Status
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Gene information
General information
TC000502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10543910..10546308
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000502
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03032

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000502 RA
      MLIPQEALN SVKCNLCDK FLSYFPIYT CEKNLPICG 
      RCSAILNDT NFRRATLFE QVAQYLKFP CIYHTAGCV 
      ENLFPDEVP NHEENCPYK IIACSQECM WQGSVNELL 
      EHFEDTHPN AILRNSEFE ISFLNSYDT YSLVVYEDE 
      LFSFKRKFD ITENVLKCS VFCYKMLDP VTKYNYKIV 
      VQNGTGTES VEFAPKSAD TFLEENVTE ISVENIRDR 
      LKDPSVIIG TIQIFDQKE VSSLKKKLN PDINFEMLA 
      ELECPVCLH YIIPPIFQC VTGHSICGT CKEQITQCP 
      LCQQDIKNT QNFTLEKMA FLLTYPCMN SENGCDFAD 
      KPGKLKQHQ KYCLYGTHH CPLKDYESC KWKGSAKNI 
      YRHIQDVHH DNMLEVDTV RLFLDGAYF QQEENTCYI 
      MKYAEAIFK LHYRYYREC FYWAMQLIG PPEEAKNYK 
      FEIDICDNN NNSRRLFLR NFCSSLKEK DDSFTDPDQ 
      YVFLTLNQI RSFLSEMLT FTVRIVK
    CDS sequence
    • >TC000502 RA
      atgttgata ccgcaggaa gcccttaat tcggtaaag 
      tgcaatctc tgtgacaaa ttcctctcg tacttccca 
      atttacaca tgtgagaaa aatctaccg atttgtggg 
      cgttgctct gcgattttg aacgatact aatttccga 
      agagctaca ctctttgaa caagtcgcc cagtattta 
      aaatttcca tgtatttat catacggca ggctgtgtc 
      gaaaaccta ttcccagac gaagtccca aatcacgaa 
      gagaactgc ccttacaaa ataatcgcg tgctcgcaa 
      gaatgcatg tggcaaggg agcgtcaac gaactcttg 
      gaacatttt gaagacact catccaaat gccattctg 
      agaaatagc gagtttgaa ataagtttt cttaattcc 
      tacgacacg tacagtctt gtggtgtac gaagacgag 
      cttttcagt ttcaaacga aagtttgat atcacggaa 
      aatgtgctg aaatgctcc gtcttctgc tacaaaatg 
      ctggatcct gtgaccaaa tacaactat aaaattgtc 
      gtccagaac gggaccggg accgaatcg gtggaattt 
      gccccaaag tccgctgat acgttctta gaggagaat 
      gtcacggaa attagcgtt gaaaacatt agggatcgc 
      ctaaaggac ccatcagtg atcatcggc actattcaa 
      atatttgat cagaaggaa gtttccagt ttaaagaaa 
      aaactaaac ccggatata aattttgaa atgttagcc 
      gaactggag tgcccagtg tgcctccac tacatcatc 
      cctccgatt ttccagtgt gttaccggc catagtatc 
      tgcggtact tgcaaggaa caaatcaca caatgtcct 
      ctttgccag caggacatc aaaaacacc caaaatttc 
      acgctcgaa aaaatggcc ttccttctc acctaccct 
      tgcatgaac tccgaaaac ggctgtgac tttgccgac 
      aagcctggc aaactcaaa cagcaccaa aagtactgt 
      ctttacggg acgcaccac tgcccgctt aaagactat 
      gaaagctgc aaatggaaa ggctctgcc aaaaatata 
      taccgccac attcaggac gtccatcat gacaacatg 
      ctggaagtt gacacagtg aggctgttc ctcgacggt 
      gcctacttc cagcaagag gaaaacact tgctacata 
      atgaaatat gcggaagct atattcaaa ttgcactac 
      cggtactac agggagtgc ttctattgg gccatgcag 
      ctgatcggg ccccccgaa gaagccaag aactataag 
      tttgaaatc gacatttgt gataacaac aacaacagc 
      cgcaggcta tttttgagg aatttttgc tcgagtttg 
      aaggagaag gacgacagt tttactgat cccgaccag 
      tacgtgttt cttacgctc aaccaaatt aggtccttt 
      ctgtccgag atgttgact ttcacagtg cgaattgtc 
      aagtaa
    mRNA sequence
    • >TC000502 RA
      cgaaaagca agcaacaca caacacgta caatttatc 
      attttctgt gtttcacac agctgataa gtgattctg 
      attacagat tacagttgc attttaata taacagtag 
      taacacagt tccaccttt tttgtgtaa aagtcggaa 
      gtgtccttt gtcctgata gattcgtgt gtcaccatg 
      ttgataccg caggaagcc cttaattcg gtaaagtgc 
      aatctctgt gacaaattc ctctcgtac ttcccaatt 
      tacacatgt gagaaaaat ctaccgatt tgtgggcgt 
      tgctctgcg attttgaac gatactaat ttccgaaga 
      gctacactc tttgaacaa gtcgcccag tatttaaaa 
      tttccatgt atttatcat acggcaggc tgtgtcgaa 
      aacctattc ccagacgaa gtcccaaat cacgaagag 
      aactgccct tacaaaata atcgcgtgc tcgcaagaa 
      tgcatgtgg caagggagc gtcaacgaa ctcttggaa 
      cattttgaa gacactcat ccaaatgcc attctgaga 
      aatagcgag tttgaaata agttttctt aattcctac 
      gacacgtac agtcttgtg gtgtacgaa gacgagctt 
      ttcagtttc aaacgaaag tttgatatc acggaaaat 
      gtgctgaaa tgctccgtc ttctgctac aaaatgctg 
      gatcctgtg accaaatac aactataaa attgtcgtc 
      cagaacggg accgggacc gaatcggtg gaatttgcc 
      ccaaagtcc gctgatacg ttcttagag gagaatgtc 
      acggaaatt agcgttgaa aacattagg gatcgccta 
      aaggaccca tcagtgatc atcggcact attcaaata 
      tttgatcag aaggaagtt tccagttta aagaaaaaa 
      ctaaacccg gatataaat tttgaaatg ttagccgaa 
      ctggagtgc ccagtgtgc ctccactac atcatccct 
      ccgattttc cagtgtgtt accggccat agtatctgc 
      ggtacttgc aaggaacaa atcacacaa tgtcctctt 
      tgccagcag gacatcaaa aacacccaa aatttcacg 
      ctcgaaaaa atggccttc cttctcacc tacccttgc 
      atgaactcc gaaaacggc tgtgacttt gccgacaag 
      cctggcaaa ctcaaacag caccaaaag tactgtctt 
      tacgggacg caccactgc ccgcttaaa gactatgaa 
      agctgcaaa tggaaaggc tctgccaaa aatatatac 
      cgccacatt caggacgtc catcatgac aacatgctg 
      gaagttgac acagtgagg ctgttcctc gacggtgcc 
      tacttccag caagaggaa aacacttgc tacataatg 
      aaatatgcg gaagctata ttcaaattg cactaccgg 
      tactacagg gagtgcttc tattgggcc atgcagctg 
      atcgggccc cccgaagaa gccaagaac tataagttt 
      gaaatcgac atttgtgat aacaacaac aacagccgc 
      aggctattt ttgaggaat ttttgctcg agtttgaag 
      gagaaggac gacagtttt actgatccc gaccagtac 
      gtgtttctt acgctcaac caaattagg tcctttctg 
      tccgagatg ttgactttc acagtgcga attgtcaag 
      taattggta tttttttat ttagagata agttacgtt 
      cgttcattt tgttacttt attatcgat acatgtatt 
      ttaatttcc gctaaacca ataaagttg tgattatta 
      a
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