| General information | |||
| TC000478 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10820855..10824197 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000478 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03027 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000478 RA
MPKGSKIFL PCRSLGYVS NHIPLQVRY IKSRKENLI VTCVGKSFH TYGITHFGL LSVGGLHPE DITAMTADA FHVYTACNN EIYAWRRGT ELKHVYRGH KKPVHLMMP FGAHLISID EASSVKLWD IKDESVFLE LTFSNKTFH ITTICHPST YINKILLGS EQGEMQLWN VNNLKMIYS FKGWNSPIT CLEQAPAID VVAVGLACG KIKLHNLKF DETIMEFMQ DWGVVTSIS FRTDGSPIM ATGSVIGHI VFWDLEERR VASQLLSAH DGSVTGMIC LPSEPLILT SSPDNTLKL WIFDRSDGG ARLLRIREG HSAPPSHLR FHGANGHNI LSCAGDSTL RIFNTQTEQ FNKSLGKAS YNRKASKKR GRTAEDPLI MPPITQFTS EITREKEWD NIAAVHLGI PMVTTWSYD KVKMGELKL LPERFHKKK LVSSGFHDV AATCLCLTH CGNFVLVGY STGHIDRFN MQSGIWRDS YGAPKAHDA SVRGVTTDP LNQMTISGS SDCKIKFWK FKNKGANPL TVLTLDEPI SFFRSHSES SMLAVALED FDVYVVDID TRRIVRKFV GHTAQITDA TFSPDSRWL VTSSMDCSI RTWDIPSCH LVDEFSTEV ACVSLDISP TGEVLATAH VDYLGIFLW TNRTLYSKV SLKALTPSV QPPLVAFPE CLTEHHEEK SEVEESDDL EFISPEQIS NDLVTLSGF AASRWQNLL NLDVIKKRN KPKSPPKAP KAAPFFLPT VSSLTFQFD LSKPESESS KILTPSTLL NLTEFGKLL DKTRQDDDF SHVIEKLKS FGPSMIDFE IKSLAPEGG GSVEVMLQF LKSVEFMLK SNKDFELAE AYLGVFLKS HGTVVAAEE TLRNYLTNI QSCHNVVWN RLQDKLLYN ICVVQNLKM M
- >TC000478 RA
atgcctaaa ggcagcaaa atatttttg ccctgtcgg agcttaggg tacgtcagc aaccacatc ccgcttcag gtccgctac ataaaaagt cgcaaagaa aatctaata gtgacatgt gtggggaaa tcgtttcac acgtacgga ataacacac tttggcctg ttaagtgtc ggtggtttg caccccgaa gacataact gcgatgacc gctgatgcc tttcatgtg tacacagcg tgcaataat gagatttat gcctggaga agaggcact gagttgaaa catgtctat agaggacac aagaaaccg gttcatttg atgatgcct tttggggca catttgatt tcaattgat gaggccagt agtgttaaa ctgtgggat attaaggat gagagtgtt tttcttgaa ctcacgttt agtaacaag acattccat atcaccaca atctgccac cccagcact tatattaat aagattttg ttgggtagt gaacaggga gagatgcag ttatggaat gtcaataat ttgaaaatg atttattcc ttcaagggg tggaacagc cccattact tgcttggaa caagcgcct gcaattgat gtggttgct gttggtttg gcttgcggt aaaatcaaa ttacacaat ttaaaattc gacgagacc ataatggaa tttatgcaa gattggggc gtcgtcact tcgatatca tttaggacc gatggtagt cccataatg gcaacagga agtgtaata ggccatata gtattttgg gatttggaa gaaagacgc gtcgcgagc caactactt tctgcccac gacggcagc gttacagga atgatttgt ctcccgagt gaaccttta atcctaact tcgtctcca gacaatact ttaaaatta tggatcttt gacaggtca gacggtggt gccagactt ttaagaata agagaaggc cattcagca ccgccgtct caccttaga tttcacggt gctaacgga cacaacatt ttaagttgc gctggagat tcaacactg agaatattt aatacgcaa actgagcaa ttcaataaa agtctagga aaagcgtcg tacaacaga aaagcgtca aagaagcgc gggagaacc gccgaagac ccgctaatt atgccacca atcacacaa tttacttcc gaaattaca agggagaaa gaatgggat aatatcgca gcggtacat ttggggata cccatggtt accacatgg tcttacgat aaggtgaag atgggcgaa ttgaaattg cttcctgaa cggtttcac aagaaaaaa ctcgtttct agtgggttt cacgacgtt gctgcgacg tgtttgtgt ctaacacat tgtggaaac tttgttctt gttgggtac agcacagga catattgat aggtttaac atgcagtca ggtatttgg cgcgacagt tacggcgcc cccaaagcc cacgatgca tcggttaga ggagtaaca accgatcct ttaaatcag atgacaata agtggaagc agtgattgt aaaatcaaa ttttggaag tttaaaaat aaaggagcc aatccattg acggtctta acactggac gagccaatc agttttttt cggtcgcat tcagaaagc tcaatgttg gccgttgcc ctcgaagac tttgacgtt tacgtagta gatatcgac acgagacga atcgtgcga aaatttgtg gggcacacg gcacaaata accgacgca acatttagc cctgactct cgctggttg gtgacttca tcaatggat tgttccata cgtacgtgg gacatacct tcgtgtcat ttagtggac gagttcagt acagaggtg gcctgcgtt tcattggac atttcgccg accggggaa gtcctggct acagcacat gtcgattac ttggggatt tttctgtgg acaaatcga actttatac tcaaaagta tcactgaag gctttaaca ccaagcgtt caaccgcca ctggtcgct ttccctgaa tgtctcaca gagcaccac gaagagaaa agcgaggtg gaagaatcc gacgatctg gaattcata tcgcccgaa caaattagc aacgacttg gttaccttg tcaggcttt gctgcctca aggtggcag aatttgttg aatttggat gtgatcaag aagaggaat aaacccaaa tccccgcct aaggcacct aaagccgca ccgtttttc ctaccgact gtttcatct ttaactttc caattcgat ttgtcaaaa cccgaatct gagagtagt aaaatattg acgccaagc actttattg aatctaacc gaatttggg aaattattg gataaaacg agacaagac gacgatttt agccacgtt attgagaag ctaaagtcg tttggtccg tctatgatt gactttgaa ataaagtct ttggcccct gaaggtgga ggctcagtt gaagtgatg ctacagttt ctgaagagt gtcgaattt atgttgaaa tcgaacaaa gactttgag ttagcggag gcctacctt ggcgtcttt ctaaaatca cacgggaca gtggttgca gccgaggag actttgagg aattattta accaatatc cagagttgt cacaatgtt gtatggaat aggttgcaa gataaattg ttgtacaat atttgtgtt gttcaaaac ttaaaaatg atgtaa
- >TC000478 RA
tccacgtgt gtgtgttta ctgatttaa atttaccga aatctgaaa tgcctaaag gcagcaaaa tatttttgc cctgtcgga gcttagggt acgtcagca accacatcc cgcttcagg tccgctaca taaaaagtc gcaaagaaa atctaatag tgacatgtg tggggaaat cgtttcaca cgtacggaa taacacact ttggcctgt taagtgtcg gtggtttgc accccgaag acataactg cgatgaccg ctgatgcct ttcatgtgt acacagcgt gcaataatg agatttatg cctggagaa gaggcactg agttgaaac atgtctata gaggacaca agaaaccgg ttcatttga tgatgcctt ttggggcac atttgattt caattgatg aggccagta gtgttaaac tgtgggata ttaaggatg agagtgttt ttcttgaac tcacgttta gtaacaaga cattccata tcaccacaa tctgccacc ccagcactt atattaata agattttgt tgggtagtg aacagggag agatgcagt tatggaatg tcaataatt tgaaaatga tttattcct tcaaggggt ggaacagcc ccattactt gcttggaac aagcgcctg caattgatg tggttgctg ttggtttgg cttgcggta aaatcaaat tacacaatt taaaattcg acgagacca taatggaat ttatgcaag attggggcg tcgtcactt cgatatcat ttaggaccg atggtagtc ccataatgg caacaggaa gtgtaatag gccatatag tattttggg atttggaag aaagacgcg tcgcgagcc aactacttt ctgcccacg acggcagcg ttacaggaa tgatttgtc tcccgagtg aacctttaa tcctaactt cgtctccag acaatactt taaaattat ggatctttg acaggtcag acggtggtg ccagacttt taagaataa gagaaggcc attcagcac cgccgtctc accttagat ttcacggtg ctaacggac acaacattt taagttgcg ctggagatt caacactga gaatattta atacgcaaa ctgagcaat tcaataaaa gtctaggaa aagcgtcgt acaacagaa aagcgtcaa agaagcgcg ggagaaccg ccgaagacc cgctaatta tgccaccaa tcacacaat ttacttccg aaattacaa gggagaaag aatgggata atatcgcag cggtacatt tggggatac ccatggtta ccacatggt cttacgata aggtgaaga tgggcgaat tgaaattgc ttcctgaac ggtttcaca agaaaaaac tcgtttcta gtgggtttc acgacgttg ctgcgacgt gtttgtgtc taacacatt gtggaaact ttgttcttg ttgggtaca gcacaggac atattgata ggtttaaca tgcagtcag gtatttggc gcgacagtt acggcgccc ccaaagccc acgatgcat cggttagag gagtaacaa ccgatcctt taaatcaga tgacaataa gtggaagca gtgattgta aaatcaaat tttggaagt ttaaaaata aaggagcca atccattga cggtcttaa cactggacg agccaatca gtttttttc ggtcgcatt cagaaagct caatgttgg ccgttgccc tcgaagact ttgacgttt acgtagtag atatcgaca cgagacgaa tcgtgcgaa aatttgtgg ggcacacgg cacaaataa ccgacgcaa catttagcc ctgactctc gctggttgg tgacttcat caatggatt gttccatac gtacgtggg acatacctt cgtgtcatt tagtggacg agttcagta cagaggtgg cctgcgttt cattggaca tttcgccga ccggggaag tcctggcta cagcacatg tcgattact tggggattt ttctgtgga caaatcgaa ctttatact caaaagtat cactgaagg ctttaacac caagcgttc aaccgccac tggtcgctt tccctgaat gtctcacag agcaccacg aagagaaaa gcgaggtgg aagaatccg acgatctgg aattcatat cgcccgaac aaattagca acgacttgg ttaccttgt caggctttg ctgcctcaa ggtggcaga atttgttga atttggatg tgatcaaga agaggaata aacccaaat ccccgccta aggcaccta aagccgcac cgtttttcc taccgactg tttcatctt taactttcc aattcgatt tgtcaaaac ccgaatctg agagtagta aaatattga cgccaagca ctttattga atctaaccg aatttggga aattattgg ataaaacga gacaagacg acgatttta gccacgtta ttgagaagc taaagtcgt ttggtccgt ctatgattg actttgaaa taaagtctt tggcccctg aaggtggag gctcagttg aagtgatgc tacagtttc tgaagagtg tcgaattta tgttgaaat cgaacaaag actttgagt tagcggagg cctaccttg gcgtctttc taaaatcac acgggacag tggttgcag ccgaggaga ctttgagga attatttaa ccaatatcc agagttgtc acaatgttg tatggaata ggttgcaag ataaattgt tgtacaata tttgtgttg ttcaaaact taaaaatga tgtaaataa aacgaaaat ttttaattg tttcgtttc ttaccctgt ttgaggcac aaattacac tagaataaa caaaaattg gggccatgt ctttgaatt ttgttaacg cgcggccgg taatatcgg gcgcaatat tatcatcat agcacgaaa ttcgtaaac aagaataaa ccaaaggga ttatt
