Status
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Gene information
General information
TC000586 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9375779..9386786
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000586
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03046

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000586 RA
      MLEGAVARL LNHLLGKYV VDLDTENLN VGIFSGHVQ 
      LTDLKLKTE ALYELGLPI EVKAGTVGK IWLQIPWTC 
      LWSQPVLVN IEDLHIICG PLLSDQPFD AEKNKRLIR 
      AFKRKILAD LDTESHIIG GPNSFSEHL VTNILNCLQ 
      FSVTNVHIR YEDVISYKT PISAGLCIG SVTAETTNS 
      KWKPNKPET NSNTCYYLV KLETLSAYW NSDSKLSKW 
      NLPSEYYVW RNAMSNSLQ NFSINDENF EFVLKPMTT 
      KIKITMNKS NIGNIGKCL IDVIVQDCN LQISKEQYD 
      AILAVSDSL KRILISWDF LASRPHETI LGNTKAWWK 
      YAYFAVLDQ RIRPYSWKK IKNVRDYYR GYVQTYKQI 
      ILNPNDTEL KLDLQKYED NLSVINVVI ARQHARLLV 
      QSRSLSEKN FWSMLPSPE RTLLCKKIG FYDKNCDQL 
      QIDQQYNLR MGNFSLSLT HDSKEIFLL TLTQTNFNF 
      HPNQLEDTF KALVKIEGL ILEGSNEDE QLISILASE 
      HLSTSPAYF FKAELEKMP TNSNASHKL NLALDSVEC 
      LYNQQFFDD LSKWLDNEY LTQTSLFSE INDCPQTIR 
      SFFDEKLQQ KWDLTLSIK IPFMVIPEF GSFLKADNI 
      LVVDLGRYL VTTELCQNS DLIENATQM EMEEQLYSK 
      YHIECTDMQ VLFCQNSDN WKDERKEKD TDLHIIPKT 
      AFSATYACC TRIEKTIPR YKFNINFPT LKLNLSERK 
      SGQILKFFT NNGNYLERG KPNPRPETD FYEHKIIQS 
      YNLKYLSSV ESKVSIKDN FIKIKRGKV DKNQLNKIE 
      KCADNVKKE DMNEAWARY VDLPGLEDN ISPNNNITV 
      LLRFIINEF TLVFSRSSD STDRQYLMF RLGLLTTDV 
      ALMTYGPAY QISVNSVLL TDKLHTTPS GQYLDLVHS 
      PFPSTVDVI TILYRKVSA SCPDFWSHF HGVETSLVA 
      DFGTIHLLL HQEAIHTLV KYSNYLFNK LKLQTPLSI 
      RDAALKSLK LINNVLHQQ PVTPVPPGS VKFSHSARF 
      ADVNVRVCD SDFDIINIQ LSGLEMDFL FRANERFVF 
      RSYLSNINV EHLSEVTLY SKVLSTDED KVFELKYVR 
      HASHINQNE ISNREEMTT GGSFKFQLG QIHLTLLYK 
      LIVQIQRFV TNLEAVPYI NNIIEYLYN AVTTVTDTL 
      KANTKIHLA INVCGPILL FPQKSSSPN VMIIDTGEL 
      HVENFFKEY NSDTMENIL IKWTGTTIT RGVMTLIPS 
      LEMQETLIE PINLNFDIK RYTNIKSAQ KFWDIDGAL 
      DPIQVTLGQ RDISTILSI YKDNIGEGK FVDLFPVQI 
      KSPLTSAIT DETVKTLEA FFCEPKQKN IVAKFSLDE 
      ITLLLFFDS GELLSSPIR DLNHGLCKF KVSEVNTTF 
      TIYTDGSLD GKLSADGIL VEEIGPDAN IYDKRILQS 
      PVDDNKNNN CNITINRAP IVDITFHQN KSGDNSADV 
      IIGRLSLSL SVPFCEKLA LFVLECLPK DNSDIGIVN 
      HGYECDTHE EKEYAASLT ISLRINKPE FIFVVETTS 
      NKKRYFITH TEILSDYSR HGNRLNLVV SLSGLHSLF 
      YDIGVQSNE PYVILKQCD VEFSKCYSE EKGKKIIAS 
      ISSVYVQIC SRVVHSLSD ILNDISEHF KVPELEPRD 
      AVTKLEEDL WESKKMDDF VQPKPDSTL DNKLVAKEV 
      ELHEILLVP KFDIVLIFE LEDTQVLLV KSTMEVTVY 
      DWSSLLNCT CEVTIQANY FNENLQVWE PVVDPVVID 
      EKEYRPWDV LVKIFQDKS LPMLSSLDQ KSRNATREK 
      KSTPTTTED EDSGEDMMY LEPINPLHN RHNRRVKTS 
      LSTFLDDSD SENEDGAME KLAAAISDL FTGDWNESE 
      DSDYIHSSD NEDDSDDNV KRKSQDDKM SQFASYTKS 
      TYVLIDAKE VLNVTITPT FLKVMNELL TIYSNKTLS 
      VITNKKTIN LTNDIGPQS KVELYEKKS VESYEEDAL 
      ICVKTFENQ DSVPNSPSK SVYYVSDFS EDVNEDKDS 
      TQGDVFKAD LENNYDFAT MSSLQFPSE TTPQLYDKI 
      NAHFMRVFI PHCTPIQTN CSKRAWQKL MRLHSTIPG 
      QKYYLAAKH TISKSGRNV VISSPLQIK NETCFALSV 
      LYQPSVLQQ LNLEPVGDV TNPFETTMR IAVLEAHEH 
      YNVPLYIAY HCKLFIQPA YAEGHYTSD CGIWWKDLA 
      TELDIAHNL HCRPRTDSN LEVFSLRVM LTRNLDIPN 
      SQAHTVPNY VIHLLPPLI FHNYLPYSL EVENVGLKQ 
      LIKIEPGEK KSVYSLDLS KDQKLLIRV KYNLLTWSG 
      ILNFTTYLD EKIVVLSSD SKEIKHLAI NTKTDREGS 
      CNMFFYTPY WIINKIGLP LQIKASATN TVYDSNSED 
      ILLFTYKRH GKQTLSVKV YDSNWSNEF GLECAGTTG 
      LIICKDNER KKKYLFFLS IKLSQMCPR LTKIVTLLP 
      TFLITNNTN KKLRFMEHN EKTDLWIDL ESSQTVTFW 
      PETSSMQMY VKYRDSKVI SQAFNFATP NSTVLRMDK 
      GGALKVEVT GGISDSFRV TFYEYKSGD APVLVKNYC 
      PDLFLKIQQ QDQSQVTLL SPYNSLLYT WDDPTRIRK 
      LAWNVYNNK GAGFVLDIC KDSHGEEKI LFHSVTPTT 
      SVTISSSED SDSSDSVKT TMNKKVRRD KIVIYWLCY 
      ADGLQRTLL FTQESRIYN TVLKHYFLE HCDLECLVS 
      LCGVGVSLF TSENAKKEH VYGSLVDSP AIWEVNVGH 
      KWKTLTLEL ASWIEDKYK LHYKKCQLR DYIHIDFEK 
      MYMLKPFFA ELKRTYTPA VYLQYRKSK NYNHFNFKL 
      NTLQIDNKV NNTIVLYPL PGNVTKGLN SFIDVNVLK 
      CLAKDCDIY RHIKVNIKD FYLNIENDL FVDIRELLV 
      QYKKFYDET NISYVNDIK SIQDLTLVR SKDPQTGRS 
      LIEYLSVSS FEIQLHISN KTQLILKSS KLPLCKILD 
      YLFPINISP YMPLEGVLQ KVSAIEQTN LCDHLSTCL 
      ENLLQQIIA QFLQQYYSH VLGLQVLVN TFAIQPAIL 
      YPQIDLEKM SNTVLYGSR CLLSHINMS PAALEACVV 
      DIFAHQTIE NIQRIRRHG SYQKSEVVP KIITTSCRN 
      FHTGVPNAL NQLIVRKQN AGINCDGEM FFRTTGKAL 
      FSLMTRHPD EKSDSVEVA KEALRRASI LGEPIRIQQ 
      RLTRYYNRY LGLKPFSVY ESMGQYLLE TVANSRFMK 
      DTYWSHASI DRSGKSVLI VSLQHVVRI NKCRLWGPW 
      DVEWAIDLD DILSMPKIT STELVFNMR QSENNTREL 
      LLTISGDKE MLTWIHEKI EQAIIVSME DKSWPVAEN 
      P
    CDS sequence
    • >TC000586 RA
      atgctcgaa ggggccgta gcgagactc ctgaatcat 
      ttattgggc aaatatgtg gtggacctt gacactgaa 
      aacctaaat gttggaata ttttcagga catgtgcag 
      ttgactgat ttgaaatta aaaacagag gctttgtac 
      gagttaggt cttccaatc gaagtcaaa gccggcact 
      gttggaaaa atatggctg cagataccc tggacgtgt 
      ctgtggagc caaccagtt ctggtcaac atcgaggac 
      ttgcacatt atttgtggt cccttgctc tcagaccaa 
      cctttcgat gccgaaaaa aacaaacgg ctgattcga 
      gcatttaag cgcaaaatt ttggctgat ttggacacc 
      gaaagccac attatcggc ggccccaac tctttttcc 
      gaacattta gttacaaat atcttgaat tgtttgcaa 
      tttagtgtt actaacgtc cacatcaga tacgaagac 
      gtaattagc tataaaacg ccgatttcg gccggtttg 
      tgcattggg agtgtcaca gcggaaacg acaaatagt 
      aaatggaag cccaacaaa cctgaaact aacagcaac 
      acatgctac tacctagtc aagcttgaa acgctgtcc 
      gcttactgg aactcggac tcgaaattg tcaaagtgg 
      aatctaccc tcggaatat tacgtttgg aggaacgcc 
      atgtcgaac agtttgcaa aatttttca atcaacgac 
      gagaatttc gaatttgtt ctgaagcca atgacgact 
      aaaataaaa ataacgatg aacaaatct aatattgga 
      aatatcggc aaatgttta attgatgtg atagtgcaa 
      gactgtaat ttacaaatt tccaaggaa caatacgac 
      gcaattttg gcagtaagc gactccctg aaacgtatt 
      ttaatcagt tgggatttc ctggcctca cggccgcac 
      gaaacaatt ttaggaaac actaaagct tggtggaag 
      tacgcctat tttgcggtt ttggaccaa agaattcgc 
      ccctattct tggaaaaaa attaaaaat gttagagat 
      tattaccgc ggatacgtg caaacctac aagcagatt 
      attctgaat ccgaacgat acggaattg aagctagat 
      ttgcaaaaa tatgaagac aatttatcc gttattaat 
      gttgtgata gcaaggcaa catgcacgc cttttggtg 
      caatcgcgt agtttaagc gagaaaaat ttctggtca 
      atgttaccc tcacctgaa cgtactttg ctgtgtaaa 
      aaaatcggg ttctacgat aaaaactgt gaccagtta 
      cagatagat caacagtat aatctacga atgggtaat 
      tttagcttg tcgctgaca cacgattcc aaagaaatt 
      tttcttcta actctaact cagacgaat ttcaacttc 
      cacccaaat caattggaa gacactttt aaggcactg 
      gttaaaatc gagggcttg attttggaa ggttcaaac 
      gaagacgaa caattaatc tcgatcctg gcctccgag 
      catcttagt acaagtccg gcgtatttt tttaaagca 
      gaattggaa aaaatgccg acaaattca aacgcttca 
      cataaattg aatctagct ttagattcg gtcgagtgt 
      ttgtataac caacaattt ttcgacgac ctttccaag 
      tggctagat aacgaatac ttgacacaa acgtccctt 
      ttcagcgaa attaatgac tgcccacag acaatacgt 
      agtttcttc gacgaaaag ttgcaacaa aagtgggac 
      ttgaccctt agtattaaa atcccgttc atggtgata 
      cccgaattc gggtctttc ctaaaagca gataatatt 
      ctagtcgtg gatttaggc cgttattta gtcacgacg 
      gaactttgc caaaatagt gacttaatc gaaaacgcg 
      actcaaatg gaaatggaa gaacagctc tactcgaaa 
      taccacatc gaatgcacc gacatgcag gtccttttt 
      tgccaaaac tccgacaac tggaaggac gaaaggaag 
      gaaaaagac accgacttg cacataatc cccaaaacg 
      gccttttcc gccacgtac gcctgttgc actcgaatc 
      gaaaaaacc atcccgaga tataaattc aacatcaat 
      ttccccacc cttaaattg aatttatcg gagcggaaa 
      agcggccaa atcctcaag tttttcaca aacaatggg 
      aactaccta gaaaggggg aagccaaac ccgcgacca 
      gaaaccgat ttttatgaa cacaaaatc atccaaagc 
      tacaatctc aagtatttg tccagtgtc gagtcaaag 
      gtttcgatc aaggacaat ttcatcaaa attaagcga 
      gggaaagtc gacaagaac caactgaat aaaatcgag 
      aaatgtgcc gataatgtt aaaaaagag gacatgaac 
      gaggcttgg gcccgctac gtggatttg cccggctta 
      gaggacaat atttcgccc aataacaac ataacagtg 
      ctccttaga ttcatcatc aatgaattc actcttgtc 
      ttctcaaga tcaagcgat agtaccgac cgccagtac 
      ctaatgttc cgactcggg cttttgacc acagatgtg 
      gccctaatg acttatggg ccagcttac caaatctca 
      gtcaacagt gttttacta acggataaa ttgcacaca 
      acacccagt ggacaatat ttggattta gtccatagc 
      ccatttccc agcactgtt gacgttatc acaatcctg 
      tacaggaaa gttagcgcg agttgtccc gacttttgg 
      agccacttt cacggtgtt gagacctca ctagtggcc 
      gacttcgga accatccac ttgttactc caccaggaa 
      gctattcat accctcgtc aaatactcg aattatttg 
      ttcaataaa cttaaacta caaacgcct ttgtcaatc 
      cgtgacgct gcgttgaaa tcgttgaaa ttgatcaat 
      aatgtcttg catcagcaa ccagtgact cccgttcct 
      ccaggatcg gtcaaattc tctcattcg gcgagattc 
      gcagatgtg aacgttcga gtgtgtgac tctgatttt 
      gacatcatc aacatacag ttatcagga ctcgaaatg 
      gatttcctg ttcagggct aacgagcga tttgtgttc 
      aggtcatac ttgagtaac attaacgtg gagcacttg 
      tccgaagtc acactttat agtaaagtg ttatcaacc 
      gatgaggat aaagttttc gagctcaaa tacgtccga 
      catgcctct cacattaac cagaatgag atttcaaac 
      cgggaggag atgaccact ggcggcagt ttcaagttc 
      caactcgga caaatccac ctaactctc ttgtataaa 
      ttaatagtg caaatccaa cgatttgtc acaaatttg 
      gaggccgtc ccttacata aataacata atagagtac 
      ctctacaat gctgtaact acagtcact gacacacta 
      aaagccaac acaaaaatc caccttgcg ataaacgtc 
      tgtggcccg atcctgctc ttcccccag aaatcctca 
      tcccccaat gtaatgata atcgacact ggcgaactc 
      cacgtggag aacttcttc aaggagtac aacagcgac 
      accatggag aacattttg atcaaatgg accggcacc 
      acgattact cggggcgta atgacccta ataccgtcc 
      ctcgaaatg caggaaacc ctaatcgag ccaataaac 
      ctcaacttc gacatcaaa cgctacacg aatatcaaa 
      agcgcgcag aagttctgg gacatcgac ggggctctg 
      gaccccatc caggtcacc ctcggccaa agagacatc 
      tccacgatc ttatcaatc tacaaagac aacattggc 
      gagggcaaa ttcgtcgac cttttcccc gtccagatc 
      aaatcgccc ctcacttcc gcaatcacc gatgagacg 
      gttaaaacc ctcgaggcg tttttctgc gaacctaag 
      cagaaaaac atagtcgcg aaattttcg ctcgacgaa 
      atcacgttg ttgctgttt ttcgattca ggggagttg 
      ctaagttcg ccgattcgt gacttgaac cacggcttg 
      tgcaagttt aaagtgtcg gaagttaac acaactttc 
      acgatttat actgacggg agtctggat gggaaattg 
      tcggctgat gggattctg gttgaggag atcgggccc 
      gatgccaac atttacgat aagaggatt ctccagtcg 
      ccggttgat gataacaag aataataac tgtaatatt 
      acgataaat cgggccccg attgtggat atcacgttc 
      catcagaac aaatcgggg gataattcg gccgatgtg 
      atcattggc aggttgagt ttgagtttg tcggtgccg 
      ttctgcgag aaactggcc ctttttgtg cttgagtgt 
      cttccgaag gacaattcc gacatcggg attgtgaat 
      cacggctat gagtgtgac acgcatgag gagaaagaa 
      tacgcagcg agtctcaca atttcgctg aggattaac 
      aaaccggag tttatattc gttgtggag accacgtcc 
      aacaaaaaa cgctatttc atcacccac actgaaatt 
      ttgtcagac tattccaga cacggaaat cggctaaac 
      ctagtcgtg tctttatca ggactacat tctctcttt 
      tacgacatt ggcgtgcaa tctaacgag ccttacgtc 
      atactgaaa cagtgcgac gttgaattt tccaagtgt 
      tattccgaa gagaaaggc aagaaaatc atcgcgtcc 
      atatcctcg gtctacgtg cagatttgc agccgagtc 
      gtccactcc ctaagcgac atcctgaac gacatctcc 
      gaacacttc aaagtgcct gagcttgaa ccccgagac 
      gctgttacc aaactcgag gaagaccta tgggagtcg 
      aaaaaaatg gacgatttc gtgcaacca aaacccgac 
      tcaactctc gataacaaa ctagtggcc aaagaagtc 
      gaattgcat gaaatcctc ctcgtcccg aaattcgac 
      attgtgctg attttcgaa ctggaggac acgcaagtg 
      cttctggtg aagtcaaca atggaggtg acggtctac 
      gactggtcc tcgctccta aactgcacg tgcgaggtg 
      acaatccaa gccaattac ttcaacgag aacctgcaa 
      gtttgggag ccggtggtc gaccccgtg gtcatagac 
      gagaaggag tacagacca tgggacgtc ctggtcaag 
      attttccag gataagtcg ttgcccatg ttgagcagt 
      ctggaccaa aagtcgcgg aacgccacc cgggagaag 
      aaaagtacg cccaccacg accgaagac gaggattcg 
      ggagaggac atgatgtac ctcgagccg atcaacccg 
      ctccacaac cgccacaac aggagggtc aagacaagt 
      ctctccaca tttctggac gactcggat tcggaaaat 
      gaggacggg gcgatggaa aaactcgcc gccgcgatt 
      tcagaccta ttcaccggc gattggaac gaaagcgaa 
      gacagtgac tacatccac tcgagcgac aacgaagac 
      gattcggac gacaatgtc aaaaggaaa tcacaagac 
      gataaaatg agccaattt gcgagctac acgaaatcg 
      acttatgtc ctgattgac gcaaaggaa gtcctaaat 
      gtgaccata accccgact tttctcaaa gttatgaac 
      gaactttta accatctac tcgaacaaa accctctcg 
      gtgatcacc aacaagaaa acgatcaac ttaacgaac 
      gacatcggc ccgcaatcg aaagtcgag ctctatgag 
      aagaaaagt gtggagagt tacgaggag gacgcgctg 
      atttgcgtc aaaaccttc gaaaatcag gactctgtg 
      cccaacagt cccagcaaa agcgtttac tacgtctcg 
      gatttttca gaggacgtg aatgaggat aaagacagc 
      acgcaaggg gatgtgttt aaggccgac ttggagaac 
      aattacgac tttgcgacc atgtcgagc ctccagttc 
      ccctcagag acgactccg caactgtac gacaaaatc 
      aacgctcat tttatgcga gttttcatc ccgcattgt 
      accccgatt cagacgaat tgttcgaaa cgagcttgg 
      caaaagctt atgagactg cactcgact atcccaggc 
      cagaagtac taccttgcc gccaaacac acgataagc 
      aaatcgggc agaaacgtt gtcataagc tcgccgtta 
      caaatcaaa aacgagact tgttttgcc ttgagtgtc 
      ttataccaa ccgtcggtt ttgcaacag ttgaattta 
      gagccggtg ggcgacgtc acaaatccc tttgagaca 
      actatgagg attgcagtt ttggaagcg cacgagcat 
      tacaatgtg ccgctctat atcgcctac cattgcaag 
      ctttttatc caaccagct tatgccgag ggacattac 
      acctccgac tgtggaatt tggtggaag gatttagcc 
      acggagctc gacatcgcg cataatttg cactgcagg 
      ccgcgaact gactcaaat ttggaggtt ttttcgttg 
      cgggtgatg ctgacccga aacttggac ataccaaac 
      tctcaagcg cacactgtt ccgaactat gtcatacat 
      ttattgccc ccgttaatt ttccataat tacctccct 
      tactcgctc gaagtcgaa aatgtcggc ttgaaacaa 
      ctgattaaa atcgaaccc ggggagaaa aagagtgtc 
      tactcgttg gatttgtcc aaagatcag aaactacta 
      atccgagtc aagtataat ttactcact tggagcgga 
      attttgaat tttacaacg tacctcgac gaaaaaatc 
      gtagttttg tcgtcagac agtaaagaa atcaaacac 
      ttggccatc aacaccaaa acggatcgc gagggaagc 
      tgtaacatg tttttttac accccgtac tggattatt 
      aacaaaatt ggattgccg ttacaaatc aaagcctca 
      gcgacgaac acagtctat gacagtaac tccgaagat 
      atccttctt ttcacgtat aaaagacac ggaaagcaa 
      actttgagc gtgaaagtt tacgactcg aattggtca 
      aacgaattc gggctagag tgtgccgga accaccggt 
      ttaataatt tgcaaggac aacgaacga aagaagaaa 
      tatttgttc tttttgtcc atcaaactg tcacaaatg 
      tgtccaaga ttgacgaaa atcgttact cttttgccc 
      acttttctc atcaccaat aacactaac aaaaaattg 
      agatttatg gaacacaat gagaaaacc gatctctgg 
      atcgacttg gagtcgtcc caaaccgtg actttctgg 
      cctgaaacc tcctcaatg caaatgtac gtcaaatac 
      agggacagt aaagtgatt tcgcaggct tttaatttc 
      gcaactccg aattcaaca gtcctccgt atggataag 
      ggaggagct ttgaaagtt gaagttacg ggaggcata 
      agtgactca ttccgtgtc acgttttac gagtataag 
      tctggcgac gcccccgtt ttggtcaaa aactactgt 
      cccgaccta tttttgaag atccagcag caggaccaa 
      agccaggtc actttgctc agcccttac aattccctc 
      ttgtacact tgggatgac ccaacaagg atcaggaag 
      ctggcttgg aacgtttac aacaataaa ggagcagga 
      tttgtcctt gacatttgc aaggactca cacggagag 
      gagaaaatt ctcttccac agcgtcaca cccaccact 
      agtgtgacc atttcgtcg agcgaggac tcggatagt 
      tccgacagt gttaaaaca acaatgaat aaaaaagtc 
      cgaagggac aaaatcgtg atttactgg ctttgctac 
      gccgacggg cttcaaaga acgctgctc ttcacccaa 
      gaatcgcgg atttataac acggttttg aaacactat 
      tttttggaa cattgcgac ttggagtgt ttagtctcg 
      ctctgcggt gtcggagtt tcccttttc acctccgaa 
      aatgcgaaa aaagagcac gtttatggc tcgcttgtt 
      gacagtccg gccatttgg gaagtgaac gttggccat 
      aaatggaaa actctgact ttggaattg gcttcgtgg 
      atcgaggat aagtacaag ttgcattac aagaaatgc 
      cagttgcgg gattacata cacatcgat tttgaaaaa 
      atgtacatg ttgaagcct ttcttcgcc gaattgaaa 
      cgcacgtac acgcctgcg gtttatctc cagtacagg 
      aagtccaaa aattacaat catttcaat ttcaaacta 
      aacacactg cagattgat aacaaggtc aataatacg 
      attgttttg tacccactt ccagggaat gtcacaaaa 
      ggattgaac tctttcatc gacgtcaat gttttgaag 
      tgtttggcg aaagattgc gatatttac agacacatc 
      aaagtcaat attaaggat ttctatttg aacatcgaa 
      aacgatttg tttgtggat attcgggaa cttttggtg 
      cagtataaa aagttttat gatgagact aatatttcg 
      tacgttaat gatatcaag tcaatccaa gatttgact 
      cttgtgcgg tctaaagat ccccaaact ggtcgaagt 
      ttgatcgaa tatttaagt gtgagcagc ttcgaaatc 
      cagttacac atctctaat aaaacacaa ttgatttta 
      aaaagcagc aaactgcct ttgtgcaaa attttggac 
      tatttgttt cccataaac atttcgccc tatatgcct 
      ctggaaggc gtccttcaa aaggtttca gctatcgaa 
      caaaccaat ctctgcgac cacctctcc acatgtctg 
      gaaaaccta ctccaacaa ataatagcg caatttctg 
      caacagtac tattcccac gtcctcggt ttgcaggtt 
      ttggtcaac accttcgcc atccaacct gcgattttg 
      tacccgcaa atcgatttg gaaaaaatg tcaaacacg 
      gttttgtac ggctcgaga tgtcttttg agtcacatt 
      aacatgtct cccgcggct ttagaagct tgcgttgtc 
      gatattttc gctcatcaa acgattgaa aatattcag 
      aggatccgg agacacggc tcgtaccaa aagtcggag 
      gtggtaccg aaaatcata actacgtct tgtcgcaac 
      ttccacacc ggggttcca aacgcattg aaccaattg 
      attgtgaga aaacaaaac gctgggatt aattgtgac 
      ggcgaaatg ttcttcaga acgaccgga aaagcgctg 
      ttttccctc atgacgcgt caccctgac gagaaaagt 
      gacagtgtc gaagtggcc aaagaggcc ttgagacga 
      gcctcgatt ttaggggaa ccgattcgg attcagcaa 
      cgtcttacc agatattac aaccgatat ttgggcctt 
      aaaccgttc tcagtgtac gaatctatg ggacagtat 
      ttgctagaa actgttgct aattctaga tttatgaaa 
      gacacctac tggtcgcat gcttcgatt gatagaagc 
      ggcaaatca gttttgata gtttcgcta cagcacgtt 
      gtacgaata aataagtgc cggctttgg ggaccgtgg 
      gatgttgag tgggctata gacttggat gatatttta 
      tcgatgcca aaaattacg tctaccgag cttgtcttt 
      aatatgaga cagagcgaa aataatacg agagaattg 
      ttgttgact atcagcggt gataaagaa atgctaact 
      tggatccac gaaaaaatt gagcaagct attatagtt 
      agtatggaa gataaatcc tggcccgtt gctgaaaat 
      ccataa
    mRNA sequence
    • >TC000586 RA
      agagttgct ttcacaact gctaaacca aaccattcg 
      acattaaaa atcagccta aatcaatag ttttccctg 
      atatgattt aaaaaaaca cctcgacaa gatgctcga 
      aggggccgt agcgagact cctgaatca tttattggg 
      caaatatgt ggtggacct tgacactga aaacctaaa 
      tgttggaat attttcagg acatgtgca gttgactga 
      tttgaaatt aaaaacaga ggctttgta cgagttagg 
      tcttccaat cgaagtcaa agccggcac tgttggaaa 
      aatatggct gcagatacc ctggacgtg tctgtggag 
      ccaaccagt tctggtcaa catcgagga cttgcacat 
      tatttgtgg tcccttgct ctcagacca acctttcga 
      tgccgaaaa aaacaaacg gctgattcg agcatttaa 
      gcgcaaaat tttggctga tttggacac cgaaagcca 
      cattatcgg cggccccaa ctctttttc cgaacattt 
      agttacaaa tatcttgaa ttgtttgca atttagtgt 
      tactaacgt ccacatcag atacgaaga cgtaattag 
      ctataaaac gccgatttc ggccggttt gtgcattgg 
      gagtgtcac agcggaaac gacaaatag taaatggaa 
      gcccaacaa acctgaaac taacagcaa cacatgcta 
      ctacctagt caagcttga aacgctgtc cgcttactg 
      gaactcgga ctcgaaatt gtcaaagtg gaatctacc 
      ctcggaata ttacgtttg gaggaacgc catgtcgaa 
      cagtttgca aaatttttc aatcaacga cgagaattt 
      cgaatttgt tctgaagcc aatgacgac taaaataaa 
      aataacgat gaacaaatc taatattgg aaatatcgg 
      caaatgttt aattgatgt gatagtgca agactgtaa 
      tttacaaat ttccaagga acaatacga cgcaatttt 
      ggcagtaag cgactccct gaaacgtat tttaatcag 
      ttgggattt cctggcctc acggccgca cgaaacaat 
      tttaggaaa cactaaagc ttggtggaa gtacgccta 
      ttttgcggt tttggacca aagaattcg cccctattc 
      ttggaaaaa aattaaaaa tgttagaga ttattaccg 
      cggatacgt gcaaaccta caagcagat tattctgaa 
      tccgaacga tacggaatt gaagctaga tttgcaaaa 
      atatgaaga caatttatc cgttattaa tgttgtgat 
      agcaaggca acatgcacg ccttttggt gcaatcgcg 
      tagtttaag cgagaaaaa tttctggtc aatgttacc 
      ctcacctga acgtacttt gctgtgtaa aaaaatcgg 
      gttctacga taaaaactg tgaccagtt acagataga 
      tcaacagta taatctacg aatgggtaa ttttagctt 
      gtcgctgac acacgattc caaagaaat ttttcttct 
      aactctaac tcagacgaa tttcaactt ccacccaaa 
      tcaattgga agacacttt taaggcact ggttaaaat 
      cgagggctt gattttgga aggttcaaa cgaagacga 
      acaattaat ctcgatcct ggcctccga gcatcttag 
      tacaagtcc ggcgtattt ttttaaagc agaattgga 
      aaaaatgcc gacaaattc aaacgcttc acataaatt 
      gaatctagc tttagattc ggtcgagtg tttgtataa 
      ccaacaatt tttcgacga cctttccaa gtggctaga 
      taacgaata cttgacaca aacgtccct tttcagcga 
      aattaatga ctgcccaca gacaatacg tagtttctt 
      cgacgaaaa gttgcaaca aaagtggga cttgaccct 
      tagtattaa aatcccgtt catggtgat acccgaatt 
      cgggtcttt cctaaaagc agataatat tctagtcgt 
      ggatttagg ccgttattt agtcacgac ggaactttg 
      ccaaaatag tgacttaat cgaaaacgc gactcaaat 
      ggaaatgga agaacagct ctactcgaa ataccacat 
      cgaatgcac cgacatgca ggtcctttt ttgccaaaa 
      ctccgacaa ctggaagga cgaaaggaa ggaaaaaga 
      caccgactt gcacataat ccccaaaac ggccttttc 
      cgccacgta cgcctgttg cactcgaat cgaaaaaac 
      catcccgag atataaatt caacatcaa tttccccac 
      ccttaaatt gaatttatc ggagcggaa aagcggcca 
      aatcctcaa gtttttcac aaacaatgg gaactacct 
      agaaagggg gaagccaaa cccgcgacc agaaaccga 
      tttttatga acacaaaat catccaaag ctacaatct 
      caagtattt gtccagtgt cgagtcaaa ggtttcgat 
      caaggacaa tttcatcaa aattaagcg agggaaagt 
      cgacaagaa ccaactgaa taaaatcga gaaatgtgc 
      cgataatgt taaaaaaga ggacatgaa cgaggcttg 
      ggcccgcta cgtggattt gcccggctt agaggacaa 
      tatttcgcc caataacaa cataacagt gctccttag 
      attcatcat caatgaatt cactcttgt cttctcaag 
      atcaagcga tagtaccga ccgccagta cctaatgtt 
      ccgactcgg gcttttgac cacagatgt ggccctaat 
      gacttatgg gccagctta ccaaatctc agtcaacag 
      tgttttact aacggataa attgcacac aacacccag 
      tggacaata tttggattt agtccatag cccatttcc 
      cagcactgt tgacgttat cacaatcct gtacaggaa 
      agttagcgc gagttgtcc cgacttttg gagccactt 
      tcacggtgt tgagacctc actagtggc cgacttcgg 
      aaccatcca cttgttact ccaccagga agctattca 
      taccctcgt caaatactc gaattattt gttcaataa 
      acttaaact acaaacgcc tttgtcaat ccgtgacgc 
      tgcgttgaa atcgttgaa attgatcaa taatgtctt 
      gcatcagca accagtgac tcccgttcc tccaggatc 
      ggtcaaatt ctctcattc ggcgagatt cgcagatgt 
      gaacgttcg agtgtgtga ctctgattt tgacatcat 
      caacataca gttatcagg actcgaaat ggatttcct 
      gttcagggc taacgagcg atttgtgtt caggtcata 
      cttgagtaa cattaacgt ggagcactt gtccgaagt 
      cacacttta tagtaaagt gttatcaac cgatgagga 
      taaagtttt cgagctcaa atacgtccg acatgcctc 
      tcacattaa ccagaatga gatttcaaa ccgggagga 
      gatgaccac tggcggcag tttcaagtt ccaactcgg 
      acaaatcca cctaactct cttgtataa attaatagt 
      gcaaatcca acgatttgt cacaaattt ggaggccgt 
      cccttacat aaataacat aatagagta cctctacaa 
      tgctgtaac tacagtcac tgacacact aaaagccaa 
      cacaaaaat ccaccttgc gataaacgt ctgtggccc 
      gatcctgct cttccccca gaaatcctc atcccccaa 
      tgtaatgat aatcgacac tggcgaact ccacgtgga 
      gaacttctt caaggagta caacagcga caccatgga 
      gaacatttt gatcaaatg gaccggcac cacgattac 
      tcggggcgt aatgaccct aataccgtc cctcgaaat 
      gcaggaaac cctaatcga gccaataaa cctcaactt 
      cgacatcaa acgctacac gaatatcaa aagcgcgca 
      gaagttctg ggacatcga cggggctct ggaccccat 
      ccaggtcac cctcggcca aagagacat ctccacgat 
      cttatcaat ctacaaaga caacattgg cgagggcaa 
      attcgtcga ccttttccc cgtccagat caaatcgcc 
      cctcacttc cgcaatcac cgatgagac ggttaaaac 
      cctcgaggc gtttttctg cgaacctaa gcagaaaaa 
      catagtcgc gaaattttc gctcgacga aatcacgtt 
      gttgctgtt tttcgattc aggggagtt gctaagttc 
      gccgattcg tgacttgaa ccacggctt gtgcaagtt 
      taaagtgtc ggaagttaa cacaacttt cacgattta 
      tactgacgg gagtctgga tgggaaatt gtcggctga 
      tgggattct ggttgagga gatcgggcc cgatgccaa 
      catttacga taagaggat tctccagtc gccggttga 
      tgataacaa gaataataa ctgtaatat tacgataaa 
      tcgggcccc gattgtgga tatcacgtt ccatcagaa 
      caaatcggg ggataattc ggccgatgt gatcattgg 
      caggttgag tttgagttt gtcggtgcc gttctgcga 
      gaaactggc cctttttgt gcttgagtg tcttccgaa 
      ggacaattc cgacatcgg gattgtgaa tcacggcta 
      tgagtgtga cacgcatga ggagaaaga atacgcagc 
      gagtctcac aatttcgct gaggattaa caaaccgga 
      gtttatatt cgttgtgga gaccacgtc caacaaaaa 
      acgctattt catcaccca cactgaaat tttgtcaga 
      ctattccag acacggaaa tcggctaaa cctagtcgt 
      gtctttatc aggactaca ttctctctt ttacgacat 
      tggcgtgca atctaacga gccttacgt catactgaa 
      acagtgcga cgttgaatt ttccaagtg ttattccga 
      agagaaagg caagaaaat catcgcgtc catatcctc 
      ggtctacgt gcagatttg cagccgagt cgtccactc 
      cctaagcga catcctgaa cgacatctc cgaacactt 
      caaagtgcc tgagcttga accccgaga cgctgttac 
      caaactcga ggaagacct atgggagtc gaaaaaaat 
      ggacgattt cgtgcaacc aaaacccga ctcaactct 
      cgataacaa actagtggc caaagaagt cgaattgca 
      tgaaatcct cctcgtccc gaaattcga cattgtgct 
      gattttcga actggagga cacgcaagt gcttctggt 
      gaagtcaac aatggaggt gacggtcta cgactggtc 
      ctcgctcct aaactgcac gtgcgaggt gacaatcca 
      agccaatta cttcaacga gaacctgca agtttggga 
      gccggtggt cgaccccgt ggtcataga cgagaagga 
      gtacagacc atgggacgt cctggtcaa gattttcca 
      ggataagtc gttgcccat gttgagcag tctggacca 
      aaagtcgcg gaacgccac ccgggagaa gaaaagtac 
      gcccaccac gaccgaaga cgaggattc gggagagga 
      catgatgta cctcgagcc gatcaaccc gctccacaa 
      ccgccacaa caggagggt caagacaag tctctccac 
      atttctgga cgactcgga ttcggaaaa tgaggacgg 
      ggcgatgga aaaactcgc cgccgcgat ttcagacct 
      attcaccgg cgattggaa cgaaagcga agacagtga 
      ctacatcca ctcgagcga caacgaaga cgattcgga 
      cgacaatgt caaaaggaa atcacaaga cgataaaat 
      gagccaatt tgcgagcta cacgaaatc gacttatgt 
      cctgattga cgcaaagga agtcctaaa tgtgaccat 
      aaccccgac ttttctcaa agttatgaa cgaactttt 
      aaccatcta ctcgaacaa aaccctctc ggtgatcac 
      caacaagaa aacgatcaa cttaacgaa cgacatcgg 
      cccgcaatc gaaagtcga gctctatga gaagaaaag 
      tgtggagag ttacgagga ggacgcgct gatttgcgt 
      caaaacctt cgaaaatca ggactctgt gcccaacag 
      tcccagcaa aagcgttta ctacgtctc ggatttttc 
      agaggacgt gaatgagga taaagacag cacgcaagg 
      ggatgtgtt taaggccga cttggagaa caattacga 
      ctttgcgac catgtcgag cctccagtt cccctcaga 
      gacgactcc gcaactgta cgacaaaat caacgctca 
      ttttatgcg agttttcat cccgcattg taccccgat 
      tcagacgaa ttgttcgaa acgagcttg gcaaaagct 
      tatgagact gcactcgac tatcccagg ccagaagta 
      ctaccttgc cgccaaaca cacgataag caaatcggg 
      cagaaacgt tgtcataag ctcgccgtt acaaatcaa 
      aaacgagac ttgttttgc cttgagtgt cttatacca 
      accgtcggt tttgcaaca gttgaattt agagccggt 
      gggcgacgt cacaaatcc ctttgagac aactatgag 
      gattgcagt tttggaagc gcacgagca ttacaatgt 
      gccgctcta tatcgccta ccattgcaa gctttttat 
      ccaaccagc ttatgccga gggacatta cacctccga 
      ctgtggaat ttggtggaa ggatttagc cacggagct 
      cgacatcgc gcataattt gcactgcag gccgcgaac 
      tgactcaaa tttggaggt tttttcgtt gcgggtgat 
      gctgacccg aaacttgga cataccaaa ctctcaagc 
      gcacactgt tccgaacta tgtcataca tttattgcc 
      cccgttaat tttccataa ttacctccc ttactcgct 
      cgaagtcga aaatgtcgg cttgaaaca actgattaa 
      aatcgaacc cggggagaa aaagagtgt ctactcgtt 
      ggatttgtc caaagatca gaaactact aatccgagt 
      caagtataa tttactcac ttggagcgg aattttgaa 
      ttttacaac gtacctcga cgaaaaaat cgtagtttt 
      gtcgtcaga cagtaaaga aatcaaaca cttggccat 
      caacaccaa aacggatcg cgagggaag ctgtaacat 
      gttttttta caccccgta ctggattat taacaaaat 
      tggattgcc gttacaaat caaagcctc agcgacgaa 
      cacagtcta tgacagtaa ctccgaaga tatccttct 
      tttcacgta taaaagaca cggaaagca aactttgag 
      cgtgaaagt ttacgactc gaattggtc aaacgaatt 
      cgggctaga gtgtgccgg aaccaccgg tttaataat 
      ttgcaagga caacgaacg aaagaagaa atatttgtt 
      ctttttgtc catcaaact gtcacaaat gtgtccaag 
      attgacgaa aatcgttac tcttttgcc cacttttct 
      catcaccaa taacactaa caaaaaatt gagatttat 
      ggaacacaa tgagaaaac cgatctctg gatcgactt 
      ggagtcgtc ccaaaccgt gactttctg gcctgaaac 
      ctcctcaat gcaaatgta cgtcaaata cagggacag 
      taaagtgat ttcgcaggc ttttaattt cgcaactcc 
      gaattcaac agtcctccg tatggataa gggaggagc 
      tttgaaagt tgaagttac gggaggcat aagtgactc 
      attccgtgt cacgtttta cgagtataa gtctggcga 
      cgcccccgt tttggtcaa aaactactg tcccgacct 
      atttttgaa gatccagca gcaggacca aagccaggt 
      cactttgct cagccctta caattccct cttgtacac 
      ttgggatga cccaacaag gatcaggaa gctggcttg 
      gaacgttta caacaataa aggagcagg atttgtcct 
      tgacatttg caaggactc acacggaga ggagaaaat 
      tctcttcca cagcgtcac acccaccac tagtgtgac 
      catttcgtc gagcgagga ctcggatag ttccgacag 
      tgttaaaac aacaatgaa taaaaaagt ccgaaggga 
      caaaatcgt gatttactg gctttgcta cgccgacgg 
      gcttcaaag aacgctgct cttcaccca agaatcgcg 
      gatttataa cacggtttt gaaacacta ttttttgga 
      acattgcga cttggagtg tttagtctc gctctgcgg 
      tgtcggagt ttccctttt cacctccga aaatgcgaa 
      aaaagagca cgtttatgg ctcgcttgt tgacagtcc 
      ggccatttg ggaagtgaa cgttggcca taaatggaa 
      aactctgac tttggaatt ggcttcgtg gatcgagga 
      taagtacaa gttgcatta caagaaatg ccagttgcg 
      ggattacat acacatcga ttttgaaaa aatgtacat 
      gttgaagcc tttcttcgc cgaattgaa acgcacgta 
      cacgcctgc ggtttatct ccagtacag gaagtccaa 
      aaattacaa tcatttcaa tttcaaact aaacacact 
      gcagattga taacaaggt caataatac gattgtttt 
      gtacccact tccagggaa tgtcacaaa aggattgaa 
      ctctttcat cgacgtcaa tgttttgaa gtgtttggc 
      gaaagattg cgatattta cagacacat caaagtcaa 
      tattaagga tttctattt gaacatcga aaacgattt 
      gtttgtgga tattcggga acttttggt gcagtataa 
      aaagtttta tgatgagac taatatttc gtacgttaa 
      tgatatcaa gtcaatcca agatttgac tcttgtgcg 
      gtctaaaga tccccaaac tggtcgaag tttgatcga 
      atatttaag tgtgagcag cttcgaaat ccagttaca 
      catctctaa taaaacaca attgatttt aaaaagcag 
      caaactgcc tttgtgcaa aattttgga ctatttgtt 
      tcccataaa catttcgcc ctatatgcc tctggaagg 
      cgtccttca aaaggtttc agctatcga acaaaccaa 
      tctctgcga ccacctctc cacatgtct ggaaaacct 
      actccaaca aataatagc gcaatttct gcaacagta 
      ctattccca cgtcctcgg tttgcaggt tttggtcaa 
      caccttcgc catccaacc tgcgatttt gtacccgca 
      aatcgattt ggaaaaaat gtcaaacac ggttttgta 
      cggctcgag atgtctttt gagtcacat taacatgtc 
      tcccgcggc tttagaagc ttgcgttgt cgatatttt 
      cgctcatca aacgattga aaatattca gaggatccg 
      gagacacgg ctcgtacca aaagtcgga ggtggtacc 
      gaaaatcat aactacgtc ttgtcgcaa cttccacac 
      cggggttcc aaacgcatt gaaccaatt gattgtgag 
      aaaacaaaa cgctgggat taattgtga cggcgaaat 
      gttcttcag aacgaccgg aaaagcgct gttttccct 
      catgacgcg tcaccctga cgagaaaag tgacagtgt 
      cgaagtggc caaagaggc cttgagacg agcctcgat 
      tttagggga accgattcg gattcagca acgtcttac 
      cagatatta caaccgata tttgggcct taaaccgtt 
      ctcagtgta cgaatctat gggacagta tttgctaga 
      aactgttgc taattctag atttatgaa agacaccta 
      ctggtcgca tgcttcgat tgatagaag cggcaaatc 
      agttttgat agtttcgct acagcacgt tgtacgaat 
      aaataagtg ccggctttg gggaccgtg ggatgttga 
      gtgggctat agacttgga tgatatttt atcgatgcc 
      aaaaattac gtctaccga gcttgtctt taatatgag 
      acagagcga aaataatac gagagaatt gttgttgac 
      tatcagcgg tgataaaga aatgctaac ttggatcca 
      cgaaaaaat tgagcaagc tattatagt tagtatgga 
      agataaatc ctggcccgt tgctgaaaa tccataatg 
      ttacatgta ttttctgta aatgctatt agagaaata 
      tt
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