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Gene information
General information
TC000478 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10820855..10824197
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000478
    iBeetle screen iB_03027

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000478 RA
      MPKGSKIFL PCRSLGYVS NHIPLQVRY IKSRKENLI 
VTCVGKSFH TYGITHFGL LSVGGLHPE DITAMTADA 
FHVYTACNN EIYAWRRGT ELKHVYRGH KKPVHLMMP 
FGAHLISID EASSVKLWD IKDESVFLE LTFSNKTFH 
ITTICHPST YINKILLGS EQGEMQLWN VNNLKMIYS 
FKGWNSPIT CLEQAPAID VVAVGLACG KIKLHNLKF 
DETIMEFMQ DWGVVTSIS FRTDGSPIM ATGSVIGHI 
VFWDLEERR VASQLLSAH DGSVTGMIC LPSEPLILT 
SSPDNTLKL WIFDRSDGG ARLLRIREG HSAPPSHLR 
FHGANGHNI LSCAGDSTL RIFNTQTEQ FNKSLGKAS 
YNRKASKKR GRTAEDPLI MPPITQFTS EITREKEWD 
NIAAVHLGI PMVTTWSYD KVKMGELKL LPERFHKKK 
LVSSGFHDV AATCLCLTH CGNFVLVGY STGHIDRFN 
MQSGIWRDS YGAPKAHDA SVRGVTTDP LNQMTISGS 
SDCKIKFWK FKNKGANPL TVLTLDEPI SFFRSHSES 
SMLAVALED FDVYVVDID TRRIVRKFV GHTAQITDA 
TFSPDSRWL VTSSMDCSI RTWDIPSCH LVDEFSTEV 
ACVSLDISP TGEVLATAH VDYLGIFLW TNRTLYSKV 
SLKALTPSV QPPLVAFPE CLTEHHEEK SEVEESDDL 
EFISPEQIS NDLVTLSGF AASRWQNLL NLDVIKKRN 
KPKSPPKAP KAAPFFLPT VSSLTFQFD LSKPESESS 
KILTPSTLL NLTEFGKLL DKTRQDDDF SHVIEKLKS 
FGPSMIDFE IKSLAPEGG GSVEVMLQF LKSVEFMLK 
SNKDFELAE AYLGVFLKS HGTVVAAEE TLRNYLTNI 
QSCHNVVWN RLQDKLLYN ICVVQNLKM M
    CDS sequence
    • >TC000478 RA
      atgcctaaa ggcagcaaa atatttttg ccctgtcgg 
agcttaggg tacgtcagc aaccacatc ccgcttcag 
gtccgctac ataaaaagt cgcaaagaa aatctaata 
gtgacatgt gtggggaaa tcgtttcac acgtacgga 
ataacacac tttggcctg ttaagtgtc ggtggtttg 
caccccgaa gacataact gcgatgacc gctgatgcc 
tttcatgtg tacacagcg tgcaataat gagatttat 
gcctggaga agaggcact gagttgaaa catgtctat 
agaggacac aagaaaccg gttcatttg atgatgcct 
tttggggca catttgatt tcaattgat gaggccagt 
agtgttaaa ctgtgggat attaaggat gagagtgtt 
tttcttgaa ctcacgttt agtaacaag acattccat 
atcaccaca atctgccac cccagcact tatattaat 
aagattttg ttgggtagt gaacaggga gagatgcag 
ttatggaat gtcaataat ttgaaaatg atttattcc 
ttcaagggg tggaacagc cccattact tgcttggaa 
caagcgcct gcaattgat gtggttgct gttggtttg 
gcttgcggt aaaatcaaa ttacacaat ttaaaattc 
gacgagacc ataatggaa tttatgcaa gattggggc 
gtcgtcact tcgatatca tttaggacc gatggtagt 
cccataatg gcaacagga agtgtaata ggccatata 
gtattttgg gatttggaa gaaagacgc gtcgcgagc 
caactactt tctgcccac gacggcagc gttacagga 
atgatttgt ctcccgagt gaaccttta atcctaact 
tcgtctcca gacaatact ttaaaatta tggatcttt 
gacaggtca gacggtggt gccagactt ttaagaata 
agagaaggc cattcagca ccgccgtct caccttaga 
tttcacggt gctaacgga cacaacatt ttaagttgc 
gctggagat tcaacactg agaatattt aatacgcaa 
actgagcaa ttcaataaa agtctagga aaagcgtcg 
tacaacaga aaagcgtca aagaagcgc gggagaacc 
gccgaagac ccgctaatt atgccacca atcacacaa 
tttacttcc gaaattaca agggagaaa gaatgggat 
aatatcgca gcggtacat ttggggata cccatggtt 
accacatgg tcttacgat aaggtgaag atgggcgaa 
ttgaaattg cttcctgaa cggtttcac aagaaaaaa 
ctcgtttct agtgggttt cacgacgtt gctgcgacg 
tgtttgtgt ctaacacat tgtggaaac tttgttctt 
gttgggtac agcacagga catattgat aggtttaac 
atgcagtca ggtatttgg cgcgacagt tacggcgcc 
cccaaagcc cacgatgca tcggttaga ggagtaaca 
accgatcct ttaaatcag atgacaata agtggaagc 
agtgattgt aaaatcaaa ttttggaag tttaaaaat 
aaaggagcc aatccattg acggtctta acactggac 
gagccaatc agttttttt cggtcgcat tcagaaagc 
tcaatgttg gccgttgcc ctcgaagac tttgacgtt 
tacgtagta gatatcgac acgagacga atcgtgcga 
aaatttgtg gggcacacg gcacaaata accgacgca 
acatttagc cctgactct cgctggttg gtgacttca 
tcaatggat tgttccata cgtacgtgg gacatacct 
tcgtgtcat ttagtggac gagttcagt acagaggtg 
gcctgcgtt tcattggac atttcgccg accggggaa 
gtcctggct acagcacat gtcgattac ttggggatt 
tttctgtgg acaaatcga actttatac tcaaaagta 
tcactgaag gctttaaca ccaagcgtt caaccgcca 
ctggtcgct ttccctgaa tgtctcaca gagcaccac 
gaagagaaa agcgaggtg gaagaatcc gacgatctg 
gaattcata tcgcccgaa caaattagc aacgacttg 
gttaccttg tcaggcttt gctgcctca aggtggcag 
aatttgttg aatttggat gtgatcaag aagaggaat 
aaacccaaa tccccgcct aaggcacct aaagccgca 
ccgtttttc ctaccgact gtttcatct ttaactttc 
caattcgat ttgtcaaaa cccgaatct gagagtagt 
aaaatattg acgccaagc actttattg aatctaacc 
gaatttggg aaattattg gataaaacg agacaagac 
gacgatttt agccacgtt attgagaag ctaaagtcg 
tttggtccg tctatgatt gactttgaa ataaagtct 
ttggcccct gaaggtgga ggctcagtt gaagtgatg 
ctacagttt ctgaagagt gtcgaattt atgttgaaa 
tcgaacaaa gactttgag ttagcggag gcctacctt 
ggcgtcttt ctaaaatca cacgggaca gtggttgca 
gccgaggag actttgagg aattattta accaatatc 
cagagttgt cacaatgtt gtatggaat aggttgcaa 
gataaattg ttgtacaat atttgtgtt gttcaaaac 
ttaaaaatg atgtaa
    mRNA sequence
    • >TC000478 RA
      tccacgtgt gtgtgttta ctgatttaa atttaccga 
aatctgaaa tgcctaaag gcagcaaaa tatttttgc 
cctgtcgga gcttagggt acgtcagca accacatcc 
cgcttcagg tccgctaca taaaaagtc gcaaagaaa 
atctaatag tgacatgtg tggggaaat cgtttcaca 
cgtacggaa taacacact ttggcctgt taagtgtcg 
gtggtttgc accccgaag acataactg cgatgaccg 
ctgatgcct ttcatgtgt acacagcgt gcaataatg 
agatttatg cctggagaa gaggcactg agttgaaac 
atgtctata gaggacaca agaaaccgg ttcatttga 
tgatgcctt ttggggcac atttgattt caattgatg 
aggccagta gtgttaaac tgtgggata ttaaggatg 
agagtgttt ttcttgaac tcacgttta gtaacaaga 
cattccata tcaccacaa tctgccacc ccagcactt 
atattaata agattttgt tgggtagtg aacagggag 
agatgcagt tatggaatg tcaataatt tgaaaatga 
tttattcct tcaaggggt ggaacagcc ccattactt 
gcttggaac aagcgcctg caattgatg tggttgctg 
ttggtttgg cttgcggta aaatcaaat tacacaatt 
taaaattcg acgagacca taatggaat ttatgcaag 
attggggcg tcgtcactt cgatatcat ttaggaccg 
atggtagtc ccataatgg caacaggaa gtgtaatag 
gccatatag tattttggg atttggaag aaagacgcg 
tcgcgagcc aactacttt ctgcccacg acggcagcg 
ttacaggaa tgatttgtc tcccgagtg aacctttaa 
tcctaactt cgtctccag acaatactt taaaattat 
ggatctttg acaggtcag acggtggtg ccagacttt 
taagaataa gagaaggcc attcagcac cgccgtctc 
accttagat ttcacggtg ctaacggac acaacattt 
taagttgcg ctggagatt caacactga gaatattta 
atacgcaaa ctgagcaat tcaataaaa gtctaggaa 
aagcgtcgt acaacagaa aagcgtcaa agaagcgcg 
ggagaaccg ccgaagacc cgctaatta tgccaccaa 
tcacacaat ttacttccg aaattacaa gggagaaag 
aatgggata atatcgcag cggtacatt tggggatac 
ccatggtta ccacatggt cttacgata aggtgaaga 
tgggcgaat tgaaattgc ttcctgaac ggtttcaca 
agaaaaaac tcgtttcta gtgggtttc acgacgttg 
ctgcgacgt gtttgtgtc taacacatt gtggaaact 
ttgttcttg ttgggtaca gcacaggac atattgata 
ggtttaaca tgcagtcag gtatttggc gcgacagtt 
acggcgccc ccaaagccc acgatgcat cggttagag 
gagtaacaa ccgatcctt taaatcaga tgacaataa 
gtggaagca gtgattgta aaatcaaat tttggaagt 
ttaaaaata aaggagcca atccattga cggtcttaa 
cactggacg agccaatca gtttttttc ggtcgcatt 
cagaaagct caatgttgg ccgttgccc tcgaagact 
ttgacgttt acgtagtag atatcgaca cgagacgaa 
tcgtgcgaa aatttgtgg ggcacacgg cacaaataa 
ccgacgcaa catttagcc ctgactctc gctggttgg 
tgacttcat caatggatt gttccatac gtacgtggg 
acatacctt cgtgtcatt tagtggacg agttcagta 
cagaggtgg cctgcgttt cattggaca tttcgccga 
ccggggaag tcctggcta cagcacatg tcgattact 
tggggattt ttctgtgga caaatcgaa ctttatact 
caaaagtat cactgaagg ctttaacac caagcgttc 
aaccgccac tggtcgctt tccctgaat gtctcacag 
agcaccacg aagagaaaa gcgaggtgg aagaatccg 
acgatctgg aattcatat cgcccgaac aaattagca 
acgacttgg ttaccttgt caggctttg ctgcctcaa 
ggtggcaga atttgttga atttggatg tgatcaaga 
agaggaata aacccaaat ccccgccta aggcaccta 
aagccgcac cgtttttcc taccgactg tttcatctt 
taactttcc aattcgatt tgtcaaaac ccgaatctg 
agagtagta aaatattga cgccaagca ctttattga 
atctaaccg aatttggga aattattgg ataaaacga 
gacaagacg acgatttta gccacgtta ttgagaagc 
taaagtcgt ttggtccgt ctatgattg actttgaaa 
taaagtctt tggcccctg aaggtggag gctcagttg 
aagtgatgc tacagtttc tgaagagtg tcgaattta 
tgttgaaat cgaacaaag actttgagt tagcggagg 
cctaccttg gcgtctttc taaaatcac acgggacag 
tggttgcag ccgaggaga ctttgagga attatttaa 
ccaatatcc agagttgtc acaatgttg tatggaata 
ggttgcaag ataaattgt tgtacaata tttgtgttg 
ttcaaaact taaaaatga tgtaaataa aacgaaaat 
ttttaattg tttcgtttc ttaccctgt ttgaggcac 
aaattacac tagaataaa caaaaattg gggccatgt 
ctttgaatt ttgttaacg cgcggccgg taatatcgg 
gcgcaatat tatcatcat agcacgaaa ttcgtaaac 
aagaataaa ccaaaggga ttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY