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Gene information
General information
TC000699 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 1851664..1855835
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000699
    iBeetle screen iB_03062
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000699 RA
      MGNHKKKRS QNGNLNRWI PDPMAVTWT EKYSDTINN 
      FWKILPSTV RQSLATISI FLVGISING DWVNIFVHI 
      ARQFGYTGR GKNSSGDVL FKWSEMSLE SLGLQDFWS 
      YWMYSCLVS YLMYFVIGG FLHWYFYVR RRDRAEEWK 
      CQPKKWLSP ELERHEIIL GSLTLFLNA STSALLACY 
      IANGGYSTV YYNVADYGW LWFFLQWPV IFIYQDYVT 
      YWMHRIYHT PFLYRNFHK LHHKYKQPT AFSVTAIHP 
      VEALHIQLM DILPLFIIP THWFPFYVV ALYTYYHGI 
      IDHSGVNFK AYWWQPWQP DAIFHDNHH QYFHVNFAF 
      NIDYWDKLH GTYRRKDRV YTEDIFYGK GKRIDEVSA 
      EELKADLKE RESENPLAY RDNKMEFKL SNRELKAMK 
      RPKK
    • >TC000699 RB
      MGNHKKKRS QNGNLNRWI PDPMAVTWT EKYSDTINN 
      FWKILPSTW YFYVRRRDR AEEWKCQPK KWLSPELER 
      HEIILGSLT LFLNASTSA LLACYIANG GYSTVYYNV 
      ADYGWLWFF LQWPVIFIY QDYVTYWMH RIYHTPFLY 
      RNFHKLHHK YKQPTAFSV TAIHPVEAL HIQLMDILP 
      LFIIPTHWF PFYVVALYT YYHGIIDHS GVNFKAYWW 
      QPWQPDAIF HDNHHQYFH VNFAFNIDY WDKLHGTYR 
      RKDRVYTED IFYGKGKRI DEVSAEELK ADLKERESE 
      NPLAYRDNK MEFKLSNRE LKAMKRPKK
    CDS sequence
    • >TC000699 RA
      atgggtaat cacaaaaaa aaacgttct caaaacggg 
      aatttaaat cgttggata ccagatcct atggctgta 
      acatggact gaaaagtac agtgacact attaataat 
      ttttggaaa atattgcca agcacggtc agacaatcg 
      ttggcaacg atatctatt tttctagta ggaatctct 
      attaatgga gattgggtg aatattttt gtgcatatt 
      gcgcggcaa tttggatac actggtcgt ggaaagaac 
      tcaagtggc gacgtcctt ttcaagtgg tccgaaatg 
      tcattggag tctctagga ctacaagat ttctggagt 
      tattggatg tattcatgt cttgtttca tatcttatg 
      tattttgtt ataggtgga tttttacac tggtacttc 
      tatgtacgc cgacgtgac cgagctgaa gaatggaaa 
      tgccagccg aagaagtgg ttatctcct gaattggag 
      agacacgaa attatcctc ggatctctg acacttttc 
      ttgaatgcg tcgacttca gcgttgtta gcctgctac 
      atcgccaac ggaggatac agcacggtc tattataac 
      gttgcggac tacggctgg ttatggttt ttcctccaa 
      tggcctgtc atttttatc taccaggat tatgtgacg 
      tattggatg cataggatc taccataca ccgttcctt 
      tatcgcaac ttccacaag ctccatcac aagtacaaa 
      caacctacg gcgttttcc gtcaccgcc atccacccg 
      gtggaagcc ctccatatt cagttgatg gacattctg 
      ccccttttc atcattcct acgcactgg tttccgttc 
      tacgtcgtg gccttgtac acatattac cacgggatc 
      atcgatcac tctggtgtg aattttaaa gcatattgg 
      tggcaaccg tggcaaccc gatgccatt tttcacgac 
      aaccaccat caatatttc cacgtgaat ttcgctttt 
      aatattgac tattgggat aagcttcac gggacttat 
      cggcggaag gaccgggta tacaccgaa gacattttc 
      tatgggaag ggcaaacgg attgacgaa gttagcgct 
      gaagagctg aaagcggat ttgaaggaa agggagtcg 
      gaaaacccg ttagcttac cgcgacaac aagatggag 
      ttcaaattg tccaacaga gaactgaaa gcgatgaaa 
      aggcccaaa aagtaa
    • >TC000699 RB
      atgggtaat cacaaaaaa aaacgttct caaaacggg 
      aatttaaat cgttggata ccagatcct atggctgta 
      acatggact gaaaagtac agtgacact attaataat 
      ttttggaaa atattgcca agcacgtgg tacttctat 
      gtacgccga cgtgaccga gctgaagaa tggaaatgc 
      cagccgaag aagtggtta tctcctgaa ttggagaga 
      cacgaaatt atcctcgga tctctgaca cttttcttg 
      aatgcgtcg acttcagcg ttgttagcc tgctacatc 
      gccaacgga ggatacagc acggtctat tataacgtt 
      gcggactac ggctggtta tggtttttc ctccaatgg 
      cctgtcatt tttatctac caggattat gtgacgtat 
      tggatgcat aggatctac catacaccg ttcctttat 
      cgcaacttc cacaagctc catcacaag tacaaacaa 
      cctacggcg ttttccgtc accgccatc cacccggtg 
      gaagccctc catattcag ttgatggac attctgccc 
      cttttcatc attcctacg cactggttt ccgttctac 
      gtcgtggcc ttgtacaca tattaccac gggatcatc 
      gatcactct ggtgtgaat tttaaagca tattggtgg 
      caaccgtgg caacccgat gccattttt cacgacaac 
      caccatcaa tatttccac gtgaatttc gcttttaat 
      attgactat tgggataag cttcacggg acttatcgg 
      cggaaggac cgggtatac accgaagac attttctat 
      gggaagggc aaacggatt gacgaagtt agcgctgaa 
      gagctgaaa gcggatttg aaggaaagg gagtcggaa 
      aacccgtta gcttaccgc gacaacaag atggagttc 
      aaattgtcc aacagagaa ctgaaagcg atgaaaagg 
      cccaaaaag taa
    mRNA sequence
    • >TC000699 RA
      acgaaagtt tgaacaaac ttaaaattg gtgaattgc 
      accaaagta aagatgggt aatcacaaa aaaaaacgt 
      tctcaaaac gggaattta aatcgttgg ataccagat 
      cctatggct gtaacatgg actgaaaag tacagtgac 
      actattaat aatttttgg aaaatattg ccaagcacg 
      gtcagacaa tcgttggca acgatatct atttttcta 
      gtaggaatc tctattaat ggagattgg gtgaatatt 
      tttgtgcat attgcgcgg caatttgga tacactggt 
      cgtggaaag aactcaagt ggcgacgtc cttttcaag 
      tggtccgaa atgtcattg gagtctcta ggactacaa 
      gatttctgg agttattgg atgtattca tgtcttgtt 
      tcatatctt atgtatttt gttataggt ggattttta 
      cactggtac ttctatgta cgccgacgt gaccgagct 
      gaagaatgg aaatgccag ccgaagaag tggttatct 
      cctgaattg gagagacac gaaattatc ctcggatct 
      ctgacactt ttcttgaat gcgtcgact tcagcgttg 
      ttagcctgc tacatcgcc aacggagga tacagcacg 
      gtctattat aacgttgcg gactacggc tggttatgg 
      tttttcctc caatggcct gtcattttt atctaccag 
      gattatgtg acgtattgg atgcatagg atctaccat 
      acaccgttc ctttatcgc aacttccac aagctccat 
      cacaagtac aaacaacct acggcgttt tccgtcacc 
      gccatccac ccggtggaa gccctccat attcagttg 
      atggacatt ctgcccctt ttcatcatt cctacgcac 
      tggtttccg ttctacgtc gtggccttg tacacatat 
      taccacggg atcatcgat cactctggt gtgaatttt 
      aaagcatat tggtggcaa ccgtggcaa cccgatgcc 
      atttttcac gacaaccac catcaatat ttccacgtg 
      aatttcgct tttaatatt gactattgg gataagctt 
      cacgggact tatcggcgg aaggaccgg gtatacacc 
      gaagacatt ttctatggg aagggcaaa cggattgac 
      gaagttagc gctgaagag ctgaaagcg gatttgaag 
      gaaagggag tcggaaaac ccgttagct taccgcgac 
      aacaagatg gagttcaaa ttgtccaac agagaactg 
      aaagcgatg aaaaggccc aaaaagtaa ttcgatccc 
      agaaacact aattctaaa tgagataat atacctaac 
      atgtgatgt aataaagga acatttcac at
    • >TC000699 RB
      acgaaagtt tgaacaaac ttaaaattg gtgaattgc 
      accaaagta aagatgggt aatcacaaa aaaaaacgt 
      tctcaaaac gggaattta aatcgttgg ataccagat 
      cctatggct gtaacatgg actgaaaag tacagtgac 
      actattaat aatttttgg aaaatattg ccaagcacg 
      tggtacttc tatgtacgc cgacgtgac cgagctgaa 
      gaatggaaa tgccagccg aagaagtgg ttatctcct 
      gaattggag agacacgaa attatcctc ggatctctg 
      acacttttc ttgaatgcg tcgacttca gcgttgtta 
      gcctgctac atcgccaac ggaggatac agcacggtc 
      tattataac gttgcggac tacggctgg ttatggttt 
      ttcctccaa tggcctgtc atttttatc taccaggat 
      tatgtgacg tattggatg cataggatc taccataca 
      ccgttcctt tatcgcaac ttccacaag ctccatcac 
      aagtacaaa caacctacg gcgttttcc gtcaccgcc 
      atccacccg gtggaagcc ctccatatt cagttgatg 
      gacattctg ccccttttc atcattcct acgcactgg 
      tttccgttc tacgtcgtg gccttgtac acatattac 
      cacgggatc atcgatcac tctggtgtg aattttaaa 
      gcatattgg tggcaaccg tggcaaccc gatgccatt 
      tttcacgac aaccaccat caatatttc cacgtgaat 
      ttcgctttt aatattgac tattgggat aagcttcac 
      gggacttat cggcggaag gaccgggta tacaccgaa 
      gacattttc tatgggaag ggcaaacgg attgacgaa 
      gttagcgct gaagagctg aaagcggat ttgaaggaa 
      agggagtcg gaaaacccg ttagcttac cgcgacaac 
      aagatggag ttcaaattg tccaacaga gaactgaaa 
      gcgatgaaa aggcccaaa aagtaattc gatcccaga 
      aacactaat tctaaatga gataatata cctaacatg 
      tgatgtaat aaaggaaca tttcacat