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Gene information
General information
TC000803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 363306..366026
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000803
    iBeetle screen iB_03079
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000803 RA
      MSLPGYLFS FCLVISLVK CENTNHTTG TTIIQIKNG 
      ELRGRILKT VDLNHVYYA YQGIPYAEP PIGHLRFEP 
      PVPKQNWYG VFDATKDGN VCVQGNPAI GSEDCLNLN 
      VYVPEVSKY YKALLPTMV FIYGGGFEA GFATYDLYG 
      PDHLLEKGV VVVTLNYRT GILGFSSTG DLVIPGNNG 
      LKDQVLALK WVKENIEFF GGDPNQVTL FGQSAGSAS 
      VSYHMQSKL SRGLFHRAI LESGVSLTP WAFSRRGPE 
      VLKIVANDL WIDTSNTRE MVNKLRKVD YKLLQEKAA 
      SAVAFQYLK DDPRHGFIF SPVIEPQHD NAFLTQKSH 
      QMLSNGDFA KVPCIVGFN SLEGTFNFG TLFRVWLLR 
      YDLDHTKLL PIDLNAKKE KKDTAADYI KKHYFNGYL 
      IALSDRKLM KYLSDDLFV RPVREFVRL VSQHVPVYF 
      YQFTYEGGL WGFANRTQH GVTHSEELG YLWRSKFSP 
      SESDLLMRK RMTSMWTDF AKTGNPTPK KYFTSSEVI 
      WKTTDSNFT YLDIGDELL LSQHPNKAS MTLWDQIYQ 
      LYGNPPYDT Y
    CDS sequence
    • >TC000803 RA
      atgtcttta ccaggttac ttattctct ttttgcctc 
      gtcatttct ttagtgaaa tgtgaaaat acgaaccac 
      acaaccggc acaacaatt attcaaatc aaaaacggt 
      gaattgaga ggtcgcata cttaaaaca gtggatctt 
      aatcatgtt tattatgcc tatcaagga ataccatac 
      gcggaacca ccaattgga cacctccga tttgaacca 
      cctgtccct aaacaaaac tggtacgga gttttcgac 
      gccaccaaa gacggaaac gtgtgcgtc caaggaaac 
      cctgccata ggttccgaa gattgcctc aacctcaac 
      gtttacgtc cctgaggtt tccaagtat tacaaggcg 
      ttgttgcca acgatggtg tttatctat ggtggtggt 
      ttcgaggcc ggttttgcc acctatgat ctgtacggc 
      cccgaccac ctgctggaa aaaggggtg gttgtggta 
      actcttaat taccgcact gggattttg ggattttca 
      agtacgggc gatttggtg attccgggt aacaacgga 
      ttgaaagac caagtcctg gctctgaaa tgggtcaag 
      gaaaatatc gaatttttt ggcggagat ccaaaccaa 
      gtgactctt tttgggcaa agtgctggg tctgcttct 
      gtttcctac cacatgcaa tcaaaattg tctcgagga 
      ctttttcat agggctatc ctggaatct ggtgtatcc 
      ctcactcct tgggctttc agtcgacga ggtccagaa 
      gtgctaaaa attgtagct aacgatttg tggatagat 
      acgagtaat acgcgagaa atggtcaat aaactgaga 
      aaagtggat tataaactt ttgcaagaa aaagcggca 
      tcagctgtg gctttccaa tatttaaaa gacgatcca 
      cgacacggt tttatattt tcaccagtt attgagccc 
      caacacgac aacgcattc ttgactcaa aaaagccat 
      caaatgttg tctaatggt gactttgcc aaagtccca 
      tgtattgtc ggttttaac tcactagaa ggcactttt 
      aatttcgga accttattt agagtttgg cttttgcgc 
      tacgacctg gaccacaca aaattgtta ccaattgat 
      ttaaacgca aaaaaagaa aagaaagat actgctgca 
      gactacata aagaagcat tatttcaac ggatatctg 
      atcgcactt tctgaccga aaattaatg aagtatttg 
      agtgacgat ttgtttgtt cggccagtt cgtgaattt 
      gtccgattg gtgtcacag cacgttccc gtttacttt 
      taccagttt acatacgag ggtggattg tggggtttt 
      gcgaatagg acgcaacat ggcgtaaca catagtgaa 
      gaattgggg tatttgtgg agatcaaag tttagtcct 
      tccgaatcg gatctgctt atgaggaaa agaatgact 
      tcaatgtgg acagatttt gcaaaaact ggaaatcca 
      acgccaaaa aaatatttc acgtcctct gaagtcatc 
      tggaaaacc accgattct aatttcact tatctcgat 
      atcggtgat gaactatta ctaagtcaa catcctaat 
      aaagctagc atgactttg tgggaccag atctaccaa 
      ctttacgga aatccacct tatgacacg tattaa
    mRNA sequence
    • >TC000803 RA
      ttttactta aatatacaa atcacctga acgacggtt 
      atttgttaa aaatgtctt taccaggtt acttattct 
      ctttttgcc tcgtcattt ctttagtga aatgtgaaa 
      atacgaacc acacaaccg gcacaacaa ttattcaaa 
      tcaaaaacg gtgaattga gaggtcgca tacttaaaa 
      cagtggatc ttaatcatg tttattatg cctatcaag 
      gaataccat acgcggaac caccaattg gacacctcc 
      gatttgaac cacctgtcc ctaaacaaa actggtacg 
      gagttttcg acgccacca aagacggaa acgtgtgcg 
      tccaaggaa accctgcca taggttccg aagattgcc 
      tcaacctca acgtttacg tccctgagg tttccaagt 
      attacaagg cgttgttgc caacgatgg tgtttatct 
      atggtggtg gtttcgagg ccggttttg ccacctatg 
      atctgtacg gccccgacc acctgctgg aaaaagggg 
      tggttgtgg taactctta attaccgca ctgggattt 
      tgggatttt caagtacgg gcgatttgg tgattccgg 
      gtaacaacg gattgaaag accaagtcc tggctctga 
      aatgggtca aggaaaata tcgaatttt ttggcggag 
      atccaaacc aagtgactc tttttgggc aaagtgctg 
      ggtctgctt ctgtttcct accacatgc aatcaaaat 
      tgtctcgag gactttttc atagggcta tcctggaat 
      ctggtgtat ccctcactc cttgggctt tcagtcgac 
      gaggtccag aagtgctaa aaattgtag ctaacgatt 
      tgtggatag atacgagta atacgcgag aaatggtca 
      ataaactga gaaaagtgg attataaac ttttgcaag 
      aaaaagcgg catcagctg tggctttcc aatatttaa 
      aagacgatc cacgacacg gttttatat tttcaccag 
      ttattgagc cccaacacg acaacgcat tcttgactc 
      aaaaaagcc atcaaatgt tgtctaatg gtgactttg 
      ccaaagtcc catgtattg tcggtttta actcactag 
      aaggcactt ttaatttcg gaaccttat ttagagttt 
      ggcttttgc gctacgacc tggaccaca caaaattgt 
      taccaattg atttaaacg caaaaaaag aaaagaaag 
      atactgctg cagactaca taaagaagc attatttca 
      acggatatc tgatcgcac tttctgacc gaaaattaa 
      tgaagtatt tgagtgacg atttgtttg ttcggccag 
      ttcgtgaat ttgtccgat tggtgtcac agcacgttc 
      ccgtttact tttaccagt ttacatacg agggtggat 
      tgtggggtt ttgcgaata ggacgcaac atggcgtaa 
      cacatagtg aagaattgg ggtatttgt ggagatcaa 
      agtttagtc cttccgaat cggatctgc ttatgagga 
      aaagaatga cttcaatgt ggacagatt ttgcaaaaa 
      ctggaaatc caacgccaa aaaaatatt tcacgtcct 
      ctgaagtca tctggaaaa ccaccgatt ctaatttca 
      cttatctcg atatcggtg atgaactat tactaagtc 
      aacatccta ataaagcta gcatgactt tgtgggacc 
      agatctacc aactttacg gaaatccac cttatgaca 
      cgtattaat ttttgtata attaaaata gtggagaat 
      cgtagaata ataaaattc gatttttaa t