Status
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Gene information
General information
TC032066
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000740,TC000741
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 949916..954666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032066
    iBeetle screen iB_03068
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032066 RA
      MWWTRSTVT TVVLLTLIK PFHCQVVGI SCSVKNSDD 
      LGVCKLLSE CKQVRDDIL KQQQLPQLC GFRETQAIV 
      CCPQKKRKP RDISKIKCK EYSSYAKNE CGHKIVKRI 
      IGGEFAGSK EFPHMALLG FEKKGSANN ITQWRCDGI 
      IISDRYILT VAQCLNTRG FDLKYVKVG VTDLNETDH 
      QQEIRVLQT VPHPDYKPL QSLRNDIGL IKLEKPIEL 
      NSYARPACL YTERALAVD LAVVTGWGV TEAGSSSNK 
      LKKVELPFV SSELCAVLF KYAKNGFDE DLQICAGTA 
      NKDSCQGDT GGPLQIHHE GDDISCMYD IVGISSAGA 
      RVCGTAPSI YTRVSHYVQ WIEDIVSYL LLSHKAKMV 
      DATKRPLHL PPWHIPYLE KHRILELFH ELARELVIQ 
      QPEDHVLFL KQVLINAAR NRDTARVVI ICSPKVNCL 
      EIANAIAEI TKQNVITEH SLLTFSGRM EIQSPDLRA 
      KYIGHLVRT DNAYENGWI LVDCIKNEN DAKALLKLG 
      ILPTHVIHL IPSFQPPLT ELLYCHVPA EWPQYRRTV 
      LGLRNAFKH VLREVFFQQ RGLQEIVAE CIEILNIKP 
      AKYAIKPRV LILGPRGSG RKTQAKLLA QGLGLIHVD 
      FEHLICEAW MSDSDLGKR LRNCKNEVC FHSELLAEV 
      VHKRVLEDD CLEHGWVLT GFPFSKRDF EYLDCIYTP 
      PNRVIFLEC DLAVCKERL LNRKINVDT GSVTNVKEH 
      PEAEMSKQL KTHPKDNKE FIDAELSYY CENYGDLKK 
      YCGASAVCL NGDQAERWI YETIVAIIS RAPPPHNPR 
      RPLLDLSSS SVSLDACCC SPIPSQILD CYNTRL
    CDS sequence
    • >TC032066 RA
      atgtggtgg acccgttca acggtaaca acagtagtt 
      ctactaact cttataaag ccttttcac tgtcaggtt 
      gtcggtatt tcttgctct gtaaaaaat tccgatgac 
      ttgggagtt tgcaaactg ttaagtgaa tgtaagcaa 
      gtccgtgac gacatttta aaacaacaa caactacca 
      caactatgc ggatttcgg gaaactcaa gcaatagtt 
      tgttgtcca cagaaaaag cgaaaaccg cgtgacatt 
      agcaaaata aaatgtaag gagtattct tcttatgcg 
      aaaaacgaa tgcggacat aaaattgtt aaacgtatt 
      ataggcgga gaatttgca gggagcaaa gaatttccg 
      cacatggca ttattagga tttgaaaaa aaaggttca 
      gctaataac ataacacaa tggagatgt gacggtata 
      attattagc gaccgctac attttaact gtggcgcag 
      tgtttaaat acgaggggt tttgatctt aaatatgta 
      aaagtgggg gtcactgat cttaacgaa actgaccat 
      caacaagaa ataagagtg ttacaaacc gttccacat 
      cctgactat aaacctctt caaagtctc aggaatgat 
      attggactt attaaatta gaaaaaccg atcgagtta 
      aattcttat gcacgaccg gcttgtctc tacacagag 
      agagcactt gcagttgat ttggctgta gtcactgga 
      tggggtgtt acagaagca ggatctagc agcaataaa 
      ttaaaaaaa gtagaacta ccttttgtg tccagtgaa 
      ttgtgtgcc gtcttattt aaatatgct aaaaatggg 
      ttcgatgaa gatttacaa atttgtgct ggaaccgca 
      aataaggac agttgtcag ggcgacaca ggaggccca 
      cttcagata catcatgaa ggtgatgat atttcctgc 
      atgtatgat attgtggga atttcttct gctggggct 
      agggtctgt gggactgcc ccaagtata tacacaaga 
      gtttcacac tacgtccag tggattgaa gatatagtt 
      agttattta ttactgtca cataaagcc aaaatggtt 
      gacgctaca aaaaggcct cttcattta cccccttgg 
      cacatccct tatttggag aaacacaga atcctcgaa 
      ttatttcat gaattagcc agagaactt gtgattcaa 
      caaccggaa gaccacgta ttgtttcta aaacaggtc 
      ttaatcaat gctgctaga aacagagac acggcgaga 
      gttgtcata atttgctcc ccaaaagtg aactgttta 
      gaaattgca aatgcgatt gctgaaata accaaacag 
      aacgttata actgaacat tccctactt acttttagc 
      ggacgtatg gaaatacaa agccccgac ttgagggcc 
      aaatacatt ggtcatttg gttcgaacc gataatgcg 
      tacgaaaat ggctggata ctagtcgat tgtatcaaa 
      aacgaaaac gatgcgaaa gctctccta aagctcggg 
      attcttcca actcatgtt atccacctt attccttcc 
      tttcagcct cctttgaca gaattactg tactgccat 
      gttcctgca gaatggccc caatacaga cgcactgtt 
      cttggatta cgaaacgcc ttcaaacat gttttacga 
      gaagtgttt ttccaacag cgcggctta caagaaatc 
      gtagcagaa tgcatagaa attctaaac atcaaaccg 
      gcaaaatat gccatcaaa cccagagtt ttgattttg 
      gggcctcga gggtcaggc aggaagaca caggccaag 
      ttactagcc caggggctc ggtcttatc cacgttgat 
      tttgaacat ttgatctgc gaggcatgg atgtcagac 
      tccgacttg ggaaaaagg ctccgcaac tgcaaaaac 
      gaggtctgt tttcactcc gaacttctt gctgaagta 
      gtacataag cgagttttg gaagacgat tgtttggaa 
      cacggctgg gttttaacg ggatttccg ttctctaaa 
      cgagatttt gaatacttg gactgtatt tatacaccc 
      ccaaacagg gtaatattt ctcgaatgc gacctcgcg 
      gtttgcaaa gagcgcctc ctgaaccga aaaattaac 
      gtcgacacc ggttcagtc acaaacgtt aaagaacac 
      ccagaggcc gaaatgagc aaacaatta aaaacacat 
      ccaaaagac aacaaagaa tttatcgat gccgagctt 
      tcctactac tgtgagaat tacggcgat ttgaaaaaa 
      tattgtggt gcaagtgct gtttgttta aatggggat 
      caggccgag cgttggatt tacgaaaca attgttgcc 
      atcatatca cgggcgcca ccacctcac aacccccgc 
      agacctctt ctggacctg tccagcagt tccgtgtcc 
      ttggacgcc tgttgttgc tccccaatt ccgtcacaa 
      atcctggac tgttacaac acaagactg tga
    mRNA sequence
    • >TC032066 RA
      actaacctt gcaagttta tgcgccgtt attcagcca 
      tgattttag taaacattt agttttaac ttgattcaa 
      attaaattt tcggttaca atgtggtgg acccgttca 
      acggtaaca acagtagtt ctactaact cttataaag 
      ccttttcac tgtcaggtt gtcggtatt tcttgctct 
      gtaaaaaat tccgatgac ttgggagtt tgcaaactg 
      ttaagtgaa tgtaagcaa gtccgtgac gacatttta 
      aaacaacaa caactacca caactatgc ggatttcgg 
      gaaactcaa gcaatagtt tgttgtcca cagaaaaag 
      cgaaaaccg cgtgacatt agcaaaata aaatgtaag 
      gagtattct tcttatgcg aaaaacgaa tgcggacat 
      aaaattgtt aaacgtatt ataggcgga gaatttgca 
      gggagcaaa gaatttccg cacatggca ttattagga 
      tttgaaaaa aaaggttca gctaataac ataacacaa 
      tggagatgt gacggtata attattagc gaccgctac 
      attttaact gtggcgcag tgtttaaat acgaggggt 
      tttgatctt aaatatgta aaagtgggg gtcactgat 
      cttaacgaa actgaccat caacaagaa ataagagtg 
      ttacaaacc gttccacat cctgactat aaacctctt 
      caaagtctc aggaatgat attggactt attaaatta 
      gaaaaaccg atcgagtta aattcttat gcacgaccg 
      gcttgtctc tacacagag agagcactt gcagttgat 
      ttggctgta gtcactgga tggggtgtt acagaagca 
      ggatctagc agcaataaa ttaaaaaaa gtagaacta 
      ccttttgtg tccagtgaa ttgtgtgcc gtcttattt 
      aaatatgct aaaaatggg ttcgatgaa gatttacaa 
      atttgtgct ggaaccgca aataaggac agttgtcag 
      ggcgacaca ggaggccca cttcagata catcatgaa 
      ggtgatgat atttcctgc atgtatgat attgtggga 
      atttcttct gctggggct agggtctgt gggactgcc 
      ccaagtata tacacaaga gtttcacac tacgtccag 
      tggattgaa gatatagtt agttattta ttactgtca 
      cataaagcc aaaatggtt gacgctaca aaaaggcct 
      cttcattta cccccttgg cacatccct tatttggag 
      aaacacaga atcctcgaa ttatttcat gaattagcc 
      agagaactt gtgattcaa caaccggaa gaccacgta 
      ttgtttcta aaacaggtc ttaatcaat gctgctaga 
      aacagagac acggcgaga gttgtcata atttgctcc 
      ccaaaagtg aactgttta gaaattgca aatgcgatt 
      gctgaaata accaaacag aacgttata actgaacat 
      tccctactt acttttagc ggacgtatg gaaatacaa 
      agccccgac ttgagggcc aaatacatt ggtcatttg 
      gttcgaacc gataatgcg tacgaaaat ggctggata 
      ctagtcgat tgtatcaaa aacgaaaac gatgcgaaa 
      gctctccta aagctcggg attcttcca actcatgtt 
      atccacctt attccttcc tttcagcct cctttgaca 
      gaattactg tactgccat gttcctgca gaatggccc 
      caatacaga cgcactgtt cttggatta cgaaacgcc 
      ttcaaacat gttttacga gaagtgttt ttccaacag 
      cgcggctta caagaaatc gtagcagaa tgcatagaa 
      attctaaac atcaaaccg gcaaaatat gccatcaaa 
      cccagagtt ttgattttg gggcctcga gggtcaggc 
      aggaagaca caggccaag ttactagcc caggggctc 
      ggtcttatc cacgttgat tttgaacat ttgatctgc 
      gaggcatgg atgtcagac tccgacttg ggaaaaagg 
      ctccgcaac tgcaaaaac gaggtctgt tttcactcc 
      gaacttctt gctgaagta gtacataag cgagttttg 
      gaagacgat tgtttggaa cacggctgg gttttaacg 
      ggatttccg ttctctaaa cgagatttt gaatacttg 
      gactgtatt tatacaccc ccaaacagg gtaatattt 
      ctcgaatgc gacctcgcg gtttgcaaa gagcgcctc 
      ctgaaccga aaaattaac gtcgacacc ggttcagtc 
      acaaacgtt aaagaacac ccagaggcc gaaatgagc 
      aaacaatta aaaacacat ccaaaagac aacaaagaa 
      tttatcgat gccgagctt tcctactac tgtgagaat 
      tacggcgat ttgaaaaaa tattgtggt gcaagtgct 
      gtttgttta aatggggat caggccgag cgttggatt 
      tacgaaaca attgttgcc atcatatca cgggcgcca 
      ccacctcac aacccccgc agacctctt ctggacctg 
      tccagcagt tccgtgtcc ttggacgcc tgttgttgc 
      tccccaatt ccgtcacaa atcctggac tgttacaac 
      acaagactg tgaaataaa agcgccaat gcagt