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Gene information
General information
TC000584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3892858..3894173
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000584
    iBeetle screen iB_03045
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000584 RA
      MDQATARRL LSEGAFFIL LGVPEGTEF GIDLKSWNT 
      GEKFRGVKM IPPGVHFIF YNSVSATTD DVAPRVGFF 
      HNFKPGEVL VKKWSKVNE QISDEQVNE SEIVSLKEN 
      LRALDGFLG PYPFDIAER WASLVGSIT ENLLGKLVP 
      QCGQVRAAL ELQSCSDAE RPRGAKRKK GEASEPSCS 
      GIKNLSGCG ELDLLPALK PIEGTELRL TKFPEKNYP 
      EGSSPSEIT QHSLDLTFV FETLLSQYS ESTEIIGEL 
      EFCYICFLV GHSLEAFDQ WKKLVTLFC SCDAAITKY 
      RKLFDLFIT VLEVHIKEI PEEFLADIV SNDNFVYVN 
      LKKLFRSIH ESNVDGQFK SKVVRFKNS LTTTFLWDF 
      EHLDSDEDD EAPVIVET
    CDS sequence
    • >TC000584 RA
      atggaccaa gccacagct cgacgcctg ctctccgag 
      ggcgccttc ttcatcctc ctgggggtc cccgagggc 
      accgagttc ggcatcgac ctcaaatcc tggaacacg 
      ggggagaag ttccgcggg gttaaaatg atcccccca 
      ggcgtgcac ttcatattt tacaactct gttagtgcg 
      acaaccgac gatgtggcc cccagagtt gggtttttc 
      cacaatttt aaaccgggc gaagtcctg gtgaaaaaa 
      tggtcaaag gtaaatgag caaatcagt gacgagcaa 
      gtcaatgag tcggaaatc gtgagtttg aaggagaat 
      ttgagggct cttgatggg tttctgggg ccttatccg 
      ttcgatatt gcggagcgg tgggccagc ttagtgggg 
      agcatcacc gaaaatttg ttggggaaa ttggtgccg 
      cagtgtggg caagtgagg gctgctttg gagctgcag 
      tcgtgcagc gatgccgag cggcccagg ggggctaaa 
      agaaaaaag ggggaggcg agtgagccg tcttgtagc 
      gggataaaa aatttgtcc ggttgtgga gaacttgat 
      ttgttgcct gctttaaaa ccgatcgaa gggacggag 
      ttaaggttg acgaaattc cctgaaaag aactatcct 
      gagggaagc tcgccctct gaaattacg cagcattcc 
      cttgattta acatttgtt tttgaaaca cttttgtca 
      cagtattct gagtctacg gaaatcatc ggtgagctg 
      gaattctgc tacatttgt ttccttgta ggccacagt 
      ttggaggca tttgaccag tggaaaaaa ctagtgact 
      ttgttttgt tcctgtgat gccgctatt acaaaatat 
      agaaaatta ttcgattta tttataaca gttttagaa 
      gtacatata aaggaaata ccggaagaa ttcttagca 
      gatattgtt tcaaacgat aattttgtt tatgttaat 
      ttgaaaaaa ttatttagg tcgattcat gaatcaaac 
      gttgacgga caatttaaa agtaaagtc gttcgattt 
      aaaaattct ttaactacg acgtttttg tgggatttt 
      gaacatctg gattccgat gaagacgac gaagcccca 
      gttattgta gaaacttaa
    mRNA sequence
    • >TC000584 RA
      atcctaacc tcacaattg acaatttgc caaaaacgt 
      aaacaaaca catggacca agccacagc tcgacgcct 
      gctctccga gggcgcctt cttcatcct cctgggggt 
      ccccgaggg caccgagtt cggcatcga cctcaaatc 
      ctggaacac gggggagaa gttccgcgg ggttaaaat 
      gatcccccc aggcgtgca cttcatatt ttacaactc 
      tgttagtgc gacaaccga cgatgtggc ccccagagt 
      tgggttttt ccacaattt taaaccggg cgaagtcct 
      ggtgaaaaa atggtcaaa ggtaaatga gcaaatcag 
      tgacgagca agtcaatga gtcggaaat cgtgagttt 
      gaaggagaa tttgagggc tcttgatgg gtttctggg 
      gccttatcc gttcgatat tgcggagcg gtgggccag 
      cttagtggg gagcatcac cgaaaattt gttggggaa 
      attggtgcc gcagtgtgg gcaagtgag ggctgcttt 
      ggagctgca gtcgtgcag cgatgccga gcggcccag 
      gggggctaa aagaaaaaa gggggaggc gagtgagcc 
      gtcttgtag cgggataaa aaatttgtc cggttgtgg 
      agaacttga tttgttgcc tgctttaaa accgatcga 
      agggacgga gttaaggtt gacgaaatt ccctgaaaa 
      gaactatcc tgagggaag ctcgccctc tgaaattac 
      gcagcattc ccttgattt aacatttgt ttttgaaac 
      acttttgtc acagtattc tgagtctac ggaaatcat 
      cggtgagct ggaattctg ctacatttg tttccttgt 
      aggccacag tttggaggc atttgacca gtggaaaaa 
      actagtgac tttgttttg ttcctgtga tgccgctat 
      tacaaaata tagaaaatt attcgattt atttataac 
      agttttaga agtacatat aaaggaaat accggaaga 
      attcttagc agatattgt ttcaaacga taattttgt 
      ttatgttaa tttgaaaaa attatttag gtcgattca 
      tgaatcaaa cgttgacgg acaatttaa aagtaaagt 
      cgttcgatt taaaaattc tttaactac gacgttttt 
      gtgggattt tgaacatct ggattccga tgaagacga 
      cgaagcccc agttattgt agaaactta attttgttg 
      tgttgaaaa taaaactat tttacttca