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Gene information
General information
TC000792 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 205866..211790
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000792
    iBeetle screen iB_03077
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000792 RA
      MEIERTGNK LSWSELKAV VSDLRRQLS SLSTMIPTA 
      VSFRTLQDG RTRIYFLST PQNGWETTL LYADVPNVN 
      QPTSLRLQW QCVIESNFP SLSSTNKFS REEQLLWER 
      KRLATWGIN TYEIHSESG KIIFPASSS LFQCIDNGY 
      SNGPLFPTK LRMTSAGAK LNPQICPSN PDLVAYICN 
      HDIWVTHTV SGSNVRLTY AHKGGRYLA DDPLCAGVP 
      SYVMQEEFC RYQGYWWQP TCTGGVYRI LYEEVDDGD 
      VKIFCFPSS QSDTGEVEE FRFPRAGCA NSKSYLKMV 
      EFKVNEHRQ IVDICMLEL QFSLSMLFP WMEYIVRVG 
      WTPDSEYIW VQLLDRRQQ KLELVLLSL SNFTEVLPM 
      YEDSPNVQF GRPVVQVIY SQESSIWVN VHDLLCFLP 
      STDHNNEVQ FIWASEDTG FRHLYLITS RIIPYSNGV 
      TESTEPMHY IFLQPKIIS KVALTSGEW EVLAQNIWV 
      DYERQLVYF MGLKETPLE KHLYVVSLR RPGEVRLLT 
      RPGFSYSVD FNEDCTMIV TVYSNIQKP PACQVFRIS 
      HTDWTVEGV TLTPIGYLL ESSALANDN YCPELYTHE 
      ISSGNVLYA MVFKPHHFE PGKKYPTVL NIYGGPEVQ 
      LVSNSFKGM RQLRMHMLA ARGYCVISI DSRGSQHRG 
      LLFESHIKR RMGTVELDD QVEVLKWLA ENLDFIDLN 
      RVAIHGWSY GGYLSLMGL IHYSDIFKI AIAGAPVTN 
      WNLYDTGYT ERYMDLPEH NPQGYMEGS VLNYINKFP 
      EEENRLLII HGLIDENVH FYHTSQLIN SMVKAGKPY 
      QLQIYPNER HSLRHIDAS KHYETTLLS FLQNHL
    CDS sequence
    • >TC000792 RA
      atggaaata gagcggact gggaataaa ttgagttgg 
      tcggagctt aaggccgtt gtgagcgac ttgagacgg 
      caattatca agcctctcc acaatgatc ccaacggct 
      gtttccttc cggacttta caagatggc aggactcga 
      atttatttt ttaagcaca ccacagaac ggctgggag 
      accactctc ttatatgca gacgtgccc aacgttaac 
      caacccact agtttgcgc ttgcagtgg cagtgcgtg 
      attgaatca aatttcccc agtctgtca tcaacgaat 
      aagttttcg cgggaggaa cagctgctt tgggagagg 
      aagcggttg gctacttgg ggcatcaac acttacgag 
      attcacagt gaatccggg aaaatcatt tttcccgcg 
      tccagtagc cttttccag tgcattgat aacggatat 
      tcaaacggt cccctattt cccacaaaa ttacgaatg 
      acttcggct ggagcgaag ctaaatcca caaatctgc 
      ccatcaaat cctgatctc gtagcgtat atttgtaac 
      catgatatt tgggtcacc cataccgtt tctgggtca 
      aatgttcgc ttaacgtat gcccataaa gggggcagg 
      taccttgcg gatgaccca ctgtgtgct ggggtaccg 
      tcgtatgtc atgcaagag gagttttgc aggtaccaa 
      ggctactgg tggcagcct acatgcaca ggcggtgtt 
      tatagaatt ttatacgag gaagtcgac gacggtgac 
      gtgaaaatc ttttgtttt ccctcatct cagtccgac 
      accggcgaa gtggaagaa ttccgcttt cctcgagcg 
      ggctgtgca aactcaaaa tcgtattta aaaatggtc 
      gagttcaaa gtaaacgaa caccgccaa atcgtagat 
      atttgtatg ttagaatta cagttttct ctatcaatg 
      ttattccct tggatggaa tacattgtt agagttggt 
      tggacccca gactccgaa tatatttgg gtacagttg 
      ctcgaccga aggcaacaa aaactcgaa ctggttctc 
      ctttcttta agtaacttt accgaagtg cttccaatg 
      tacgaggac tcgcccaac gtgcaattc ggtcgtcct 
      gtagtccaa gtaatctat tcacaagaa tcgagtata 
      tgggtcaat gtgcatgac ttgctgtgt tttttgccg 
      tccactgat cacaataac gaagtgcaa tttatatgg 
      gcttcagag gacacaggt ttccgacac ttgtattta 
      attacgtcc aggattata ccatacagt aacggcgtc 
      accgaaagt acagaacca atgcattac atattttta 
      caaccgaaa atcatatca aaagtcgct ttaacgtca 
      ggcgaatgg gaggttttg gcccaaaat atttgggtc 
      gactacgag agacaacta gtttatttc atggggctc 
      aaggagacg cctttggag aaacactta tacgtggtg 
      tctttaagg cgaccgggc gaagtccgc ttgttgacc 
      agaccaggg ttttcctat tcagttgac ttcaatgag 
      gactgtacg atgatagtc accgtttat agtaacatt 
      caaaaaccc ccagcttgt caagtcttc cggatatct 
      cacacggac tggaccgtt gaaggagtt acgttaact 
      ccgattggt tacttgctg gaatcgagc gctttagcc 
      aatgataat tattgccca gaactgtac acccatgaa 
      atatcgtca gggaatgtt ttatatgcg atggtcttc 
      aagccgcat cattttgaa ccggggaaa aagtaccca 
      acggtgttg aacatttat gggggcccg gaggtccag 
      cttgtctca aactcgttc aagggaatg aggcagttg 
      aggatgcac atgttggca gctaggggc tactgtgtc 
      atatcaata gattccagg ggttcccaa caccggggt 
      ttgttgttt gagagccac attaaacgg aggatgggt 
      accgtggag cttgatgat caagttgaa gtattaaaa 
      tggttggcc gaaaattta gattttata gatttgaat 
      cgggttgcg atacatggg tggtcttac gggggctat 
      ttaagcctg atgggtctg atacattat tcagacatt 
      ttcaaaatt gcgatagct ggggctcct gttactaac 
      tggaatctg tatgatacg ggttatact gaacgatat 
      atggacttg cctgaacac aatcctcaa ggatatatg 
      gagggatca gtccttaat tacattaat aagtttcca 
      gaagaggaa aaccggctg ctaataatc cacggcctc 
      attgacgag aacgttcat ttttatcac acgagtcag 
      ttgatcaac agtatggta aaggcgggt aaaccttac 
      cagttgcaa atctacccc aatgaaagg cactcgttg 
      cgacacatc gacgcgagt aaacattat gaaacgacc 
      ttgctatcc ttcctccaa aatcactta taa
    mRNA sequence
    • >TC000792 RA
      ggtgacaag taaataagg ttaactttt tatttgggt 
      caaaaaaga aaagtttac atgaatatc tcagtattt 
      cgttaaaaa ataatgatc agggtgaat tgaactcgc 
      attgttcta tcattagtt tatgaatac cagatgtga 
      tagtttata aactatgga aatagagcg gactgggaa 
      taaattgag ttggtcgga gcttaaggc cgttgtgag 
      cgacttgag acggcaatt atcaagcct ctccacaat 
      gatcccaac ggctgtttc cttccggac tttacaaga 
      tggcaggac tcgaattta ttttttaag cacaccaca 
      gaacggctg ggagaccac tctcttata tgcagacgt 
      gcccaacgt taaccaacc cactagttt gcgcttgca 
      gtggcagtg cgtgattga atcaaattt ccccagtct 
      gtcatcaac gaataagtt ttcgcggga ggaacagct 
      gctttggga gaggaagcg gttggctac ttggggcat 
      caacactta cgagattca cagtgaatc cgggaaaat 
      catttttcc cgcgtccag tagcctttt ccagtgcat 
      tgataacgg atattcaaa cggtcccct atttcccac 
      aaaattacg aatgacttc ggctggagc gaagctaaa 
      tccacaaat ctgcccatc aaatcctga tctcgtagc 
      gtatatttg taaccatga tatttgggt cacccatac 
      cgtttctgg gtcaaatgt tcgcttaac gtatgccca 
      taaaggggg caggtacct tgcggatga cccactgtg 
      tgctggggt accgtcgta tgtcatgca agaggagtt 
      ttgcaggta ccaaggcta ctggtggca gcctacatg 
      cacaggcgg tgtttatag aattttata cgaggaagt 
      cgacgacgg tgacgtgaa aatcttttg ttttccctc 
      atctcagtc cgacaccgg cgaagtgga agaattccg 
      ctttcctcg agcgggctg tgcaaactc aaaatcgta 
      tttaaaaat ggtcgagtt caaagtaaa cgaacaccg 
      ccaaatcgt agatatttg tatgttaga attacagtt 
      ttctctatc aatgttatt cccttggat ggaatacat 
      tgttagagt tggttggac cccagactc cgaatatat 
      ttgggtaca gttgctcga ccgaaggca acaaaaact 
      cgaactggt tctcctttc tttaagtaa ctttaccga 
      agtgcttcc aatgtacga ggactcgcc caacgtgca 
      attcggtcg tcctgtagt ccaagtaat ctattcaca 
      agaatcgag tatatgggt caatgtgca tgacttgct 
      gtgtttttt gccgtccac tgatcacaa taacgaagt 
      gcaatttat atgggcttc agaggacac aggtttccg 
      acacttgta tttaattac gtccaggat tataccata 
      cagtaacgg cgtcaccga aagtacaga accaatgca 
      ttacatatt tttacaacc gaaaatcat atcaaaagt 
      cgctttaac gtcaggcga atgggaggt tttggccca 
      aaatatttg ggtcgacta cgagagaca actagttta 
      tttcatggg gctcaagga gacgccttt ggagaaaca 
      cttatacgt ggtgtcttt aaggcgacc gggcgaagt 
      ccgcttgtt gaccagacc agggttttc ctattcagt 
      tgacttcaa tgaggactg tacgatgat agtcaccgt 
      ttatagtaa cattcaaaa acccccagc ttgtcaagt 
      cttccggat atctcacac ggactggac cgttgaagg 
      agttacgtt aactccgat tggttactt gctggaatc 
      gagcgcttt agccaatga taattattg cccagaact 
      gtacaccca tgaaatatc gtcagggaa tgttttata 
      tgcgatggt cttcaagcc gcatcattt tgaaccggg 
      gaaaaagta cccaacggt gttgaacat ttatggggg 
      cccggaggt ccagcttgt ctcaaactc gttcaaggg 
      aatgaggca gttgaggat gcacatgtt ggcagctag 
      gggctactg tgtcatatc aatagattc caggggttc 
      ccaacaccg gggtttgtt gtttgagag ccacattaa 
      acggaggat gggtaccgt ggagcttga tgatcaagt 
      tgaagtatt aaaatggtt ggccgaaaa tttagattt 
      tatagattt gaatcgggt tgcgataca tgggtggtc 
      ttacggggg ctatttaag cctgatggg tctgataca 
      ttattcaga cattttcaa aattgcgat agctggggc 
      tcctgttac taactggaa tctgtatga tacgggtta 
      tactgaacg atatatgga cttgcctga acacaatcc 
      tcaaggata tatggaggg atcagtcct taattacat 
      taataagtt tccagaaga ggaaaaccg gctgctaat 
      aatccacgg cctcattga cgagaacgt tcattttta 
      tcacacgag tcagttgat caacagtat ggtaaaggc 
      gggtaaacc ttaccagtt gcaaatcta ccccaatga 
      aaggcactc gttgcgaca catcgacgc gagtaaaca 
      ttatgaaac gaccttgct atccttcct ccaaaatca 
      cttataatt cgtttgcat tgttcatag tcaagcagt 
      attaaaaga aaattaaat aaaagtcga ttagagaat 
      tcaacaagt gtttagtga gacaatttg tttcttact 
      gtatgttaa atgtggatt gaagtcagc tttgtaaca 
      agttttaaa cgcaattgt aaataattc tacttatca 
      caactacta aggctttta attcgattg tataaatta 
      aattattta tatgaattt ctaaaaatt aaagcaact 
      gtttttacc gttacaaat cttttattt ttacatgaa 
      attaattac acagttttt gttttctta tttagaaag 
      cggtagagg taattgatt cgtttttgt aactggttc 
      cactttgtt ggccgtgca aggggatat acggttttt 
      gccaacatg tttcttttc ctgagcatc acctggaac 
      tcatttccg ccaactcct cttaaccaa ggtggaata 
      ttgtacaat attctggta ggatgtaaa cgttcgttc 
      cccaaaata tcaatgtag tcgttttgg ccaaataat 
      gcccttttc tcggtccac gcatctttg ggtgtgtat 
      aggggttta cataacctg tcagccccc ttgagtcgc 
      ggtaaagtc cccctttga agaattttt tcttcataa 
      ccataacgt cttaacaac ggacctctt gccacttta 
      tcggcatga taacggaaa cgcacctgg agggcttca 
      ggtaagcac acttttccg atattaaaa gctacagtt 
      aatttattt aataacatt ttagtcttc ttatataaa 
      gttaggtta ggaacatag aataagaga g