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Gene information
General information
TC000508 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10480776..10482848
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000508
    iBeetle screen iB_03034

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 25.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000508 RA
      MSKVILGGG LGGLSAAYY LKKFSPNQA VTLIESSSR 
      TGGWIKSSV QENGAIFEQ GPRTIRPRG DAGANTLQL 
      IEELNLSDQ IVPITSGHP AAKNRLIYA NGSLHLLPS 
      SLTGLFRKQ EPFSKPLIL HLLNDLKAR RKEVKDESI 
      YDFIGRRFG SEVADYLIS PLICGICAG NAKEISVKF 
      LMKQLFEYE QKHGSVSKG LLTSLFAKS KRPTVLCET 
      ALRARSEKW NIYSFKNGC ETLPIALRN NIVSNGVNI 
      KFNTECTEI ITDKGQVIL NLFNNETIK CDHLVGAIP 
      ANVLGRLLQ KQHPELAQC LKKLTKNVS VAVVNLLFK 
      ENFIKNPAF GLLVVPKEQ LPVLGVIYD SCCFPVENC 
      GTVLTVMMG GYWFEEHFG TNPQENVLL DVAVKHVKR 
      ILNCDHDPF NYKVNILKQ CLPQYVVGH DENLDLING 
      YIRTHKLPI SLCGASYYG VGINDVILS AKNAVKDL
    CDS sequence
    • >TC000508 RA
      atgagcaaa gtgatcctc ggcgggggc ttgggtggc 
      ctgtctgct gcctactac ttgaagaag ttttcgcct 
      aaccaagct gttacttta attgagtct tcgtcacgt 
      accgggggc tggatcaag tcaagtgtg caagaaaat 
      ggggctatt tttgaacaa gggcctcgt accatacgt 
      ccccggggg gatgcaggc gctaatact ttacaactg 
      attgaagaa ctaaattta agcgatcaa atcgtccct 
      ataacttcg ggacaccct gctgctaaa aatcggctc 
      atttacgcc aacggttct ttgcatttg ttgccgtcg 
      tctttgact ggtttgttt aggaaacaa gagccgttt 
      tcaaagcct ttgattctt cacttgttg aatgatttg 
      aaagcgagg aggaaggaa gtgaaggat gagtcaatt 
      tatgatttt atcggtcgc agatttggt agtgaagtg 
      gctgattat ttgatcagt ccgttgatt tgtggcatt 
      tgtgccgga aacgccaag gaaattagt gtgaagttt 
      ttaatgaaa cagttgttt gaatacgaa caaaaacat 
      ggaagtgta tcaaagggg ctccttaca agtttgttt 
      gcaaagtca aaacgacca actgttctg tgtgaaact 
      gccctcaga gctagaagt gagaaatgg aatatttat 
      tcctttaaa aatgggtgt gaaacgtta cccatagcc 
      ttaagaaat aatattgtt tcaaatggg gttaatatt 
      aaatttaat accgaatgt acggaaatt ataacggac 
      aagggccaa gtaatacta aatttattt aataacgaa 
      acaattaag tgtgatcac ttagtaggt gcaatacct 
      gcaaacgtt ttaggacgt cttttacaa aaacagcac 
      ccggaactt gcccagtgt ttgaaaaaa ctgacaaaa 
      aacgtgtca gtggctgtt gttaattta ctttttaag 
      gagaatttt attaaaaat ccagccttt ggtttattg 
      gttgttcct aaagaacaa cttcctgtg ctaggtgtt 
      atttacgat agttgttgt ttccctgtc gaaaactgt 
      ggaacggtt ttaaccgta atgatgggt ggttactgg 
      tttgaggaa cattttggc acaaatccg caagaaaat 
      gtgttgctg gatgttgcc gtaaaacac gtgaaacgt 
      atcttaaat tgtgatcac gatccgttt aattataaa 
      gtgaatatt ttaaagcag tgtttgcct caatatgtt 
      gtgggtcat gacgaaaat ttggacctg attaatggt 
      tacataagg acgcataaa ttaccaatt tctttgtgc 
      ggtgcctct tattacggt gtggggatt aatgatgta 
      attttgtcg gcgaaaaat gccgtaaag gatttgtaa
    mRNA sequence
    • >TC000508 RA
      ctatttggg tacagctgc cggttcaca tgatctcaa 
      catgacaag aagggaaga cgtcattta tcatgagca 
      aagtgatcc tcggcgggg gcttgggtg gcctgtctg 
      ctgcctact acttgaaga agttttcgc ctaaccaag 
      ctgttactt taattgagt cttcgtcac gtaccgggg 
      gctggatca agtcaagtg tgcaagaaa atggggcta 
      tttttgaac aagggcctc gtaccatac gtccccggg 
      gggatgcag gcgctaata ctttacaac tgattgaag 
      aactaaatt taagcgatc aaatcgtcc ctataactt 
      cgggacacc ctgctgcta aaaatcggc tcatttacg 
      ccaacggtt ctttgcatt tgttgccgt cgtctttga 
      ctggtttgt ttaggaaac aagagccgt tttcaaagc 
      ctttgattc ttcacttgt tgaatgatt tgaaagcga 
      ggaggaagg aagtgaagg atgagtcaa tttatgatt 
      ttatcggtc gcagatttg gtagtgaag tggctgatt 
      atttgatca gtccgttga tttgtggca tttgtgccg 
      gaaacgcca aggaaatta gtgtgaagt ttttaatga 
      aacagttgt ttgaatacg aacaaaaac atggaagtg 
      tatcaaagg ggctcctta caagtttgt ttgcaaagt 
      caaaacgac caactgttc tgtgtgaaa ctgccctca 
      gagctagaa gtgagaaat ggaatattt attccttta 
      aaaatgggt gtgaaacgt tacccatag ccttaagaa 
      ataatattg tttcaaatg gggttaata ttaaattta 
      ataccgaat gtacggaaa ttataacgg acaagggcc 
      aagtaatac taaatttat ttaataacg aaacaatta 
      agtgtgatc acttagtag gtgcaatac ctgcaaacg 
      ttttaggac gtcttttac aaaaacagc acccggaac 
      ttgcccagt gtttgaaaa aactgacaa aaaacgtgt 
      cagtggctg ttgttaatt tacttttta aggagaatt 
      ttattaaaa atccagcct ttggtttat tggttgttc 
      ctaaagaac aacttcctg tgctaggtg ttatttacg 
      atagttgtt gtttccctg tcgaaaact gtggaacgg 
      ttttaaccg taatgatgg gtggttact ggtttgagg 
      aacattttg gcacaaatc cgcaagaaa atgtgttgc 
      tggatgttg ccgtaaaac acgtgaaac gtatcttaa 
      attgtgatc acgatccgt ttaattata aagtgaata 
      ttttaaagc agtgtttgc ctcaatatg ttgtgggtc 
      atgacgaaa atttggacc tgattaatg gttacataa 
      ggacgcata aattaccaa tttctttgt gcggtgcct 
      cttattacg gtgtgggga ttaatgatg taattttgt 
      cggcgaaaa atgccgtaa aggatttgt aacagaaac 
      gcgaatgtt tttataaat gatttattg ttgtcttgt 
      acaaaagaa ataaattgt tacaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY