Status
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Gene information
General information
TC032073
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000730,TC000731,TC030041
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6701490..6711157
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032073
    iBeetle screen iB_03065

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032073 RA
      MYSTLFVGS TFVLTALSQ IANMINSFG DMERMTEAS 
      FILFTNVVQ CFKIYSFLT YGPRVWNLI DGLNRNIFK 
      PINTDQHRI LVNDIYMSK KISKIFLLA CTLTCMSWA 
      ISPFFDKRG DVLRLPLSG WYPFNTDKS PAFELVYIY 
      QILTTWIGG MGNISMDTF ISGIIMAIS SQLSILNNA 
      LKNITKNNE LVRCVFHYR IIIKFSDEV IYLFNTCLT 
      TQFIVGVII VCISMFQMS LVPVLSFQF VAMLLYQMC 
      ILLEIFLWC FYGNEVMLK SDQLTQAAY MSDWTKSPN 
      HFKQNLLFF MTRTQFPLK LYASGYFTL SLETFKAIV 
      KSSWSYFAV LNQVHSRQT QNNSQRKLL FRIYAALFV 
      GFAFILSLL SQIANMVDA FGDIEKMTE ASFLLFTNL 
      VQCFKIYTF ANHGKKVWN LVYSMNRSD FKPNNLAQY 
      WIVVNEIKT SKIISKLFL LACTLTCVS WAISPLLDK 
      RGSDELRLP LSGWYPFST EKSPAFEFA YTYQIFTTW 
      VGGLGDISM DTFMSGTIM VISTQLSLL KDGLENVAR 
      NIKHDKSSV NKNLIQCAC HYRSIIQFA AEVTNLFTT 
      CITAQFVVG VIIVCMSMF QMSLVSVLS FQFAAMLLY 
      QICVLMEIY LWCFYGNEV MLKSDQLTQ AAYMSEWLD 
      GTEEFKQNL LFLMTRTQF PLKLYASGY FTLSLETFK 
      AIVKSSWSY FAVLNRTVG ALVNGLGNI SIDTFLSGI 
      IMVVSGQLK ILNNSLQTM KPNCKANDT ERRQKLIDI 
      IIHHRSIIQ FAQEMTWLF TTCIMSQFV VSVIIICIT 
      MFQMSLVSP LSLQFLSMA LYQACMITE IFLWCYYGN 
      EVILQSGKL TQSAYMSQW LSSSKKFKH DLMFFMTRS 
      QNPLKLYAG GYFTLSLET FMAIVKSSW SYFAVLNRV 
      HTTDNV
    CDS sequence
    • >TC032073 RA
      atgtattct actctgttt gtgggctca acttttgtt 
      ttaactgct ctgagtcaa attgccaac atgattaat 
      tctttcggc gacatggaa agaatgaca gaagcctca 
      tttatactt ttcactaac gtggtacaa tgtttcaaa 
      atatattcg ttcctaacg tacggacca agagtgtgg 
      aacttgatt gatggtttg aacagaaac atttttaag 
      ccgataaat acggaccaa caccggata ttagtaaac 
      gacatctac atgtcgaaa aagatttca aaaatattt 
      ctactggct tgcaccctt acttgcatg tcatgggcg 
      atttccccg ttttttgat aagcggggt gatgtcctc 
      agattgccc ttgagtggt tggtaccct ttcaacact 
      gataaatct ccagcattt gagcttgtc tacatttat 
      caaatttta accacttgg attggagga atgggcaac 
      atcagtatg gacaccttc atctccgga attataatg 
      gccatttcc tcacaactc agtattcta aacaatgct 
      ctcaaaaat ataacgaaa aataatgaa ttagtccgt 
      tgcgttttt cattacaga attattatt aagttttca 
      gatgaagta atttatctt ttcaatact tgcctcaca 
      actcagttt atagtcgga gtgatcatt gtgtgtatc 
      tcaatgttt caaatgagt ttggttcca gttctcagt 
      ttccaattt gttgccatg ctcctttat caaatgtgt 
      attctgcta gaaatattc ttgtggtgt ttttacgga 
      aacgaagta atgttgaag agtgatcag ttaacacaa 
      gcagcctat atgagcgac tggaccaaa agcccaaat 
      cattttaaa caaaatttg ttgtttttt atgacgcgt 
      acacagttt ccactaaaa ctgtatgct tcagggtat 
      tttactcta tctctcgag actttcaaa gctattgtg 
      aaatcgtct tggtcttat tttgctgtt ttgaatcag 
      gtccattct cgacaaacg cagaataat tctcaaagg 
      aaattactt tttcgcatc tacgctgcc ctttttgtc 
      ggatttgcg ttcattttg tcgctcttg agccaaatt 
      gccaacatg gtagatgcg tttggtgac atcgaaaaa 
      atgacagaa gcctcattt ctgcttttt acaaatctg 
      gtccaatgt ttcaagatt tatactttt gcgaaccac 
      ggcaaaaaa gtatggaat ttggtgtac agcatgaac 
      agaagtgat tttaagccg aacaatttg gcccagtac 
      tggattgtg gtcaatgag ataaaaaca tcgaaaata 
      atttcgaaa ttattttta ctcgcatgt acgttgact 
      tgtgtctct tgggccatt tcgccactt ttggacaag 
      agaggtagt gatgagctg agactaccg ttaagtggc 
      tggtatccg tttagcacg gaaaaatcg ccagctttc 
      gaatttgct tacacttat caaattttt accacttgg 
      gttggggga cttggagac ataagtatg gacactttt 
      atgtcaggc accatcatg gttatttcg acacagctc 
      agtttactg aaagatgga cttgaaaat gttgcaagg 
      aacatcaaa catgacaaa agttcggtt aataaaaat 
      ttaattcaa tgtgcttgt cattacaga agtattata 
      cagtttgcc gctgaagta actaatctt ttcacaacc 
      tgtataacg gcccagttc gtagtagga gtaataatt 
      gtttgcatg tccatgttt caaatgagc ctagtttcg 
      gtgctaagt ttccaattt gccgcaatg ttattgtac 
      caaatttgt gtcctcatg gagatttat ttgtggtgt 
      ttttacggc aatgaagtg atgcttaag agtgatcaa 
      cttacccag gcggcttat atgtcggaa tggcttgat 
      ggtacagaa gaattcaag caaaatcta cttttcttg 
      atgacacgg actcaattc cctcttaaa ttgtatgct 
      tctgggtat tttacacta tctcttgaa acttttaaa 
      gcgattgtt aaatcatct tggtcgtat tttgcagtt 
      ttaaaccgg actgtaggt gcgttggtt aatggtttg 
      gggaatatt agcatagat acgtttcta tccgggatt 
      ataatggtt gtttctgga cagttaaaa atattaaac 
      aattcgctc caaaccatg aaaccaaac tgcaaagca 
      aatgacaca gaaaggcgc cagaaatta attgacatc 
      attattcat catcgaagt ataatacag tttgcccaa 
      gaaatgacc tggcttttc actacctgt atcatgagt 
      cagtttgtg gtcagtgta ataataatt tgcatcaca 
      atgttccaa atgagtttg gtgtcaccg ttgagtttg 
      caatttttg tcaatggcc ctatatcaa gcctgtatg 
      atcactgag atatttctg tggtgttat tacggcaac 
      gaagtaata ctacagagt ggcaaactc acacagtcg 
      gcttatatg agccagtgg ttgagttcc tccaaaaag 
      tttaaacac gatttgatg ttctttatg actcgttcg 
      caaaatcca ctcaaactt tacgctgga ggttatttt 
      acattgtct ttagagaca tttatggct attgtaaag 
      tcgagttgg tcgtatttt gcggtttta aacagagtg 
      cataccaca gataacgta tag
    mRNA sequence
    • >TC032073 RA
      aagtaattt accgtaaaa actgattgt caattaata 
      aattatcca acaatttga ggcgtcttg cattataga 
      tgactgccc taaaggaaa atgaaaata aattactgc 
      tttcaggtc tacaaaatt gacaaaacg tactattag 
      taattttaa ttatagttg ccatctaaa ctaaatttt 
      aaattagat gaaattata ccgatctaa tcatttctt 
      acaaagtta atcagtcaa acagtttag acacaatga 
      gtaaccagc acgaaatag acttaacag aatttgtta 
      aacttaaca tcaaaaata tgcactttt ttgggtatt 
      ttttcccga gatttggac acaataaga cgagaaaaa 
      cactttaca ccatgtatt ctactctgt ttgtgggct 
      caacttttg ttttaactg ctctgagtc aaattgcca 
      acatgatta attctttcg gcgacatgg aaagaatga 
      cagaagcct catttatac ttttcacta acgtggtac 
      aatgtttca aaatatatt cgttcctaa cgtacggac 
      caagagtgt ggaacttga ttgatggtt tgaacagaa 
      acattttta agccgataa atacggacc aacaccgga 
      tattagtaa acgacatct acatgtcga aaaagattt 
      caaaaatat ttctactgg cttgcaccc ttacttgca 
      tgtcatggg cgatttccc cgttttttg ataagcggg 
      gtgatgtcc tcagattgc ccttgagtg gttggtacc 
      ctttcaaca ctgataaat ctccagcat ttgagcttg 
      tctacattt atcaaattt taaccactt ggattggag 
      gaatgggca acatcagta tggacacct tcatctccg 
      gaattataa tggccattt cctcacaac tcagtattc 
      taaacaatg ctctcaaaa atataacga aaaataatg 
      aattagtcc gttgcgttt ttcattaca gaattatta 
      ttaagtttt cagatgaag taatttatc ttttcaata 
      cttgcctca caactcagt ttatagtcg gagtgatca 
      ttgtgtgta tctcaatgt ttcaaatga gtttggttc 
      cagttctca gtttccaat ttgttgcca tgctccttt 
      atcaaatgt gtattctgc tagaaatat tcttgtggt 
      gtttttacg gaaacgaag taatgttga agagtgatc 
      agttaacac aagcagcct atatgagcg actggacca 
      aaagcccaa atcatttta aacaaaatt tgttgtttt 
      ttatgacgc gtacacagt ttccactaa aactgtatg 
      cttcagggt attttactc tatctctcg agactttca 
      aagctattg tgaaatcgt cttggtctt attttgctg 
      ttttgaatc aggtccatt ctcgacaaa cgcagaata 
      attctcaaa ggaaattac tttttcgca tctacgctg 
      ccctttttg tcggatttg cgttcattt tgtcgctct 
      tgagccaaa ttgccaaca tggtagatg cgtttggtg 
      acatcgaaa aaatgacag aagcctcat ttctgcttt 
      ttacaaatc tggtccaat gtttcaaga tttatactt 
      ttgcgaacc acggcaaaa aagtatgga atttggtgt 
      acagcatga acagaagtg attttaagc cgaacaatt 
      tggcccagt actggattg tggtcaatg agataaaaa 
      catcgaaaa taatttcga aattatttt tactcgcat 
      gtacgttga cttgtgtct cttgggcca tttcgccac 
      ttttggaca agagaggta gtgatgagc tgagactac 
      cgttaagtg gctggtatc cgtttagca cggaaaaat 
      cgccagctt tcgaatttg cttacactt atcaaattt 
      ttaccactt gggttgggg gacttggag acataagta 
      tggacactt ttatgtcag gcaccatca tggttattt 
      cgacacagc tcagtttac tgaaagatg gacttgaaa 
      atgttgcaa ggaacatca aacatgaca aaagttcgg 
      ttaataaaa atttaattc aatgtgctt gtcattaca 
      gaagtatta tacagtttg ccgctgaag taactaatc 
      ttttcacaa cctgtataa cggcccagt tcgtagtag 
      gagtaataa ttgtttgca tgtccatgt ttcaaatga 
      gcctagttt cggtgctaa gtttccaat ttgccgcaa 
      tgttattgt accaaattt gtgtcctca tggagattt 
      atttgtggt gtttttacg gcaatgaag tgatgctta 
      agagtgatc aacttaccc aggcggctt atatgtcgg 
      aatggcttg atggtacag aagaattca agcaaaatc 
      tacttttct tgatgacac ggactcaat tccctctta 
      aattgtatg cttctgggt attttacac tatctcttg 
      aaactttta aagcgattg ttaaatcat cttggtcgt 
      attttgcag ttttaaacc ggactgtag gtgcgttgg 
      ttaatggtt tggggaata ttagcatag atacgtttc 
      tatccggga ttataatgg ttgtttctg gacagttaa 
      aaatattaa acaattcgc tccaaacca tgaaaccaa 
      actgcaaag caaatgaca cagaaaggc gccagaaat 
      taattgaca tcattattc atcatcgaa gtataatac 
      agtttgccc aagaaatga cctggcttt tcactacct 
      gtatcatga gtcagtttg tggtcagtg taataataa 
      tttgcatca caatgttcc aaatgagtt tggtgtcac 
      cgttgagtt tgcaatttt tgtcaatgg ccctatatc 
      aagcctgta tgatcactg agatatttc tgtggtgtt 
      attacggca acgaagtaa tactacaga gtggcaaac 
      tcacacagt cggcttata tgagccagt ggttgagtt 
      cctccaaaa agtttaaac acgatttga tgttcttta 
      tgactcgtt cgcaaaatc cactcaaac tttacgctg 
      gaggttatt ttacattgt ctttagaga catttatgg 
      ctattgtaa agtcgagtt ggtcgtatt ttgcggttt 
      taaacagag tgcatacca cagataacg tatagtgaa 
      tggccaaaa atggaaatt attgaaatt ctcagttga 
      ttaacgtgt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY