Status
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Gene information
General information
TC000457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11051843..11055920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000457
    iBeetle screen iB_03025

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000457 RA
      MESSKELLE KLLTDEVLD SLIQDTACL QTLKPLLEV 
      KHERIIGGQ CEKITLSLN SLYDSDVDV DLAAEILKR 
      ALYVTRDNT LLSQTQSFC LSKLQSSRS LDELYYCCN 
      LLNVLFEYY KSEKLALME WKSDEFSNG ILNTLVASS 
      KPLFTFVTI YLLPNYFTV CSRNNKNLF LENAFKFIR 
      DNKRLDFLC VLWAHFIPV DLTSNNFNV LKVDEFWKL 
      VIESLSTQS QKQAIYLLS KSTEIAFKP ENIPHIPNY 
      LKVENESEI EKIWRNYFT LLDVSNEKQ LHFIEPSLH 
      MLGSIKHLQ IEWRLCLYK ILLNHSQTS VVYSVANFI 
      IENENVWQD FSKILENLL HAVNKHECN EQSKSVFRK 
      IGIYCSKLN EFQFQQFLV DCMNPAWTP SALWGLFSN 
      IFTDEMNFQ ISFELFSKI IKKIKVLPH KFIRESCTR 
      IVFQHFIRN HTLESVDDV FKISILLYD FNEALFRDF 
      ITKNAQNIL NFKDELCDK FQACDEWVE IKVRIFQIL 
      KIKIPVFPV TPTQQNDLI FGFLLEDVE TSVLEKYVI 
      SRIESVLET DDISALLNI LLKFTTPKL CENAEIILS 
      RQQYNPSQK IIALGILKQ NRNSITFCK NLGKKSDPL 
      TTSIVKILL NSLHDNSSE ITDCINFFE AVFDKQSNA 
      TITILENIS VILKFIDRD FVFDFFILC LNEFLNLHK 
      SGNFRTTAS VFLKQIFEA QFFENGRFC NAIFDFVKK 
      LLPLANTAN SVTYCLAKM IRSVCQQNP QMGLIFVPV 
      IAELILHGI VISKNERVE YNVCQEIYS KLGKIENNP 
      NFEEMSVRL TALDTMLDL INNPSLETE VVVSQFVSV 
      LIEKYNEYF SKRYFPDSL IHFQKLRIM QTLLLLHEH 
      IKAEKTPLV NLLIKSFLL ESHQPSVKH LMQWLLISL 
      LQTDSDVFI QTINNNLKS VNNNRPSTT SGFIPILYN 
      LVTLQREAQ WEQVTEMLL PYTMGPQFK LRIFSQVAL 
      VKLYEKAKD KRLDSFTEK YKILYNSLK QVLAATNSV 
      YDSDTIQHE FLDFINLSQ CDCSTIFYL IPRKNAVSA 
      TEWENLKTV SDWEHFKFI SFNHSDNNI QETYAPITT 
      HTIASDNIQ KKITPWKDF LSTSPSQEN NFILVTTLI 
      EKSTNLGGL SRTCEVFGI KQIVMRTAK IISDKEFKS 
      LSMSSENSV EILEVKPED LASFILNMK AQGYSVVGA 
      EQTSESVKL DAFRFDKKT VLVLGNEKE GIPPDLIPL 
      LDHCIEIPQ FGLVRSLNV HVAGAIVIW EYCKQHRL
    CDS sequence
    • >TC000457 RA
      atggaatca tccaaggaa ctgctagaa aagctctta 
      actgacgaa gtcttggac agtttaatt caagacacg 
      gcctgtttg caaactttg aaacccctt cttgaagtc 
      aagcacgaa cggatcatt gggggccag tgtgagaaa 
      atcaccttg tctttgaac tccctttac gacagtgat 
      gtagatgta gatttggca gcagaaatt ttaaaacgt 
      gctttatac gttacgcga gataatact cttttgtcg 
      caaacccag tctttctgc ttaagcaaa ttgcagtca 
      agtcgaagc cttgacgaa ctttattac tgttgcaat 
      ttgttaaac gtacttttt gagtattat aagagcgaa 
      aaactggct ctaatggaa tggaaaagt gacgagttt 
      tccaatggg attttaaat actttggta gcttcatca 
      aaacctttg ttcactttt gttacgatt tatttacta 
      cctaactac tttactgtg tgtagtcgg aacaataag 
      aatttgttt ttagaaaat gcgtttaaa tttatcagg 
      gataataaa agactggac tttttgtgt gtattgtgg 
      gcccatttc attcctgtt gatttaact agcaataat 
      ttcaatgtt ttgaaagtt gatgagttt tggaaactc 
      gtaatcgag tctttatca acccagtca caaaaacaa 
      gcgatttat ttgttatcc aaaagtacc gaaattgcg 
      ttcaaaccc gaaaatatc ccccacatt cccaattac 
      ctaaaagtc gaaaatgag tctgaaatt gaaaaaatc 
      tggcgcaac tatttcact ctcctcgat gtttcaaac 
      gagaaacag ttgcatttt atcgagcct tctttgcac 
      atgttgggg tccattaaa catttgcaa attgaatgg 
      agattatgc ttgtacaaa attcttcta aaccactca 
      caaacaagt gttgtttac agtgtagct aattttatc 
      atagaaaac gaaaatgtt tggcaagat ttttcaaaa 
      atattagaa aatttatta cacgctgtc aacaaacac 
      gaatgtaat gaacagtct aaaagcgtg tttcgaaaa 
      attggaatt tattgctca aaacttaac gaatttcaa 
      tttcaacaa tttttagtt gattgtatg aacccggct 
      tggaccccc tcggctctt tggggttta tttagcaat 
      attttcacc gacgaaatg aatttccaa atttcgttt 
      gaactgttt tctaagata attaaaaaa attaaagtg 
      cttccacac aaatttata agggaatcg tgcacacga 
      attgtcttt caacatttt attagaaat cacactctc 
      gaatcggtc gacgatgtt ttcaaaatt tcgatttta 
      ctttacgat tttaacgaa gctttattt cgggatttt 
      attacaaaa aatgcccaa aatattctt aattttaag 
      gatgaactt tgtgataaa tttcaagcc tgtgatgaa 
      tgggttgaa attaaagtc agaattttc caaatttta 
      aaaatcaaa attccagtg tttcctgtt actccgacc 
      cagcaaaat gatttaatt tttggtttc ctgcttgaa 
      gatgttgaa acgtccgta ttagagaaa tatgtgata 
      agtcgaata gaatcagtg cttgaaact gacgatatc 
      tcggctctg ttaaacatt ttgttaaaa ttcacgaca 
      ccaaaattg tgtgaaaat gccgaaatt attctgtca 
      cggcaacaa tacaacccc agtcaaaaa ataatagca 
      cttggaata cttaaacaa aatcgcaac tcaatcact 
      ttttgcaag aatcttggc aaaaaatct gacccactt 
      actacatcc attgtaaaa attttactc aattctttg 
      cacgataat tccagtgaa attaccgat tgtattaat 
      tttttcgaa gctgtgttt gataagcaa agtaacgca 
      acaattaca atcctcgag aacatatcc gttatttta 
      aaattcatt gaccgcgac tttgtgttc gattttttc 
      atactgtgt ctgaacgaa tttttgaat ttacacaaa 
      agtggtaat ttccgaacc acagcaagc gtgtttttg 
      aaacaaata tttgaagca cagtttttc gaaaatggt 
      aggttttgt aacgcaatt ttcgatttc gtcaaaaaa 
      ttgttgcca cttgcaaac accgcgaac tctgttact 
      tattgtctc gcaaaaatg atccgatct gtgtgccaa 
      caaaacccc caaatgggc ctaatattt gtccctgta 
      atcgctgaa ctaatctta catggaatt gtgattagt 
      aaaaatgaa cgggttgaa tataatgtg tgtcaggag 
      atatactcc aagttgggg aaaatcgag aataatcca 
      aattttgaa gaaatgtca gtacggttg actgcgtta 
      gatacgatg ctagattta attaacaat cccagtttg 
      gagactgaa gtagtggtc agtcaattc gtttcagtg 
      ttgattgaa aaatacaac gaatatttt tcaaaaaga 
      tatttcccc gattcgctg atccatttc caaaaatta 
      cgaataatg caaaccttg ttactgtta catgaacac 
      ataaaagcc gaaaaaaca cctttggta aatctttta 
      attaaaagt tttttactg gaaagtcac caaccatcg 
      gtgaaacat ttgatgcaa tggcttttg ataagttta 
      ctacaaacc gatagtgac gtttttatt caaacaatt 
      aacaacaac cttaaatca gttaataat aatcgaccg 
      tcaacaact tctggcttt atcccaata ctttataac 
      ttagtaact ctccaacgt gaggcacag tgggaacaa 
      gttactgaa atgcttttg ccttacacc atgggacca 
      cagttcaag cttcgcatt ttttcccaa gtcgcttta 
      gtaaagctg tacgaaaaa gcgaaagat aaaagacta 
      gacagtttt accgaaaaa tataaaatt ctgtacaat 
      tcgctcaaa caagtgctc gcagctacg aattccgtt 
      tacgactct gacacgata caacacgaa tttctcgat 
      tttattaat ttgtcacag tgcgactgt tcgacgata 
      ttttacttg attccgagg aaaaacgca gtttcagcc 
      acagaatgg gaaaattta aaaaccgta tccgactgg 
      gaacacttc aaatttatt tcattcaac cattctgac 
      aacaatatt caagaaact tacgcaccg ataacaacc 
      cacacgatt gctagtgac aatattcaa aagaaaatt 
      acaccgtgg aaagatttt ttaagtacc agtccatcg 
      caggaaaat aatttcata ttagtgaca acactaatt 
      gagaaatcg acaaatctg ggcggttta tcccgaact 
      tgtgaggtt tttggcatc aaacaaatc gtcatgagg 
      acggctaaa atcatctct gataaagaa ttcaaaagt 
      ttgagcatg tcgtcggag aattcggtc gagattttg 
      gaagtgaag cctgaagat ttggcgagt tttattctc 
      aacatgaag gcacaaggg tacagtgtt gtcggtgcc 
      gaacagact tcggaaagt gtgaagttg gatgcgttc 
      cgatttgac aaaaaaaca gtccttgtg ctaggaaat 
      gaaaaagaa gggattccg cctgattta atcccactt 
      cttgaccat tgtatcgaa ataccgcag ttcggactc 
      gtccgttct ttgaacgtc cacgtcgct ggggccata 
      gtcatttgg gagtattgc aaacaacac agattgtaa
    mRNA sequence
    • >TC000457 RA
      accaagaaa atttgtgtt tgaaatgtg ttctttcaa 
      cgctaatta aaatggaat catccaagg aactgctag 
      aaaagctct taactgacg aagtcttgg acagtttaa 
      ttcaagaca cggcctgtt tgcaaactt tgaaacccc 
      ttcttgaag tcaagcacg aacggatca ttgggggcc 
      agtgtgaga aaatcacct tgtctttga actcccttt 
      acgacagtg atgtagatg tagatttgg cagcagaaa 
      ttttaaaac gtgctttat acgttacgc gagataata 
      ctcttttgt cgcaaaccc agtctttct gcttaagca 
      aattgcagt caagtcgaa gccttgacg aactttatt 
      actgttgca atttgttaa acgtacttt ttgagtatt 
      ataagagcg aaaaactgg ctctaatgg aatggaaaa 
      gtgacgagt tttccaatg ggattttaa atactttgg 
      tagcttcat caaaacctt tgttcactt ttgttacga 
      tttatttac tacctaact actttactg tgtgtagtc 
      ggaacaata agaatttgt ttttagaaa atgcgttta 
      aatttatca gggataata aaagactgg actttttgt 
      gtgtattgt gggcccatt tcattcctg ttgatttaa 
      ctagcaata atttcaatg ttttgaaag ttgatgagt 
      tttggaaac tcgtaatcg agtctttat caacccagt 
      cacaaaaac aagcgattt atttgttat ccaaaagta 
      ccgaaattg cgttcaaac ccgaaaata tcccccaca 
      ttcccaatt acctaaaag tcgaaaatg agtctgaaa 
      ttgaaaaaa tctggcgca actatttca ctctcctcg 
      atgtttcaa acgagaaac agttgcatt ttatcgagc 
      cttctttgc acatgttgg ggtccatta aacatttgc 
      aaattgaat ggagattat gcttgtaca aaattcttc 
      taaaccact cacaaacaa gtgttgttt acagtgtag 
      ctaatttta tcatagaaa acgaaaatg tttggcaag 
      atttttcaa aaatattag aaaatttat tacacgctg 
      tcaacaaac acgaatgta atgaacagt ctaaaagcg 
      tgtttcgaa aaattggaa tttattgct caaaactta 
      acgaatttc aatttcaac aatttttag ttgattgta 
      tgaacccgg cttggaccc cctcggctc tttggggtt 
      tatttagca atattttca ccgacgaaa tgaatttcc 
      aaatttcgt ttgaactgt tttctaaga taattaaaa 
      aaattaaag tgcttccac acaaattta taagggaat 
      cgtgcacac gaattgtct ttcaacatt ttattagaa 
      atcacactc tcgaatcgg tcgacgatg ttttcaaaa 
      tttcgattt tactttacg attttaacg aagctttat 
      ttcgggatt ttattacaa aaaatgccc aaaatattc 
      ttaatttta aggatgaac tttgtgata aatttcaag 
      cctgtgatg aatgggttg aaattaaag tcagaattt 
      tccaaattt taaaaatca aaattccag tgtttcctg 
      ttactccga cccagcaaa atgatttaa tttttggtt 
      tcctgcttg aagatgttg aaacgtccg tattagaga 
      aatatgtga taagtcgaa tagaatcag tgcttgaaa 
      ctgacgata tctcggctc tgttaaaca ttttgttaa 
      aattcacga caccaaaat tgtgtgaaa atgccgaaa 
      ttattctgt cacggcaac aatacaacc ccagtcaaa 
      aaataatag cacttggaa tacttaaac aaaatcgca 
      actcaatca ctttttgca agaatcttg gcaaaaaat 
      ctgacccac ttactacat ccattgtaa aaattttac 
      tcaattctt tgcacgata attccagtg aaattaccg 
      attgtatta attttttcg aagctgtgt ttgataagc 
      aaagtaacg caacaatta caatcctcg agaacatat 
      ccgttattt taaaattca ttgaccgcg actttgtgt 
      tcgattttt tcatactgt gtctgaacg aatttttga 
      atttacaca aaagtggta atttccgaa ccacagcaa 
      gcgtgtttt tgaaacaaa tatttgaag cacagtttt 
      tcgaaaatg gtaggtttt gtaacgcaa ttttcgatt 
      tcgtcaaaa aattgttgc cacttgcaa acaccgcga 
      actctgtta cttattgtc tcgcaaaaa tgatccgat 
      ctgtgtgcc aacaaaacc cccaaatgg gcctaatat 
      ttgtccctg taatcgctg aactaatct tacatggaa 
      ttgtgatta gtaaaaatg aacgggttg aatataatg 
      tgtgtcagg agatatact ccaagttgg ggaaaatcg 
      agaataatc caaattttg aagaaatgt cagtacggt 
      tgactgcgt tagatacga tgctagatt taattaaca 
      atcccagtt tggagactg aagtagtgg tcagtcaat 
      tcgtttcag tgttgattg aaaaataca acgaatatt 
      tttcaaaaa gatatttcc ccgattcgc tgatccatt 
      tccaaaaat tacgaataa tgcaaacct tgttactgt 
      tacatgaac acataaaag ccgaaaaaa cacctttgg 
      taaatcttt taattaaaa gttttttac tggaaagtc 
      accaaccat cggtgaaac atttgatgc aatggcttt 
      tgataagtt tactacaaa ccgatagtg acgttttta 
      ttcaaacaa ttaacaaca accttaaat cagttaata 
      ataatcgac cgtcaacaa cttctggct ttatcccaa 
      tactttata acttagtaa ctctccaac gtgaggcac 
      agtgggaac aagttactg aaatgcttt tgccttaca 
      ccatgggac cacagttca agcttcgca ttttttccc 
      aagtcgctt tagtaaagc tgtacgaaa aagcgaaag 
      ataaaagac tagacagtt ttaccgaaa aatataaaa 
      ttctgtaca attcgctca aacaagtgc tcgcagcta 
      cgaattccg tttacgact ctgacacga tacaacacg 
      aatttctcg attttatta atttgtcac agtgcgact 
      gttcgacga tattttact tgattccga ggaaaaacg 
      cagtttcag ccacagaat gggaaaatt taaaaaccg 
      tatccgact gggaacact tcaaattta tttcattca 
      accattctg acaacaata ttcaagaaa cttacgcac 
      cgataacaa cccacacga ttgctagtg acaatattc 
      aaaagaaaa ttacaccgt ggaaagatt ttttaagta 
      ccagtccat cgcaggaaa ataatttca tattagtga 
      caacactaa ttgagaaat cgacaaatc tgggcggtt 
      tatcccgaa cttgtgagg tttttggca tcaaacaaa 
      tcgtcatga ggacggcta aaatcatct ctgataaag 
      aattcaaaa gtttgagca tgtcgtcgg agaattcgg 
      tcgagattt tggaagtga agcctgaag atttggcga 
      gttttattc tcaacatga aggcacaag ggtacagtg 
      ttgtcggtg ccgaacaga cttcggaaa gtgtgaagt 
      tggatgcgt tccgatttg acaaaaaaa cagtccttg 
      tgctaggaa atgaaaaag aagggattc cgcctgatt 
      taatcccac ttcttgacc attgtatcg aaataccgc 
      agttcggac tcgtccgtt ctttgaacg tccacgtcg 
      ctggggcca tagtcattt gggagtatt gcaaacaac 
      acagattgt aacatattt tattaaaga ataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY