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Gene information
General information
TC030996
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000536,TC000535
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4606463..4609680
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030996
    iBeetle screen iB_03035
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09647
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030996 RA
      MAQNNIKVL LGFEDELIT EKYKNQVNF 
      TTNKIGGKP DLPSNIKLE PPICPLCQL 
      PRPLVVQVY APLESSPYH RTLYLFACI 
      NPNCWNQSE SWICIRVQS QEKLIEHEE 
      PSAAVTSKT SVTDWCADA DDWDDNNAN 
      MNEENGNLI NNIERVSDE DDESCSFEE 
      SVRTGLGNL TVDDRNANN GAFGKCLF
    • >TC030996 RB
      MAQNNIKVL LGFEDELIT EKYKNQVNF TTNKIGGKP 
      DLPSNIKLE PPICPLCQL PRPLVVQVY APLESSPYH 
      RTLYLFACI NPNCWNQSE SWICIRVQS QEKLIEHEE 
      PSAAVTSKT SVTDWCADA DDWDDNNAN MNEENGNLI 
      NNIERVSDE DDESCSFEE SVRTGLGNL TVDDRNANN 
      GAFGGAVGR LHSPSATAE IEGDEGEVV TIDSPVMPQ 
      QDIVALLQE ATPLPQLHG DPRKSSLFQ FISSFMGVW 
      EETAGASTI SDRHVKELL QEYQQKNED DMNLVSPDT 
      GGASAQIAD NVYEKYEKS NPAHGDKMF HHFLSKIQM 
      NPSQILRYN RDSAPLLLY PLQGLQTTC NYCKGDLVF 
      EFQVLPTII PKLKLVGDA KHCSRLEFG TVLVFTCRK 
      SCWSTDTNT RHETVIVQS EVY
    • >TC030996 RC
      MPQQDIVAL LQEATPLPQ LHGDPRKSS LFQFISSFM 
      GVWEETAGA STISDRHVK ELLQEYQQK NEDDMNLVS 
      PDTGGASAQ IADNVYEKY EKSNPAHGD KMFHHFLSK 
      IQMNPSQIL RYNRDSAPL LLYPLQGLQ TTCNYCKGD 
      LVFEFQVLP TIIPKLKLV GDAKHCSRL EFGTVLVFT 
      CRKSCWSTD TNTRHETVI VQSEVY
    CDS sequence
    • >TC030996 RA
      atggcccaa aataatatt aaagtactg ctcggtttt 
      gaagacgaa ctcattact gagaaatat aaaaaccaa 
      gttaacttc accaccaat aaaattgga ggaaaacct 
      gatttacca agcaatatc aagcttgag ccgccaatc 
      tgtcccctg tgccaacta ccaaggcct ttggtagtc 
      caggtctat gcacctctg gagtcctcc ccataccac 
      cgaacttta tacctattc gcttgcata aatccaaat 
      tgttggaat caaagcgaa agctggatt tgtattcgg 
      gtccagtcg caggaaaaa ctaattgaa catgaagaa 
      ccatcagct gctgtaact tccaaaact agtgtaact 
      gactggtgt gcagatgct gatgattgg gatgataac 
      aacgccaac atgaacgaa gaaaacggc aatttaata 
      aataacatt gaacgagtt tcggacgaa gatgacgaa 
      agttgcagt tttgaagag tcagtgagg acgggactt 
      gggaattta acagtggac gatagaaat gcaaataat 
      ggagcgttt ggtaagtgt ttattttga
    • >TC030996 RB
      atggcccaa aataatatt aaagtactg ctcggtttt 
      gaagacgaa ctcattact gagaaatat aaaaaccaa 
      gttaacttc accaccaat aaaattgga ggaaaacct 
      gatttacca agcaatatc aagcttgag ccgccaatc 
      tgtcccctg tgccaacta ccaaggcct ttggtagtc 
      caggtctat gcacctctg gagtcctcc ccataccac 
      cgaacttta tacctattc gcttgcata aatccaaat 
      tgttggaat caaagcgaa agctggatt tgtattcgg 
      gtccagtcg caggaaaaa ctaattgaa catgaagaa 
      ccatcagct gctgtaact tccaaaact agtgtaact 
      gactggtgt gcagatgct gatgattgg gatgataac 
      aacgccaac atgaacgaa gaaaacggc aatttaata 
      aataacatt gaacgagtt tcggacgaa gatgacgaa 
      agttgcagt tttgaagag tcagtgagg acgggactt 
      gggaattta acagtggac gatagaaat gcaaataat 
      ggagcgttt ggtggtgcc gtaggccgg ctgcactcg 
      ccctcggcc accgcggaa atcgagggc gacgaaggc 
      gaagttgtg accatcgat agccccgtt atgccccaa 
      caagacatc gttgccctt ctgcaggaa gccacccct 
      ttaccgcag ttgcacggg gacccccgc aaatcctcc 
      ctttttcaa ttcatttca tcgttcatg ggcgtgtgg 
      gaggaaacc gctggtgcg tccaccatc agcgataga 
      cacgttaaa gaactgtta caagaatat caacagaaa 
      aacgaagac gatatgaat ttagtgagt cctgacact 
      ggaggggcg tccgcacaa atcgccgat aacgtgtac 
      gaaaagtac gaaaaaagc aatccagct catggcgat 
      aaaatgttt caccacttt ctctcaaag attcagatg 
      aatcctagt cagattttg aggtataat agggacagc 
      gccccactg ctcttgtac cctttacaa gggcttcaa 
      acgacgtgc aattactgt aaaggcgat ttagttttc 
      gaatttcaa gttttgcca acgattatt ccaaagctg 
      aaattggtg ggcgatgcc aaacattgt tcaagactg 
      gagttcggg actgtttta gtatttaca tgtcgcaag 
      agttgctgg tcaaccgat acgaatacg agacatgag 
      actgtcatt gtacaatca gaagtttac tga
    • >TC030996 RC
      atgccccaa caagacatc gttgccctt ctgcaggaa 
      gccacccct ttaccgcag ttgcacggg gacccccgc 
      aaatcctcc ctttttcaa ttcatttca tcgttcatg 
      ggcgtgtgg gaggaaacc gctggtgcg tccaccatc 
      agcgataga cacgttaaa gaactgtta caagaatat 
      caacagaaa aacgaagac gatatgaat ttagtgagt 
      cctgacact ggaggggcg tccgcacaa atcgccgat 
      aacgtgtac gaaaagtac gaaaaaagc aatccagct 
      catggcgat aaaatgttt caccacttt ctctcaaag 
      attcagatg aatcctagt cagattttg aggtataat 
      agggacagc gccccactg ctcttgtac cctttacaa 
      gggcttcaa acgacgtgc aattactgt aaaggcgat 
      ttagttttc gaatttcaa gttttgcca acgattatt 
      ccaaagctg aaattggtg ggcgatgcc aaacattgt 
      tcaagactg gagttcggg actgtttta gtatttaca 
      tgtcgcaag agttgctgg tcaaccgat acgaatacg 
      agacatgag actgtcatt gtacaatca gaagtttac 
      tga
    mRNA sequence
    • >TC030996 RA
      tacgagtag tgaataagt tttgttgaa taaatttat 
      cagctgatg ctgaaacat ggcccaaaa taatattaa 
      agtactgct cggttttga agacgaact cattactga 
      gaaatataa aaaccaagt taacttcac caccaataa 
      aattggagg aaaacctga tttaccaag caatatcaa 
      gcttgagcc gccaatctg tcccctgtg ccaactacc 
      aaggccttt ggtagtcca ggtctatgc acctctgga 
      gtcctcccc ataccaccg aactttata cctattcgc 
      ttgcataaa tccaaattg ttggaatca aagcgaaag 
      ctggatttg tattcgggt ccagtcgca ggaaaaact 
      aattgaaca tgaagaacc atcagctgc tgtaacttc 
      caaaactag tgtaactga ctggtgtgc agatgctga 
      tgattggga tgataacaa cgccaacat gaacgaaga 
      aaacggcaa tttaataaa taacattga acgagtttc 
      ggacgaaga tgacgaaag ttgcagttt tgaagagtc 
      agtgaggac gggacttgg gaatttaac agtggacga 
      tagaaatgc aaataatgg agcgtttgg taagtgttt 
      attttgatg caacggtat atacaataa ataaaatta 
      catcacaaa t
    • >TC030996 RB
      tacgagtag tgaataagt tttgttgaa taaatttat 
      cagctgatg ctgaaacat ggcccaaaa taatattaa 
      agtactgct cggttttga agacgaact cattactga 
      gaaatataa aaaccaagt taacttcac caccaataa 
      aattggagg aaaacctga tttaccaag caatatcaa 
      gcttgagcc gccaatctg tcccctgtg ccaactacc 
      aaggccttt ggtagtcca ggtctatgc acctctgga 
      gtcctcccc ataccaccg aactttata cctattcgc 
      ttgcataaa tccaaattg ttggaatca aagcgaaag 
      ctggatttg tattcgggt ccagtcgca ggaaaaact 
      aattgaaca tgaagaacc atcagctgc tgtaacttc 
      caaaactag tgtaactga ctggtgtgc agatgctga 
      tgattggga tgataacaa cgccaacat gaacgaaga 
      aaacggcaa tttaataaa taacattga acgagtttc 
      ggacgaaga tgacgaaag ttgcagttt tgaagagtc 
      agtgaggac gggacttgg gaatttaac agtggacga 
      tagaaatgc aaataatgg agcgtttgg tggtgccgt 
      aggccggct gcactcgcc ctcggccac cgcggaaat 
      cgagggcga cgaaggcga agttgtgac catcgatag 
      ccccgttat gccccaaca agacatcgt tgcccttct 
      gcaggaagc caccccttt accgcagtt gcacgggga 
      cccccgcaa atcctccct ttttcaatt catttcatc 
      gttcatggg cgtgtggga ggaaaccgc tggtgcgtc 
      caccatcag cgatagaca cgttaaaga actgttaca 
      agaatatca acagaaaaa cgaagacga tatgaattt 
      agtgagtcc tgacactgg aggggcgtc cgcacaaat 
      cgccgataa cgtgtacga aaagtacga aaaaagcaa 
      tccagctca tggcgataa aatgtttca ccactttct 
      ctcaaagat tcagatgaa tcctagtca gattttgag 
      gtataatag ggacagcgc cccactgct cttgtaccc 
      tttacaagg gcttcaaac gacgtgcaa ttactgtaa 
      aggcgattt agttttcga atttcaagt tttgccaac 
      gattattcc aaagctgaa attggtggg cgatgccaa 
      acattgttc aagactgga gttcgggac tgttttagt 
      atttacatg tcgcaagag ttgctggtc aaccgatac 
      gaatacgag acatgagac tgtcattgt acaatcaga 
      agtttactg aatattatg caatatcgc ggcgaaaaa 
      attccacca ttgcttgcc actctttgt ttgttgtaa 
      tttcgtgtt tattacatc acaaagcga ttagggcgg 
      gtcatactt ttcttccaa aaatagaga atgtaacgt 
      aattattgt agtctcagg aattgtgca attcgcaag 
      ttttttgcc ttcataaaa atattacag ttggtgtta 
      cggtaattt tatgctctt taacgtagg cgctccgaa 
      actattttc tgagt
    • >TC030996 RC
      ccggggagg ctttttttt ttaattttt ttgatttcg 
      aaattattt caggtggtg ccgtaggcc ggctgcact 
      cgccctcgg ccaccgcgg aaatcgagg gcgacgaag 
      gcgaagttg tgaccatcg atagccccg ttatgcccc 
      aacaagaca tcgttgccc ttctgcagg aagccaccc 
      ctttaccgc agttgcacg gggaccccc gcaaatcct 
      ccctttttc aattcattt catcgttca tgggcgtgt 
      gggaggaaa ccgctggtg cgtccacca tcagcgata 
      gacacgtta aagaactgt tacaagaat atcaacaga 
      aaaacgaag acgatatga atttagtga gtcctgaca 
      ctggagggg cgtccgcac aaatcgccg ataacgtgt 
      acgaaaagt acgaaaaaa gcaatccag ctcatggcg 
      ataaaatgt ttcaccact ttctctcaa agattcaga 
      tgaatccta gtcagattt tgaggtata atagggaca 
      gcgccccac tgctcttgt accctttac aagggcttc 
      aaacgacgt gcaattact gtaaaggcg atttagttt 
      tcgaatttc aagttttgc caacgatta ttccaaagc 
      tgaaattgg tgggcgatg ccaaacatt gttcaagac 
      tggagttcg ggactgttt tagtattta catgtcgca 
      agagttgct ggtcaaccg atacgaata cgagacatg 
      agactgtca ttgtacaat cagaagttt actgaatat 
      tatgcaata tcgcggcga aaaaattcc accattgct 
      tgccactct ttgtttgtt gtaatttcg tgtttatta 
      catcacaaa gcgattagg gcgggtcat acttttctt 
      ccaaaaata gagaatgta acgtaatta ttgtagtct 
      caggaattg tgcaattcg caagttttt tgccttcat 
      aaaaatatt acagttggt gttacggta attttatgc 
      tctttaacg taggcgctc cgaaactat tttctgagt