| General information | |||
| TC032066
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid7: 949916..954666 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032066 | |
| General information | ||||
| iB_03068 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032066 RA
MWWTRSTVT TVVLLTLIK PFHCQVVGI SCSVKNSDD LGVCKLLSE CKQVRDDIL KQQQLPQLC GFRETQAIV CCPQKKRKP RDISKIKCK EYSSYAKNE CGHKIVKRI IGGEFAGSK EFPHMALLG FEKKGSANN ITQWRCDGI IISDRYILT VAQCLNTRG FDLKYVKVG VTDLNETDH QQEIRVLQT VPHPDYKPL QSLRNDIGL IKLEKPIEL NSYARPACL YTERALAVD LAVVTGWGV TEAGSSSNK LKKVELPFV SSELCAVLF KYAKNGFDE DLQICAGTA NKDSCQGDT GGPLQIHHE GDDISCMYD IVGISSAGA RVCGTAPSI YTRVSHYVQ WIEDIVSYL LLSHKAKMV DATKRPLHL PPWHIPYLE KHRILELFH ELARELVIQ QPEDHVLFL KQVLINAAR NRDTARVVI ICSPKVNCL EIANAIAEI TKQNVITEH SLLTFSGRM EIQSPDLRA KYIGHLVRT DNAYENGWI LVDCIKNEN DAKALLKLG ILPTHVIHL IPSFQPPLT ELLYCHVPA EWPQYRRTV LGLRNAFKH VLREVFFQQ RGLQEIVAE CIEILNIKP AKYAIKPRV LILGPRGSG RKTQAKLLA QGLGLIHVD FEHLICEAW MSDSDLGKR LRNCKNEVC FHSELLAEV VHKRVLEDD CLEHGWVLT GFPFSKRDF EYLDCIYTP PNRVIFLEC DLAVCKERL LNRKINVDT GSVTNVKEH PEAEMSKQL KTHPKDNKE FIDAELSYY CENYGDLKK YCGASAVCL NGDQAERWI YETIVAIIS RAPPPHNPR RPLLDLSSS SVSLDACCC SPIPSQILD CYNTRL
- >TC032066 RA
atgtggtgg acccgttca acggtaaca acagtagtt ctactaact cttataaag ccttttcac tgtcaggtt gtcggtatt tcttgctct gtaaaaaat tccgatgac ttgggagtt tgcaaactg ttaagtgaa tgtaagcaa gtccgtgac gacatttta aaacaacaa caactacca caactatgc ggatttcgg gaaactcaa gcaatagtt tgttgtcca cagaaaaag cgaaaaccg cgtgacatt agcaaaata aaatgtaag gagtattct tcttatgcg aaaaacgaa tgcggacat aaaattgtt aaacgtatt ataggcgga gaatttgca gggagcaaa gaatttccg cacatggca ttattagga tttgaaaaa aaaggttca gctaataac ataacacaa tggagatgt gacggtata attattagc gaccgctac attttaact gtggcgcag tgtttaaat acgaggggt tttgatctt aaatatgta aaagtgggg gtcactgat cttaacgaa actgaccat caacaagaa ataagagtg ttacaaacc gttccacat cctgactat aaacctctt caaagtctc aggaatgat attggactt attaaatta gaaaaaccg atcgagtta aattcttat gcacgaccg gcttgtctc tacacagag agagcactt gcagttgat ttggctgta gtcactgga tggggtgtt acagaagca ggatctagc agcaataaa ttaaaaaaa gtagaacta ccttttgtg tccagtgaa ttgtgtgcc gtcttattt aaatatgct aaaaatggg ttcgatgaa gatttacaa atttgtgct ggaaccgca aataaggac agttgtcag ggcgacaca ggaggccca cttcagata catcatgaa ggtgatgat atttcctgc atgtatgat attgtggga atttcttct gctggggct agggtctgt gggactgcc ccaagtata tacacaaga gtttcacac tacgtccag tggattgaa gatatagtt agttattta ttactgtca cataaagcc aaaatggtt gacgctaca aaaaggcct cttcattta cccccttgg cacatccct tatttggag aaacacaga atcctcgaa ttatttcat gaattagcc agagaactt gtgattcaa caaccggaa gaccacgta ttgtttcta aaacaggtc ttaatcaat gctgctaga aacagagac acggcgaga gttgtcata atttgctcc ccaaaagtg aactgttta gaaattgca aatgcgatt gctgaaata accaaacag aacgttata actgaacat tccctactt acttttagc ggacgtatg gaaatacaa agccccgac ttgagggcc aaatacatt ggtcatttg gttcgaacc gataatgcg tacgaaaat ggctggata ctagtcgat tgtatcaaa aacgaaaac gatgcgaaa gctctccta aagctcggg attcttcca actcatgtt atccacctt attccttcc tttcagcct cctttgaca gaattactg tactgccat gttcctgca gaatggccc caatacaga cgcactgtt cttggatta cgaaacgcc ttcaaacat gttttacga gaagtgttt ttccaacag cgcggctta caagaaatc gtagcagaa tgcatagaa attctaaac atcaaaccg gcaaaatat gccatcaaa cccagagtt ttgattttg gggcctcga gggtcaggc aggaagaca caggccaag ttactagcc caggggctc ggtcttatc cacgttgat tttgaacat ttgatctgc gaggcatgg atgtcagac tccgacttg ggaaaaagg ctccgcaac tgcaaaaac gaggtctgt tttcactcc gaacttctt gctgaagta gtacataag cgagttttg gaagacgat tgtttggaa cacggctgg gttttaacg ggatttccg ttctctaaa cgagatttt gaatacttg gactgtatt tatacaccc ccaaacagg gtaatattt ctcgaatgc gacctcgcg gtttgcaaa gagcgcctc ctgaaccga aaaattaac gtcgacacc ggttcagtc acaaacgtt aaagaacac ccagaggcc gaaatgagc aaacaatta aaaacacat ccaaaagac aacaaagaa tttatcgat gccgagctt tcctactac tgtgagaat tacggcgat ttgaaaaaa tattgtggt gcaagtgct gtttgttta aatggggat caggccgag cgttggatt tacgaaaca attgttgcc atcatatca cgggcgcca ccacctcac aacccccgc agacctctt ctggacctg tccagcagt tccgtgtcc ttggacgcc tgttgttgc tccccaatt ccgtcacaa atcctggac tgttacaac acaagactg tga
- >TC032066 RA
actaacctt gcaagttta tgcgccgtt attcagcca tgattttag taaacattt agttttaac ttgattcaa attaaattt tcggttaca atgtggtgg acccgttca acggtaaca acagtagtt ctactaact cttataaag ccttttcac tgtcaggtt gtcggtatt tcttgctct gtaaaaaat tccgatgac ttgggagtt tgcaaactg ttaagtgaa tgtaagcaa gtccgtgac gacatttta aaacaacaa caactacca caactatgc ggatttcgg gaaactcaa gcaatagtt tgttgtcca cagaaaaag cgaaaaccg cgtgacatt agcaaaata aaatgtaag gagtattct tcttatgcg aaaaacgaa tgcggacat aaaattgtt aaacgtatt ataggcgga gaatttgca gggagcaaa gaatttccg cacatggca ttattagga tttgaaaaa aaaggttca gctaataac ataacacaa tggagatgt gacggtata attattagc gaccgctac attttaact gtggcgcag tgtttaaat acgaggggt tttgatctt aaatatgta aaagtgggg gtcactgat cttaacgaa actgaccat caacaagaa ataagagtg ttacaaacc gttccacat cctgactat aaacctctt caaagtctc aggaatgat attggactt attaaatta gaaaaaccg atcgagtta aattcttat gcacgaccg gcttgtctc tacacagag agagcactt gcagttgat ttggctgta gtcactgga tggggtgtt acagaagca ggatctagc agcaataaa ttaaaaaaa gtagaacta ccttttgtg tccagtgaa ttgtgtgcc gtcttattt aaatatgct aaaaatggg ttcgatgaa gatttacaa atttgtgct ggaaccgca aataaggac agttgtcag ggcgacaca ggaggccca cttcagata catcatgaa ggtgatgat atttcctgc atgtatgat attgtggga atttcttct gctggggct agggtctgt gggactgcc ccaagtata tacacaaga gtttcacac tacgtccag tggattgaa gatatagtt agttattta ttactgtca cataaagcc aaaatggtt gacgctaca aaaaggcct cttcattta cccccttgg cacatccct tatttggag aaacacaga atcctcgaa ttatttcat gaattagcc agagaactt gtgattcaa caaccggaa gaccacgta ttgtttcta aaacaggtc ttaatcaat gctgctaga aacagagac acggcgaga gttgtcata atttgctcc ccaaaagtg aactgttta gaaattgca aatgcgatt gctgaaata accaaacag aacgttata actgaacat tccctactt acttttagc ggacgtatg gaaatacaa agccccgac ttgagggcc aaatacatt ggtcatttg gttcgaacc gataatgcg tacgaaaat ggctggata ctagtcgat tgtatcaaa aacgaaaac gatgcgaaa gctctccta aagctcggg attcttcca actcatgtt atccacctt attccttcc tttcagcct cctttgaca gaattactg tactgccat gttcctgca gaatggccc caatacaga cgcactgtt cttggatta cgaaacgcc ttcaaacat gttttacga gaagtgttt ttccaacag cgcggctta caagaaatc gtagcagaa tgcatagaa attctaaac atcaaaccg gcaaaatat gccatcaaa cccagagtt ttgattttg gggcctcga gggtcaggc aggaagaca caggccaag ttactagcc caggggctc ggtcttatc cacgttgat tttgaacat ttgatctgc gaggcatgg atgtcagac tccgacttg ggaaaaagg ctccgcaac tgcaaaaac gaggtctgt tttcactcc gaacttctt gctgaagta gtacataag cgagttttg gaagacgat tgtttggaa cacggctgg gttttaacg ggatttccg ttctctaaa cgagatttt gaatacttg gactgtatt tatacaccc ccaaacagg gtaatattt ctcgaatgc gacctcgcg gtttgcaaa gagcgcctc ctgaaccga aaaattaac gtcgacacc ggttcagtc acaaacgtt aaagaacac ccagaggcc gaaatgagc aaacaatta aaaacacat ccaaaagac aacaaagaa tttatcgat gccgagctt tcctactac tgtgagaat tacggcgat ttgaaaaaa tattgtggt gcaagtgct gtttgttta aatggggat caggccgag cgttggatt tacgaaaca attgttgcc atcatatca cgggcgcca ccacctcac aacccccgc agacctctt ctggacctg tccagcagt tccgtgtcc ttggacgcc tgttgttgc tccccaatt ccgtcacaa atcctggac tgttacaac acaagactg tgaaataaa agcgccaat gcagt