Status
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Gene information
General information
TC032040
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000799,TC000800
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 325766..331360
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032040
    iBeetle screen iB_03078
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032040 RA
      MRLFGCVAL VLLLSIFTA YGFPSKQTE LIELSVIHI 
      NDFHARYEE TNLDSGLCK TSNCIGGFS RLFASVMKI 
      IEEKPHSIL LNAGDNFEG TLWYDVHKW NVTQYFFNK 
      LPTDAFTLG NHEFEDKIA GLVPFLEQL KAPVVVSNI 
      DATREPSIQ NLFNKSVVI ERHGLKIGI IGVILSTIN 
      EIADIGNLI LLKESESVN TEAERLVRE EGVFTNIVL 
      SHCGYDVEK AIAANATSK ISLVVGGHT HSFLYTGEP 
      VPGTDIPVG PYPTIVQNK EGKKVLVVQ ASSYTKYLG 
      NISLFYNNF GEVVNWTGA PVFMDSKLP QDKEVNEEL 
      QPWKKQVDA NEGNRIIGQ TMVRLEQSS CPMAECLMG 
      NFITDAMVF GYLDHPEPG TWTNASIAM MHAGGLRSG 
      IDIGSITYY EATSIAPFN NTIDIGEIQ GKHLKEIFE 
      SSTSDDGIT LLQVAGLKI VYNLTMPEG SRVTFLQVR 
      CRKCTVPVY EDLKDEEMY RVVLPSFLA EGGFGFTLF 
      KKYFKNRQI GGRDRDLFV RYVEICLKC CVVNYSIKF 
      AMQTFLPIV LFLKIVLCA QFNLTIIHF NDFHARYEP 
      VDNLSTTCE GDNCIGGFS RLYSKITQL KNETPNSLL 
      LNAGDNFQG TLWYIVHKW NVTQHFLNK LPFDAITLG 
      NHEFDDGIA GLVPYLNHI KAPVIVSNI DDSKEPRFQ 
      NLTRKSVIV EKGGKKIGI IGVLTTSTK LTSRTENLT 
      FQNELESVN SEAKKLQEL GVFTIIVLS HCGYEADLE 
      IAQKSNFKI SVIVGGHTD TFLYSGSPV PGPDKPSGP 
      YPTPVLNIN GGTVLVVQA SAYTKYLGD LKVIYDELG 
      NLTGWGGNP VFLGPETPS DGEIDAELE VWKKDVDLE 
      GGKIVGNSK LAMRNKPCR SEECPLGNF ATDAMVENN 
      NTCNLAMIH AGGMRAGIE TGNITFNQL ISTFPFQDT 
      INLCEIKGE HLVKILESC VDDNHLKWP HLFQVSGFK 
      IVFNVSNPE GGRVQSVKT SCRECADPI YENLVLEKY 
      YRVFLPTFL TDTDFNPLF KKYIRNKII GDRDSDVYV 
      RYLGKHSPL WARVEDRIK IVS
    CDS sequence
    • >TC032040 RA
      atgagactt ttcggttgt gttgctttg gtactatta 
      ctttcaata tttactgcc tatggcttt ccgtctaaa 
      caaaccgaa ttgatcgaa ttgtccgtc attcacata 
      aatgatttt catgcaaga tatgaggaa acaaacctc 
      gattccggg ttatgtaaa acctcaaac tgtattggt 
      ggattctcg cgacttttt gcaagtgtt atgaaaatt 
      atcgaggaa aaacctcac tcgatttta ctcaatgcg 
      ggcgataat ttcgagggt actttatgg tacgatgtt 
      cacaaatgg aacgttact cagtacttt ttcaataag 
      ctaccaaca gacgcattt actttgggt aaccacgag 
      tttgaagat aaaatcgca ggtttagta ccgtttttg 
      gagcaactc aaagcccca gttgttgtg tcaaatatt 
      gatgcaact cgggaacca tcaattcag aatttgttc 
      aacaaaagt gtagtgata gaaagacac gggttgaaa 
      attggcata attggagtc attttatcc acaatcaat 
      gaaattgca gacattggt aatttaatt ttactaaaa 
      gagtctgaa tcggtaaat acggaggcc gaaagatta 
      gttagggaa gaaggagtg tttacgaat attgtttta 
      tcacactgt ggctacgac gttgaaaag gcaatcgca 
      gcaaacgca acttcaaaa atttcgctg gttgtggga 
      ggccacact catagtttt ctctacact ggagaaccc 
      gtacctgga accgacata ccagtgggt ccatacccc 
      acaatcgtc caaaacaaa gaagggaaa aaagttcta 
      gtagtccaa gcctcgtct tataccaaa tatctcgga 
      aatattagc cttttttat aataacttt ggcgaagtc 
      gtgaattgg accggtgct cctgtcttc atggacagt 
      aaactacct caagataaa gaagtgaat gaggaacta 
      caaccttgg aagaaacaa gtcgatgca aatgaaggt 
      aatagaatt attggtcaa acaatggtt cgcttggaa 
      caaagttcg tgtcccatg gccgaatgt cttatgggg 
      aatttcata acagatgca atggttttt gggtattta 
      gaccatcca gaacctggc acttggaca aatgcatca 
      attgcgatg atgcatgct ggaggttta agatcaggg 
      attgatatt gggagtata acttactac gaagctaca 
      agcatagca ccctttaac aacaccatc gatattggt 
      gaaatccaa ggaaaacac ctgaaggaa atcttcgaa 
      tcttccacc agtgacgat ggtatcaca cttctgcaa 
      gtggctggc cttaaaatc gtatacaac ctaacaatg 
      cccgaaggt tcgcgagtg acgttcctc caagtccgt 
      tgtcgaaaa tgcacagtt ccagtttat gaggattta 
      aaagacgaa gaaatgtat agagttgtc ttaccatcg 
      tttttagcc gaaggtggt ttcgggttc actttgttc 
      aaaaagtac ttcaaaaat cggcaaata ggaggacga 
      gatagagat ttgtttgtg agatacgtc gagatttgt 
      ttgaagtgt tgtgttgtt aattactca ataaaattc 
      gcaatgcaa acgtttttg ccaattgtt ttatttttg 
      aaaattgtt ctttgtgca caatttaat ttaactata 
      attcatttc aatgatttt cacgccagg tacgaaccc 
      gttgataat ttatcaaca acttgtgaa ggcgataat 
      tgtattggc ggtttctca agactgtat tcaaaaatc 
      acacaatta aaaaacgaa acaccaaac tcactttta 
      ttaaatgct ggagataat tttcagggg actttatgg 
      tacattgtg cacaaatgg aatgtcact caacacttt 
      ttaaataaa ctaccgttt gatgcaatt actcttgga 
      aatcacgaa tttgacgac ggtattgcc ggtttagtg 
      ccctattta aaccacatt aaagcacca gttattgtg 
      agcaatatt gacgatagt aaggaaccc cgatttcaa 
      aatttgacc aggaagagc gtaatcgtt gaaaaaggc 
      ggcaaaaag atcggaatt attggagtc ctcaccact 
      tcgactaaa ttgacttct cgcaccgaa aatttgacc 
      tttcaaaat gaattagaa tcggtgaat tctgaagct 
      aaaaaattg caagaactt ggtgttttc actattatt 
      gttctctct cattgtggt tacgaggcc gatttggaa 
      attgcacaa aagtccaat ttcaaaata agtgtgata 
      gttgggggc cacactgac acttttctt tatagcgga 
      agtccagtc cccggtcca gacaaacct tctggacct 
      taccctact cctgtttta aacataaat ggtggtaca 
      gttctagtt gtgcaagca tctgcttac actaaatat 
      cttggtgat cttaaagta atttatgat gagcttggt 
      aatttaaca ggatggggc ggaaatcct gtgtttttg 
      gggccagaa acaccgtca gatggggaa attgatgca 
      gaactggaa gtttggaag aaggatgtg gacttagaa 
      ggtgggaaa attgtggga aactctaaa cttgcgatg 
      agaaataag ccgtgtagg tctgaagaa tgtcccctt 
      ggaaatttt gcaacagat gcaatggtt gaaaacaac 
      aacacatgc aaccttgct atgatacat gccggagga 
      atgagggcc ggaattgaa acaggaaat attacgttc 
      aatcaattg atatcaacg tttccgttt caagacact 
      atcaaccta tgtgaaata aaaggggaa catttggtc 
      aaaattttg gaaagttgt gtggatgat aatcacttg 
      aaatggccc catttattc caagtatct ggttttaaa 
      atcgtattc aatgtcagt aatcctgaa ggtggcaga 
      gttcaatcg gtaaaaacc agttgcaga gaatgtgcc 
      gaccctatt tacgaaaat cttgttttg gagaaatat 
      tatcgagtt ttcttgcca actttccta actgatacc 
      gatttcaat ccacttttt aagaaatac ataagaaat 
      aaaataatt ggtgatcgg gactcagac gtttatgtg 
      agatacttg ggaaaacat tctccatta tgggcaaga 
      gttgaggat aggattaaa attgtgtcg tag
    mRNA sequence
    • >TC032040 RA
      ttttattat tttattgtg ctgcttacg tgcgccaca 
      tcaagaaga aattagttc ctaaaacaa acatgagac 
      ttttcggtt gtgttgctt tggtactat tactttcaa 
      tatttactg cctatggct ttccgtcta aacaaaccg 
      aattgatcg aattgtccg tcattcaca taaatgatt 
      ttcatgcaa gatatgagg aaacaaacc tcgattccg 
      ggttatgta aaacctcaa actgtattg gtggattct 
      cgcgacttt ttgcaagtg ttatgaaaa ttatcgagg 
      aaaaacctc actcgattt tactcaatg cgggcgata 
      atttcgagg gtactttat ggtacgatg ttcacaaat 
      ggaacgtta ctcagtact ttttcaata agctaccaa 
      cagacgcat ttactttgg gtaaccacg agtttgaag 
      ataaaatcg caggtttag taccgtttt tggagcaac 
      tcaaagccc cagttgttg tgtcaaata ttgatgcaa 
      ctcgggaac catcaattc agaatttgt tcaacaaaa 
      gtgtagtga tagaaagac acgggttga aaattggca 
      taattggag tcattttat ccacaatca atgaaattg 
      cagacattg gtaatttaa ttttactaa aagagtctg 
      aatcggtaa atacggagg ccgaaagat tagttaggg 
      aagaaggag tgtttacga atattgttt tatcacact 
      gtggctacg acgttgaaa aggcaatcg cagcaaacg 
      caacttcaa aaatttcgc tggttgtgg gaggccaca 
      ctcatagtt ttctctaca ctggagaac ccgtacctg 
      gaaccgaca taccagtgg gtccatacc ccacaatcg 
      tccaaaaca aagaaggga aaaaagttc tagtagtcc 
      aagcctcgt cttatacca aatatctcg gaaatatta 
      gcctttttt ataataact ttggcgaag tcgtgaatt 
      ggaccggtg ctcctgtct tcatggaca gtaaactac 
      ctcaagata aagaagtga atgaggaac tacaacctt 
      ggaagaaac aagtcgatg caaatgaag gtaatagaa 
      ttattggtc aaacaatgg ttcgcttgg aacaaagtt 
      cgtgtccca tggccgaat gtcttatgg ggaatttca 
      taacagatg caatggttt ttgggtatt tagaccatc 
      cagaacctg gcacttgga caaatgcat caattgcga 
      tgatgcatg ctggaggtt taagatcag ggattgata 
      ttgggagta taacttact acgaagcta caagcatag 
      cacccttta acaacacca tcgatattg gtgaaatcc 
      aaggaaaac acctgaagg aaatcttcg aatcttcca 
      ccagtgacg atggtatca cacttctgc aagtggctg 
      gccttaaaa tcgtataca acctaacaa tgcccgaag 
      gttcgcgag tgacgttcc tccaagtcc gttgtcgaa 
      aatgcacag ttccagttt atgaggatt taaaagacg 
      aagaaatgt atagagttg tcttaccat cgtttttag 
      ccgaaggtg gtttcgggt tcactttgt tcaaaaagt 
      acttcaaaa atcggcaaa taggaggac gagatagag 
      atttgtttg tgagatacg tcgagattt gtttgaagt 
      gttgtgttg ttaattact caataaaat tcgcaatgc 
      aaacgtttt tgccaattg ttttatttt tgaaaattg 
      ttctttgtg cacaattta atttaacta taattcatt 
      tcaatgatt ttcacgcca ggtacgaac ccgttgata 
      atttatcaa caacttgtg aaggcgata attgtattg 
      gcggtttct caagactgt attcaaaaa tcacacaat 
      taaaaaacg aaacaccaa actcacttt tattaaatg 
      ctggagata attttcagg ggactttat ggtacattg 
      tgcacaaat ggaatgtca ctcaacact ttttaaata 
      aactaccgt ttgatgcaa ttactcttg gaaatcacg 
      aatttgacg acggtattg ccggtttag tgccctatt 
      taaaccaca ttaaagcac cagttattg tgagcaata 
      ttgacgata gtaaggaac cccgatttc aaaatttga 
      ccaggaaga gcgtaatcg ttgaaaaag gcggcaaaa 
      agatcggaa ttattggag tcctcacca cttcgacta 
      aattgactt ctcgcaccg aaaatttga cctttcaaa 
      atgaattag aatcggtga attctgaag ctaaaaaat 
      tgcaagaac ttggtgttt tcactatta ttgttctct 
      ctcattgtg gttacgagg ccgatttgg aaattgcac 
      aaaagtcca atttcaaaa taagtgtga tagttgggg 
      gccacactg acacttttc tttatagcg gaagtccag 
      tccccggtc cagacaaac cttctggac cttacccta 
      ctcctgttt taaacataa atggtggta cagttctag 
      ttgtgcaag catctgctt acactaaat atcttggtg 
      atcttaaag taatttatg atgagcttg gtaatttaa 
      caggatggg gcggaaatc ctgtgtttt tggggccag 
      aaacaccgt cagatgggg aaattgatg cagaactgg 
      aagtttgga agaaggatg tggacttag aaggtggga 
      aaattgtgg gaaactcta aacttgcga tgagaaata 
      agccgtgta ggtctgaag aatgtcccc ttggaaatt 
      ttgcaacag atgcaatgg ttgaaaaca acaacacat 
      gcaaccttg ctatgatac atgccggag gaatgaggg 
      ccggaattg aaacaggaa atattacgt tcaatcaat 
      tgatatcaa cgtttccgt ttcaagaca ctatcaacc 
      tatgtgaaa taaaagggg aacatttgg tcaaaattt 
      tggaaagtt gtgtggatg ataatcact tgaaatggc 
      cccatttat tccaagtat ctggtttta aaatcgtat 
      tcaatgtca gtaatcctg aaggtggca gagttcaat 
      cggtaaaaa ccagttgca gagaatgtg ccgacccta 
      tttacgaaa atcttgttt tggagaaat attatcgag 
      ttttcttgc caactttcc taactgata ccgatttca 
      atccacttt ttaagaaat acataagaa ataaaataa 
      ttggtgatc gggactcag acgtttatg tgagatact 
      tgggaaaac attctccat tatgggcaa gagttgagg 
      ataggatta aaattgtgt cgtagaagt tgttaattt 
      tttattt