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Gene information
General information
TC000750 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6235144..6238314
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000750
    iBeetle screen iB_03073

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000750 RA
      MSLEDPLYH ITPVQQAAA SCTGAVLTS LLVTPLDVV 
KIRLQAQHR LSQNVSNKC FLYCNGLMD HFCGCTPNN 
GQKHWFQRP GHFNGTIDA FIKITKNEG IYSLWSGLG 
PTLVLALPT TILYFVTYE QLRLRLKNL YNRNNVEGQ 
ERKQPYWIP LISGATARI FAVSVVSPL ELIRTKMQS 
RKISYAEIN ESLKLLIKQ DGIKGLWKG VFPTLGRDV 
PFSAIYWMN YETIKGFFG SDTPTFGVS FFAGAVSGG 
IAAFATVPF DVVKTHQQI EIGEKTLYT DKPQRTKRT 
AQIIREIYR HSGIKGLYA GLVPRLVKV APACAIMIS 
SFEYGKVFF YRYNEFMYN HGASLQEVT DFYAEEYFP 
PEHN
    • >TC000750 RB
      MSLEDPLYH ITPVQQAAA SCTGAVLTS LLVTPLDVV 
KIRLQAQHR LSQNVSNKC FLYCNGLMD HFCGCTPNN 
GQKHWFQRP GHFNGTIDA FIKITKNEG IYSLWSGLG 
PTLVLALPT TILYFVTYE QLRLRLKNL YNRNNVEGQ 
ERKQPYWIP LISGATARI FAVSVVSPL ELIRTKMQS 
RKISYAEIN ESLKLLIKQ DGIKGLWKG VFPTLGRDV 
PFSAIYWMN YETIKGFFG SDTPTFGVS FFAGAVSGG 
IAAFATVPF DVVKTHQQI EIGEKTLYT DKPQRTKRT 
AQIIREIYR HSGIKGLYA GLVPRLVKV APACAIMIS 
SFEYGKVFF YRLANPDSD IFNSSKENL QSNK
    CDS sequence
    • >TC000750 RA
      atgtcatta gaggatccg ctatatcac ataactcca 
gtgcaacaa gcggccgct tcatgtacg ggggctgtt 
ctaacctca cttttagtc actccatta gacgtagtc 
aaaattaga ttacaagct cagcatcgc ttatctcaa 
aatgtgtcc aataaatgt tttttatac tgtaatggg 
cttatggac catttttgt ggttgtact ccaaacaat 
gggcagaag cattggttt caaagacct gggcacttt 
aacggaact atagacgct tttattaaa attacgaaa 
aatgaagga atttattca ttgtggagc ggtttagga 
cctactcta gttctagca cttccgacc acaattttg 
tattttgtg acttatgaa cagttaaga ttaagattg 
aagaatttg tacaataga aacaacgtg gagggacaa 
gagaggaaa cagccttat tggattcca ttaatctca 
ggtgcaact gccagaata tttgctgtt tctgttgta 
agtccttta gaattgatt agaactaaa atgcaatcg 
cggaaaata tcgtacgca gaaataaac gaatcttta 
aaattgttg attaagcaa gacgggatc aagggatta 
tggaagggt gtttttcca actttggga agggatgtt 
cccttctct gccatttac tggatgaat tacgagacc 
atcaaaggt ttttttggg tcagacact ccaactttt 
ggcgttagt tttttcgct ggggctgtg tcaggaggg 
attgctgct tttgccaca gttccgttt gatgtcgtt 
aaaacccac caacagatc gaaattggt gaaaaaact 
ctttatacc gataaacca caaagaaca aaacgtaca 
gcacaaata ataagggaa atttataga catagcggt 
attaaagga ctgtatgcc ggtttagtt cctcgactt 
gttaaagtt gccccagcg tgtgctatt atgatatcg 
tcatttgaa tatggaaag gtatttttc tacagatat 
aacgaattt atgtataat catggtgcg agtctacaa 
gaagttacc gatttttac gccgaagaa tatttccca 
cctgaacat aattaa
    • >TC000750 RB
      atgtcatta gaggatccg ctatatcac ataactcca 
gtgcaacaa gcggccgct tcatgtacg ggggctgtt 
ctaacctca cttttagtc actccatta gacgtagtc 
aaaattaga ttacaagct cagcatcgc ttatctcaa 
aatgtgtcc aataaatgt tttttatac tgtaatggg 
cttatggac catttttgt ggttgtact ccaaacaat 
gggcagaag cattggttt caaagacct gggcacttt 
aacggaact atagacgct tttattaaa attacgaaa 
aatgaagga atttattca ttgtggagc ggtttagga 
cctactcta gttctagca cttccgacc acaattttg 
tattttgtg acttatgaa cagttaaga ttaagattg 
aagaatttg tacaataga aacaacgtg gagggacaa 
gagaggaaa cagccttat tggattcca ttaatctca 
ggtgcaact gccagaata tttgctgtt tctgttgta 
agtccttta gaattgatt agaactaaa atgcaatcg 
cggaaaata tcgtacgca gaaataaac gaatcttta 
aaattgttg attaagcaa gacgggatc aagggatta 
tggaagggt gtttttcca actttggga agggatgtt 
cccttctct gccatttac tggatgaat tacgagacc 
atcaaaggt ttttttggg tcagacact ccaactttt 
ggcgttagt tttttcgct ggggctgtg tcaggaggg 
attgctgct tttgccaca gttccgttt gatgtcgtt 
aaaacccac caacagatc gaaattggt gaaaaaact 
ctttatacc gataaacca caaagaaca aaacgtaca 
gcacaaata ataagggaa atttataga catagcggt 
attaaagga ctgtatgcc ggtttagtt cctcgactt 
gttaaagtt gccccagcg tgtgctatt atgatatcg 
tcatttgaa tatggaaag gtatttttc tacaggtta 
gccaatcca gattcagat atctttaat tccagcaaa 
gaaaactta caatcaaat aaataa
    mRNA sequence
    • >TC000750 RA
      tgtcaaccc tgaactgtc agtagtgtg attcccttt 
tgttactta tcacaaaat ttgggtttt tcgttgaaa 
attttaaat gataacaat aagcgacag tctgtaatt 
gattagaga ccaaaaatg tcattagag gatccgcta 
tatcacata actccagtg caacaagcg gccgcttca 
tgtacgggg gctgttcta acctcactt ttagtcact 
ccattagac gtagtcaaa attagatta caagctcag 
catcgctta tctcaaaat gtgtccaat aaatgtttt 
ttatactgt aatgggctt atggaccat ttttgtggt 
tgtactcca aacaatggg cagaagcat tggtttcaa 
agacctggg cactttaac ggaactata gacgctttt 
attaaaatt acgaaaaat gaaggaatt tattcattg 
tggagcggt ttaggacct actctagtt ctagcactt 
ccgaccaca attttgtat tttgtgact tatgaacag 
ttaagatta agattgaag aatttgtac aatagaaac 
aacgtggag ggacaagag aggaaacag ccttattgg 
attccatta atctcaggt gcaactgcc agaatattt 
gctgtttct gttgtaagt cctttagaa ttgattaga 
actaaaatg caatcgcgg aaaatatcg tacgcagaa 
ataaacgaa tctttaaaa ttgttgatt aagcaagac 
gggatcaag ggattatgg aagggtgtt tttccaact 
ttgggaagg gatgttccc ttctctgcc atttactgg 
atgaattac gagaccatc aaaggtttt tttgggtca 
gacactcca acttttggc gttagtttt ttcgctggg 
gctgtgtca ggagggatt gctgctttt gccacagtt 
ccgtttgat gtcgttaaa acccaccaa cagatcgaa 
attggtgaa aaaactctt tataccgat aaaccacaa 
agaacaaaa cgtacagca caaataata agggaaatt 
tatagacat agcggtatt aaaggactg tatgccggt 
ttagttcct cgacttgtt aaagttgcc ccagcgtgt 
gctattatg atatcgtca tttgaatat ggaaaggta 
tttttctac agatataac gaatttatg tataatcat 
ggtgcgagt ctacaagaa gttaccgat ttttacgcc 
gaagaatat ttcccacct gaacataat taatttgta 
ttatgtact tttagaaat aaatattaa gttgtagtc 
aaaaaattg acataaacc gccacaaat aaattgtta 
ttgtttta
    • >TC000750 RB
      tgtcaaccc tgaactgtc agtagtgtg attcccttt 
tgttactta tcacaaaat ttgggtttt tcgttgaaa 
attttaaat gataacaat aagcgacag tctgtaatt 
gattagaga ccaaaaatg tcattagag gatccgcta 
tatcacata actccagtg caacaagcg gccgcttca 
tgtacgggg gctgttcta acctcactt ttagtcact 
ccattagac gtagtcaaa attagatta caagctcag 
catcgctta tctcaaaat gtgtccaat aaatgtttt 
ttatactgt aatgggctt atggaccat ttttgtggt 
tgtactcca aacaatggg cagaagcat tggtttcaa 
agacctggg cactttaac ggaactata gacgctttt 
attaaaatt acgaaaaat gaaggaatt tattcattg 
tggagcggt ttaggacct actctagtt ctagcactt 
ccgaccaca attttgtat tttgtgact tatgaacag 
ttaagatta agattgaag aatttgtac aatagaaac 
aacgtggag ggacaagag aggaaacag ccttattgg 
attccatta atctcaggt gcaactgcc agaatattt 
gctgtttct gttgtaagt cctttagaa ttgattaga 
actaaaatg caatcgcgg aaaatatcg tacgcagaa 
ataaacgaa tctttaaaa ttgttgatt aagcaagac 
gggatcaag ggattatgg aagggtgtt tttccaact 
ttgggaagg gatgttccc ttctctgcc atttactgg 
atgaattac gagaccatc aaaggtttt tttgggtca 
gacactcca acttttggc gttagtttt ttcgctggg 
gctgtgtca ggagggatt gctgctttt gccacagtt 
ccgtttgat gtcgttaaa acccaccaa cagatcgaa 
attggtgaa aaaactctt tataccgat aaaccacaa 
agaacaaaa cgtacagca caaataata agggaaatt 
tatagacat agcggtatt aaaggactg tatgccggt 
ttagttcct cgacttgtt aaagttgcc ccagcgtgt 
gctattatg atatcgtca tttgaatat ggaaaggta 
tttttctac aggttagcc aatccagat tcagatatc 
tttaattcc agcaaagaa aacttacaa tcaaataaa 
taattgtag atttaaggg cagctgaaa gcgattatt 
gcggtttta tttctaaaa gtttttaat tgtgttaat 
acggtgcga cctcgataa tacgaactc cgaaagaga 
gaaatattg cttattgaa ctaaatgtt tatttgtaa 
taaatctac gtttttttt taaagtctt caaaattgg 
tttgataaa gaaatactt ttggttggg aaaccaatg 
aaatcagac tttctcggc ttcgttttg tgtttgacg 
tttttcgtt tgttttaat aacgtccga acagttttt 
agtaaaatt atctttatt tattgtctt tatcatatt 
tgagaagaa aagtggaga aatgatgtc actgctctt 
gtgactaca atcgaagga aaaacttta cctataagg 
ctcgaaaag acaaaaata aatattatt ggttttcgg 
gctatttga gcatttcgc gagaaaagt gctcttatt 
taacaaaat tagcttgaa attaatttt taagaagac 
taaaataat ttttgagga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY