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Gene information
General information
TC000399 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6499033..6501104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000399
    iBeetle screen iB_03016
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000399 RA
      MAEETDKKQ PLSYYYETF ELDTYFTQN KIKIPQVKK 
      DGFSFRKLW AFTGPGFLM SIAYLDPGN IESDLQSGA 
      IGRYRLLWV LFAATILGY LLQSLASKL GVVTGLHLA 
      EMCYRQYRK FPRLVLWIM VEIAVIGSD MQEVIGTSI 
      AIYLLSNKL IPLWAGCLI TIADTFTFL FLDRYGLRK 
      LELFFGILI TIMGLTFGY EYVVSVPPQ DAIMKGLFI 
      PWCEGCDAN VLLQAVGVI GAVLMPHNL YLHSALVKS 
      REVDRSRPE KIKEARMYY MLENAIALV CSFIINIFV 
      VTVFAYGLF EKTNNEILN NCPKNESLV YDRAVEVFP 
      RDDEYVDAD IYKGGVYLG CAYGPAAMY IWAIGILAA 
      GQSSTMTGT YAGQFAMEG FLDLQWARW KRVLFTRSI 
      AMIPTFFVA FFSSLNELT TMNDFLNAV MSLQLPFAI 
      IPMVAFTSN PKIMGEFVN GLGMRVVFT ILGVVIIGI 
      NIFFVTDTL IELNINSYY YIAIGIAAA LYFIFVLYL 
      ALHCYISFG GKKIEQRPW VQKYVVYAE MNTPKRNV
    CDS sequence
    • >TC000399 RA
      atggccgaa gaaaccgat aaaaaacaa cccttgagc 
      tattattac gagaccttc gaattggac acgtacttt 
      actcaaaac aaaataaaa atacctcaa gtgaaaaag 
      gatggattt tcttttcgc aaattgtgg gcatttaca 
      gggcccgga tttctaatg tctattgct tacttagat 
      ccgggaaat atcgagagt gatcttcag tccggtgct 
      attggacgg tatagatta ctttgggtt ttgttcgct 
      gccactatt ttaggatat ttattgcaa agtcttgcc 
      tctaaatta ggggttgtg actgggctg cacttggcc 
      gaaatgtgc tacagacag taccgaaaa ttcccgcgg 
      ttggttctg tggattatg gtggaaata gcggtaatt 
      gggtcagat atgcaggaa gtgatcggg acttcaatt 
      gccatttat ttgctctca aataaattg atacctttg 
      tgggctggc tgtcttata actatagcc gacactttt 
      acctttctc ttcttggat cgctacggt ttgagaaag 
      ttggaactg ttttttggg attttaatc acaataatg 
      ggtctgacg tttgggtac gagtacgta gtgtctgtt 
      cccccacag gacgcaata atgaaaggg ttgtttatc 
      ccttggtgt gaaggttgc gacgctaat gtgctgctc 
      caggccgtc ggagtgatc ggtgctgtt ctaatgccc 
      cacaatctc tacctgcac tccgcttta gtcaagtcc 
      cgcgaagtc gaccggagc cgcccggaa aaaatcaaa 
      gaagctcgg atgtactac atgctcgaa aacgccatc 
      gcccttgtt tgcagtttc attataaac atatttgtc 
      gtgacggtt ttcgcttac ggactgttc gaaaaaacc 
      aacaatgaa attttgaac aattgcccc aaaaatgaa 
      tctcttgtt tacgaccga gccgttgaa gtttttccg 
      agggatgac gaatacgtg gacgcggac atttataaa 
      gggggagtt tatttagga tgtgcttac ggcccggct 
      gctatgtac atatgggct attgggatt ttggccgca 
      gggcagagc tcgacgatg acaggaaca tacgcagga 
      cagttcgcc atggaaggg ttcctggac ctgcaatgg 
      gcgcgctgg aagcgagtt ctattcaca cggagcatc 
      gccatgatc cccactttc ttcgtcgct tttttcagc 
      tctctaaac gaactcact acaatgaac gacttcctt 
      aatgccgtc atgagcctg cagctgccc ttcgccatt 
      atcccgatg gtcgccttc accagcaat cccaaaatt 
      atgggcgaa ttcgtaaac ggcctgggc atgagggtc 
      gtgttcaca atcctcggc gttgtcata attggaatt 
      aacattttt ttcgttact gacactctc attgaactc 
      aacattaac agttattat tacattgcc atcgggata 
      gcggcggct ctttatttt atttttgtc ctgtatctg 
      gccctgcat tgttatata tctttcggt ggcaaaaaa 
      atcgagcaa cggccctgg gtccaaaaa tatgtggtg 
      tatgccgaa atgaatact ccgaaacga aacgtgtga
    mRNA sequence
    • >TC000399 RA
      tagttagtt tgcgaaaat ccagtgcgt ccaattttc 
      gcccaaaat ggccgaaga aaccgataa aaaacaacc 
      cttgagcta ttattacga gaccttcga attggacac 
      gtactttac tcaaaacaa aataaaaat acctcaagt 
      gaaaaagga tggattttc ttttcgcaa attgtgggc 
      atttacagg gcccggatt tctaatgtc tattgctta 
      cttagatcc gggaaatat cgagagtga tcttcagtc 
      cggtgctat tggacggta tagattact ttgggtttt 
      gttcgctgc cactatttt aggatattt attgcaaag 
      tcttgcctc taaattagg ggttgtgac tgggctgca 
      cttggccga aatgtgcta cagacagta ccgaaaatt 
      cccgcggtt ggttctgtg gattatggt ggaaatagc 
      ggtaattgg gtcagatat gcaggaagt gatcgggac 
      ttcaattgc catttattt gctctcaaa taaattgat 
      acctttgtg ggctggctg tcttataac tatagccga 
      cacttttac ctttctctt cttggatcg ctacggttt 
      gagaaagtt ggaactgtt ttttgggat tttaatcac 
      aataatggg tctgacgtt tgggtacga gtacgtagt 
      gtctgttcc cccacagga cgcaataat gaaagggtt 
      gtttatccc ttggtgtga aggttgcga cgctaatgt 
      gctgctcca ggccgtcgg agtgatcgg tgctgttct 
      aatgcccca caatctcta cctgcactc cgctttagt 
      caagtcccg cgaagtcga ccggagccg cccggaaaa 
      aatcaaaga agctcggat gtactacat gctcgaaaa 
      cgccatcgc ccttgtttg cagtttcat tataaacat 
      atttgtcgt gacggtttt cgcttacgg actgttcga 
      aaaaaccaa caatgaaat tttgaacaa ttgccccaa 
      aaatgaatc tcttgttta cgaccgagc cgttgaagt 
      ttttccgag ggatgacga atacgtgga cgcggacat 
      ttataaagg gggagttta tttaggatg tgcttacgg 
      cccggctgc tatgtacat atgggctat tgggatttt 
      ggccgcagg gcagagctc gacgatgac aggaacata 
      cgcaggaca gttcgccat ggaagggtt cctggacct 
      gcaatgggc gcgctggaa gcgagttct attcacacg 
      gagcatcgc catgatccc cactttctt cgtcgcttt 
      tttcagctc tctaaacga actcactac aatgaacga 
      cttccttaa tgccgtcat gagcctgca gctgccctt 
      cgccattat cccgatggt cgccttcac cagcaatcc 
      caaaattat gggcgaatt cgtaaacgg cctgggcat 
      gagggtcgt gttcacaat cctcggcgt tgtcataat 
      tggaattaa cattttttt cgttactga cactctcat 
      tgaactcaa cattaacag ttattatta cattgccat 
      cgggatagc ggcggctct ttattttat ttttgtcct 
      gtatctggc cctgcattg ttatatatc tttcggtgg 
      caaaaaaat cgagcaacg gccctgggt ccaaaaata 
      tgtggtgta tgccgaaat gaatactcc gaaacgaaa 
      cgtgtgact aaataaatt tttccttcg taa