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Gene information
General information
TC000456 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11062504..11064950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000456
    iBeetle screen iB_03024

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000456 RA
      MMNPQPYYG IQSTAGAVP VRNEKGEIS MQKVKVHRY 
      VSGKKPDYA QDARSSSDS DDDDFLERR NQPQQPPTP 
      EPSRSDDEL NDPRLRRLK IAEVETEVR PERRRHVVE 
      PEVLESSDE ESEKEQINL PEHKHTLEA APDDDESEV 
      ELSDSEIEK RREMMKKRM MEKQEEEFM VKEEEKSES 
      SEESSEYEE YTDSEEETG PRLKPIFVR KKDRITIIE 
      KEKEVQKQK QQELEAVRN AEERRRQTL RLVEETIRK 
      EQQQKDKEN NEPNLNDVC TDDENDEIE YEAWKLREL 
      KRIKRDREE REAIEKERL EVERLRNMT EEERRLELR 
      LNPKQVTNK AIKGKYKFL QKYYHRGAF YLDKEDEVF 
      KRDFAQATL EDHFDKTIL PKVMQVKNF GRSGRTKYT 
      HLVDQDTTH FDSPWSSET SQNLKFHST QAGGVKQVF 
      EKPSLKKRK TQ
    CDS sequence
    • >TC000456 RA
      atgatgaac ccccagccc tattatggc atccagagt 
      actgcgggt gcggttccc gttcgcaac gaaaaaggc 
      gaaatttcg atgcaaaag gtgaaggtg caccgctac 
      gtgtctgga aagaagccc gattatgcc caagacgcg 
      cgttcgagc agcgacagc gacgacgac gatttcctg 
      gagcgtcgc aatcagccc caacagccc ccaacgccg 
      gagccttca cgcagcgat gacgaactg aatgacccc 
      cgtttgcgt cgtctgaaa atcgcagaa gttgaaact 
      gaagtgagg ccagaacgc cgccgtcac gttgtggag 
      ccagaagtg ttggaatcg tctgatgaa gaatccgaa 
      aaggaacaa attaattta cccgaacat aaacacaca 
      ctggaggcg gctcctgac gatgatgag tccgaagtg 
      gagcttagc gatagcgaa atcgagaaa cgcagagaa 
      atgatgaaa aaacgcatg atggaaaag caggaagag 
      gagtttatg gtgaaagaa gaagaaaag tcggagtcg 
      tcggaagaa tcgtcagag tatgaagag tacacagat 
      tcggaggag gagactggc cccagatta aaaccgatt 
      tttgtaaga aagaaagac aggatcacg atcatagaa 
      aaagaaaag gaggtacag aagcagaag cagcaagaa 
      ttggaagct gtgagaaat gcggaagaa aggcggcga 
      caaacgcta cgtcttgtt gaggaaaca atcaggaag 
      gaacagcaa cagaaagat aaagaaaat aacgaaccc 
      aaccttaac gacgtttgc acagacgat gaaaacgac 
      gaaattgaa tacgaagct tggaaacta agggagttg 
      aagcgaatc aaaagagat cgcgaagaa cgcgaagct 
      attgaaaaa gagcgactt gaagtcgaa cgattgagg 
      aacatgacg gaagaagag agaaggctt gagctgaga 
      ctaaacccc aaacaagtc acaaacaaa gccatcaag 
      gggaagtac aaattcttg cagaaatat taccaccgt 
      ggtgcgttc tatcttgat aaggaggac gaggttttc 
      aagagagat ttcgcacaa gcaactctg gaagatcac 
      ttcgataaa actattttg ccgaaagtt atgcaagtc 
      aaaaatttc ggacggtct ggacgaacg aagtacaca 
      catttggtg gatcaagac acaactcat ttcgattca 
      ccttggtca agtgaaacc tcgcaaaat ctcaaattt 
      cactcgaca caagcaggg ggcgtcaag caagtcttt 
      gaaaaaccc tcgttgaaa aaaaggaaa acgcagtag
    mRNA sequence
    • >TC000456 RA
      cgaaaatac ggtttcaaa gacggttct ttccatcac 
      acttaccac ttggtacaa gttgtgttg ccgtttgta 
      atccaatta ttgtaactc ataaaatgc acacaacac 
      agaaaatac acccgcaac cactctatt tgttcataa 
      tgttggaat attttggca caatcgcgt aaaaattat 
      aataacaca gcttcgccg cgtcttctt ccacgcttg 
      cgtttcgta gatttaact tgattcagc atcaacagg 
      tggtattgc caacccaac ggcaacagc taatttgca 
      tttcggttt cttacgttg gtggccctc gactttttt 
      tacccacag tctctgcgc tgagtttaa atcagttac 
      gaacaaaat ttttttttg gttcattta tcaaaaaaa 
      ttctcgaac cggttgatt ttattttta taattcatt 
      gattatttt tcaattcta tgcttgtca cttgtcaac 
      ctgtgttgt caactgtca aatgtaaag ttgtggcac 
      aaaaacaat tttcgttta ttttagaat taaaaacga 
      ctttcaata gtcgatttt cacaatgat gaaccccca 
      gccctatta tggcatcca gagtactgc gggtgcggt 
      tcccgttcg caacgaaaa aggcgaaat ttcgatgca 
      aaaggtgaa ggtgcaccg ctacgtgtc tggaaagaa 
      gcccgatta tgcccaaga cgcgcgttc gagcagcga 
      cagcgacga cgacgattt cctggagcg tcgcaatca 
      gccccaaca gcccccaac gccggagcc ttcacgcag 
      cgatgacga actgaatga cccccgttt gcgtcgtct 
      gaaaatcgc agaagttga aactgaagt gaggccaga 
      acgccgccg tcacgttgt ggagccaga agtgttgga 
      atcgtctga tgaagaatc cgaaaagga acaaattaa 
      tttacccga acataaaca cacactgga ggcggctcc 
      tgacgatga tgagtccga agtggagct tagcgatag 
      cgaaatcga gaaacgcag agaaatgat gaaaaaacg 
      catgatgga aaagcagga agaggagtt tatggtgaa 
      agaagaaga aaagtcgga gtcgtcgga agaatcgtc 
      agagtatga agagtacac agattcgga ggaggagac 
      tggccccag attaaaacc gatttttgt aagaaagaa 
      agacaggat cacgatcat agaaaaaga aaaggaggt 
      acagaagca gaagcagca agaattgga agctgtgag 
      aaatgcgga agaaaggcg gcgacaaac gctacgtct 
      tgttgagga aacaatcag gaaggaaca gcaacagaa 
      agataaaga aaataacga acccaacct taacgacgt 
      ttgcacaga cgatgaaaa cgacgaaat tgaatacga 
      agcttggaa actaaggga gttgaagcg aatcaaaag 
      agatcgcga agaacgcga agctattga aaaagagcg 
      acttgaagt cgaacgatt gaggaacat gacggaaga 
      agagagaag gcttgagct gagactaaa ccccaaaca 
      agtcacaaa caaagccat caaggggaa gtacaaatt 
      cttgcagaa atattacca ccgtggtgc gttctatct 
      tgataagga ggacgaggt tttcaagag agatttcgc 
      acaagcaac tctggaaga tcacttcga taaaactat 
      tttgccgaa agttatgca agtcaaaaa tttcggacg 
      gtctggacg aacgaagta cacacattt ggtggatca 
      agacacaac tcatttcga ttcaccttg gtcaagtga 
      aacctcgca aaatctcaa atttcactc gacacaagc 
      agggggcgt caagcaagt ctttgaaaa accctcgtt 
      gaaaaaaag gaaaacgca gtagtaagg tgtaataac 
      cactagact tagatgcaa aaaatatat atttttatt 
      catcaatta aagaacaat gtaataggg aaatcacac 
      tttcataca agcttcaag caatataca aagtaaatt 
      aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY