgcaa 
      gacaccaac aatagtgaa atagttaat atcgttcaa 
      gggtctgag gctaatatc ctgttcgca acatctgaa 
      tcagatcag ggaaatatc tacataatt aatggaaac 
      gaaacccca agacatgtg caaagtgaa gatcaaatt 
      agtgcatct ctatttaaa gttgaagat actactagt 
      gttataagt gtaaaaagt aacgagaat cagtactcg 
      aacgtttct tcgccttgc aatgagtat tccgtgaat 
      accaacacg agcactgaa ggacaatac tccaacgta 
      tcatcaccc agtaatgac tattccagg actaaccta 
      ggaattgag acaccggaa gacagcaac agcagctgt 
      gccacgcaa atatcaaag aaggtgccc aagctgaaa 
      ctaaacctc tcattcaac caaccaatg gtacttaac 
      acccctgac gtcattgac tccgtagtc gacctagag 
      acaagcttc aacatcctc gactacgtc aatgaaaag 
      gatgtgaca acggtaaac atagaggat atccatttt 
      gaacacccc tcaacatcc agggaattg accgccgaa 
      atcgagaac atcgcgcca aagcttcgc aaacgcaag 
      agcaaacga tatgacgac gatgaagat tacgtccct 
      ccgtcgaaa aaagccggc aaaagagag ccgatttct 
      tcagattcc gaaagtgaa gtggaagtg tcgcttagt 
      agtaaacga gggcgaccg ccaagacgc acatcatca 
      atcagttcg gatcactct gatgcttca aaatacagg 
      gaattgaga gataagaat aatgaagct tcgcgaaaa 
      tcaagattg aagaggaag atgaaggaa atgtccctg 
      cagagagaa gctgaagag ttgtacgag aagaacgtc 
      aaacttaaa gcacaagtt gaagagtat gaaaggatg 
      gttaataac ttcaggaat aatttgttc aagataatg 
      cttaaaaaa tag
    mRNA sequence
    • >TC000810 RA
      attgaaacg caaattcac gtgaacacc aattttccc 
      acatcctag ccctgttat caaagcttt gaccatcag 
      tggttcaga cgtctttcc tcatttgaa aataaatta 
      aaaagtgtg atcattgtg acaaagccc aattcgcac 
      aaaataata aattgtgag agttttatt ttttgccaa 
      ttgttaagt gtaggtaga taacgacac ctacttgtt 
      ccctttgtt gtattctaa ttcgtttga taactgata 
      agggaaaag ttgccacgt gtagacttt tttcattcc 
      ggaaccccg taatatgtc cgtttctgg cttggatat 
      tatgcacat ttctaaaac gaaccaatc tcaaactat 
      tgggtttaa ttcaaatac gaaaaacat tttccactg 
      tgttattaa aagaaagtg tagtaaaag aggtcatca 
      ttgcacgag aacgaccat gggcactat ggagatcgg 
      gagtcaaga acaacagag ttttggcca agaagaacg 
      aggaaagcg cagagttga gcgttttga gcactcaac 
      tcttcaact ggtgaggac cccaggagt caacatttt 
      taaaaaagg actaggcga atttaattc cctgtcaaa 
      atggaatcc gctgctgtt ttatcagcc acctgccca 
      tcatggcga gaaatcacc cacactaag cccaatact 
      gaacgtgga cacaaattt ggacttctc agacatgga 
      agcttcagt actccactg cgtcaatcc gtacgacgt 
      aaacccgat aagtggtac aacggtcgt acaggaaga 
      gagcgcctc aactggaaa acccatcaa gctcgaaat 
      ggaagaatt cagcgatct tgcgccgtt tcttcccga 
      gttttcgca aaagacaac gacaagtac tacagatga 
      actaaatac gccgatttt tgatcgaaa aggaccgtt 
      taattttat ggtatatga aaatacagt tatagaatc 
      accacaagc cgcagatcc ttttaagtt tgaatatga 
      cacaatccg gacgggtaa tagtgtact ttcaagcaa 
      gcaaatcaa tgacttttc agtttacgc aaacactgt 
      gaacgttca actgcaaga caccaacaa tagtgaaat 
      agttaatat cgttcaagg gtctgaggc taatatcct 
      gttcgcaac atctgaatc agatcaggg aaatatcta 
      cataattaa tggaaacga aaccccaag acatgtgca 
      aagtgaaga tcaaattag tgcatctct atttaaagt 
      tgaagatac tactagtgt tataagtgt aaaaagtaa 
      cgagaatca gtactcgaa cgtttcttc gccttgcaa 
      tgagtattc cgtgaatac caacacgag cactgaagg 
      acaatactc caacgtatc atcacccag taatgacta 
      ttccaggac taacctagg aattgagac accggaaga 
      cagcaacag cagctgtgc cacgcaaat atcaaagaa 
      ggtgcccaa gctgaaact aaacctctc attcaacca 
      accaatggt acttaacac ccctgacgt cattgactc 
      cgtagtcga cctagagac aagcttcaa catcctcga 
      ctacgtcaa tgaaaagga tgtgacaac ggtaaacat 
      agaggatat ccattttga acacccctc aacatccag 
      ggaattgac cgccgaaat cgagaacat cgcgccaaa 
      gcttcgcaa acgcaagag caaacgata tgacgacga 
      tgaagatta cgtccctcc gtcgaaaaa agccggcaa 
      aagagagcc gatttcttc agattccga aagtgaagt 
      ggaagtgtc gcttagtag taaacgagg gcgaccgcc 
      aagacgcac atcatcaat cagttcgga tcactctga 
      tgcttcaaa atacaggga attgagaga taagaataa 
      tgaagcttc gcgaaaatc aagattgaa gaggaagat 
      gaaggaaat gtccctgca gagagaagc tgaagagtt 
      gtacgagaa gaacgtcaa acttaaagc acaagttga 
      agagtatga aaggatggt taataactt caggaataa 
      tttgttcaa gataatgct taaaaaata gtttagtgt 
      gattatttg ttaagttat gctagtgat ttggggttt 
      ctttaggat aagatgtac tttttattt gttgtttct 
      gttttatag aatacactt agattttta caacttgtt 
      tcatgatat cgtattgta ctattgttc ttaccttat 
      atcttaacg ttttcttat attcactcg tttgttaat 
      tggagaaat tttaattaa agaattgga caattt
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