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Gene information
General information
TC000450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 5623208..5625177
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC000450
      iBeetle screen iB_03023
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC000450 RA
        MWNYLTAGG KRTFEDKAI QTIIQRKCG GDVSLKPDQ 
        EEQVFYKLL EYTPFRDIQ EESYKIVIL PRPKQENLP 
        SSIYLNLEN CLLIDDDAI SELCLELRE RIRKGYKQL 
        KTYNDMGSQ TDLHVQPHI LEDEKVASV VADCACFVL 
        LMDKSVPTI SSGADLDEE KSKFRETLA RKFKFLGTL 
        KNYIFSKLR KEKYNLDDS PPPAVSIVS YDQRGFEDA 
        EKALDSECS QTIKLDPRI LQALKTALN SKINDPKPN 
        ANLELLDIV ERTFARQMD IFEGLLKHI NSKSESDKV 
        KLQRKLRSS LKDRSLDLN YIINNLVTE LNANSNAQQ 
        ITIALNDDT PNPGDNPKI KKPSIECEC RQAGASGGA 
        VPRTQ
      CDS sequence
      • >TC000450 RA
        atgtggaat tacttgacg gctgggggt aaaagaacc 
        ttcgaggac aaagcaatt cagacgatt atccaacgc 
        aaatgcggt ggtgacgtg tctctgaaa cctgaccaa 
        gaggaacaa gtcttttac aagcttctg gagtacact 
        ccattcaga gacattcaa gaagaaagc tacaaaatc 
        gtcatttta ccgagacct aagcaggaa aatctgccg 
        tcatcgatt tacttaaac ttggagaac tgtcttttg 
        attgacgat gatgcgatc agtgagttg tgtctggaa 
        ctaagggag cgaattcgt aaagggtac aagcagttg 
        aaaacttac aatgacatg ggaagtcaa actgattta 
        cacgtccaa ccccatatt ttagaggat gagaaagtt 
        gcaagcgtt gttgcggac tgtgcttgt tttgtgtta 
        ttaatggac aaatctgta cccaccatc agctctgga 
        gcagacctg gatgaggag aaatcaaag tttagggaa 
        acccttgct aggaaattt aaatttttg ggcacttta 
        aagaactat attttttcc aaactccgc aaagagaag 
        tacaattta gacgattct cctccacct gcagtcagt 
        atcgtgtct tacgaccaa cgtggtttc gaagatgcc 
        gaaaaagca ctcgatagt gaatgttcc cagactata 
        aaactcgac cccagaatt ctacaagcc ttaaaaact 
        gccttaaat tccaaaatc aacgatccc aaaccgaac 
        gcaaacttg gaacttttg gacattgta gaacgcact 
        ttcgcccgc caaatggac atcttcgaa ggcctcctc 
        aagcacatc aactccaag agcgaatcg gacaaagtc 
        aagttgcaa agaaagcta cggtcatca ctcaaagat 
        cgatcgctc gatttgaat tacattatt aataattta 
        gtaacggag ctgaacgcg aactcaaac gcccagcaa 
        atcaccatt gccttaaac gacgatacg ccaaatccc 
        ggggataat ccaaaaatc aaaaaaccg tcaattgag 
        tgtgagtgc cgccaggcc ggagccagt ggaggtgcc 
        gtaccaaga acccaataa
      mRNA sequence
      • >TC000450 RA
        ggaattctt tattagttc atgcttttt tcgactatt 
        cgactattt ttataatct taacgcaaa caataagga 
        ataaacaga atcacgtta tctcgtgtc gtttcgttt 
        tattatttt tccttctct ttgagtatt ttgagtgga 
        tttacaaat gtggaatta cttgacggc tgggggtaa 
        aagaacctt cgaggacaa agcaattca gacgattat 
        ccaacgcaa atgcggtgg tgacgtgtc tctgaaacc 
        tgaccaaga ggaacaagt cttttacaa gcttctgga 
        gtacactcc attcagaga cattcaaga agaaagcta 
        caaaatcgt cattttacc gagacctaa gcaggaaaa 
        tctgccgtc atcgattta cttaaactt ggagaactg 
        tcttttgat tgacgatga tgcgatcag tgagttgtg 
        tctggaact aagggagcg aattcgtaa agggtacaa 
        gcagttgaa aacttacaa tgacatggg aagtcaaac 
        tgatttaca cgtccaacc ccatatttt agaggatga 
        gaaagttgc aagcgttgt tgcggactg tgcttgttt 
        tgtgttatt aatggacaa atctgtacc caccatcag 
        ctctggagc agacctgga tgaggagaa atcaaagtt 
        tagggaaac ccttgctag gaaatttaa atttttggg 
        cactttaaa gaactatat tttttccaa actccgcaa 
        agagaagta caatttaga cgattctcc tccacctgc 
        agtcagtat cgtgtctta cgaccaacg tggtttcga 
        agatgccga aaaagcact cgatagtga atgttccca 
        gactataaa actcgaccc cagaattct acaagcctt 
        aaaaactgc cttaaattc caaaatcaa cgatcccaa 
        accgaacgc aaacttgga acttttgga cattgtaga 
        acgcacttt cgcccgcca aatggacat cttcgaagg 
        cctcctcaa gcacatcaa ctccaagag cgaatcgga 
        caaagtcaa gttgcaaag aaagctacg gtcatcact 
        caaagatcg atcgctcga tttgaatta cattattaa 
        taatttagt aacggagct gaacgcgaa ctcaaacgc 
        ccagcaaat caccattgc cttaaacga cgatacgcc 
        aaatcccgg ggataatcc aaaaatcaa aaaaccgtc 
        aattgagtg tgagtgccg ccaggccgg agccagtgg 
        aggtgccgt accaagaac ccaataatg tattattaa 
        agttgcatt aaatttaaa ttaccgacc cattccgcg 
        cactttttg ctccggcag ctcaccgtc agatggccg 
        cgtcaattt gacaggtaa ccaaacaat cgggaaatc 
        gccagttta tgagtgagt cacagtgga taaaagtgc 
        agttagtgg tgttttgta catatttac aaattatct 
        atcgtccac aaaatgcgc taatcaggc attaaattg 
        cctcacgtc acggcattt tacataagg caacggaac 
        cagccaaca atcttgcag tgaaaaact acctgacct 
        tcttaactt atcgttgct taattaacg tataaataa 
        ctattgttt gaaaatggt gcgtttttc ggtggaaaa 
        tatgtgaac tggcgaaat gggtcactt ccggacgag 
        ggtgcaccc ctactgaca agatgagta ggggatact 
        acaacaccg gggccccct ttctgtatt cggtaggtt 
        acgcatgtt tagattagt caagtggtg tttttaaac 
        aaccgtgta ctaaagcta ctcaaaacc c