Status
Please wait ...
Gene information
General information
TC000627 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8884247..8885703
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000627
    iBeetle screen iB_03054

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000627 RA
      MENGDVMLQ CYNKGCGQK FDPSQNKED SCCHHPGAP 
      VFHDAYKGW SCCNKKCTD FTEFLNIKG CTKSKHSNV 
      KPPEPEKPA KREADVNEV IEVKPIVPT AKERPPFDT 
      PMTVMQPEI APSAQQQVL NISNANTAQ NSNEIAIGT 
      TCKNGGCGQ TYQGPETNA TPCTHHPGV PIFHEGMKF 
      WSCCQKRTT DFNAFLNQV GCKSGQHVW IKENADNTT 
      VQCRWDYHQ TATHVVVSI YAKQYCVKK SEIKLNPIR 
      LYASLVFPQ QNNAVFNLD LELRGIVDV GASQVSMYG 
      TKVEIKLKK AEPGSWAKL DIPRVSEEK PKPVTVADI 
      TPQVDAVDL DDL
    CDS sequence
    • >TC000627 RA
      atggaaaac ggtgatgta atgttacag tgttacaac 
      aaaggttgc ggtcaaaaa ttcgaccca agtcagaat 
      aaagaagat tcttgttgc catcaccct ggcgcacct 
      gttttccac gacgcttac aagggctgg tcttgctgt 
      aacaaaaaa tgtacagac tttacagaa tttttaaac 
      ataaagggt tgcacaaaa tcaaaacat tcaaacgtg 
      aagccccca gaacccgaa aaacccgcc aaacgggag 
      gcggacgta aacgaagtg atcgaagtc aaaccaatt 
      gtgccgaca gccaaagaa cggccccca tttgatact 
      ccaatgact gtaatgcaa cctgagatt gcaccttcg 
      gcacaacaa caagtccta aacatctca aatgcgaac 
      acggcgcaa aactcgaac gaaattgcc attggcacc 
      acttgtaag aacgggggc tgcggccag acgtaccaa 
      ggccctgaa acgaacgcc accccctgt acacaccat 
      cccggcgtc cccattttc cacgaaggg atgaagttc 
      tggtcctgc tgtcagaaa cgcaccact gatttcaac 
      gcgtttttg aaccaagtg gggtgcaaa agcggccaa 
      cacgtgtgg attaaggag aatgcggac aacacaacc 
      gtccaatgt cgctgggat taccatcaa actgccact 
      cacgtcgtc gtttccatt tatgcgaaa caatattgc 
      gttaaaaaa agcgaaatt aaactaaat ccgattaga 
      ctttacgcg agtttggtg tttccccaa caaaataat 
      gcggttttt aacttggat ttggagctc agagggatt 
      gttgatgtg ggggcaagt caggtttca atgtatggg 
      accaaagtt gagattaag ttgaagaag gcagaacct 
      gggagttgg gcaaaattg gatatacct agggtgagt 
      gaggagaag cccaagcct gtcacggtc gccgatatt 
      acgccacag gttgatgca gtcgatctt gacgattta 
      taa
    mRNA sequence
    • >TC000627 RA
      acaaagaca agcaattcg ttttgattg gctaaaagc 
      cccaccagg cccgacaac attacaaat acaaataaa 
      tgaatgaaa attctggaa aatctcaca aaagtaacg 
      ttcagtttt agtttaatt tacccaaat ttcgtacag 
      tgagaagat ggaaaacgg tgatgtaat gttacagtg 
      ttacaacaa aggttgcgg tcaaaaatt cgacccaag 
      tcagaataa agaagattc ttgttgcca tcaccctgg 
      cgcacctgt tttccacga cgcttacaa gggctggtc 
      ttgctgtaa caaaaaatg tacagactt tacagaatt 
      tttaaacat aaagggttg cacaaaatc aaaacattc 
      aaacgtgaa gcccccaga acccgaaaa acccgccaa 
      acgggaggc ggacgtaaa cgaagtgat cgaagtcaa 
      accaattgt gccgacagc caaagaacg gcccccatt 
      tgatactcc aatgactgt aatgcaacc tgagattgc 
      accttcggc acaacaaca agtcctaaa catctcaaa 
      tgcgaacac ggcgcaaaa ctcgaacga aattgccat 
      tggcaccac ttgtaagaa cgggggctg cggccagac 
      gtaccaagg ccctgaaac gaacgccac cccctgtac 
      acaccatcc cggcgtccc cattttcca cgaagggat 
      gaagttctg gtcctgctg tcagaaacg caccactga 
      tttcaacgc gtttttgaa ccaagtggg gtgcaaaag 
      cggccaaca cgtgtggat taaggagaa tgcggacaa 
      cacaaccgt ccaatgtcg ctgggatta ccatcaaac 
      tgccactca cgtcgtcgt ttccattta tgcgaaaca 
      atattgcgt taaaaaaag cgaaattaa actaaatcc 
      gattagact ttacgcgag tttggtgtt tccccaaca 
      aaataatgc ggtttttaa cttggattt ggagctcag 
      agggattgt tgatgtggg ggcaagtca ggtttcaat 
      gtatgggac caaagttga gattaagtt gaagaaggc 
      agaacctgg gagttgggc aaaattgga tatacctag 
      ggtgagtga ggagaagcc caagcctgt cacggtcgc 
      cgatattac gccacaggt tgatgcagt cgatcttga 
      cgatttata attttgtaa tgaaatatt taagatttt 
      ttatgaata ttatatgta atttcattt tgcaaccaa 
      cacgaaata tacggtttt gaaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY