Status
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Gene information
General information
TC032118
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000631,TC000632
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3326383..3344000
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032118
    iBeetle screen iB_03055
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032118 RA
      MADDCADPE LFSPRRGSR QRNLSLKAK EYVSTSSRR 
      KRGFKKLNI STATESEDD SNCESSEKP TVLHDNDHV 
      PGDKLFTFQ TRKKCEVVQ QTPHAFRDK MRKNIVAKI 
      LEENGSSGD DFSASESEF EVSEENSEE SSSSDDTPP 
      PPQNKNIKP TLGARKVNY TIKTDDYFA QHASKKTLT 
      SNHTLDKLE TPRLPQDQL HKLLSRVSL GCEHEKATK 
      KLIETNCAY FDKWMYLMH ENFNILLYG LGSKKGVLN 
      AFHEKCLKK LPTVVVNGF FPSLSIKDV LDGIILNLL 
      ELKENPSNI YEACDLIER EFSFIPETH LYLIVHNID 
      MMRSGKAQS VFARLASVN NIHLIASID HINAPLIWD 
      HSKISKFNF TWWDVTTFQ RYIDETSFE SSMLVQRTG 
      NLALSSLRN VFLSLTSNS KGIYLILVK YQIENGKKQ 
      YYQGLAFKD LYSLCREAF LVSSDLALR AQLTEFVDH 
      KMVKFKRAP DGTDDPKGG LTHVKMALA RLPVVSDCS 
      AGQTARVSS NLHPTSTMA VSRVPSPPP QEVNTPVAE 
      NWCYTQVKV VKFSYMWTI NNFSFCREE MGEVLKSST 
      FSAGANDKL KWCLRVNPK GLDEESKDY LSLYLLLVS 
      CNKSEVRAK FKFSILNAK REETKAMES QRAYRFVQG 
      KDWGFKKFI RRDFLLDEA NGLLPDDKL TIFCEVSVV 
      ADSVNISGQ SNTIQFKVP ECRLSDDLG LLFENQKFS 
      DVTLSVGGR EFQAHKAIL AARSPVFQA MFEHEMEER 
      KHNRVDITD VDHEVLREM LRFIYTGKA SNLEKMADD 
      LLAAADKYA LERLKVMCE EALCTNLSI DNAAEILIL 
      ADLHSADQL KAQAIDFIN THATDVMDT PGWKSMIQT 
      HPHLIAEAF RALATQQIP PIGPPRKRV KQS
    CDS sequence
    • >TC032118 RA
      atggcagac gactgtgca gaccctgag ttattttcg 
      ccccgcagg ggttcccgg caacgcaat ttgagtcta 
      aaagctaaa gaatacgta tctacttcc agtaggagg 
      aaacgtggc tttaaaaaa ctgaatatt tctacagca 
      actgagtct gaggacgat tccaattgt gagagtagt 
      gaaaaacca acagttttg cacgataat gaccatgtc 
      cctggtgat aaattattc acgtttcaa acgcgcaaa 
      aagtgcgaa gtcgttcaa caaacgccg cacgctttt 
      agggacaaa atgaggaaa aatatcgtg gcgaaaatt 
      ttggaagag aatggttcg agcggagat gatttttca 
      gccagtgag agtgaattc gaagtgtct gaggaaaat 
      tcagaggag tcttccagc agtgacgac acaccgcct 
      cccccgcaa aacaaaaat attaaaccc actttagga 
      gcgagaaaa gtcaattac actataaaa acagacgat 
      tatttcgca caacacgcc agtaaaaaa actctaacg 
      tccaatcac actttagat aagttggag acaccaaga 
      ctgccgcaa gaccaactg cataaactt ttgtcgaga 
      gttagccta gggtgtgag catgaaaaa gccacgaaa 
      aaattaatc gagacgaat tgcgcctat tttgataag 
      tggatgtat ttgatgcat gaaaatttc aatattttg 
      ttgtatggg ttgggctca aagaagggc gttttgaat 
      gcttttcac gaaaagtgt ttgaagaag ttgccaacg 
      gttgtcgtg aacggattt ttcccttca ttatcaatt 
      aaagatgtc cttgacggg attatttta aatttacta 
      gaactgaag gaaaatccc tcaaatatt tacgaggcc 
      tgcgacctt atcgagcgc gaatttagt tttattccc 
      gaaactcat ttatacctc attgtgcac aacatcgac 
      atgatgcgg agtggaaaa gcccaaagt gtctttgca 
      agactcgcg tcagtcaat aacattcat ctaatcgcg 
      tcaattgac cacataaat gccccccta atttgggac 
      cattccaaa ataagtaaa ttcaatttc acgtggtgg 
      gacgttacc actttccaa cgctatatt gacgaaacg 
      tccttcgaa agctcaatg ttggtccaa aggaccgga 
      aacctggcc ttatcatcg cttcggaac gttttctta 
      tcactgacg agcaactcc aaaggaatt tacttaatc 
      ctcgtcaag tatcaaatc gaaaatggc aaaaaacaa 
      tactatcaa ggcctggcg tttaaagac ttgtactct 
      ttgtgccgc gaggcattt ttggtgagt tcggacctg 
      gcattgcgg gcccaactc accgaattc gtggaccac 
      aaaatggtg aagttcaag agagcgcca gacgggact 
      gatgaccca aaaggtgga ttaacccat gtcaagatg 
      gccttagct aggctgccg gtggtgagt gattgctcg 
      gccggccaa acggcccga gtcagctcc aacctgcat 
      cccacgagt accatggcc gttagccgc gtgccgagt 
      ccaccaccc caggaggta aacacccca gtggccgag 
      aactggtgt tacacgcag gtgaaagtc gtaaagttc 
      agctacatg tggacaata aataatttt agtttttgt 
      cgcgaagaa atgggcgaa gttttaaag tcttcgacg 
      ttttcggcg ggagctaac gataaatta aaatggtgt 
      ctaagggtg aaccctaaa ggtttggac gaggaaagc 
      aaagactat ctctcactg tacctcctt ctcgtctcg 
      tgcaataaa agcgaagtt cgagccaaa tttaaattt 
      agtatatta aatgcgaag agagaagaa acgaaggcg 
      atggagagt caaagggcg tacagattc gtgcagggg 
      aaggattgg gggttcaag aagttcatc aggagggac 
      tttctgttg gacgaagcc aacggtctt ttgcccgac 
      gataagctt acgatattt tgcgaagtc agtgttgtc 
      gctgatagt gtaaatatt tctggccag tcgaataca 
      attcaattt aaagtgcca gaatgtagg ttatcggac 
      gatttaggt ttacttttc gaaaatcaa aaatttagc 
      gatgttact ctttcggta ggcggcagg gagttccaa 
      gcacataag gctatatta gcagcgagg agtcccgta 
      ttccaggcc atgttcgaa catgaaatg gaagagagg 
      aaacataat agagtagat ataacggac gtagaccat 
      gaagtatta cgagaaatg cttagattt atctacaca 
      ggaaaagcg tcgaatttg gagaaaatg gcggacgat 
      ttgttggcc gccgccgat aagtatgcg ttagaacgg 
      ctcaaagtg atgtgtgag gaggcgttg tgtaccaac 
      ctatcaata gacaacgct gccgagatt ttaatattg 
      gcagacctg cacagcgcc gaccagttg aaagcacaa 
      gcaatagat tttataaat acgcacgcg acggacgtg 
      atggacaca cccggttgg aagagcatg atccaaacg 
      catcctcat ctgatcgcc gaggcgttc cgtgcgctg 
      gcgacccag cagatcccc cccataggt ccaccacga 
      aaaagggtg aaacagagc tga
    mRNA sequence
    • >TC032118 RA
      ttggcgggg ttacttcca aaaaacgtt tccatgagg 
      ttactagtg gtctgaaat ggcagacga ctgtgcaga 
      ccctgagtt attttcgcc ccgcagggg ttcccggca 
      acgcaattt gagtctaaa agctaaaga atacgtatc 
      tacttccag taggaggaa acgtggctt taaaaaact 
      gaatatttc tacagcaac tgagtctga ggacgattc 
      caattgtga gagtagtga aaaaccaac agttttgca 
      cgataatga ccatgtccc tggtgataa attattcac 
      gtttcaaac gcgcaaaaa gtgcgaagt cgttcaaca 
      aacgccgca cgcttttag ggacaaaat gaggaaaaa 
      tatcgtggc gaaaatttt ggaagagaa tggttcgag 
      cggagatga tttttcagc cagtgagag tgaattcga 
      agtgtctga ggaaaattc agaggagtc ttccagcag 
      tgacgacac accgcctcc cccgcaaaa caaaaatat 
      taaacccac tttaggagc gagaaaagt caattacac 
      tataaaaac agacgatta tttcgcaca acacgccag 
      taaaaaaac tctaacgtc caatcacac tttagataa 
      gttggagac accaagact gccgcaaga ccaactgca 
      taaactttt gtcgagagt tagcctagg gtgtgagca 
      tgaaaaagc cacgaaaaa attaatcga gacgaattg 
      cgcctattt tgataagtg gatgtattt gatgcatga 
      aaatttcaa tattttgtt gtatgggtt gggctcaaa 
      gaagggcgt tttgaatgc ttttcacga aaagtgttt 
      gaagaagtt gccaacggt tgtcgtgaa cggattttt 
      cccttcatt atcaattaa agatgtcct tgacgggat 
      tattttaaa tttactaga actgaagga aaatccctc 
      aaatattta cgaggcctg cgaccttat cgagcgcga 
      atttagttt tattcccga aactcattt atacctcat 
      tgtgcacaa catcgacat gatgcggag tggaaaagc 
      ccaaagtgt ctttgcaag actcgcgtc agtcaataa 
      cattcatct aatcgcgtc aattgacca cataaatgc 
      ccccctaat ttgggacca ttccaaaat aagtaaatt 
      caatttcac gtggtggga cgttaccac tttccaacg 
      ctatattga cgaaacgtc cttcgaaag ctcaatgtt 
      ggtccaaag gaccggaaa cctggcctt atcatcgct 
      tcggaacgt tttcttatc actgacgag caactccaa 
      aggaattta cttaatcct cgtcaagta tcaaatcga 
      aaatggcaa aaaacaata ctatcaagg cctggcgtt 
      taaagactt gtactcttt gtgccgcga ggcattttt 
      ggtgagttc ggacctggc attgcgggc ccaactcac 
      cgaattcgt ggaccacaa aatggtgaa gttcaagag 
      agcgccaga cgggactga tgacccaaa aggtggatt 
      aacccatgt caagatggc cttagctag gctgccggt 
      ggtgagtga ttgctcggc cggccaaac ggcccgagt 
      cagctccaa cctgcatcc cacgagtac catggccgt 
      tagccgcgt gccgagtcc accacccca ggaggtaaa 
      caccccagt ggccgagaa ctggtgtta cacgcaggt 
      gaaagtcgt aaagttcag ctacatgtg gacaataaa 
      taattttag tttttgtcg cgaagaaat gggcgaagt 
      tttaaagtc ttcgacgtt ttcggcggg agctaacga 
      taaattaaa atggtgtct aagggtgaa ccctaaagg 
      tttggacga ggaaagcaa agactatct ctcactgta 
      cctccttct cgtctcgtg caataaaag cgaagttcg 
      agccaaatt taaatttag tatattaaa tgcgaagag 
      agaagaaac gaaggcgat ggagagtca aagggcgta 
      cagattcgt gcaggggaa ggattgggg gttcaagaa 
      gttcatcag gagggactt tctgttgga cgaagccaa 
      cggtctttt gcccgacga taagcttac gatattttg 
      cgaagtcag tgttgtcgc tgatagtgt aaatatttc 
      tggccagtc gaatacaat tcaatttaa agtgccaga 
      atgtaggtt atcggacga tttaggttt acttttcga 
      aaatcaaaa atttagcga tgttactct ttcggtagg 
      cggcaggga gttccaagc acataaggc tatattagc 
      agcgaggag tcccgtatt ccaggccat gttcgaaca 
      tgaaatgga agagaggaa acataatag agtagatat 
      aacggacgt agaccatga agtattacg agaaatgct 
      tagatttat ctacacagg aaaagcgtc gaatttgga 
      gaaaatggc ggacgattt gttggccgc cgccgataa 
      gtatgcgtt agaacggct caaagtgat gtgtgagga 
      ggcgttgtg taccaacct atcaataga caacgctgc 
      cgagatttt aatattggc agacctgca cagcgccga 
      ccagttgaa agcacaagc aatagattt tataaatac 
      gcacgcgac ggacgtgat ggacacacc cggttggaa 
      gagcatgat ccaaacgca tcctcatct gatcgccga 
      ggcgttccg tgcgctggc gacccagca gatcccccc 
      cataggtcc accacgaaa aagggtgaa acagagctg 
      aagctcgtg atagtgcca cagactgct atcgatgtg 
      ttcac