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Gene information
General information
TC000482 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10738981..10740053
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000482
    iBeetle screen iB_03028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Comment: Females potentially too young.
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000482 RA
      MSFDDYKAV IDEGDTVIL YLTISQIYS IRAEAKTLS 
      KKGVEVENV FQTPYGALK CADLIGKPY GSKITLSKG 
      WGYVMQPTP ELWTQTLPH RTQIIYTPD ISMIILQLE 
      IAPGSIVVE SGTGSGSLS HALIRAVKP TGHLYTFDF 
      HKNRVELVR EEFAQHGLS DYVTVSQRD VCSSGFGDE 
      LENKADSVF LDLPHPWQA IPHTLNVIK ETGGRICSF 
      SPCIEQVQK SCEVLSKLG FQEIQTMEV LQTSYSVQT 
      RKLPVLDLD FLKSEKDGT ETKGKEREV VKCVTAVPP 
      ASMPGHTGY LTFASLPPV WARSLQISL NQAQEDNSP 
      DL
    CDS sequence
    • >TC000482 RA
      atgagtttc gacgattat aaagcagtg atagacgag 
      ggcgataca gtaattctt tatttgacg ataagccag 
      atctacagt attcgtgcc gaagctaaa actttaagt 
      aaaaaagga gtagaagta gagaatgtg ttccagacg 
      ccttatggg gctctcaag tgtgctgat ttgataggg 
      aagccctac ggttcaaag atcactctg agtaaaggt 
      tggggctac gtgatgcag cccaccccc gaattgtgg 
      acccaaacc ctgccccat cgaacccaa ataatctac 
      acgcctgat atcagcatg attatcctt cagctggaa 
      attgctcct ggtagtatc gttgtggag agtggtacc 
      gggagtggc tccctgtcc catgcccta atccgagct 
      gtgaaacca accggacac ttgtacact tttgatttc 
      cataaaaac agagtagaa ctagtccga gaagaattc 
      gcccagcat ggcttaagt gactatgtc accgtcagt 
      caacgggac gtttgctcc agtggattt ggggatgag 
      ttagagaat aaagcggat tcagtgttt ctagatttg 
      ccacatcct tggcaagca ataccacac actttgaac 
      gtaatcaaa gaaacaggg gggcgcatt tgctcattt 
      tcgccgtgt attgaacaa gtacaaaag agttgtgag 
      gttttgtca aaattaggg tttcaagaa atacagaca 
      atggaagtt ttgcaaact tcctactct gtgcagaca 
      aggaaattg ccggtgctt gatttggat ttccttaaa 
      agtgaaaag gatggaacg gagacaaag ggtaaagag 
      agggaagtt gtgaagtgt gttacggct gttccacct 
      gcaagtatg ccgggacat acgggctat ctaacgttc 
      gcctcgttg ccccccgtc tgggcaagg agtctccaa 
      atcagccta aatcaggcc caagaagac aattccccc 
      gatttataa
    mRNA sequence
    • >TC000482 RA
      attctgaaa taattcgcg atgagtttc gacgattat 
      aaagcagtg atagacgag ggcgataca gtaattctt 
      tatttgacg ataagccag atctacagt attcgtgcc 
      gaagctaaa actttaagt aaaaaagga gtagaagta 
      gagaatgtg ttccagacg ccttatggg gctctcaag 
      tgtgctgat ttgataggg aagccctac ggttcaaag 
      atcactctg agtaaaggt tggggctac gtgatgcag 
      cccaccccc gaattgtgg acccaaacc ctgccccat 
      cgaacccaa ataatctac acgcctgat atcagcatg 
      attatcctt cagctggaa attgctcct ggtagtatc 
      gttgtggag agtggtacc gggagtggc tccctgtcc 
      catgcccta atccgagct gtgaaacca accggacac 
      ttgtacact tttgatttc cataaaaac agagtagaa 
      ctagtccga gaagaattc gcccagcat ggcttaagt 
      gactatgtc accgtcagt caacgggac gtttgctcc 
      agtggattt ggggatgag ttagagaat aaagcggat 
      tcagtgttt ctagatttg ccacatcct tggcaagca 
      ataccacac actttgaac gtaatcaaa gaaacaggg 
      gggcgcatt tgctcattt tcgccgtgt attgaacaa 
      gtacaaaag agttgtgag gttttgtca aaattaggg 
      tttcaagaa atacagaca atggaagtt ttgcaaact 
      tcctactct gtgcagaca aggaaattg ccggtgctt 
      gatttggat ttccttaaa agtgaaaag gatggaacg 
      gagacaaag ggtaaagag agggaagtt gtgaagtgt 
      gttacggct gttccacct gcaagtatg ccgggacat 
      acgggctat ctaacgttc gcctcgttg ccccccgtc 
      tgggcaagg agtctccaa atcagccta aatcaggcc 
      caagaagac aattccccc gatttataa aacatacct 
      attttattt attttaagg acacgtatt tgtagtatt 
      ttaattttt aataaagtt tttttactg tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY