Status
Please wait ...
Gene information
General information
TC000699 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7358207..7362378
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000699
    iBeetle screen iB_03062

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000699 RA
      MGNHKKKRS QNGNLNRWI PDPMAVTWT EKYSDTINN 
FWKILPSTV RQSLATISI FLVGISING DWVNIFVHI 
ARQFGYTGR GKNSSGDVL FKWSEMSLE SLGLQDFWS 
YWMYSCLVS YLMYFVIGG FLHWYFYVR RRDRAEEWK 
CQPKKWLSP ELERHEIIL GSLTLFLNA STSALLACY 
IANGGYSTV YYNVADYGW LWFFLQWPV IFIYQDYVT 
YWMHRIYHT PFLYRNFHK LHHKYKQPT AFSVTAIHP 
VEALHIQLM DILPLFIIP THWFPFYVV ALYTYYHGI 
IDHSGVNFK AYWWQPWQP DAIFHDNHH QYFHVNFAF 
NIDYWDKLH GTYRRKDRV YTEDIFYGK GKRIDEVSA 
EELKADLKE RESENPLAY RDNKMEFKL SNRELKAMK 
RPKK
    • >TC000699 RB
      MGNHKKKRS QNGNLNRWI PDPMAVTWT EKYSDTINN 
FWKILPSTW YFYVRRRDR AEEWKCQPK KWLSPELER 
HEIILGSLT LFLNASTSA LLACYIANG GYSTVYYNV 
ADYGWLWFF LQWPVIFIY QDYVTYWMH RIYHTPFLY 
RNFHKLHHK YKQPTAFSV TAIHPVEAL HIQLMDILP 
LFIIPTHWF PFYVVALYT YYHGIIDHS GVNFKAYWW 
QPWQPDAIF HDNHHQYFH VNFAFNIDY WDKLHGTYR 
RKDRVYTED IFYGKGKRI DEVSAEELK ADLKERESE 
NPLAYRDNK MEFKLSNRE LKAMKRPKK
    CDS sequence
    • >TC000699 RA
      atgggtaat cacaaaaaa aaacgttct caaaacggg 
aatttaaat cgttggata ccagatcct atggctgta 
acatggact gaaaagtac agtgacact attaataat 
ttttggaaa atattgcca agcacggtc agacaatcg 
ttggcaacg atatctatt tttctagta ggaatctct 
attaatgga gattgggtg aatattttt gtgcatatt 
gcgcggcaa tttggatac actggtcgt ggaaagaac 
tcaagtggc gacgtcctt ttcaagtgg tccgaaatg 
tcattggag tctctagga ctacaagat ttctggagt 
tattggatg tattcatgt cttgtttca tatcttatg 
tattttgtt ataggtgga tttttacac tggtacttc 
tatgtacgc cgacgtgac cgagctgaa gaatggaaa 
tgccagccg aagaagtgg ttatctcct gaattggag 
agacacgaa attatcctc ggatctctg acacttttc 
ttgaatgcg tcgacttca gcgttgtta gcctgctac 
atcgccaac ggaggatac agcacggtc tattataac 
gttgcggac tacggctgg ttatggttt ttcctccaa 
tggcctgtc atttttatc taccaggat tatgtgacg 
tattggatg cataggatc taccataca ccgttcctt 
tatcgcaac ttccacaag ctccatcac aagtacaaa 
caacctacg gcgttttcc gtcaccgcc atccacccg 
gtggaagcc ctccatatt cagttgatg gacattctg 
ccccttttc atcattcct acgcactgg tttccgttc 
tacgtcgtg gccttgtac acatattac cacgggatc 
atcgatcac tctggtgtg aattttaaa gcatattgg 
tggcaaccg tggcaaccc gatgccatt tttcacgac 
aaccaccat caatatttc cacgtgaat ttcgctttt 
aatattgac tattgggat aagcttcac gggacttat 
cggcggaag gaccgggta tacaccgaa gacattttc 
tatgggaag ggcaaacgg attgacgaa gttagcgct 
gaagagctg aaagcggat ttgaaggaa agggagtcg 
gaaaacccg ttagcttac cgcgacaac aagatggag 
ttcaaattg tccaacaga gaactgaaa gcgatgaaa 
aggcccaaa aagtaa
    • >TC000699 RB
      atgggtaat cacaaaaaa aaacgttct caaaacggg 
aatttaaat cgttggata ccagatcct atggctgta 
acatggact gaaaagtac agtgacact attaataat 
ttttggaaa atattgcca agcacgtgg tacttctat 
gtacgccga cgtgaccga gctgaagaa tggaaatgc 
cagccgaag aagtggtta tctcctgaa ttggagaga 
cacgaaatt atcctcgga tctctgaca cttttcttg 
aatgcgtcg acttcagcg ttgttagcc tgctacatc 
gccaacgga ggatacagc acggtctat tataacgtt 
gcggactac ggctggtta tggtttttc ctccaatgg 
cctgtcatt tttatctac caggattat gtgacgtat 
tggatgcat aggatctac catacaccg ttcctttat 
cgcaacttc cacaagctc catcacaag tacaaacaa 
cctacggcg ttttccgtc accgccatc cacccggtg 
gaagccctc catattcag ttgatggac attctgccc 
cttttcatc attcctacg cactggttt ccgttctac 
gtcgtggcc ttgtacaca tattaccac gggatcatc 
gatcactct ggtgtgaat tttaaagca tattggtgg 
caaccgtgg caacccgat gccattttt cacgacaac 
caccatcaa tatttccac gtgaatttc gcttttaat 
attgactat tgggataag cttcacggg acttatcgg 
cggaaggac cgggtatac accgaagac attttctat 
gggaagggc aaacggatt gacgaagtt agcgctgaa 
gagctgaaa gcggatttg aaggaaagg gagtcggaa 
aacccgtta gcttaccgc gacaacaag atggagttc 
aaattgtcc aacagagaa ctgaaagcg atgaaaagg 
cccaaaaag taa
    mRNA sequence
    • >TC000699 RA
      acgaaagtt tgaacaaac ttaaaattg gtgaattgc 
accaaagta aagatgggt aatcacaaa aaaaaacgt 
tctcaaaac gggaattta aatcgttgg ataccagat 
cctatggct gtaacatgg actgaaaag tacagtgac 
actattaat aatttttgg aaaatattg ccaagcacg 
gtcagacaa tcgttggca acgatatct atttttcta 
gtaggaatc tctattaat ggagattgg gtgaatatt 
tttgtgcat attgcgcgg caatttgga tacactggt 
cgtggaaag aactcaagt ggcgacgtc cttttcaag 
tggtccgaa atgtcattg gagtctcta ggactacaa 
gatttctgg agttattgg atgtattca tgtcttgtt 
tcatatctt atgtatttt gttataggt ggattttta 
cactggtac ttctatgta cgccgacgt gaccgagct 
gaagaatgg aaatgccag ccgaagaag tggttatct 
cctgaattg gagagacac gaaattatc ctcggatct 
ctgacactt ttcttgaat gcgtcgact tcagcgttg 
ttagcctgc tacatcgcc aacggagga tacagcacg 
gtctattat aacgttgcg gactacggc tggttatgg 
tttttcctc caatggcct gtcattttt atctaccag 
gattatgtg acgtattgg atgcatagg atctaccat 
acaccgttc ctttatcgc aacttccac aagctccat 
cacaagtac aaacaacct acggcgttt tccgtcacc 
gccatccac ccggtggaa gccctccat attcagttg 
atggacatt ctgcccctt ttcatcatt cctacgcac 
tggtttccg ttctacgtc gtggccttg tacacatat 
taccacggg atcatcgat cactctggt gtgaatttt 
aaagcatat tggtggcaa ccgtggcaa cccgatgcc 
atttttcac gacaaccac catcaatat ttccacgtg 
aatttcgct tttaatatt gactattgg gataagctt 
cacgggact tatcggcgg aaggaccgg gtatacacc 
gaagacatt ttctatggg aagggcaaa cggattgac 
gaagttagc gctgaagag ctgaaagcg gatttgaag 
gaaagggag tcggaaaac ccgttagct taccgcgac 
aacaagatg gagttcaaa ttgtccaac agagaactg 
aaagcgatg aaaaggccc aaaaagtaa ttcgatccc 
agaaacact aattctaaa tgagataat atacctaac 
atgtgatgt aataaagga acatttcac at
    • >TC000699 RB
      acgaaagtt tgaacaaac ttaaaattg gtgaattgc 
accaaagta aagatgggt aatcacaaa aaaaaacgt 
tctcaaaac gggaattta aatcgttgg ataccagat 
cctatggct gtaacatgg actgaaaag tacagtgac 
actattaat aatttttgg aaaatattg ccaagcacg 
tggtacttc tatgtacgc cgacgtgac cgagctgaa 
gaatggaaa tgccagccg aagaagtgg ttatctcct 
gaattggag agacacgaa attatcctc ggatctctg 
acacttttc ttgaatgcg tcgacttca gcgttgtta 
gcctgctac atcgccaac ggaggatac agcacggtc 
tattataac gttgcggac tacggctgg ttatggttt 
ttcctccaa tggcctgtc atttttatc taccaggat 
tatgtgacg tattggatg cataggatc taccataca 
ccgttcctt tatcgcaac ttccacaag ctccatcac 
aagtacaaa caacctacg gcgttttcc gtcaccgcc 
atccacccg gtggaagcc ctccatatt cagttgatg 
gacattctg ccccttttc atcattcct acgcactgg 
tttccgttc tacgtcgtg gccttgtac acatattac 
cacgggatc atcgatcac tctggtgtg aattttaaa 
gcatattgg tggcaaccg tggcaaccc gatgccatt 
tttcacgac aaccaccat caatatttc cacgtgaat 
ttcgctttt aatattgac tattgggat aagcttcac 
gggacttat cggcggaag gaccgggta tacaccgaa 
gacattttc tatgggaag ggcaaacgg attgacgaa 
gttagcgct gaagagctg aaagcggat ttgaaggaa 
agggagtcg gaaaacccg ttagcttac cgcgacaac 
aagatggag ttcaaattg tccaacaga gaactgaaa 
gcgatgaaa aggcccaaa aagtaattc gatcccaga 
aacactaat tctaaatga gataatata cctaacatg 
tgatgtaat aaaggaaca tttcacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY