Status
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Gene information
General information
TC000440 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11234012..11247730
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000440
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000440 RA
      MCASVYSSL LLFTLTTIS TIFDTRMAT EGLPLVRQE 
      RGADDRGLL AGADFLSVF MSSSLRQAY LETTTTTTP 
      DPTTIPTTW QSDSSYTRA TRSTSLLTS PPLAQTNTE 
      DSSFYDTTL SAQLRKLEP SSDSSLNDG VVVAVSVSS 
      SVGRTLINK EGTTTTIPD QNLVASEEH PSLVAEANK 
      SEFSVEESE PITTNSEAA RDFVQGYPA SVQPLPRHF 
      NPPSDKLHH PIHQTVIYH NNPRDPNRA RSVSYSTVI 
      QSVPHPETE KDWGDGDKH DRHERHFES SFGNNLHSE 
      KAKFNESKW QDSGESPGF TTERNWEIR EKPYTPPRL 
      PTVYGKPEQ NYEVDESAS VMTNGRIHG IQPSPTLPS 
      KEEVSKKID DNQKVGYVV EGRNYRKYR VEERTSDGF 
      IVGEYGVVS HDDGSLRGV RYTADGTIS PRLIYDALM 
      KFLSL
    CDS sequence
    • >TC000440 RA
      atgtgtgcc tccgtatac tcctccctg ctccttttc 
      acccttacc accatctcc acgattttc gatacgagg 
      atggcaacc gagggcctg ccgttggtg cggcaggag 
      cgcggtgcc gacgacaga ggccttctg gcgggagcc 
      gacttcctc tcagtcttc atgagctcc tctcttcgc 
      caagcttac ctggagacc accacgacc acaaccccc 
      gacccgaca acaattccg acaacgtgg cagtcggat 
      agctcttac actcgcgcc actcgaagc acctcactg 
      ctgacgtcg cctcccctt gctcaaacc aacacagaa 
      gattctagt ttttacgat acgacacta agtgctcaa 
      ttacgtaaa ctcgaaccg tccagtgac tctagttta 
      aacgacggt gtggtcgtt gctgtgagt gtgtcttcg 
      tcggtagga aggacacta atcaacaaa gaaggcacg 
      accacgacg atcccagac cagaatctc gtcgcttcg 
      gaggaacac cccagctta gtagccgaa gcgaacaaa 
      agcgaattc agcgtcgaa gagagcgag ccgatcacc 
      accaacagc gaagcggcg agagatttc gtccagggg 
      taccctgca agtgtgcag cccctgccc cgacatttc 
      aaccctcct tcggacaag ttgcaccac cccatccac 
      caaaccgtc atctaccac aacaacccc agagaccca 
      aatcgggcc agatcggtg tcctacagc accgtcatc 
      cagtccgtg cctcatccc gagaccgag aaagactgg 
      ggcgacggt gacaagcac gaccgccac gagcgacac 
      ttcgaatcc tccttcggt aacaatcta cactccgaa 
      aaagcgaaa ttcaacgaa agcaaatgg caagacagt 
      ggcgaatcc ccaggtttc acgacggag cgcaattgg 
      gaaattcgg gaaaaaccc tacaccccg ccacggcta 
      cccaccgtc tacggcaag cccgagcaa aactacgag 
      gtcgacgaa tctgccagt gtcatgacc aacggccgc 
      attcacggg atacagccg agtccgacc ctgccgtcg 
      aaggaagaa gtcagcaag aagatcgac gataatcag 
      aaagtcggc tacgtggtc gaaggccgc aattacagg 
      aaatacaga gttgaggag cggacctcc gatgggttc 
      atcgtgggc gagtatggg gttgtgagc catgacgat 
      gggtctctg cgtggggtg cggtacacc gccgatgga 
      acgattagt cctaggtta atttacgac gcgctgatg 
      aaattcctg tcgttgtga
    mRNA sequence
    • >TC000440 RA
      tccggtcga ttgcagtcg ttgcgagca aagtgcttg 
      tgttgtttc agtgtctcg cgaagctcg ctggggcgc 
      ggacgggcg agtgagtgg cgcatgatg atgtgaatt 
      gcagacatg tgtgcctcc gtatactcc tccctgctc 
      cttttcacc cttaccacc atctccacg attttcgat 
      acgaggatg gcaaccgag ggcctgccg ttggtgcgg 
      caggagcgc ggtgccgac gacagaggc cttctggcg 
      ggagccgac ttcctctca gtcttcatg agctcctct 
      cttcgccaa gcttacctg gagaccacc acgaccaca 
      acccccgac ccgacaaca attccgaca acgtggcag 
      tcggatagc tcttacact cgcgccact cgaagcacc 
      tcactgctg acgtcgcct ccccttgct caaaccaac 
      acagaagat tctagtttt tacgatacg acactaagt 
      gctcaatta cgtaaactc gaaccgtcc agtgactct 
      agtttaaac gacggtgtg gtcgttgct gtgagtgtg 
      tcttcgtcg gtaggaagg acactaatc aacaaagaa 
      ggcacgacc acgacgatc ccagaccag aatctcgtc 
      gcttcggag gaacacccc agcttagta gccgaagcg 
      aacaaaagc gaattcagc gtcgaagag agcgagccg 
      atcaccacc aacagcgaa gcggcgaga gatttcgtc 
      caggggtac cctgcaagt gtgcagccc ctgccccga 
      catttcaac cctccttcg gacaagttg caccacccc 
      atccaccaa accgtcatc taccacaac aaccccaga 
      gacccaaat cgggccaga tcggtgtcc tacagcacc 
      gtcatccag tccgtgcct catcccgag accgagaaa 
      gactggggc gacggtgac aagcacgac cgccacgag 
      cgacacttc gaatcctcc ttcggtaac aatctacac 
      tccgaaaaa gcgaaattc aacgaaagc aaatggcaa 
      gacagtggc gaatcccca ggtttcacg acggagcgc 
      aattgggaa attcgggaa aaaccctac accccgcca 
      cggctaccc accgtctac ggcaagccc gagcaaaac 
      tacgaggtc gacgaatct gccagtgtc atgaccaac 
      ggccgcatt cacgggata cagccgagt ccgaccctg 
      ccgtcgaag gaagaagtc agcaagaag atcgacgat 
      aatcagaaa gtcggctac gtggtcgaa ggccgcaat 
      tacaggaaa tacagagtt gaggagcgg acctccgat 
      gggttcatc gtgggcgag tatggggtt gtgagccat 
      gacgatggg tctctgcgt ggggtgcgg tacaccgcc 
      gatggaacg attagtcct aggttaatt tacgacgcg 
      ctgatgaaa ttcctgtcg ttgtgatcg aaactcggt 
      gcaatactt gccttcgta ctgatttaa tctagttcc 
      tcccattaa actcggtct tatttcata gctaaacga 
      gacttgagt gaagtgaac tcgaactgt acttgtgct 
      aattattac aaagttgtc aagcttttt aataatcga 
      ctatttgct cataactat ttaataatt agtttattg 
      acgacatgt ttttatatt taactgtaa cgaaattct 
      gtaaattag gtaataaag ccttgcgaa aagtgttat 
      gtctatgga gcacgtcgt aatgtatgt acacgattg 
      tcagctata aacaacaat aaaacattt caagcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY