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Gene information
General information
TC000483 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10732243..10733441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000483
    iBeetle screen iB_03029

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000483 RA
      MLHHYNYST SLSHFLSYH HLDEQSENE 
      HQSGALCEE QEDPRCRSN SSATNNKSF 
      TIAAILGLK NDSNQDDSA ADLSVVNLS 
      VHHPQGSNC NRLQLPVRH SSSAVSVTG 
      HYSVGHGCP QRQAVRGLK HLSSAVIQS 
      KKSCKSKRV RTIFTPEQL ERLEAEFER 
      QQYMVGPER LYLAHTLQL TEAQVKVWF 
      QNRRIKWRK HHLELTHQR LAVLKQQQL 
      LLDEAPDVQ DTNSSSTDT
    CDS sequence
    • >TC000483 RA
      atgctccac cattacaac tactcgacc tccctgtcc 
      catttcctg agctaccac cacctggac gagcagagc 
      gagaacgag caccagtcg ggcgccctc tgcgaggag 
      caggaggac ccccgctgc aggagcaac tcctccgcc 
      accaacaac aagtccttc accatcgcg gcgattttg 
      gggctgaag aacgactcg aatcaggac gattcggcg 
      gcagatttg agcgttgtt aatttgagc gtccaccac 
      ccgcagggg tcgaattgc aaccggttg cagcttccg 
      gtacgccac agttcttcg gcagtaagc gtgacgggg 
      cattactcg gtcgggcat gggtgcccc caaagacag 
      gcagtgcga ggattgaaa catctctcg tcggcggtg 
      atacagagt aaaaagagt tgtaaaagt aaacgagtt 
      cgaacgatt ttcaccccg gagcagctg gagcggctc 
      gaggcggag ttcgagaga cagcagtac atggtgggg 
      ccggaacgg ctctatttg gcccacacg ctgcagctg 
      actgaggcc caagtcaag gtttggttc cagaacagg 
      aggatcaag tggcggaag caccacttg gagctgacg 
      caccagcgg cttgcggtt ttgaagcaa cagcagctt 
      cttctcgac gaagcgccc gacgttcaa gacacgaac 
      agctcgagc accgacacg tga
    mRNA sequence
    • >TC000483 RA
      ccaggttga gggctgagg tcgggaaat cgcgtgggg 
      gtgtgtccg cacattaaa cccacccct gcggctgga 
      ggggttcaa tgatgagca ccggaacaa ctatacctt 
      gtgtcagtg agataacca acttgctct ctgccagct 
      ttatttcat taatgcact tattagcca tgtgtttcg 
      ccagtcttc agtcttaga ccggcttgt gtgcgtgtt 
      aagtgtttg acaaattcg aatttgcgg aatcatgtg 
      attattcct ccgaaaatg ctccaccat tacaactac 
      tcgacctcc ctgtcccat ttcctgagc taccaccac 
      ctggacgag cagagcgag aacgagcac cagtcgggc 
      gccctctgc gaggagcag gaggacccc cgctgcagg 
      agcaactcc tccgccacc aacaacaag tccttcacc 
      atcgcggcg attttgggg ctgaagaac gactcgaat 
      caggacgat tcggcggca gatttgagc gttgttaat 
      ttgagcgtc caccacccg caggggtcg aattgcaac 
      cggttgcag cttccggta cgccacagt tcttcggca 
      gtaagcgtg acggggcat tactcggtc gggcatggg 
      tgcccccaa agacaggca gtgcgagga ttgaaacat 
      ctctcgtcg gcggtgata cagagtaaa aagagttgt 
      aaaagtaaa cgagttcga acgattttc accccggag 
      cagctggag cggctcgag gcggagttc gagagacag 
      cagtacatg gtggggccg gaacggctc tatttggcc 
      cacacgctg cagctgact gaggcccaa gtcaaggtt 
      tggttccag aacaggagg atcaagtgg cggaagcac 
      cacttggag ctgacgcac cagcggctt gcggttttg 
      aagcaacag cagcttctt ctcgacgaa gcgcccgac 
      gttcaagac acgaacagc tcgagcacc gacacgtga 
      attgactta atcaagtgg aggctttga gcttgtaaa 
      tattgtgtg agaatgtag ctaattatt tcccaaacc 
      cgtggaatt agtttgtaa aatatgcgg cctgatttt 
      aaatacacc atttgattt taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY