General information | |||
TC000541 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10007207..10009128 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC000541 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03036 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
head capsule decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head bristle irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: The irregular head bristle pattern is potentially due to the decreased head capsule (secondary defect). |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000541 RA
MSRDESFVA VKNTAESMV PSIGSDDED SFVVLETSN SISESLSCS PPRDEILPP IRTNKVHEM ENLIRMKIS QITVDPLPE LPAAVDAIV PSVASIAIS PDSSYEEIQ KQVEELIKE NEKLKNIIA QNNMSMKAQ YTRFLAYRD EVTNVMKSY KDKFNDAKM CIDKYKEEN AKLVSDVEV YINLKKLHE EEILNLKLK LKELENLTN GPNFSVVDT RKLAMMKDL ELKQEEIER LTQKLVNVP NLEQELQTK QQQIDDFKL DILAAKNRE SDLIDEIQS MKTTTTDLQ SKLDNLTKE NAKLVQSST AAQTYDQLL KLKETEILK LKEEQQRTA FVESTLSDE IKTLKSQLK ELQDKYNPR LAEDYAQSR AQLSVAQRT ITALEQERE QKIKEIEDL RKLLQENTS ANDEMFALK EQIEVYKND FEAEKKSKL AMKQEKDQV VEDLQNLQT RNKQLQNEV ERLRARNEP PRAEGYQCP KCSFGFNNY QSLENHVHR CLDLDGFP
- >TC000541 RA
atgagtcgt gacgagtcc ttcgttgct gtgaaaaac actgcagag tccatggtt ccgtcaatc gggtccgat gatgaagat tcatttgtc gttttggaa acttcaaat tccatcagc gaaagtttg tcttgcagc cctccaagg gacgaaatc ttgccccca ataaggaca aataaggtt cacgaaatg gaaaatcta atcaggatg aaaatatca caaattacc gttgatccg ttgccagaa ttaccagca gctgttgat gcgattgtg ccctcagtt gcaagtatt gcaatatcg ccggattcg tcgtatgaa gagattcaa aaacaagtg gaagaactg attaaggaa aacgaaaag ttgaagaat atcatcgca caaaataac atgagtatg aaggcgcag tatacgagg tttttggcc tacagggat gaggtcacc aatgtgatg aagtcctac aaagataag ttcaacgat gccaaaatg tgcatagat aagtataaa gaagaaaat gcaaagttg gttagtgac gttgaagtt tacatcaat cttaaaaag ctccatgaa gaagaaatc ttgaatttg aaactgaag cttaaggaa ttggaaaat ttgaccaac ggccccaac ttttcagtt gttgacaca aggaaatta gcaatgatg aaggattta gaactgaaa caggaagaa attgaaaga ctgacgcaa aaattggtg aatgtaccg aaccttgaa caggaatta caaaccaag cagcaacaa attgacgac tttaaactg gatattctc gccgcaaaa aatcgcgaa agcgaccta attgacgaa atccagtca atgaagaca acaaccacc gacctccaa tcaaaattg gataattta acaaaagaa aacgcaaaa ctcgtccaa tcatcgacg gcagcccaa acctacgac cagctcctc aaactgaaa gaaacggaa atcttgaaa ctgaaggaa gaacaacaa cgcaccgct ttcgtcgaa tccaccctc agtgatgag ataaaaacc ctaaaatcg caacttaag gagctccaa gacaagtac aacccgcgt ttggccgaa gattatgcc caatcccgg gcccagttg agcgttgcc caaaggaca atcacagca ttggaacag gaaagggaa cagaagatt aaagaaatc gaagatttg cgtaaacta cttcaagaa aatacaagt gccaacgac gaaatgttc gcattgaag gaacaaatc gaagtgtat aagaacgat tttgaagca gagaagaag agcaaactt gcgatgaaa caagagaag gaccaggtt gttgaagat ttgcagaat ctgcaaacg aggaacaaa caactgcag aatgaagtg gagaggctg agggcgagg aacgagccg ccgagggcc gaggggtat cagtgcccc aagtgctcg ttcggattt aataattac cagtcattg gaaaatcat gttcatagg tgcttggat ttggacggt tttccctaa
- >TC000541 RA
ctacagttg tgtcccaaa tgaaatttt acgtaaata gttatgcca caataacat ttttagagc tttaatatt cttaaaaaa ttaaattaa ggctaaggc gacttttta ttggtccgg ttggtagca gtgtcttta atcgtgtgt cagtagttt ggtattttc atattctat gttctaatt cattctatt ttgcgccgc aaatgtagt gttgtactg cagctgttg ttaaaactt gaaagtcat caaaaatga gtcgtgacg agtccttcg ttgctgtga aaaacactg cagagtcca tggttccgt caatcgggt ccgatgatg aagattcat ttgtcgttt tggaaactt caaattcca tcagcgaaa gtttgtctt gcagccctc caagggacg aaatcttgc ccccaataa ggacaaata aggttcacg aaatggaaa atctaatca ggatgaaaa tatcacaaa ttaccgttg atccgttgc cagaattac cagcagctg ttgatgcga ttgtgccct cagttgcaa gtattgcaa tatcgccgg attcgtcgt atgaagaga ttcaaaaac aagtggaag aactgatta aggaaaacg aaaagttga agaatatca tcgcacaaa ataacatga gtatgaagg cgcagtata cgaggtttt tggcctaca gggatgagg tcaccaatg tgatgaagt cctacaaag ataagttca acgatgcca aaatgtgca tagataagt ataaagaag aaaatgcaa agttggtta gtgacgttg aagtttaca tcaatctta aaaagctcc atgaagaag aaatcttga atttgaaac tgaagctta aggaattgg aaaatttga ccaacggcc ccaactttt cagttgttg acacaagga aattagcaa tgatgaagg atttagaac tgaaacagg aagaaattg aaagactga cgcaaaaat tggtgaatg taccgaacc ttgaacagg aattacaaa ccaagcagc aacaaattg acgacttta aactggata ttctcgccg caaaaaatc gcgaaagcg acctaattg acgaaatcc agtcaatga agacaacaa ccaccgacc tccaatcaa aattggata atttaacaa aagaaaacg caaaactcg tccaatcat cgacggcag cccaaacct acgaccagc tcctcaaac tgaaagaaa cggaaatct tgaaactga aggaagaac aacaacgca ccgctttcg tcgaatcca ccctcagtg atgagataa aaaccctaa aatcgcaac ttaaggagc tccaagaca agtacaacc cgcgtttgg ccgaagatt atgcccaat cccgggccc agttgagcg ttgcccaaa ggacaatca cagcattgg aacaggaaa gggaacaga agattaaag aaatcgaag atttgcgta aactacttc aagaaaata caagtgcca acgacgaaa tgttcgcat tgaaggaac aaatcgaag tgtataaga acgattttg aagcagaga agaagagca aacttgcga tgaaacaag agaaggacc aggttgttg aagatttgc agaatctgc aaacgagga acaaacaac tgcagaatg aagtggaga ggctgaggg cgaggaacg agccgccga gggccgagg ggtatcagt gccccaagt gctcgttcg gatttaata attaccagt cattggaaa atcatgttc ataggtgct tggatttgg acggttttc cctaaatat attttttga ctagtattt gtttattgt tcttatata aatatctaa aggaattaa taagtttaa caaggccat cgtaattgt ttggaaatg tattatttt gtagttact gttcctcgt ttacaacaa taaatacga gttaataaa gttcttatt tgtct
