| General information | |||
| TC000699 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 7358207..7362378 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC000699 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03062 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000699 RA
MGNHKKKRS QNGNLNRWI PDPMAVTWT EKYSDTINN FWKILPSTV RQSLATISI FLVGISING DWVNIFVHI ARQFGYTGR GKNSSGDVL FKWSEMSLE SLGLQDFWS YWMYSCLVS YLMYFVIGG FLHWYFYVR RRDRAEEWK CQPKKWLSP ELERHEIIL GSLTLFLNA STSALLACY IANGGYSTV YYNVADYGW LWFFLQWPV IFIYQDYVT YWMHRIYHT PFLYRNFHK LHHKYKQPT AFSVTAIHP VEALHIQLM DILPLFIIP THWFPFYVV ALYTYYHGI IDHSGVNFK AYWWQPWQP DAIFHDNHH QYFHVNFAF NIDYWDKLH GTYRRKDRV YTEDIFYGK GKRIDEVSA EELKADLKE RESENPLAY RDNKMEFKL SNRELKAMK RPKK
- >TC000699 RB
MGNHKKKRS QNGNLNRWI PDPMAVTWT EKYSDTINN FWKILPSTW YFYVRRRDR AEEWKCQPK KWLSPELER HEIILGSLT LFLNASTSA LLACYIANG GYSTVYYNV ADYGWLWFF LQWPVIFIY QDYVTYWMH RIYHTPFLY RNFHKLHHK YKQPTAFSV TAIHPVEAL HIQLMDILP LFIIPTHWF PFYVVALYT YYHGIIDHS GVNFKAYWW QPWQPDAIF HDNHHQYFH VNFAFNIDY WDKLHGTYR RKDRVYTED IFYGKGKRI DEVSAEELK ADLKERESE NPLAYRDNK MEFKLSNRE LKAMKRPKK
- >TC000699 RA
atgggtaat cacaaaaaa aaacgttct caaaacggg aatttaaat cgttggata ccagatcct atggctgta acatggact gaaaagtac agtgacact attaataat ttttggaaa atattgcca agcacggtc agacaatcg ttggcaacg atatctatt tttctagta ggaatctct attaatgga gattgggtg aatattttt gtgcatatt gcgcggcaa tttggatac actggtcgt ggaaagaac tcaagtggc gacgtcctt ttcaagtgg tccgaaatg tcattggag tctctagga ctacaagat ttctggagt tattggatg tattcatgt cttgtttca tatcttatg tattttgtt ataggtgga tttttacac tggtacttc tatgtacgc cgacgtgac cgagctgaa gaatggaaa tgccagccg aagaagtgg ttatctcct gaattggag agacacgaa attatcctc ggatctctg acacttttc ttgaatgcg tcgacttca gcgttgtta gcctgctac atcgccaac ggaggatac agcacggtc tattataac gttgcggac tacggctgg ttatggttt ttcctccaa tggcctgtc atttttatc taccaggat tatgtgacg tattggatg cataggatc taccataca ccgttcctt tatcgcaac ttccacaag ctccatcac aagtacaaa caacctacg gcgttttcc gtcaccgcc atccacccg gtggaagcc ctccatatt cagttgatg gacattctg ccccttttc atcattcct acgcactgg tttccgttc tacgtcgtg gccttgtac acatattac cacgggatc atcgatcac tctggtgtg aattttaaa gcatattgg tggcaaccg tggcaaccc gatgccatt tttcacgac aaccaccat caatatttc cacgtgaat ttcgctttt aatattgac tattgggat aagcttcac gggacttat cggcggaag gaccgggta tacaccgaa gacattttc tatgggaag ggcaaacgg attgacgaa gttagcgct gaagagctg aaagcggat ttgaaggaa agggagtcg gaaaacccg ttagcttac cgcgacaac aagatggag ttcaaattg tccaacaga gaactgaaa gcgatgaaa aggcccaaa aagtaa
- >TC000699 RB
atgggtaat cacaaaaaa aaacgttct caaaacggg aatttaaat cgttggata ccagatcct atggctgta acatggact gaaaagtac agtgacact attaataat ttttggaaa atattgcca agcacgtgg tacttctat gtacgccga cgtgaccga gctgaagaa tggaaatgc cagccgaag aagtggtta tctcctgaa ttggagaga cacgaaatt atcctcgga tctctgaca cttttcttg aatgcgtcg acttcagcg ttgttagcc tgctacatc gccaacgga ggatacagc acggtctat tataacgtt gcggactac ggctggtta tggtttttc ctccaatgg cctgtcatt tttatctac caggattat gtgacgtat tggatgcat aggatctac catacaccg ttcctttat cgcaacttc cacaagctc catcacaag tacaaacaa cctacggcg ttttccgtc accgccatc cacccggtg gaagccctc catattcag ttgatggac attctgccc cttttcatc attcctacg cactggttt ccgttctac gtcgtggcc ttgtacaca tattaccac gggatcatc gatcactct ggtgtgaat tttaaagca tattggtgg caaccgtgg caacccgat gccattttt cacgacaac caccatcaa tatttccac gtgaatttc gcttttaat attgactat tgggataag cttcacggg acttatcgg cggaaggac cgggtatac accgaagac attttctat gggaagggc aaacggatt gacgaagtt agcgctgaa gagctgaaa gcggatttg aaggaaagg gagtcggaa aacccgtta gcttaccgc gacaacaag atggagttc aaattgtcc aacagagaa ctgaaagcg atgaaaagg cccaaaaag taa
- >TC000699 RA
acgaaagtt tgaacaaac ttaaaattg gtgaattgc accaaagta aagatgggt aatcacaaa aaaaaacgt tctcaaaac gggaattta aatcgttgg ataccagat cctatggct gtaacatgg actgaaaag tacagtgac actattaat aatttttgg aaaatattg ccaagcacg gtcagacaa tcgttggca acgatatct atttttcta gtaggaatc tctattaat ggagattgg gtgaatatt tttgtgcat attgcgcgg caatttgga tacactggt cgtggaaag aactcaagt ggcgacgtc cttttcaag tggtccgaa atgtcattg gagtctcta ggactacaa gatttctgg agttattgg atgtattca tgtcttgtt tcatatctt atgtatttt gttataggt ggattttta cactggtac ttctatgta cgccgacgt gaccgagct gaagaatgg aaatgccag ccgaagaag tggttatct cctgaattg gagagacac gaaattatc ctcggatct ctgacactt ttcttgaat gcgtcgact tcagcgttg ttagcctgc tacatcgcc aacggagga tacagcacg gtctattat aacgttgcg gactacggc tggttatgg tttttcctc caatggcct gtcattttt atctaccag gattatgtg acgtattgg atgcatagg atctaccat acaccgttc ctttatcgc aacttccac aagctccat cacaagtac aaacaacct acggcgttt tccgtcacc gccatccac ccggtggaa gccctccat attcagttg atggacatt ctgcccctt ttcatcatt cctacgcac tggtttccg ttctacgtc gtggccttg tacacatat taccacggg atcatcgat cactctggt gtgaatttt aaagcatat tggtggcaa ccgtggcaa cccgatgcc atttttcac gacaaccac catcaatat ttccacgtg aatttcgct tttaatatt gactattgg gataagctt cacgggact tatcggcgg aaggaccgg gtatacacc gaagacatt ttctatggg aagggcaaa cggattgac gaagttagc gctgaagag ctgaaagcg gatttgaag gaaagggag tcggaaaac ccgttagct taccgcgac aacaagatg gagttcaaa ttgtccaac agagaactg aaagcgatg aaaaggccc aaaaagtaa ttcgatccc agaaacact aattctaaa tgagataat atacctaac atgtgatgt aataaagga acatttcac at
- >TC000699 RB
acgaaagtt tgaacaaac ttaaaattg gtgaattgc accaaagta aagatgggt aatcacaaa aaaaaacgt tctcaaaac gggaattta aatcgttgg ataccagat cctatggct gtaacatgg actgaaaag tacagtgac actattaat aatttttgg aaaatattg ccaagcacg tggtacttc tatgtacgc cgacgtgac cgagctgaa gaatggaaa tgccagccg aagaagtgg ttatctcct gaattggag agacacgaa attatcctc ggatctctg acacttttc ttgaatgcg tcgacttca gcgttgtta gcctgctac atcgccaac ggaggatac agcacggtc tattataac gttgcggac tacggctgg ttatggttt ttcctccaa tggcctgtc atttttatc taccaggat tatgtgacg tattggatg cataggatc taccataca ccgttcctt tatcgcaac ttccacaag ctccatcac aagtacaaa caacctacg gcgttttcc gtcaccgcc atccacccg gtggaagcc ctccatatt cagttgatg gacattctg ccccttttc atcattcct acgcactgg tttccgttc tacgtcgtg gccttgtac acatattac cacgggatc atcgatcac tctggtgtg aattttaaa gcatattgg tggcaaccg tggcaaccc gatgccatt tttcacgac aaccaccat caatatttc cacgtgaat ttcgctttt aatattgac tattgggat aagcttcac gggacttat cggcggaag gaccgggta tacaccgaa gacattttc tatgggaag ggcaaacgg attgacgaa gttagcgct gaagagctg aaagcggat ttgaaggaa agggagtcg gaaaacccg ttagcttac cgcgacaac aagatggag ttcaaattg tccaacaga gaactgaaa gcgatgaaa aggcccaaa aagtaattc gatcccaga aacactaat tctaaatga gataatata cctaacatg tgatgtaat aaaggaaca tttcacat
