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Gene information
General information
TC000669 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7764016..7780063
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000669
    iBeetle screen iB_03058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen musculature partially irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000669 RA
      MAVIVILAL FLTVASRQS FVDTAPLNE GTPGDSPSS 
      QDTSVRELE SLKHSIGKT LEMIKSQSA VYTNNNGQK 
      QAHAERKEQ VNENGKLVS KIDQHIDME GKDSARPQG 
      KIQTEIDVP AKGIHKSVV QQLPEYQTL TENGNRRSE 
      SLSEPGNGI DKFNAFSGI QYSPLDMAE YVFWTGDEK 
      GVTLAIEEF LQEGLMTRE EAISFLQEI KYNLDYLKN 
      HYTQLGLNA KERSRTQDT QLKYSDKPE LKSSFDLNL 
      LRQEATKKR EPEVPHSIP MMKSNEGDK SQDEDYDEL 
      LERLRVADF LYTEYSLEE VIYQLAKVM FTQSLTRGS 
      AEAQQALQK FTNFLEIEA EQGHISRTL EKKVLDVLI 
      ASLSDTLTE HPELLSAAR ESLGATATN ADGQHFLRQ 
      ILEISPDDK AAVRAYKGQ AERNIPVQK ITNHVPLGL 
      GKYKNP
    CDS sequence
    • >TC000669 RA
      atggctgtg attgtaatt ctggctctg tttttaacg 
      gtggcatcc cgacaaagt ttcgtagat acggctcct 
      ttgaacgaa ggaacgccg ggagattcc ccttcatcg 
      caggatacg agcgtgcga gagcttgaa agtcttaaa 
      cattcaata ggcaaaact ttggaaatg ataaaaagc 
      cagtcggcg gtgtacacg aataacaac gggcaaaaa 
      caagcccac gctgagagg aaagagcaa gtaaatgaa 
      aatgggaag ttagtatcg aaaattgat cagcacatc 
      gacatggaa gggaaggac tcggcgagg cctcaaggt 
      aaaatacag actgagatt gacgtgccg gcgaaagga 
      attcacaaa agtgtcgtc caacaacta ccagaatat 
      caaacgttg acagaaaat ggaaacaga cgctctgag 
      agcctttca gagcctggg aatggcata gacaagttt 
      aacgccttc tcaggcata caatactcc cccctcgat 
      atggccgag tatgtgttc tggacaggc gacgagaaa 
      ggcgttact ctcgccatc gaagaattc ctacaagag 
      ggtttgatg actcgagaa gaagccatt tcgttcctc 
      caagaaatc aaatataac ttggactat ctcaagaac 
      cattacact caacttggc ctaaatgcc aaggaacgc 
      tccagaact caggacacg caattaaaa tattccgac 
      aaaccggaa ttgaagagc tcgttcgac ttaaaccta 
      ctgagacag gaagccacg aaaaaaaga gaaccagaa 
      gtaccacac tcgattccc atgatgaaa tcaaacgaa 
      ggtgacaag agtcaagac gaagactac gacgagctc 
      ctcgagcgt ctccgagtc gccgacttc ctatacacc 
      gagtactca ttagaagag gtcatctat cagctcgct 
      aaagtcatg tttacacag tctctcacg cggggaagt 
      gccgaggct caacaggca ctccagaaa ttcaccaac 
      tttcttgaa atcgaggcc gaacaaggg cacatttcc 
      aggaccctc gaaaagaaa gttctagac gtgctgatt 
      gcttctttg tcagacaca ctcacggaa catccggaa 
      cttctctcg gcggctaga gaaagttta ggagcgacg 
      gccactaat gcagatgga cagcatttc ctgagacaa 
      atcctcgaa attagtccg gacgataaa gctgcagtt 
      agagcttac aaaggacaa gcggaacgg aatattccg 
      gtgcagaaa atcaccaac catgttccc ctagggctc 
      ggcaaatac aaaaaccca taa
    mRNA sequence
    • >TC000669 RA
      gcagttgtc cgcgatcct gcaggattc gtacgttta 
      ggcgcgtgc tttgcaaca gctgtgaca ctttggact 
      gcggcatgg ctgtgattg taattctgg ctctgtttt 
      taacggtgg catcccgac aaagtttcg tagatacgg 
      ctcctttga acgaaggaa cgccgggag attcccctt 
      catcgcagg atacgagcg tgcgagagc ttgaaagtc 
      ttaaacatt caataggca aaactttgg aaatgataa 
      aaagccagt cggcggtgt acacgaata acaacgggc 
      aaaaacaag cccacgctg agaggaaag agcaagtaa 
      atgaaaatg ggaagttag tatcgaaaa ttgatcagc 
      acatcgaca tggaaggga aggactcgg cgaggcctc 
      aaggtaaaa tacagactg agattgacg tgccggcga 
      aaggaattc acaaaagtg tcgtccaac aactaccag 
      aatatcaaa cgttgacag aaaatggaa acagacgct 
      ctgagagcc tttcagagc ctgggaatg gcatagaca 
      agtttaacg ccttctcag gcatacaat actcccccc 
      tcgatatgg ccgagtatg tgttctgga caggcgacg 
      agaaaggcg ttactctcg ccatcgaag aattcctac 
      aagagggtt tgatgactc gagaagaag ccatttcgt 
      tcctccaag aaatcaaat ataacttgg actatctca 
      agaaccatt acactcaac ttggcctaa atgccaagg 
      aacgctcca gaactcagg acacgcaat taaaatatt 
      ccgacaaac cggaattga agagctcgt tcgacttaa 
      acctactga gacaggaag ccacgaaaa aaagagaac 
      cagaagtac cacactcga ttcccatga tgaaatcaa 
      acgaaggtg acaagagtc aagacgaag actacgacg 
      agctcctcg agcgtctcc gagtcgccg acttcctat 
      acaccgagt actcattag aagaggtca tctatcagc 
      tcgctaaag tcatgttta cacagtctc tcacgcggg 
      gaagtgccg aggctcaac aggcactcc agaaattca 
      ccaactttc ttgaaatcg aggccgaac aagggcaca 
      tttccagga ccctcgaaa agaaagttc tagacgtgc 
      tgattgctt ctttgtcag acacactca cggaacatc 
      cggaacttc tctcggcgg ctagagaaa gtttaggag 
      cgacggcca ctaatgcag atggacagc atttcctga 
      gacaaatcc tcgaaatta gtccggacg ataaagctg 
      cagttagag cttacaaag gacaagcgg aacggaata 
      ttccggtgc agaaaatca ccaaccatg ttcccctag 
      ggctcggca aatacaaaa acccataaa tatatctat 
      gataatcta cttaataaa gtgtaccta tatatgttt 
      caatgtaag ccgtttttg tctatgtcg tatggccca 
      aactaaata caatcaaat tttttgtaa gtcttgctt 
      agaatcaaa cgagacagg gttggtgca aatgtttta 
      ttttacccg attttcgcc atatggcat aaacaaaag 
      ccgattcaa ttcaaatat tttaataag ctcatacca 
      atacagtac aactaatgg aatttgtat tgagattgt 
      ggcgtcact gcaatttta attgatagt tcctatcga 
      cgtaccttt aatagtcat ttaatttcc acaaaaata 
      aatcttgta aatgtgttt aaaattaaa attgcagtc 
      gtttcgacg aaacaattt tctttcgct gcttttgtt 
      ctcgctcac cacttcaat cgtatgcaa ccaacagtt 
      ccgattctt taaaatcgt cttctgtga aagaaaagt 
      ctgggatat agtgactat aaactagta ggtaaaacg 
      gaatgaaag tcgagcatg tgaacatca taatcaaat 
      tttttcaca aaaagaact ggaccaaca aacactgta 
      ataactttg ttttctttt ggctctttt agggtgaaa 
      ggtagtagg agggatgga tgtagtggc tggctacat 
      aatgttaga ttgtacata gaattactg taagaacaa 
      aaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY