General information | |||
TC030996
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10113006..10116223 | |
Sequence information | |||
Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC030996 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03035 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_09647 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 2
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC030996 RA
MAQNNIKVL LGFEDELIT EKYKNQVNF TTNKIGGKP DLPSNIKLE PPICPLCQL PRPLVVQVY APLESSPYH RTLYLFACI NPNCWNQSE SWICIRVQS QEKLIEHEE PSAAVTSKT SVTDWCADA DDWDDNNAN MNEENGNLI NNIERVSDE DDESCSFEE SVRTGLGNL TVDDRNANN GAFGKCLF
- >TC030996 RB
MAQNNIKVL LGFEDELIT EKYKNQVNF TTNKIGGKP DLPSNIKLE PPICPLCQL PRPLVVQVY APLESSPYH RTLYLFACI NPNCWNQSE SWICIRVQS QEKLIEHEE PSAAVTSKT SVTDWCADA DDWDDNNAN MNEENGNLI NNIERVSDE DDESCSFEE SVRTGLGNL TVDDRNANN GAFGGAVGR LHSPSATAE IEGDEGEVV TIDSPVMPQ QDIVALLQE ATPLPQLHG DPRKSSLFQ FISSFMGVW EETAGASTI SDRHVKELL QEYQQKNED DMNLVSPDT GGASAQIAD NVYEKYEKS NPAHGDKMF HHFLSKIQM NPSQILRYN RDSAPLLLY PLQGLQTTC NYCKGDLVF EFQVLPTII PKLKLVGDA KHCSRLEFG TVLVFTCRK SCWSTDTNT RHETVIVQS EVY
- >TC030996 RC
MPQQDIVAL LQEATPLPQ LHGDPRKSS LFQFISSFM GVWEETAGA STISDRHVK ELLQEYQQK NEDDMNLVS PDTGGASAQ IADNVYEKY EKSNPAHGD KMFHHFLSK IQMNPSQIL RYNRDSAPL LLYPLQGLQ TTCNYCKGD LVFEFQVLP TIIPKLKLV GDAKHCSRL EFGTVLVFT CRKSCWSTD TNTRHETVI VQSEVY
- >TC030996 RA
atggcccaa aataatatt aaagtactg ctcggtttt gaagacgaa ctcattact gagaaatat aaaaaccaa gttaacttc accaccaat aaaattgga ggaaaacct gatttacca agcaatatc aagcttgag ccgccaatc tgtcccctg tgccaacta ccaaggcct ttggtagtc caggtctat gcacctctg gagtcctcc ccataccac cgaacttta tacctattc gcttgcata aatccaaat tgttggaat caaagcgaa agctggatt tgtattcgg gtccagtcg caggaaaaa ctaattgaa catgaagaa ccatcagct gctgtaact tccaaaact agtgtaact gactggtgt gcagatgct gatgattgg gatgataac aacgccaac atgaacgaa gaaaacggc aatttaata aataacatt gaacgagtt tcggacgaa gatgacgaa agttgcagt tttgaagag tcagtgagg acgggactt gggaattta acagtggac gatagaaat gcaaataat ggagcgttt ggtaagtgt ttattttga
- >TC030996 RB
atggcccaa aataatatt aaagtactg ctcggtttt gaagacgaa ctcattact gagaaatat aaaaaccaa gttaacttc accaccaat aaaattgga ggaaaacct gatttacca agcaatatc aagcttgag ccgccaatc tgtcccctg tgccaacta ccaaggcct ttggtagtc caggtctat gcacctctg gagtcctcc ccataccac cgaacttta tacctattc gcttgcata aatccaaat tgttggaat caaagcgaa agctggatt tgtattcgg gtccagtcg caggaaaaa ctaattgaa catgaagaa ccatcagct gctgtaact tccaaaact agtgtaact gactggtgt gcagatgct gatgattgg gatgataac aacgccaac atgaacgaa gaaaacggc aatttaata aataacatt gaacgagtt tcggacgaa gatgacgaa agttgcagt tttgaagag tcagtgagg acgggactt gggaattta acagtggac gatagaaat gcaaataat ggagcgttt ggtggtgcc gtaggccgg ctgcactcg ccctcggcc accgcggaa atcgagggc gacgaaggc gaagttgtg accatcgat agccccgtt atgccccaa caagacatc gttgccctt ctgcaggaa gccacccct ttaccgcag ttgcacggg gacccccgc aaatcctcc ctttttcaa ttcatttca tcgttcatg ggcgtgtgg gaggaaacc gctggtgcg tccaccatc agcgataga cacgttaaa gaactgtta caagaatat caacagaaa aacgaagac gatatgaat ttagtgagt cctgacact ggaggggcg tccgcacaa atcgccgat aacgtgtac gaaaagtac gaaaaaagc aatccagct catggcgat aaaatgttt caccacttt ctctcaaag attcagatg aatcctagt cagattttg aggtataat agggacagc gccccactg ctcttgtac cctttacaa gggcttcaa acgacgtgc aattactgt aaaggcgat ttagttttc gaatttcaa gttttgcca acgattatt ccaaagctg aaattggtg ggcgatgcc aaacattgt tcaagactg gagttcggg actgtttta gtatttaca tgtcgcaag agttgctgg tcaaccgat acgaatacg agacatgag actgtcatt gtacaatca gaagtttac tga
- >TC030996 RC
atgccccaa caagacatc gttgccctt ctgcaggaa gccacccct ttaccgcag ttgcacggg gacccccgc aaatcctcc ctttttcaa ttcatttca tcgttcatg ggcgtgtgg gaggaaacc gctggtgcg tccaccatc agcgataga cacgttaaa gaactgtta caagaatat caacagaaa aacgaagac gatatgaat ttagtgagt cctgacact ggaggggcg tccgcacaa atcgccgat aacgtgtac gaaaagtac gaaaaaagc aatccagct catggcgat aaaatgttt caccacttt ctctcaaag attcagatg aatcctagt cagattttg aggtataat agggacagc gccccactg ctcttgtac cctttacaa gggcttcaa acgacgtgc aattactgt aaaggcgat ttagttttc gaatttcaa gttttgcca acgattatt ccaaagctg aaattggtg ggcgatgcc aaacattgt tcaagactg gagttcggg actgtttta gtatttaca tgtcgcaag agttgctgg tcaaccgat acgaatacg agacatgag actgtcatt gtacaatca gaagtttac tga
- >TC030996 RA
tacgagtag tgaataagt tttgttgaa taaatttat cagctgatg ctgaaacat ggcccaaaa taatattaa agtactgct cggttttga agacgaact cattactga gaaatataa aaaccaagt taacttcac caccaataa aattggagg aaaacctga tttaccaag caatatcaa gcttgagcc gccaatctg tcccctgtg ccaactacc aaggccttt ggtagtcca ggtctatgc acctctgga gtcctcccc ataccaccg aactttata cctattcgc ttgcataaa tccaaattg ttggaatca aagcgaaag ctggatttg tattcgggt ccagtcgca ggaaaaact aattgaaca tgaagaacc atcagctgc tgtaacttc caaaactag tgtaactga ctggtgtgc agatgctga tgattggga tgataacaa cgccaacat gaacgaaga aaacggcaa tttaataaa taacattga acgagtttc ggacgaaga tgacgaaag ttgcagttt tgaagagtc agtgaggac gggacttgg gaatttaac agtggacga tagaaatgc aaataatgg agcgtttgg taagtgttt attttgatg caacggtat atacaataa ataaaatta catcacaaa t
- >TC030996 RB
tacgagtag tgaataagt tttgttgaa taaatttat cagctgatg ctgaaacat ggcccaaaa taatattaa agtactgct cggttttga agacgaact cattactga gaaatataa aaaccaagt taacttcac caccaataa aattggagg aaaacctga tttaccaag caatatcaa gcttgagcc gccaatctg tcccctgtg ccaactacc aaggccttt ggtagtcca ggtctatgc acctctgga gtcctcccc ataccaccg aactttata cctattcgc ttgcataaa tccaaattg ttggaatca aagcgaaag ctggatttg tattcgggt ccagtcgca ggaaaaact aattgaaca tgaagaacc atcagctgc tgtaacttc caaaactag tgtaactga ctggtgtgc agatgctga tgattggga tgataacaa cgccaacat gaacgaaga aaacggcaa tttaataaa taacattga acgagtttc ggacgaaga tgacgaaag ttgcagttt tgaagagtc agtgaggac gggacttgg gaatttaac agtggacga tagaaatgc aaataatgg agcgtttgg tggtgccgt aggccggct gcactcgcc ctcggccac cgcggaaat cgagggcga cgaaggcga agttgtgac catcgatag ccccgttat gccccaaca agacatcgt tgcccttct gcaggaagc caccccttt accgcagtt gcacgggga cccccgcaa atcctccct ttttcaatt catttcatc gttcatggg cgtgtggga ggaaaccgc tggtgcgtc caccatcag cgatagaca cgttaaaga actgttaca agaatatca acagaaaaa cgaagacga tatgaattt agtgagtcc tgacactgg aggggcgtc cgcacaaat cgccgataa cgtgtacga aaagtacga aaaaagcaa tccagctca tggcgataa aatgtttca ccactttct ctcaaagat tcagatgaa tcctagtca gattttgag gtataatag ggacagcgc cccactgct cttgtaccc tttacaagg gcttcaaac gacgtgcaa ttactgtaa aggcgattt agttttcga atttcaagt tttgccaac gattattcc aaagctgaa attggtggg cgatgccaa acattgttc aagactgga gttcgggac tgttttagt atttacatg tcgcaagag ttgctggtc aaccgatac gaatacgag acatgagac tgtcattgt acaatcaga agtttactg aatattatg caatatcgc ggcgaaaaa attccacca ttgcttgcc actctttgt ttgttgtaa tttcgtgtt tattacatc acaaagcga ttagggcgg gtcatactt ttcttccaa aaatagaga atgtaacgt aattattgt agtctcagg aattgtgca attcgcaag ttttttgcc ttcataaaa atattacag ttggtgtta cggtaattt tatgctctt taacgtagg cgctccgaa actattttc tgagt
- >TC030996 RC
ccggggagg ctttttttt ttaattttt ttgatttcg aaattattt caggtggtg ccgtaggcc ggctgcact cgccctcgg ccaccgcgg aaatcgagg gcgacgaag gcgaagttg tgaccatcg atagccccg ttatgcccc aacaagaca tcgttgccc ttctgcagg aagccaccc ctttaccgc agttgcacg gggaccccc gcaaatcct ccctttttc aattcattt catcgttca tgggcgtgt gggaggaaa ccgctggtg cgtccacca tcagcgata gacacgtta aagaactgt tacaagaat atcaacaga aaaacgaag acgatatga atttagtga gtcctgaca ctggagggg cgtccgcac aaatcgccg ataacgtgt acgaaaagt acgaaaaaa gcaatccag ctcatggcg ataaaatgt ttcaccact ttctctcaa agattcaga tgaatccta gtcagattt tgaggtata atagggaca gcgccccac tgctcttgt accctttac aagggcttc aaacgacgt gcaattact gtaaaggcg atttagttt tcgaatttc aagttttgc caacgatta ttccaaagc tgaaattgg tgggcgatg ccaaacatt gttcaagac tggagttcg ggactgttt tagtattta catgtcgca agagttgct ggtcaaccg atacgaata cgagacatg agactgtca ttgtacaat cagaagttt actgaatat tatgcaata tcgcggcga aaaaattcc accattgct tgccactct ttgtttgtt gtaatttcg tgtttatta catcacaaa gcgattagg gcgggtcat acttttctt ccaaaaata gagaatgta acgtaatta ttgtagtct caggaattg tgcaattcg caagttttt tgccttcat aaaaatatt acagttggt gttacggta attttatgc tctttaacg taggcgctc cgaaactat tttctgagt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
