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Gene information
General information
TC000676 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 2133201..2136186
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000676
    iBeetle screen iB_03060
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000676 RA
      MNFLIRSLC VLLRALDAL AAPIFWLKS RGKKRAVPT 
      IKDRLLKIS ATDLAEKIR TGELSSEQI CAAYVKRIK 
      EVNPLLNAV VEERFESAL QDARNVDIY LQSLPERAE 
      LAKTKPLLG VPLTVKESC SLAGLSLCG GTVSRAGIK 
      ADQDGEVVA KLKASGAIP LLVSNTPEI CLSWESSNF 
      VTGQTNNPY DVTRTSSGS SGGEGALLG AGASLIGIG 
      SDVAGSIRL PAMFNCVFG HKPTARTIP IKGHFPYCT 
      DERYADFFA IGPMTRYSK DLKLMMKVM ANEKLLPDL 
      RLEEKVDLG KIRVFFMEE ESKSFVSPR VQDEISQAI 
      RQSVEYLKV KCNCEIISD FKFAELKNS CEIAGSALY 
      SLDDIPNLL KAENQNLAL ELLKSVIGQ SNYTFSLLF 
      FYTLQYIFK TFVTNEGYL KKNEYLKQL FTEKLGQDG 
      VFLYPTFTT SAFHHDSFL FKSMGVSYL MIFNSLGLP 
      ATHVPCGLD KNGLPVGIQ VVAAPYQDR LCFAVAEEL 
      EKCFNGWIS PN
    • >TC000676 RB
      MNFLIRSLC VLLRALDAL AAPIFWLKS RGKKRAVPT 
      IKDRLLKIS ATDLAEKIR TGELSSEQI CAAYVKRIK 
      EVNPLLNAV VEERFESAL QDARNVDIY LQSLPERAE 
      LAKTKPLLG VPLTVKESC SLAADAIAV AIITRQFYS 
      SSNAKRDG
    • >TC000676 RC
      MCLSLCGGT VSRAGIKAD QDGEVVAKL KASGAIPLL 
      VSNTPEICL SWESSNFVT GQTNNPYDV TRTSSGSSG 
      GEGALLGAG ASLIGIGSD VAGSIRLPA MFNCVFGHK 
      PTARTIPIK GHFPYCTDE RYADFFAIG PMTRYSKDL 
      KLMMKVMAN EKLLPDLRL EEKVDLGKI RVFFMEEES 
      KSFVSPRVQ DEISQAIRQ SVEYLKVKC NCEIISDFK 
      FAELKNSCE IAGSALYSL DDIPNLLKA ENQNLALEL 
      LKSVIGQSN YTFSLLFFY TLQYIFKTF VTNEGYLKK 
      NEYLKQLFT EKLGQDGVF LYPTFTTSA FHHDSFLFK 
      SMGVSYLMI FNSLGLPAT HVPCGLDKN GLPVGIQVV 
      AAPYQDRLC FAVAEELEK CFNGWISPN
    CDS sequence
    • >TC000676 RA
      atgaatttt ctcatccgc tcgctgtgc gtgctcctg 
      cgggcgctg gacgcgctg gcggccccc atcttctgg 
      ctgaagtcc cgcgggaaa aagcgggca gtgcccacg 
      atcaaggac cgccttttg aaaatcagc gcgaccgac 
      ttggccgaa aaaatccgc accggagag ttgagcagc 
      gagcagatt tgcgcggct tatgtcaag cggatcaag 
      gaagtcaat cctttgctt aacgccgtc gtggaggag 
      cgcttcgaa agtgcattg caagatgca cgtaatgtc 
      gatatctat ctgcaatcg ctgccagaa cgcgcagag 
      ttagccaag acgaagcct ttattagga gtaccgttg 
      accgtaaag gaaagttgc tcacttgca ggcctcagc 
      ctttgcggg gggacggtg tccagggcc ggaatcaaa 
      gcagatcaa gacggggaa gtggttgcg aagttgaaa 
      gccagtggg gccatcccc ctccttgtc tccaacacc 
      ccagaaatt tgcctaagt tgggaaagc agcaatttc 
      gtgacgggt caaacaaac aacccttac gacgtcacc 
      cggactagc agcgggtca tcggggggc gaaggggcc 
      ctgctgggg gctggcgct tcgctgatt gggatcggg 
      tcagatgtt gccgggtcg attcggctg ccggcaatg 
      tttaactgt gtttttggg cacaagccg acagcaaga 
      acgattccg ataaaaggg cactttccg tactgcacg 
      gacgagcga tacgctgac ttttttgca ataggaccg 
      atgacccgg tactcgaag gatttaaaa ctgatgatg 
      aaagtgatg gcgaatgaa aaacttttg ccggatttg 
      aggctggag gaaaaagtt gatttgggg aaaattcgc 
      gtgtttttc atggaggag gaaagcaaa tcgtttgtg 
      tcaccgcga gtgcaagac gaaatcagt caagcgatt 
      agacaaagt gttgaatat ttgaaagtt aaatgtaat 
      tgtgaaata attagcgac tttaaattc gcggaatta 
      aagaatagt tgcgaaatt gcgggatcg gcgctttac 
      agcttggat gacattcct aatttatta aaagctgag 
      aaccaaaat ctggctctc gagctactc aagagtgtc 
      atcggacag tcaaattac acgtttagt ttacttttt 
      ttctacaca ttgcaatac attttcaaa acatttgtt 
      acaaatgag ggatatttg aaaaaaaac gaatattta 
      aaacagctt tttacagaa aaattgggt caagatgga 
      gtttttctt tacccaaca tttacaaca tcggcattt 
      caccacgat tcgttctta tttaagtca atgggagta 
      tcttattta atgattttt aattcgctc ggcttgcca 
      gcaacacat gtcccttgc ggacttgac aaaaatggg 
      ttgcctgtg ggaatacaa gttgtggca gccccttat 
      caggatcga ttatgtttt gctgtggcg gaagaatta 
      gaaaagtgt tttaatggg tggatatca ccaaattaa
    • >TC000676 RB
      atgaatttt ctcatccgc tcgctgtgc gtgctcctg 
      cgggcgctg gacgcgctg gcggccccc atcttctgg 
      ctgaagtcc cgcgggaaa aagcgggca gtgcccacg 
      atcaaggac cgccttttg aaaatcagc gcgaccgac 
      ttggccgaa aaaatccgc accggagag ttgagcagc 
      gagcagatt tgcgcggct tatgtcaag cggatcaag 
      gaagtcaat cctttgctt aacgccgtc gtggaggag 
      cgcttcgaa agtgcattg caagatgca cgtaatgtc 
      gatatctat ctgcaatcg ctgccagaa cgcgcagag 
      ttagccaag acgaagcct ttattagga gtaccgttg 
      accgtaaag gaaagttgc tcacttgca gctgatgca 
      attgcggtt gcaataatc actcgccag ttctattct 
      tcttcaaat gccaagcgt gacggatga
    • >TC000676 RC
      atgtgcctc agcctttgc ggggggacg gtgtccagg 
      gccggaatc aaagcagat caagacggg gaagtggtt 
      gcgaagttg aaagccagt ggggccatc cccctcctt 
      gtctccaac accccagaa atttgccta agttgggaa 
      agcagcaat ttcgtgacg ggtcaaaca aacaaccct 
      tacgacgtc acccggact agcagcggg tcatcgggg 
      ggcgaaggg gccctgctg ggggctggc gcttcgctg 
      attgggatc gggtcagat gttgccggg tcgattcgg 
      ctgccggca atgtttaac tgtgttttt gggcacaag 
      ccgacagca agaacgatt ccgataaaa gggcacttt 
      ccgtactgc acggacgag cgatacgct gactttttt 
      gcaatagga ccgatgacc cggtactcg aaggattta 
      aaactgatg atgaaagtg atggcgaat gaaaaactt 
      ttgccggat ttgaggctg gaggaaaaa gttgatttg 
      gggaaaatt cgcgtgttt ttcatggag gaggaaagc 
      aaatcgttt gtgtcaccg cgagtgcaa gacgaaatc 
      agtcaagcg attagacaa agtgttgaa tatttgaaa 
      gttaaatgt aattgtgaa ataattagc gactttaaa 
      ttcgcggaa ttaaagaat agttgcgaa attgcggga 
      tcggcgctt tacagcttg gatgacatt cctaattta 
      ttaaaagct gagaaccaa aatctggct ctcgagcta 
      ctcaagagt gtcatcgga cagtcaaat tacacgttt 
      agtttactt tttttctac acattgcaa tacattttc 
      aaaacattt gttacaaat gagggatat ttgaaaaaa 
      aacgaatat ttaaaacag ctttttaca gaaaaattg 
      ggtcaagat ggagttttt ctttaccca acatttaca 
      acatcggca tttcaccac gattcgttc ttatttaag 
      tcaatggga gtatcttat ttaatgatt tttaattcg 
      ctcggcttg ccagcaaca catgtccct tgcggactt 
      gacaaaaat gggttgcct gtgggaata caagttgtg 
      gcagcccct tatcaggat cgattatgt tttgctgtg 
      gcggaagaa ttagaaaag tgttttaat gggtggata 
      tcaccaaat taa
    mRNA sequence
    • >TC000676 RA
      gcacgcaac tggataaaa tgataagct cgctctaga 
      gctagtgtt ttctactca gtcccggcg aagagactt 
      tctttcagc ggagagagc agaaccgta aaatcacag 
      ttactttta gcaaaataa gacaagtag ttaaaatga 
      aagtgaaac taataaaca agtgtctta aacttaacg 
      aaaagtaaa cgtaatttg agaactagc attaaattc 
      attaacttg gccaaaacg ccgagtcaa ggtaacccc 
      gcacggtca atgcaaagc cattgatct tgaaagcac 
      ctgagcacc gaatttcag atgcgatga attttctca 
      tccgctcgc tgtgcgtgc tcctgcggg cgctggacg 
      cgctggcgg cccccatct tctggctga agtcccgcg 
      ggaaaaagc gggcagtgc ccacgatca aggaccgcc 
      ttttgaaaa tcagcgcga ccgacttgg ccgaaaaaa 
      tccgcaccg gagagttga gcagcgagc agatttgcg 
      cggcttatg tcaagcgga tcaaggaag tcaatcctt 
      tgcttaacg ccgtcgtgg aggagcgct tcgaaagtg 
      cattgcaag atgcacgta atgtcgata tctatctgc 
      aatcgctgc cagaacgcg cagagttag ccaagacga 
      agcctttat taggagtac cgttgaccg taaaggaaa 
      gttgctcac ttgcaggcc tcagccttt gcgggggga 
      cggtgtcca gggccggaa tcaaagcag atcaagacg 
      gggaagtgg ttgcgaagt tgaaagcca gtggggcca 
      tccccctcc ttgtctcca acaccccag aaatttgcc 
      taagttggg aaagcagca atttcgtga cgggtcaaa 
      caaacaacc cttacgacg tcacccgga ctagcagcg 
      ggtcatcgg ggggcgaag gggccctgc tgggggctg 
      gcgcttcgc tgattggga tcgggtcag atgttgccg 
      ggtcgattc ggctgccgg caatgttta actgtgttt 
      ttgggcaca agccgacag caagaacga ttccgataa 
      aagggcact ttccgtact gcacggacg agcgatacg 
      ctgactttt ttgcaatag gaccgatga cccggtact 
      cgaaggatt taaaactga tgatgaaag tgatggcga 
      atgaaaaac ttttgccgg atttgaggc tggaggaaa 
      aagttgatt tggggaaaa ttcgcgtgt ttttcatgg 
      aggaggaaa gcaaatcgt ttgtgtcac cgcgagtgc 
      aagacgaaa tcagtcaag cgattagac aaagtgttg 
      aatatttga aagttaaat gtaattgtg aaataatta 
      gcgacttta aattcgcgg aattaaaga atagttgcg 
      aaattgcgg gatcggcgc tttacagct tggatgaca 
      ttcctaatt tattaaaag ctgagaacc aaaatctgg 
      ctctcgagc tactcaaga gtgtcatcg gacagtcaa 
      attacacgt ttagtttac tttttttct acacattgc 
      aatacattt tcaaaacat ttgttacaa atgagggat 
      atttgaaaa aaaacgaat atttaaaac agcttttta 
      cagaaaaat tgggtcaag atggagttt ttctttacc 
      caacattta caacatcgg catttcacc acgattcgt 
      tcttattta agtcaatgg gagtatctt atttaatga 
      tttttaatt cgctcggct tgccagcaa cacatgtcc 
      cttgcggac ttgacaaaa atgggttgc ctgtgggaa 
      tacaagttg tggcagccc cttatcagg atcgattat 
      gttttgctg tggcggaag aattagaaa agtgtttta 
      atgggtgga tatcaccaa attaatttt gtattacaa 
      taaaaaccg aaaaataaa atattgatt tttaa
    • >TC000676 RB
      gcacgcaac tggataaaa tgataagct cgctctaga 
      gctagtgtt ttctactca gtcccggcg aagagactt 
      tctttcagc ggagagagc agaaccgta aaatcacag 
      ttactttta gcaaaataa gacaagtag ttaaaatga 
      aagtgaaac taataaaca agtgtctta aacttaacg 
      aaaagtaaa cgtaatttg agaactagc attaaattc 
      attaacttg gccaaaacg ccgagtcaa ggtaacccc 
      gcacggtca atgcaaagc cattgatct tgaaagcac 
      ctgagcacc gaatttcag atgcgatga attttctca 
      tccgctcgc tgtgcgtgc tcctgcggg cgctggacg 
      cgctggcgg cccccatct tctggctga agtcccgcg 
      ggaaaaagc gggcagtgc ccacgatca aggaccgcc 
      ttttgaaaa tcagcgcga ccgacttgg ccgaaaaaa 
      tccgcaccg gagagttga gcagcgagc agatttgcg 
      cggcttatg tcaagcgga tcaaggaag tcaatcctt 
      tgcttaacg ccgtcgtgg aggagcgct tcgaaagtg 
      cattgcaag atgcacgta atgtcgata tctatctgc 
      aatcgctgc cagaacgcg cagagttag ccaagacga 
      agcctttat taggagtac cgttgaccg taaaggaaa 
      gttgctcac ttgcagctg atgcaattg cggttgcaa 
      taatcactc gccagttct attcttctt caaatgcca 
      agcgtgacg gatgaaaac cttttatca cccggtgac 
      taattgcaa ttaatccgt aattatttc agatttttt 
      gaaacttga ttgggtttt tatttccac aaggaaacg 
      ctttgcaaa ttacaccac atttttaat tacgtgtga 
      ggaggaagt cgcgcatat ttatttagc aaaaatgtc 
      aaacgcacg cgatgtttt gttaaagaa atagcgaaa 
      gtgcaatat acttgtgaa attactact gtttcgctt 
      ttccttatt agcgcttcc cattattac gtttcaatt 
      tttacgaac gttattatt ttggatgga aactgacgt 
      aaatatctc ggaaaaaac tcgtttaac tcaaacccg 
      tttccacca aaatacttt aattaattt gcattactg 
      agacaattt ccagaaatt aaccaaata aaaaaatgg 
      aaaggtt
    • >TC000676 RC
      ctattttaa taaattaaa gcgacattt gatgttgtg 
      cgtaatgaa cttgcgagt aattatctt ggaaaaaac 
      taattagca tgtgcctca gcctttgcg gggggacgg 
      tgtccaggg ccggaatca aagcagatc aagacgggg 
      aagtggttg cgaagttga aagccagtg gggccatcc 
      ccctccttg tctccaaca ccccagaaa tttgcctaa 
      gttgggaaa gcagcaatt tcgtgacgg gtcaaacaa 
      acaaccctt acgacgtca cccggacta gcagcgggt 
      catcggggg gcgaagggg ccctgctgg gggctggcg 
      cttcgctga ttgggatcg ggtcagatg ttgccgggt 
      cgattcggc tgccggcaa tgtttaact gtgtttttg 
      ggcacaagc cgacagcaa gaacgattc cgataaaag 
      ggcactttc cgtactgca cggacgagc gatacgctg 
      acttttttg caataggac cgatgaccc ggtactcga 
      aggatttaa aactgatga tgaaagtga tggcgaatg 
      aaaaacttt tgccggatt tgaggctgg aggaaaaag 
      ttgatttgg ggaaaattc gcgtgtttt tcatggagg 
      aggaaagca aatcgtttg tgtcaccgc gagtgcaag 
      acgaaatca gtcaagcga ttagacaaa gtgttgaat 
      atttgaaag ttaaatgta attgtgaaa taattagcg 
      actttaaat tcgcggaat taaagaata gttgcgaaa 
      ttgcgggat cggcgcttt acagcttgg atgacattc 
      ctaatttat taaaagctg agaaccaaa atctggctc 
      tcgagctac tcaagagtg tcatcggac agtcaaatt 
      acacgttta gtttacttt ttttctaca cattgcaat 
      acattttca aaacatttg ttacaaatg agggatatt 
      tgaaaaaaa acgaatatt taaaacagc tttttacag 
      aaaaattgg gtcaagatg gagtttttc tttacccaa 
      catttacaa catcggcat ttcaccacg attcgttct 
      tatttaagt caatgggag tatcttatt taatgattt 
      ttaattcgc tcggcttgc cagcaacac atgtccctt 
      gcggacttg acaaaaatg ggttgcctg tgggaatac 
      aagttgtgg cagcccctt atcaggatc gattatgtt 
      ttgctgtgg cggaagaat tagaaaagt gttttaatg 
      ggtggatat caccaaatt aattttgta ttacaataa 
      aaaccgaaa aataaaata ttgattttt aa