Status
Please wait ...
Gene information
General information
TC030996
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000536,TC000535 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4606463..4609680
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030996
    iBeetle screen iB_03035
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09647
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030996 RA
      MAQNNIKVL LGFEDELIT EKYKNQVNF 
TTNKIGGKP DLPSNIKLE PPICPLCQL 
PRPLVVQVY APLESSPYH RTLYLFACI 
NPNCWNQSE SWICIRVQS QEKLIEHEE 
PSAAVTSKT SVTDWCADA DDWDDNNAN 
MNEENGNLI NNIERVSDE DDESCSFEE 
SVRTGLGNL TVDDRNANN GAFGKCLF
    • >TC030996 RB
      MAQNNIKVL LGFEDELIT EKYKNQVNF TTNKIGGKP 
DLPSNIKLE PPICPLCQL PRPLVVQVY APLESSPYH 
RTLYLFACI NPNCWNQSE SWICIRVQS QEKLIEHEE 
PSAAVTSKT SVTDWCADA DDWDDNNAN MNEENGNLI 
NNIERVSDE DDESCSFEE SVRTGLGNL TVDDRNANN 
GAFGGAVGR LHSPSATAE IEGDEGEVV TIDSPVMPQ 
QDIVALLQE ATPLPQLHG DPRKSSLFQ FISSFMGVW 
EETAGASTI SDRHVKELL QEYQQKNED DMNLVSPDT 
GGASAQIAD NVYEKYEKS NPAHGDKMF HHFLSKIQM 
NPSQILRYN RDSAPLLLY PLQGLQTTC NYCKGDLVF 
EFQVLPTII PKLKLVGDA KHCSRLEFG TVLVFTCRK 
SCWSTDTNT RHETVIVQS EVY
    • >TC030996 RC
      MPQQDIVAL LQEATPLPQ LHGDPRKSS LFQFISSFM 
GVWEETAGA STISDRHVK ELLQEYQQK NEDDMNLVS 
PDTGGASAQ IADNVYEKY EKSNPAHGD KMFHHFLSK 
IQMNPSQIL RYNRDSAPL LLYPLQGLQ TTCNYCKGD 
LVFEFQVLP TIIPKLKLV GDAKHCSRL EFGTVLVFT 
CRKSCWSTD TNTRHETVI VQSEVY
    CDS sequence
    • >TC030996 RA
      atggcccaa aataatatt aaagtactg ctcggtttt 
gaagacgaa ctcattact gagaaatat aaaaaccaa 
gttaacttc accaccaat aaaattgga ggaaaacct 
gatttacca agcaatatc aagcttgag ccgccaatc 
tgtcccctg tgccaacta ccaaggcct ttggtagtc 
caggtctat gcacctctg gagtcctcc ccataccac 
cgaacttta tacctattc gcttgcata aatccaaat 
tgttggaat caaagcgaa agctggatt tgtattcgg 
gtccagtcg caggaaaaa ctaattgaa catgaagaa 
ccatcagct gctgtaact tccaaaact agtgtaact 
gactggtgt gcagatgct gatgattgg gatgataac 
aacgccaac atgaacgaa gaaaacggc aatttaata 
aataacatt gaacgagtt tcggacgaa gatgacgaa 
agttgcagt tttgaagag tcagtgagg acgggactt 
gggaattta acagtggac gatagaaat gcaaataat 
ggagcgttt ggtaagtgt ttattttga
    • >TC030996 RB
      atggcccaa aataatatt aaagtactg ctcggtttt 
gaagacgaa ctcattact gagaaatat aaaaaccaa 
gttaacttc accaccaat aaaattgga ggaaaacct 
gatttacca agcaatatc aagcttgag ccgccaatc 
tgtcccctg tgccaacta ccaaggcct ttggtagtc 
caggtctat gcacctctg gagtcctcc ccataccac 
cgaacttta tacctattc gcttgcata aatccaaat 
tgttggaat caaagcgaa agctggatt tgtattcgg 
gtccagtcg caggaaaaa ctaattgaa catgaagaa 
ccatcagct gctgtaact tccaaaact agtgtaact 
gactggtgt gcagatgct gatgattgg gatgataac 
aacgccaac atgaacgaa gaaaacggc aatttaata 
aataacatt gaacgagtt tcggacgaa gatgacgaa 
agttgcagt tttgaagag tcagtgagg acgggactt 
gggaattta acagtggac gatagaaat gcaaataat 
ggagcgttt ggtggtgcc gtaggccgg ctgcactcg 
ccctcggcc accgcggaa atcgagggc gacgaaggc 
gaagttgtg accatcgat agccccgtt atgccccaa 
caagacatc gttgccctt ctgcaggaa gccacccct 
ttaccgcag ttgcacggg gacccccgc aaatcctcc 
ctttttcaa ttcatttca tcgttcatg ggcgtgtgg 
gaggaaacc gctggtgcg tccaccatc agcgataga 
cacgttaaa gaactgtta caagaatat caacagaaa 
aacgaagac gatatgaat ttagtgagt cctgacact 
ggaggggcg tccgcacaa atcgccgat aacgtgtac 
gaaaagtac gaaaaaagc aatccagct catggcgat 
aaaatgttt caccacttt ctctcaaag attcagatg 
aatcctagt cagattttg aggtataat agggacagc 
gccccactg ctcttgtac cctttacaa gggcttcaa 
acgacgtgc aattactgt aaaggcgat ttagttttc 
gaatttcaa gttttgcca acgattatt ccaaagctg 
aaattggtg ggcgatgcc aaacattgt tcaagactg 
gagttcggg actgtttta gtatttaca tgtcgcaag 
agttgctgg tcaaccgat acgaatacg agacatgag 
actgtcatt gtacaatca gaagtttac tga
    • >TC030996 RC
      atgccccaa caagacatc gttgccctt ctgcaggaa 
gccacccct ttaccgcag ttgcacggg gacccccgc 
aaatcctcc ctttttcaa ttcatttca tcgttcatg 
ggcgtgtgg gaggaaacc gctggtgcg tccaccatc 
agcgataga cacgttaaa gaactgtta caagaatat 
caacagaaa aacgaagac gatatgaat ttagtgagt 
cctgacact ggaggggcg tccgcacaa atcgccgat 
aacgtgtac gaaaagtac gaaaaaagc aatccagct 
catggcgat aaaatgttt caccacttt ctctcaaag 
attcagatg aatcctagt cagattttg aggtataat 
agggacagc gccccactg ctcttgtac cctttacaa 
gggcttcaa acgacgtgc aattactgt aaaggcgat 
ttagttttc gaatttcaa gttttgcca acgattatt 
ccaaagctg aaattggtg ggcgatgcc aaacattgt 
tcaagactg gagttcggg actgtttta gtatttaca 
tgtcgcaag agttgctgg tcaaccgat acgaatacg 
agacatgag actgtcatt gtacaatca gaagtttac 
tga
    mRNA sequence
    • >TC030996 RA
      tacgagtag tgaataagt tttgttgaa taaatttat 
cagctgatg ctgaaacat ggcccaaaa taatattaa 
agtactgct cggttttga agacgaact cattactga 
gaaatataa aaaccaagt taacttcac caccaataa 
aattggagg aaaacctga tttaccaag caatatcaa 
gcttgagcc gccaatctg tcccctgtg ccaactacc 
aaggccttt ggtagtcca ggtctatgc acctctgga 
gtcctcccc ataccaccg aactttata cctattcgc 
ttgcataaa tccaaattg ttggaatca aagcgaaag 
ctggatttg tattcgggt ccagtcgca ggaaaaact 
aattgaaca tgaagaacc atcagctgc tgtaacttc 
caaaactag tgtaactga ctggtgtgc agatgctga 
tgattggga tgataacaa cgccaacat gaacgaaga 
aaacggcaa tttaataaa taacattga acgagtttc 
ggacgaaga tgacgaaag ttgcagttt tgaagagtc 
agtgaggac gggacttgg gaatttaac agtggacga 
tagaaatgc aaataatgg agcgtttgg taagtgttt 
attttgatg caacggtat atacaataa ataaaatta 
catcacaaa t
    • >TC030996 RB
      tacgagtag tgaataagt tttgttgaa taaatttat 
cagctgatg ctgaaacat ggcccaaaa taatattaa 
agtactgct cggttttga agacgaact cattactga 
gaaatataa aaaccaagt taacttcac caccaataa 
aattggagg aaaacctga tttaccaag caatatcaa 
gcttgagcc gccaatctg tcccctgtg ccaactacc 
aaggccttt ggtagtcca ggtctatgc acctctgga 
gtcctcccc ataccaccg aactttata cctattcgc 
ttgcataaa tccaaattg ttggaatca aagcgaaag 
ctggatttg tattcgggt ccagtcgca ggaaaaact 
aattgaaca tgaagaacc atcagctgc tgtaacttc 
caaaactag tgtaactga ctggtgtgc agatgctga 
tgattggga tgataacaa cgccaacat gaacgaaga 
aaacggcaa tttaataaa taacattga acgagtttc 
ggacgaaga tgacgaaag ttgcagttt tgaagagtc 
agtgaggac gggacttgg gaatttaac agtggacga 
tagaaatgc aaataatgg agcgtttgg tggtgccgt 
aggccggct gcactcgcc ctcggccac cgcggaaat 
cgagggcga cgaaggcga agttgtgac catcgatag 
ccccgttat gccccaaca agacatcgt tgcccttct 
gcaggaagc caccccttt accgcagtt gcacgggga 
cccccgcaa atcctccct ttttcaatt catttcatc 
gttcatggg cgtgtggga ggaaaccgc tggtgcgtc 
caccatcag cgatagaca cgttaaaga actgttaca 
agaatatca acagaaaaa cgaagacga tatgaattt 
agtgagtcc tgacactgg aggggcgtc cgcacaaat 
cgccgataa cgtgtacga aaagtacga aaaaagcaa 
tccagctca tggcgataa aatgtttca ccactttct 
ctcaaagat tcagatgaa tcctagtca gattttgag 
gtataatag ggacagcgc cccactgct cttgtaccc 
tttacaagg gcttcaaac gacgtgcaa ttactgtaa 
aggcgattt agttttcga atttcaagt tttgccaac 
gattattcc aaagctgaa attggtggg cgatgccaa 
acattgttc aagactgga gttcgggac tgttttagt 
atttacatg tcgcaagag ttgctggtc aaccgatac 
gaatacgag acatgagac tgtcattgt acaatcaga 
agtttactg aatattatg caatatcgc ggcgaaaaa 
attccacca ttgcttgcc actctttgt ttgttgtaa 
tttcgtgtt tattacatc acaaagcga ttagggcgg 
gtcatactt ttcttccaa aaatagaga atgtaacgt 
aattattgt agtctcagg aattgtgca attcgcaag 
ttttttgcc ttcataaaa atattacag ttggtgtta 
cggtaattt tatgctctt taacgtagg cgctccgaa 
actattttc tgagt
    • >TC030996 RC
      ccggggagg ctttttttt ttaattttt ttgatttcg 
aaattattt caggtggtg ccgtaggcc ggctgcact 
cgccctcgg ccaccgcgg aaatcgagg gcgacgaag 
gcgaagttg tgaccatcg atagccccg ttatgcccc 
aacaagaca tcgttgccc ttctgcagg aagccaccc 
ctttaccgc agttgcacg gggaccccc gcaaatcct 
ccctttttc aattcattt catcgttca tgggcgtgt 
gggaggaaa ccgctggtg cgtccacca tcagcgata 
gacacgtta aagaactgt tacaagaat atcaacaga 
aaaacgaag acgatatga atttagtga gtcctgaca 
ctggagggg cgtccgcac aaatcgccg ataacgtgt 
acgaaaagt acgaaaaaa gcaatccag ctcatggcg 
ataaaatgt ttcaccact ttctctcaa agattcaga 
tgaatccta gtcagattt tgaggtata atagggaca 
gcgccccac tgctcttgt accctttac aagggcttc 
aaacgacgt gcaattact gtaaaggcg atttagttt 
tcgaatttc aagttttgc caacgatta ttccaaagc 
tgaaattgg tgggcgatg ccaaacatt gttcaagac 
tggagttcg ggactgttt tagtattta catgtcgca 
agagttgct ggtcaaccg atacgaata cgagacatg 
agactgtca ttgtacaat cagaagttt actgaatat 
tatgcaata tcgcggcga aaaaattcc accattgct 
tgccactct ttgtttgtt gtaatttcg tgtttatta 
catcacaaa gcgattagg gcgggtcat acttttctt 
ccaaaaata gagaatgta acgtaatta ttgtagtct 
caggaattg tgcaattcg caagttttt tgccttcat 
aaaaatatt acagttggt gttacggta attttatgc 
tctttaacg taggcgctc cgaaactat tttctgagt