Status
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Gene information
General information
TC000565 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9682676..9701806
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000565
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03042

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000565 RA
      MSKFNLDGP KECTSSVCA KTSMELLVK YIDIKADPC 
      EDFYRFSCG NFLENTKLD DRDSRSVTS LLEEDIQTE 
      IKNMLDEPI QTEYPSAFN LAKKFYRAC MNESAIEAE 
      GLKRMKQIF KQIGGWPTL NGNNWRKDS FDWKDAVHK 
      LRQMGINFE YFFILALQR DRIHPDKYV LNIKSPYYQ 
      SQINNEIKN WYLEYMVDV AVLMGAERE RAREEQRQV 
      LEFLLKLAK ISEKNGANQ TQLYNPLSL SELQYEFSE 
      IRWKEYINN ILQPVTEIT YDDIISVSE PLYLKTLLR 
      LLKHTDERI IANFMCWHI LQDLVTYLP QKILSKAYD 
      YVTKATGKP AETSRWNMC VTATENSLS TVISETYIK 
      KYLDDDTRT RAISMIRNI KYQFRENLY KLDWLDAKT 
      KDIASEILV SSVEDIPNY NDFIERNRM YEEVEILED 
      KLLTSALNL DYVSTNIQY GKLRQPVRE NFFETPGSV 
      TGLNIVYSP KENALRIPI PFFRGIFYQ KNRPEYLNY 
      GALGAIIAH EMYHIITQA EYKGLEGEA RSWWSSHTL 
      FNYETKLQC LSNHYGKFI VKMIEHLNA SNTRDEDMA 
      DLAGMKVAY DTYISWVQE NGVEPRLSG LNFSPNQLF 
      WISSAIHHC AKHSDQTLQ RYIANYQLS PGQFRVNGA 
      LQNLDEFAM DFGCPLGAS MNPMQKCRI W
    CDS sequence
    • >TC000565 RA
      atgagcaag tttaattta gatggccct aaagagtgc 
      acatcgtcg gtgtgtgcg aagacttcg atggagttg 
      ctcgtgaag tacatcgac ataaaagcg gatccgtgc 
      gaagatttt tatcggttt tcgtgcggt aatttcctg 
      gagaacaca aagttggat gatcgagac tcgcgctcg 
      gtgactagt ctcctcgaa gaggatatt caaaccgaa 
      attaaaaac atgctggat gaaccgatc cagaccgaa 
      tatccctca gctttcaat ttagcaaag aaattttat 
      cgagcttgc atgaacgag agtgcaatc gaagcagaa 
      ggattaaaa aggatgaaa caaattttt aagcaaata 
      ggcggatgg cccactctt aacgggaat aattggcgc 
      aaggattca ttcgattgg aaagatgcc gtgcacaaa 
      ctcaggcag atgggaatt aatttcgaa tattttttc 
      attttggcg ctgcaaagg gatcgaata catccagat 
      aaatacgtt ctaaatata aagagcccg tactatcaa 
      agtcaaata aataacgaa ataaaaaac tggtattta 
      gaatatatg gtggacgtc gctgtactc atgggagcc 
      gagagggag agagcgaga gaagagcaa cgccaagtc 
      ttggagttt ctgctcaaa ttagctaaa atttctgaa 
      aagaacgga gctaatcaa actcaactt tataatcct 
      ttatccttg tcggaatta caatatgaa ttttcagaa 
      atacgttgg aaggaatat attaacaat attctacag 
      cccgtcact gaaattact tatgacgat attatatcc 
      gtctcggag cctctttat ttaaaaacg ttgcttcgt 
      ctccttaaa cacaccgat gaaaggata atcgccaat 
      ttcatgtgc tggcatatt ttacaagac ttggtcact 
      tacttaccc caaaaaatc ttaagtaaa gcgtacgat 
      tatgtcacg aaagctact ggaaaaccg gcagaaacg 
      tcacggtgg aatatgtgc gttacagcc accgaaaat 
      agcctctcg actgttatt tccgaaact tatatcaag 
      aaatattta gatgacgac acacgaacg cgtgctatt 
      tctatgatt cgaaatatc aaatatcag ttcagggaa 
      aatctttac aaattagat tggctggat gcaaagacc 
      aaagacatt gcctcagaa atactcgtt tcgagcgta 
      gaagacatt ccaaattac aatgacttt attgaaaga 
      aacagaatg tacgaagag gtggaaata ttggaagat 
      aagctcttg acgtcggcc ctaaatctg gattatgtg 
      agtaccaat attcaatat ggaaaactc cggcaacct 
      gtaagagag aattttttc gaaaccccc ggatctgtc 
      acaggatta aatattgtt tactcgcca aaggaaaat 
      gctctgaga ataccaatc cccttcttc cggggcatt 
      ttctaccag aaaaaccgg cccgagtac ctcaactac 
      ggcgccttg ggcgcgatt atcgcccat gagatgtac 
      cacatcatc acccaggcc gagtacaaa ggactcgag 
      ggcgaagcc cggagctgg tggtcatcc cacaccctc 
      ttcaactac gaaactaaa ctgcagtgt ctgtcgaat 
      cattacgga aaattcata gttaaaatg atcgaacac 
      ttgaacgct tcgaacact cgcgacgaa gacatggcc 
      gatttggcc ggaatgaaa gttgcctac gacacttac 
      atcagctgg gttcaagaa aacggagtc gaaccacga 
      ctttccgga ttaaacttc tcgccgaac caattgttc 
      tggatatca tcggcgatc catcactgc gccaagcac 
      tccgatcaa actttacag agatacatc gctaattat 
      cagctgagc cccggacag ttccgcgtc aacggggct 
      ctgcaaaat ttagatgag ttcgcaatg gatttcgga 
      tgtccactc ggagccagc atgaatccc atgcaaaag 
      tgccggatt tggtag
    mRNA sequence
    • >TC000565 RA
      gcgataaaa cgttcaagt ccaatattt acaataaag 
      ccgctttcc agccatgag caagtttaa tttagatgg 
      ccctaaaga gtgcacatc gtcggtgtg tgcgaagac 
      ttcgatgga gttgctcgt gaagtacat cgacataaa 
      agcggatcc gtgcgaaga tttttatcg gttttcgtg 
      cggtaattt cctggagaa cacaaagtt ggatgatcg 
      agactcgcg ctcggtgac tagtctcct cgaagagga 
      tattcaaac cgaaattaa aaacatgct ggatgaacc 
      gatccagac cgaatatcc ctcagcttt caatttagc 
      aaagaaatt ttatcgagc ttgcatgaa cgagagtgc 
      aatcgaagc agaaggatt aaaaaggat gaaacaaat 
      ttttaagca aataggcgg atggcccac tcttaacgg 
      gaataattg gcgcaagga ttcattcga ttggaaaga 
      tgccgtgca caaactcag gcagatggg aattaattt 
      cgaatattt tttcatttt ggcgctgca aagggatcg 
      aatacatcc agataaata cgttctaaa tataaagag 
      cccgtacta tcaaagtca aataaataa cgaaataaa 
      aaactggta tttagaata tatggtgga cgtcgctgt 
      actcatggg agccgagag ggagagagc gagagaaga 
      gcaacgcca agtcttgga gtttctgct caaattagc 
      taaaatttc tgaaaagaa cggagctaa tcaaactca 
      actttataa tcctttatc cttgtcgga attacaata 
      tgaattttc agaaatacg ttggaagga atatattaa 
      caatattct acagcccgt cactgaaat tacttatga 
      cgatattat atccgtctc ggagcctct ttatttaaa 
      aacgttgct tcgtctcct taaacacac cgatgaaag 
      gataatcgc caatttcat gtgctggca tattttaca 
      agacttggt cacttactt accccaaaa aatcttaag 
      taaagcgta cgattatgt cacgaaagc tactggaaa 
      accggcaga aacgtcacg gtggaatat gtgcgttac 
      agccaccga aaatagcct ctcgactgt tatttccga 
      aacttatat caagaaata tttagatga cgacacacg 
      aacgcgtgc tatttctat gattcgaaa tatcaaata 
      tcagttcag ggaaaatct ttacaaatt agattggct 
      ggatgcaaa gaccaaaga cattgcctc agaaatact 
      cgtttcgag cgtagaaga cattccaaa ttacaatga 
      ctttattga aagaaacag aatgtacga agaggtgga 
      aatattgga agataagct cttgacgtc ggccctaaa 
      tctggatta tgtgagtac caatattca atatggaaa 
      actccggca acctgtaag agagaattt tttcgaaac 
      ccccggatc tgtcacagg attaaatat tgtttactc 
      gccaaagga aaatgctct gagaatacc aatcccctt 
      cttccgggg cattttcta ccagaaaaa ccggcccga 
      gtacctcaa ctacggcgc cttgggcgc gattatcgc 
      ccatgagat gtaccacat catcaccca ggccgagta 
      caaaggact cgagggcga agcccggag ctggtggtc 
      atcccacac cctcttcaa ctacgaaac taaactgca 
      gtgtctgtc gaatcatta cggaaaatt catagttaa 
      aatgatcga acacttgaa cgcttcgaa cactcgcga 
      cgaagacat ggccgattt ggccggaat gaaagttgc 
      ctacgacac ttacatcag ctgggttca agaaaacgg 
      agtcgaacc acgactttc cggattaaa cttctcgcc 
      gaaccaatt gttctggat atcatcggc gatccatca 
      ctgcgccaa gcactccga tcaaacttt acagagata 
      catcgctaa ttatcagct gagccccgg acagttccg 
      cgtcaacgg ggctctgca aaatttaga tgagttcgc 
      aatggattt cggatgtcc actcggagc cagcatgaa 
      tcccatgca aaagtgccg gatttggta gattcatct 
      tttaagact ggactaata ggggcgcaa actggccgt 
      agccgataa tgtgccgta ttgattatt gtatcactg 
      cgttacttt ttacttttc tcgtccacg gctcgtatc 
      tctttccac accacttaa agcattata ttacacgct 
      gaataaatc atccaaact tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY