Status
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Gene information
General information
TC030991
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000712,TC000713
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6990588..7003463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030991
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03064

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen musculature random irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: App. 35% are affected.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum potentially position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030991 RA
      MGTEFKDVD VNMEDSGNS CGSSVVSDY SGPSDHTDF 
      DGDGVQVSD LEGDSDELR LTQCSTPDA YGDCMADVN 
      YFLEMEERM KSNNSSVGD VKNIESQQR LAVLSFEQV 
      CRLNDVMDE VVSIHGRGN FPTLEVRLR DLVNVVRKK 
      LETDTASGG AGMRVRDIR LNGGAASHV LATESQPYN 
      DLDLIFGVD LSNVRNFDR VKSAVLSSL YEMLPDGVS 
      RKRMSTCSL KEAYVSKMV KVNSSNNDG DRWSLISLG 
      NSRGRGVEL KFVDSMRRQ FEFSVDSFQ IILDSLLLF 
      YECSELPIS ENFYPTVVG ESFYGDFQE ALYHLHKKL 
      ISTRHPEEI RGGGLLKYC NLLVKNYKP ASPDYIKSL 
      QKYMCSRFF IDFPDINQQ RGKLENYLW NHFVEPEEE 
      ALKHQYLML LHDVVEEST VCLMGHERR QTLQLIKSL 
      AWQVLYQDQ QRIMTQQPP PLMYANGIV YAPIIQTSC 
      YCTCNTWMS CCSS
    CDS sequence
    • >TC030991 RA
      atgggcaca gaattcaag gatgttgac gtgaacatg 
      gaagactcc gggaatagc tgcggaagc agcgtcgtc 
      tccgactac agcggccct tcggaccac accgacttc 
      gacggcgat ggcgtccag gtctccgac ctcgaggga 
      gacagcgac gagctgcgg ctcacacag tgcagcaca 
      ccagacgcg tacggtgac tgcatggca gacgtgaac 
      tacttcctt gaaatggaa gagaggatg aagagtaat 
      aacagtagc gttggggat gttaagaat attgaatct 
      cagcaacgg ctggcagtg ctcagtttc gagcaggtc 
      tgccggttg aacgatgtg atggatgag gtggtgagc 
      atccacgga cgtggcaac ttcccgact ctggaggtc 
      cggctcaga gacctggtg aacgtggtg aggaagaag 
      ctggagact gataccgcg tcgggcggc gcgggcatg 
      cgagtccgc gatatccgt ctgaacggt ggcgcagct 
      tcgcacgtt ttggccacc gagtctcag ccatataac 
      gatttggat ctcatcttc ggagtcgat ttgtccaac 
      gtgcggaat ttcgaccgt gtcaagtcc gccgttctc 
      agttctctc tatgaaatg ttacctgat ggcgtcagc 
      cgcaaacgc atgtccaca tgcagtctc aaggaagct 
      tacgtaagc aagatggtg aaagtgaat agtagtaat 
      aatgatggt gatcgctgg tccctcatc tctctcggt 
      aattcccgc ggtcgtggc gtcgaattg aaattcgtc 
      gacagcatg cgacggcag ttcgagttc tccgtcgat 
      tcgtttcaa ataatcttg gactctctt ttgctcttc 
      tacgaatgc agcgagctt cccatcagc gagaatttc 
      tacccgacg gtcgtcggc gaatccttc tacggcgac 
      ttccaggaa gctttgtac cacttacac aagaagctg 
      atctcaacg agacacccc gaggaaata cgcgggggc 
      ggtctgctc aagtattgc aatttgcta gtcaagaac 
      tacaaaccg gcgagtccc gattacatc aagagtctc 
      caaaagtac atgtgctcg cggtttttc atcgatttc 
      cccgacatt aaccaacag agggggaaa ctagagaac 
      tacctttgg aaccacttt gtcgagccg gaggaagag 
      gctctcaaa caccagtac ctgatgcta ttgcacgac 
      gtggtggag gagtcgacc gtttgtctg atgggtcac 
      gaacggcga caaaccctc cagctgata aagagtctg 
      gcttggcag gtcctctac caggaccag cagcgcata 
      atgacccag cagccgccg cccctgatg tacgccaac 
      ggcatcgtc tacgctccc atcatccag acgagctgc 
      tattgtacg tgcaacaca tggatgtcg tgctgttcg 
      tcgtga
    mRNA sequence
    • >TC030991 RA
      tatcctggt ggatttgaa tgcgcagcc gccaggttg 
      gagtatcgc gagttgagt ccgctgtcg gaggctctt 
      cccagtgcg gcgtgtgat agtgtaacg tgtggtagg 
      ccacggtga agtcttatt agcaaccaa cttgcattt 
      tcatcaagc gtgaaattg tgcagtgcc gtcctagaa 
      tcacacatt ccttgtgct gaccgccgg tcacagttg 
      tagttgttg tgagcatgg gcacagaat tcaaggatg 
      ttgacgtga acatggaag actccggga atagctgcg 
      gaagcagcg tcgtctccg actacagcg gcccttcgg 
      accacaccg acttcgacg gcgatggcg tccaggtct 
      ccgacctcg agggagaca gcgacgagc tgcggctca 
      cacagtgca gcacaccag acgcgtacg gtgactgca 
      tggcagacg tgaactact tccttgaaa tggaagaga 
      ggatgaaga gtaataaca gtagcgttg gggatgtta 
      agaatattg aatctcagc aacggctgg cagtgctca 
      gtttcgagc aggtctgcc ggttgaacg atgtgatgg 
      atgaggtgg tgagcatcc acggacgtg gcaacttcc 
      cgactctgg aggtccggc tcagagacc tggtgaacg 
      tggtgagga agaagctgg agactgata ccgcgtcgg 
      gcggcgcgg gcatgcgag tccgcgata tccgtctga 
      acggtggcg cagcttcgc acgttttgg ccaccgagt 
      ctcagccat ataacgatt tggatctca tcttcggag 
      tcgatttgt ccaacgtgc ggaatttcg accgtgtca 
      agtccgccg ttctcagtt ctctctatg aaatgttac 
      ctgatggcg tcagccgca aacgcatgt ccacatgca 
      gtctcaagg aagcttacg taagcaaga tggtgaaag 
      tgaatagta gtaataatg atggtgatc gctggtccc 
      tcatctctc tcggtaatt cccgcggtc gtggcgtcg 
      aattgaaat tcgtcgaca gcatgcgac ggcagttcg 
      agttctccg tcgattcgt ttcaaataa tcttggact 
      ctcttttgc tcttctacg aatgcagcg agcttccca 
      tcagcgaga atttctacc cgacggtcg tcggcgaat 
      ccttctacg gcgacttcc aggaagctt tgtaccact 
      tacacaaga agctgatct caacgagac accccgagg 
      aaatacgcg ggggcggtc tgctcaagt attgcaatt 
      tgctagtca agaactaca aaccggcga gtcccgatt 
      acatcaaga gtctccaaa agtacatgt gctcgcggt 
      ttttcatcg atttccccg acattaacc aacagaggg 
      ggaaactag agaactacc tttggaacc actttgtcg 
      agccggagg aagaggctc tcaaacacc agtacctga 
      tgctattgc acgacgtgg tggaggagt cgaccgttt 
      gtctgatgg gtcacgaac ggcgacaaa ccctccagc 
      tgataaaga gtctggctt ggcaggtcc tctaccagg 
      accagcagc gcataatga cccagcagc cgccgcccc 
      tgatgtacg ccaacggca tcgtctacg ctcccatca 
      tccagacga gctgctatt gtacgtgca acacatgga 
      tgtcgtgct gttcgtcgt gatgtgtat aagtctgtg 
      cgagtcgtc cgaagcgga accacagca tcttatcta 
      tccgtctag attcggtag ctgttggta aagactctt 
      tattagact ttagtcatt ttgtttaca cctcgtaca 
      gacaaatta ctactgact gaggtttcc atttttgga 
      gcccgtatg aactctctg aacgcaggc ttcacgtgt 
      cgtctcttt gactacgaa accattccg aaattttac 
      acagaaggc tatttgttg atttcgttt gcaacatga 
      ggctgaaag ttcttaaca aaacatgtt gacgcatca 
      ttggtgtgt ggctgtgag aaacacatt tataattgg 
      gttgtgtac atggagatt tagcgattg gagcgtatt 
      tctcgagag atgcagcct gaagcgtcc gatacgtcg 
      ctcaatttt actcaatat tgtaagtca caaggaccc 
      tctcgggcg ctccgccgc cactcctta gccctctcc 
      tcctactct ggaactttg ccgtaatgc attttatac 
      gcatattat tatacgtac ataacaatt atagcaaca 
      cgtttgtac cactttatt caccgacat attttttct 
      tcctttgtt tgtattttt tatttgatg ctttaaaaa 
      accgtttat ttaggtttt atgaaagta atgttatat 
      tacgttact tattccttt acattattg tgtacttta 
      aatacttat tatctttaa tagtgtatt gatgtttaa 
      gtattgcat cgacattgg gcctcttat tattgtatt 
      ttttattgt ttgatgctt accagggac cttcaggct 
      gcatacata catgtaata ttttaagaa atcctgcaa 
      tcagtgcaa aacgtcgcc cgatcgcaa taagtatta 
      aacattgta cataatgta aaatacaag tcataattc 
      atattaagt gaatatttt cagaaatat gtatgtagt 
      ttgtcacaa gtaaattag caagtgtat gatagatca 
      aatcggatt tttattttt agatacttt agagaaaat 
      acttaaaaa aatgttaaa aactgagcc ataaacaaa 
      caaaaaaaa aaacgaatt tgtgaattg gcctaatta 
      atattgttg tatgctcag tctgactag tagttatca 
      tagactgct cggtgacct ctttaacat gcctacaag 
      tacttatgt cgcgagggt gactgactg aaaaattac 
      atacataat attgctgga tctttagga ttcgctcta 
      agctattat ctatcatag gaaacaaaa atgaaaaaa 
      aatacaaaa tgtctattt tgattgaaa tgtatgtat 
      gttataatg ggcttaatc tgtatattt ttattgttc 
      cataggccc agtaaggat aaactaaga aaaatgtaa 
      aaacccaat aaaaatgaa taaaatgtg taaacattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY