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Gene information
General information
TC000408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11836223..11870107
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000408
    iBeetle screen iB_03018

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000408 RA
      MPSLFAIGD HRLTTVLTM ASLGSGRLC ARVQEKLCQ 
      WGQSAQFRR EASSCVRTK LGLQTTPRI VTKEHQNQR 
      QSQTLAAAA AQSVSSGCW HPCSRPEVA ATARSFSTM 
      RTQQPGSLP EYQVDPYHL LEDDLKDVY VYIRDALRR 
      STYQDDLYE IATYYFDGH GKAIRPMVA ILMARAINY 
      HIYKEKSGL LTSQREVAM ISEMIHTAS LVHDDVIDQ 
      SDFRRGKPS VNVMWNHKK VTMAGDFIL AVASIMIAR 
      LKNNDVTLV LSQVVSDLV QGEFMQLGS KETENERFA 
      HYLTKTYRK TASLIANSV KSVTMLAGA DEQLSDISY 
      QYGRNLGLA FQLVDDLLD FVSSSAAMG KPTAADLKL 
      GLATAPVLF ACEKFPELN PMIMRRFQE PGDVERAFD 
      LVHKSRGLE QTQYLAKQH CQEAVRLAN LLEESPYQK 
      GLIVAADYV LNRMK
    CDS sequence
    • >TC000408 RA
      atgccgagc ctgtttgcg ataggggac caccgtctc 
      acaacagtc ttgacgatg gcttctcta ggctctggt 
      agactctgt gctcgggtg caggaaaaa ctgtgtcag 
      tggggacaa tcggctcaa ttcaggagg gaagcgtcc 
      tcctgtgtg cggacaaaa ctcggattg caaaccacc 
      cccaggata gtgaccaag gagcaccag aaccagcga 
      caatcacag actttggcg gctgctgcg gcacagagt 
      gtgtcttca ggatgttgg catccttgc agcaggccg 
      gaagttgca gccacagcg cgcagtttc agcacgatg 
      agaacgcag caacctggt tccctcccc gaataccaa 
      gtggacccc tatcacctt ttagaagac gacctcaag 
      gacgtttac gtgtatatc cgagacgct ttgcgaaga 
      agcacttac caagatgac ctctacgag atagctacg 
      tattatttc gatggacat gggaaagca attagacca 
      atggtggct atcctcatg gctagggcg ataaattat 
      cacatatat aaagaaaaa agtggtctt ttgacgtcg 
      caaagggaa gtagcgatg atttcggaa atgatccac 
      acagcctct ctcgtccac gacgacgtc atcgatcag 
      tcagatttc cgtcgtggt aaacccagc gtcaacgtg 
      atgtggaat cacaaaaag gtgaccatg gctggagat 
      ttcatctta gcggttgcc tcaatcatg atagctagg 
      ttaaaaaat aacgacgtc actctagtt cttagtcaa 
      gttgtctcc gacttagtt cagggagag ttcatgcaa 
      ctcggatct aaggaaacg gaaaacgaa cgatttgct 
      cattatcta accaaaacg tacagaaaa accgcttct 
      ctgatagcc aactctgta aaatctgtg acaatgtta 
      gccggtgct gatgagcaa ctctccgac atttcgtac 
      caatacgga cgcaattta ggtttagcg tttcagtta 
      gttgacgat cttctcgat tttgtctcg agttctgca 
      gccatggga aagcccaca gcggccgac ctgaagctg 
      ggcctggcc acagcgccc gtgctcttc gcttgcgaa 
      aaattccct gaactgaac ccgatgata atgcggcgg 
      ttccaagag cctggggac gtggaaagg gcgtttgac 
      ctcgtccac aaatcgcga gggctggag cagacgcag 
      tatttggcc aagcagcac tgccaggag gccgtgagg 
      cttgcaaac cttctggag gagagtccg taccaaaaa 
      ggactgata gttgcggct gattatgta ctaaaccgc 
      atgaaatag
    mRNA sequence
    • >TC000408 RA
      ggaaatctt ctgcccaag acggaggcg atggccaat 
      tcgagccgt tgtattgcg acatttaag cggattaaa 
      agtgaaccg gccatggtt taggagcag cagcgtttt 
      ccgatcagc tgtagtggg acaaactgg tggaaaggt 
      gcgatgccg agcctgttt gcgataggg gaccaccgt 
      ctcacaaca gtcttgacg atggcttct ctaggctct 
      ggtagactc tgtgctcgg gtgcaggaa aaactgtgt 
      cagtgggga caatcggct caattcagg agggaagcg 
      tcctcctgt gtgcggaca aaactcgga ttgcaaacc 
      acccccagg atagtgacc aaggagcac cagaaccag 
      cgacaatca cagactttg gcggctgct gcggcacag 
      agtgtgtct tcaggatgt tggcatcct tgcagcagg 
      ccggaagtt gcagccaca gcgcgcagt ttcagcacg 
      atgagaacg cagcaacct ggttccctc cccgaatac 
      caagtggac ccctatcac cttttagaa gacgacctc 
      aaggacgtt tacgtgtat atccgagac gctttgcga 
      agaagcact taccaagat gacctctac gagatagct 
      acgtattat ttcgatgga catgggaaa gcaattaga 
      ccaatggtg gctatcctc atggctagg gcgataaat 
      tatcacata tataaagaa aaaagtggt cttttgacg 
      tcgcaaagg gaagtagcg atgatttcg gaaatgatc 
      cacacagcc tctctcgtc cacgacgac gtcatcgat 
      cagtcagat ttccgtcgt ggtaaaccc agcgtcaac 
      gtgatgtgg aatcacaaa aaggtgacc atggctgga 
      gatttcatc ttagcggtt gcctcaatc atgatagct 
      aggttaaaa aataacgac gtcactcta gttcttagt 
      caagttgtc tccgactta gttcaggga gagttcatg 
      caactcgga tctaaggaa acggaaaac gaacgattt 
      gctcattat ctaaccaaa acgtacaga aaaaccgct 
      tctctgata gccaactct gtaaaatct gtgacaatg 
      ttagccggt gctgatgag caactctcc gacatttcg 
      taccaatac ggacgcaat ttaggttta gcgtttcag 
      ttagttgac gatcttctc gattttgtc tcgagttct 
      gcagccatg ggaaagccc acagcggcc gacctgaag 
      ctgggcctg gccacagcg cccgtgctc ttcgcttgc 
      gaaaaattc cctgaactg aacccgatg ataatgcgg 
      cggttccaa gagcctggg gacgtggaa agggcgttt 
      gacctcgtc cacaaatcg cgagggctg gagcagacg 
      cagtatttg gccaagcag cactgccag gaggccgtg 
      aggcttgca aaccttctg gaggagagt ccgtaccaa 
      aaaggactg atagttgcg gctgattat gtactaaac 
      cgcatgaaa tagtacacg agggtagta cttgtgttg 
      agtaggtgt tctagttat gaaatttac ttcgatagg 
      tgacgttag ttgtatatt tttgtattt gtatgacta 
      tacagctcg atatttatc acatcggca tttcaagac 
      cagtgtctt ctatgttag aaaatttga tattattac 
      actttgaat aaactagtt gttactgtt gtgtgtgta 
      agtgtgtga accaaaaaa cgaatgagt cactactgt 
      tataaatat ccagtcatg cctaggata agattttga 
      tgatggtat aacgcttac atttactgc caggattta 
      tttttattt attcagcat acctacaca ggggttatt 
      tattttgat tcgttaata aaatgttaa aagaagtaa 
      tttggtgtc tgttttaaa ccgatagtt gctagtagg 
      tgcgctcaa tagatttga atatgtttc atgtatata 
      attttaaaa gtattaaag atatttatg ttaaaaata 
      atatattac ttacttgta attttgttg atgaatctg 
      atgtatata agttcttta acaagtgtt attattccg 
      tcaataatt acgagcaat gtttgcaca aatgatgta 
      tataagcag ttttgttac aattattat gtaaaatat 
      gatatctaa tatgtatat tttgattta tctacatgt 
      aaaatttat taataaagt gaacaactg gtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY