Status
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Gene information
General information
TC030999
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000485,TC000484
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10706079..10711091
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030999
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030999 RA
      MNEAAALPH GPPMNILPP TPAGLESLY TLCRKIYPD 
      QANPLQVTA LIKYWLGGP DPLDYISMY ANPGIPQEN 
      IPPHWHYIS FGLSDLHGD GRVHDVSGA NGVSGFGFE 
      LTFRLKREP EETAPPTWP ATLMQSLAK YVFQSGNTL 
      CAGDHVSWH CALDSSESR IQHMLMTTD VHLKSIQTP 
      FGSVDFVQI VGICAEELK AAQQWNGAG VLDMITRIP 
      SAGGPWFIT DMRRGESIF ELDLNAHEE VERGFELEG 
      SNLSGVSAR CSWVEQDPA RIDIMHLKE KSDCDNFQA 
      QEDVTLLNS PQKPLQSGN FEQNRSSST NFKMDESTE 
      LLHIKKLEG VHLVFNLEA GSLLPLAIR GRVRHGRHF 
      TFKSVLGET AITLVAGTV TGTLVNSEH PYVAQGSWL 
      QILITDDLA QDMAVTFQV LATPEALSL PKTFSWPDK 
      NAGGPWFIT DMRRGESIF ELDLNAHEE VERGFELEG 
      SNLSGVSAR CSWVEQDPA RIDIMHLKE KSDCDNFQA 
      PILKLYQMD SVQEVVVPT FVNAHLFWI IDKKDLLLR 
      AAMEKVMRS NRCNKIKGL GEVSNDQIV AVNVEGVWT 
      RAIVKDVEK DFGSDFKYK CWLPDYGTM RVVNNLFHL 
      PEGLEECPH LARQASLEN VVNLMTDLV LGDSGYEFQ 
      FKTTTDDIE GVQRVALDC LEAAEKLFF EAKRQDEFN 
      SYGDITCVY QENAKESLT EYLIDKKLI RIDEALFKT 
      LEPSRTTFK ENYFQHIVN VQQPLVKTE RNFNRIIFN 
      QNEGVSMNF FKRNFAPDE DSSDW
    CDS sequence
    • >TC030999 RA
      atgaatgaa gctgctgct ctgccccat ggaccccca 
      atgaacatt ctgccaccg acaccagca ggtttggag 
      tctttgtac acgctatgt aggaaaatt taccccgat 
      caagccaac ccccttcaa gtcacggcc ctcatcaag 
      tactggctg ggagggccc gatccttta gattacata 
      tcaatgtat gcaaatcca ggcattcca caagaaaac 
      attcctcca cattggcac tacataagt tttggtctc 
      tcagacctt cacggtgac gggcgagtg cacgacgtg 
      tcaggagct aatggtgtt tcaggtttt ggttttgag 
      ctaacattt aggctaaaa cgggagcct gaagagacg 
      gcaccgccc acctggccc gcaacttta atgcaatct 
      ttagcgaaa tacgttttt cagtcaggc aacactctt 
      tgtgctggt gaccacgtt tcctggcac tgcgcgtta 
      gatagcagc gagtcacga atacagcac atgttgatg 
      accacagac gtgcatttg aagtcaata cagacgcct 
      tttggttct gtcgatttc gtccaaatt gttggtatt 
      tgcgccgag gagctgaaa gcagcccag cagtggaac 
      ggggccggg gtgcttgat atgataact agaataccg 
      tctgctggg gggccttgg tttatcacg gatatgagg 
      cgaggggag agcattttt gagcttgat ttaaacgcg 
      cacgaggaa gtcgagcga gggtttgag ttagagggc 
      tccaatttg agcggagtc agcgctagg tgttcatgg 
      gttgagcaa gaccctgct cgtattgac ataatgcat 
      ttgaaagaa aagtccgat tgtgacaat ttccaagct 
      caagaggac gtaactctg ttaaattca cctcaaaaa 
      ccgctacag agtggcaac tttgaacaa aaccgctcc 
      agtagcacc aatttcaaa atggacgag tcaactgag 
      ttgttgcac ataaagaaa ttagagggc gtccatttg 
      gtttttaat ttggaggct gggagttta ctccctttg 
      gccatcagg gggcgcgta cgacatggt agacatttc 
      acatttaag tcagttttg ggtgaaact gccataaca 
      ctagtggca ggtacggtt acagggacg ttggtgaac 
      tcagaacac ccttatgta gctcagggt tcatggcta 
      caaatactt attaccgac gatttagct caggatatg 
      gcggttact tttcaagtt ctggccact ccagaggcg 
      ctttcttta ccaaaaaca ttctcatgg cccgacaaa 
      aatgctggg gggccttgg tttatcacg gatatgagg 
      cgaggggag agcattttt gagcttgat ttaaacgcg 
      cacgaggaa gtcgagcga gggtttgag ttagagggc 
      tccaatttg agcggagtc agcgctagg tgttcatgg 
      gttgagcaa gaccctgct cgtattgac ataatgcat 
      ttgaaagaa aagtccgat tgtgacaat ttccaagct 
      ccaattttg aaattgtac caaatggat tcagtccag 
      gaagtggtt gtcccaaca ttcgttaat gcgcactta 
      ttttggata atagacaag aaagacttg cttttacgc 
      gcagcgatg gaaaaagta atgagaagt aatagatgt 
      aataaaatt aagggtctt ggcgaagtt tctaatgat 
      cagattgtg gcagttaat gttgagggt gtgtggaca 
      agagcgata gtcaaagac gttgaaaaa gattttgga 
      agtgatttt aaatacaaa tgttggctt cctgattat 
      ggcactatg agagtagtg aataattta tttcattta 
      cctgaaggg ttagaagag tgtccacat cttgccaga 
      caggccagt ttggaaaac gttgttaat ttaatgacg 
      gatttagtt cttggtgat agtggatac gaattccaa 
      ttcaaaaca acaactgat gatattgaa ggtgttcaa 
      agagtcgct cttgactgt cttgaggca gcggaaaag 
      ttattcttt gaagcaaaa agacaagac gagtttaac 
      tcttatggg gatatcacc tgtgtttat caagagaat 
      gccaaagaa tcacttaca gaatattta attgataag 
      aagcttata agaattgac gaagcctta ttcaaaaca 
      ttggaaccc agtagaact acctttaag gaaaactac 
      tttcaacat atagtaaat gtgcaacaa ccgctagtt 
      aagacggaa agaaacttt aacagaatt attttcaac 
      caaaatgag ggtgtttca atgaacttc ttcaaacga 
      aactttgcg ccggatgaa gactccagt gattggtaa
    mRNA sequence
    • >TC030999 RA
      aaagttgtt cctgcattc ttgaaatat tttctttta 
      ttggtctgg acggaagtg taaaatgtt ctaaagtgg 
      taacaaacg gctaaatat aatactttc tgccgattt 
      ctaaacaat gaatgaagc tgctgctct gccccatgg 
      acccccaat gaacattct gccaccgac accagcagg 
      tttggagtc tttgtacac gctatgtag gaaaattta 
      ccccgatca agccaaccc ccttcaagt cacggccct 
      catcaagta ctggctggg agggcccga tcctttaga 
      ttacatatc aatgtatgc aaatccagg cattccaca 
      agaaaacat tcctccaca ttggcacta cataagttt 
      tggtctctc agaccttca cggtgacgg gcgagtgca 
      cgacgtgtc aggagctaa tggtgtttc aggttttgg 
      ttttgagct aacatttag gctaaaacg ggagcctga 
      agagacggc accgcccac ctggcccgc aactttaat 
      gcaatcttt agcgaaata cgtttttca gtcaggcaa 
      cactctttg tgctggtga ccacgtttc ctggcactg 
      cgcgttaga tagcagcga gtcacgaat acagcacat 
      gttgatgac cacagacgt gcatttgaa gtcaataca 
      gacgccttt tggttctgt cgatttcgt ccaaattgt 
      tggtatttg cgccgagga gctgaaagc agcccagca 
      gtggaacgg ggccggggt gcttgatat gataactag 
      aataccgtc tgctggggg gccttggtt tatcacgga 
      tatgaggcg aggggagag catttttga gcttgattt 
      aaacgcgca cgaggaagt cgagcgagg gtttgagtt 
      agagggctc caatttgag cggagtcag cgctaggtg 
      ttcatgggt tgagcaaga ccctgctcg tattgacat 
      aatgcattt gaaagaaaa gtccgattg tgacaattt 
      ccaagctca agaggacgt aactctgtt aaattcacc 
      tcaaaaacc gctacagag tggcaactt tgaacaaaa 
      ccgctccag tagcaccaa tttcaaaat ggacgagtc 
      aactgagtt gttgcacat aaagaaatt agagggcgt 
      ccatttggt ttttaattt ggaggctgg gagtttact 
      ccctttggc catcagggg gcgcgtacg acatggtag 
      acatttcac atttaagtc agttttggg tgaaactgc 
      cataacact agtggcagg tacggttac agggacgtt 
      ggtgaactc agaacaccc ttatgtagc tcagggttc 
      atggctaca aatacttat taccgacga tttagctca 
      ggatatggc ggttacttt tcaagttct ggccactcc 
      agaggcgct ttctttacc aaaaacatt ctcatggcc 
      cgacaaaaa tgctggggg gccttggtt tatcacgga 
      tatgaggcg aggggagag catttttga gcttgattt 
      aaacgcgca cgaggaagt cgagcgagg gtttgagtt 
      agagggctc caatttgag cggagtcag cgctaggtg 
      ttcatgggt tgagcaaga ccctgctcg tattgacat 
      aatgcattt gaaagaaaa gtccgattg tgacaattt 
      ccaagctcc aattttgaa attgtacca aatggattc 
      agtccagga agtggttgt cccaacatt cgttaatgc 
      gcacttatt ttggataat agacaagaa agacttgct 
      tttacgcgc agcgatgga aaaagtaat gagaagtaa 
      tagatgtaa taaaattaa gggtcttgg cgaagtttc 
      taatgatca gattgtggc agttaatgt tgagggtgt 
      gtggacaag agcgatagt caaagacgt tgaaaaaga 
      ttttggaag tgattttaa atacaaatg ttggcttcc 
      tgattatgg cactatgag agtagtgaa taatttatt 
      tcatttacc tgaagggtt agaagagtg tccacatct 
      tgccagaca ggccagttt ggaaaacgt tgttaattt 
      aatgacgga tttagttct tggtgatag tggatacga 
      attccaatt caaaacaac aactgatga tattgaagg 
      tgttcaaag agtcgctct tgactgtct tgaggcagc 
      ggaaaagtt attctttga agcaaaaag acaagacga 
      gtttaactc ttatgggga tatcacctg tgtttatca 
      agagaatgc caaagaatc acttacaga atatttaat 
      tgataagaa gcttataag aattgacga agccttatt 
      caaaacatt ggaacccag tagaactac ctttaagga 
      aaactactt tcaacatat agtaaatgt gcaacaacc 
      gctagttaa gacggaaag aaactttaa cagaattat 
      tttcaacca aaatgaggg tgtttcaat gaacttctt 
      caaacgaaa ctttgcgcc ggatgaaga ctccagtga 
      ttggtaaat ttggtaagt aagaatttt ttcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY