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Gene information
General information
TC000711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 1513209..1539169
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000711
    iBeetle screen iB_03063
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000711 RA
      MALNKICTL CLQQSRNEL RVLNSLINK RHHFVYTPD 
      VKSPVKGET QKMNMFQAI NNALDLALK QDESALIFG 
      EDVAFGGVF RCTMGLQSK YGPGRVFNT PLCEQGIVG 
      FAIGAANMG STAIAEIQF ADYTFPAFD QLVNEAAKM 
      RYRSGGQYD CGKLTVRAP CGAVGHGGL YHSQSPEAY 
      FAHTPGLKV VIPRGPIKA KGLLSACIR DPDPCIIFE 
      PKTLYRAAV EEVPVDDYV LPIGRADVL LEGNNVTLI 
      GWGTQVHVL LEVAQLAKK QLNVSCEVI DLVSILPWD 
      KSTVCQSVK KTKRVLVAH EAPLTGGFG AELAATIQE 
      ECFLHLEAP VVRVTGFDT PFPHVFEPF YLPDKWRCL 
      EAIPALTSS ANL
    CDS sequence
    • >TC000711 RA
      atggcttta aataaaatt tgtacgctg tgtttgcaa 
      caaagcaga aacgaactc agagtttta aatagtctg 
      atcaataaa agacaccat tttgtttac acgccagac 
      gtaaaatca cctgttaaa ggtgaaacc cagaaaatg 
      aacatgttc caagcgatc aacaacgcc ctagacctg 
      gcgttgaaa caggacgaa tcagcctta attttcggc 
      gaggacgtc gcctttggc ggtgtgttc cgctgcacg 
      atgggcttg cagtcaaag tacggtcct ggtcgcgta 
      ttcaacacc cccctgtgt gaacaggga attgttggt 
      tttgcgatc ggtgcagcc aacatgggt tcaacagca 
      atagcagaa attcaattc gccgattac acatttccg 
      gcattcgat cagctggtg aatgaagct gcgaaaatg 
      aggtacagg agtgggggg caatatgat tgcggaaaa 
      ttaactgtc agggcgccc tgcggagcc gtaggacat 
      ggagggctt taccattca caaagtcct gaggcgtat 
      ttcgctcat actccagga ttaaaggtc gtcatacca 
      cgcgggcca ataaaagcc aaaggcctg ctctcggcc 
      tgcatccga gacccggat ccttgcatt atcttcgag 
      cctaagacc ctatatagg gcagctgtg gaagaagtg 
      cctgtcgac gattacgtt ctcccgata ggaagagca 
      gacgttttg ctcgaaggc aataatgtg accttaata 
      ggatggggc actcaggtt cacgtgctg ctcgaggtg 
      gctcaattg gccaagaag caattgaac gtttcctgt 
      gaagtgatc gacttggtt tcgattttg ccgtgggat 
      aaaagcact gtctgtcag tctgtcaag aaaaccaag 
      cgagtttta gtcgcccac gaagcaccg ctaaccgga 
      ggattcggg gcggaattg gctgcaaca attcaagag 
      gagtgtttc ctgcatctt gaggcacct gttgtgcga 
      gtcacgggc ttcgacacg ccgttccct cacgttttc 
      gagcccttc tatctgcca gacaagtgg agatgtttg 
      gaggcgata cccgcactc acttcaagt gctaatctc 
      tga
    mRNA sequence
    • >TC000711 RA
      ccagttgct tcggctttc gaaatctca aacatctct 
      tcaccgata acaacaaat gatgtaaat tatgccata 
      taatgataa tacgtgtta tcaaggtca aaaaacccg 
      aactttttt caattcaaa taaaccaga tagtgcgtg 
      gtgtttgtg agtgttgtg agttcattc ggacgaaat 
      ggctttaaa taaaatttg tacgctgtg tttgcaaca 
      aagcagaaa cgaactcag agttttaaa tagtctgat 
      caataaaag acaccattt tgtttacac gccagacgt 
      aaaatcacc tgttaaagg tgaaaccca gaaaatgaa 
      catgttcca agcgatcaa caacgccct agacctggc 
      gttgaaaca ggacgaatc agccttaat tttcggcga 
      ggacgtcgc ctttggcgg tgtgttccg ctgcacgat 
      gggcttgca gtcaaagta cggtcctgg tcgcgtatt 
      caacacccc cctgtgtga acagggaat tgttggttt 
      tgcgatcgg tgcagccaa catgggttc aacagcaat 
      agcagaaat tcaattcgc cgattacac atttccggc 
      attcgatca gctggtgaa tgaagctgc gaaaatgag 
      gtacaggag tggggggca atatgattg cggaaaatt 
      aactgtcag ggcgccctg cggagccgt aggacatgg 
      agggcttta ccattcaca aagtcctga ggcgtattt 
      cgctcatac tccaggatt aaaggtcgt cataccacg 
      cgggccaat aaaagccaa aggcctgct ctcggcctg 
      catccgaga cccggatcc ttgcattat cttcgagcc 
      taagaccct atatagggc agctgtgga agaagtgcc 
      tgtcgacga ttacgttct cccgatagg aagagcaga 
      cgttttgct cgaaggcaa taatgtgac cttaatagg 
      atggggcac tcaggttca cgtgctgct cgaggtggc 
      tcaattggc caagaagca attgaacgt ttcctgtga 
      agtgatcga cttggtttc gattttgcc gtgggataa 
      aagcactgt ctgtcagtc tgtcaagaa aaccaagcg 
      agttttagt cgcccacga agcaccgct aaccggagg 
      attcggggc ggaattggc tgcaacaat tcaagagga 
      gtgtttcct gcatcttga ggcacctgt tgtgcgagt 
      cacgggctt cgacacgcc gttccctca cgttttcga 
      gcccttcta tctgccaga caagtggag atgtttgga 
      ggcgatacc cgcactcac ttcaagtgc taatctctg 
      attacgaac gattggaat taacaagtt gatttagtg 
      cttttgtat gtacgctcg tatatccca agcaaaata 
      gaggaaaaa cggaaactg aaaaaagtg caattattc 
      cagagaaat gtttccgct ttacactta ccgacgtta 
      ttatttctc attagtgaa taatgaata ataaaagct 
      ttgtgaatt t